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51.
Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding. 相似文献
52.
Zhi-gen Yang Wen-yao Zhu C. K. Shum Feng-chun Shu 《Chinese Astronomy and Astrophysics》2002,26(4):303-502
The vertical deformation rates (VDRs) and horizontal deformation rates (HDRs) of Shanghai VLBI station in China and Kashima and Kashima34 VLBI stations in Japan were re-analysed using the baseline length change rates from Shanghai to 13 global VLBI stations, and from Kashima to 27 stations and from Kashima34 to 12 stations, based on the NASA VLBI global solution glb1123 (Ma, 1999). The velocity vectors of the global VLBI stations were referred to the ITRF97 reference frame, and the Eulerian vectors of different models of plate motion were used for comparative solutions. The VDR of Shanghai station is estimated to be −1.91±0.56 mm/yr, and those of Kashima and Kashima34 stations, −3.72±0.74mm/yr and −8.81±0.84mm/yr, respectively. The difference between the last two was verified by further analysis. Similar estimates were also made for the Kokee, Kauai and MK_VLBA VLBI stations in mid-Pacific. 相似文献
53.
The effects of natural fish oil,DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream(Sparus aurata)were investigated after 15 days feeding trials.The tolerance of larval gilthead seabream to various stress factors such as exposure to air(lack of dissolved oxygen),changes in water temperature(low)and salinity(high) were determined.This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for in-creasing the tolerance of larval gilthead seabream to various stresses,and that microparticulate diet with natural fish oil and palmitic acid(16:0)was more effective than microparticulate diet with DHA oil and soybean lecithin. 相似文献
54.
青藏高原隆升的非线性动态有限元仿真研究 总被引:7,自引:4,他引:3
根据青藏高原的地质特征建立分析模型,采用3维动态有限元方法,在计算仿真板块速度场的基础上,计算在青藏高原的隆升过程中该地区地壳岩石的等效应力和位移随时间的变化,计算仿真得到的速度场与1998年GPS观测的速度场吻合良好;与过去一贯的假设相反,计算结果反映出地壳应力场不是静态的,而是此起彼伏,不断变化的,应力值最大且变化最剧烈的地区在克什米尔地区、鄂尔多斯地区和鲜水河-小江断裂带,与地震多发区域吻合。 相似文献
55.
AN ILLUSTRATIVE STUDY ON LOCAL LANDSCAPEAND ITS LONG-TERM CHANGES BASED ON IKONOS AND HISTORICAL AERIAL PHOTO 总被引:2,自引:0,他引:2
RUANRen-zong ErieCELLISt 《中国地理科学(英文版)》2004,14(2):162-169
Nowadays, the research works on landscape at fine scales using high-resolution images are uncommon.This research is based on the analysis of the combination of remote sensing data (1KONOS imagery acquired in 2002 and historical aerial photo taken in 1942). In the paper, the ecotopes in Qiujiadou and Xishao villages in Yixing City of Jiangsu Province in 1942 and 2002 were compared and landscape changes as well as the causes of the considerable changes were analyzed. It was found that the ecotope changes were at greater level in some aspects such as water surface and perennial vegetation coverage etc. This study at fine scale is globally significant for the rural areas, especially for the subsistence agricultural land, which occupies larger percentage in the earth. And it analyzes the structure of landscape based on a new landscape classification system--stratifications method. 相似文献
56.
A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars. 相似文献
57.
中国1∶100万景观生态制图设计 总被引:5,自引:0,他引:5
中国1∶100万景观生态图系借鉴国内外传统景观制图方法和制图规范,在遥感、地理信息系统先进技术的支持下,设计研制其制图方法、制图内容、样图和技术流程,同时提出初步的景观分类系统,为今后编制中国1∶100万景观生态图奠定基础。 相似文献
58.
TANGYa XIEJiasui SUNHui 《山地科学学报》2004,1(1):38-45
Dry valleys are a striking geographic landscape in Hengduan Mountains Region and are characterized by low rainfall, desert type of vegetation and fragile environment. Past efforts and resources have been concentrated mainly on rehabilitation of degraded ecosystem and fragile environment, particularly reforestation, while socio-economic development has been largely overlooked. Despite successes in pocket areas, the overall trend of unsustainability and environmental deterioration are continuing. It is important to understand that uplift of the Tibetan Plateau is the root cause of development of dry valleys, and development and formation of dry valleys is a natural process. Human intervention has played a secondary role in development of dry valleys and degradation of dry valleys though human intervention in many cases has speeded up environmental degradation of the dry valleys. It is important to understand that dry valleys are climatic enclaves and an integrated approach that combines rehabilitation of degraded ecosystems and socio-economic development should be adopted if the overall goal of sustainable development of dry valleys is to be achieved. Promotion of niche-based cash crops, rural energy including hydropower, solar energy, biogas and fuelwood plantation is recommended as the priority activities. 相似文献
59.
为了便于大型软件系统的后续开发和用户软件系统的升级,介绍了软件系统在Internet/Intranet上更新功能或数据的原理和方法,并用C#.Net进行了编程实现。 相似文献
60.
Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk
thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only
the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated
fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs
was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae,
and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs
or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments
containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally
as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it
was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage
of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible
for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of
the starfish had already regionalized before the GV break-down.
Contribution No. 1722 from the Institute of Oceanology, Academia Sinica 相似文献