Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza     

吕明生  蔡阮鸿  王淑军  刘兆普  焦豫良  房耀维  张小昕 《中国海洋湖沼学报》2013,31(6):1190-1195

A superoxide dismutase was purified from Enteromorpha linza using a simple and safe procedure, which comprised phosphate buffer extraction, ammonium sulphate precipitation, ion exchange chromatography on Q-sepharose column, and gel filtration chromatography on Superdex 200 10/300GL. The E. linza superoxide dismutase （E/SOD） was purified 103.6-fold, and a yield of 19.1% and a specific activity of 1 750 U/rag protein were obtained. The SDS-PAGE exhibited E/SOD a single band near 23 kDa and the gel filtration study showed E/SOD＇s molecular weight is near 46 kDa in nondenatured condition, indicating it＇s a homodimeric protein. E/SOD is an iron-cofactored superoxide dismutase （Fe-SOD） because it was inhibited by hydrogen peroxide, insensitive to potassium cyanide. The optimal temperature for its maximal enzyme activity was 35℃, and it still had 29.8% relative activity at 0℃, then E/SOD can be classified as a cold-adapted enzyme. E/SOD was stable when temperature was below 40℃ or the pH was within the range of 5 10. The first 11 N-terminal amino acids orE/SOD were ALELKAPPYEL, comparison of its N-terminal sequence with other Fe-SOD N-terminal sequences at the same position suggests it is possibly a chloroplastic Fe-SOD.  相似文献   

除草剂草丁膦抗性基因bar作为三角褐指藻基因工程选择标记的研究     

卞曙光  姜鹏  崔玉琳  刘兆普  秦松 《海洋科学》2010,34(5):62-66

研究了三角褐指藻(Phaeodactylum tricornutum)对氯霉素、卡那霉素、青霉素、链霉素、潮霉素5种抗生素和一种除草剂草丁膦的敏感性,发现三角褐指藻对草丁膦非常敏感,用统计学分析法得出了其72h半致死质量浓度为22mg/L。通过基因枪法将携带草丁膦抗性基因bar的载体pSVB导入三角褐指藻细胞中,通过草丁膦筛选成功获得了抗性藻细胞,PCR检测获得阳性结果,提示草丁膦更适合用于构建安全、高效、廉价的三角褐指藻表达系统。  相似文献   

周期性饥饿再投喂对建鲤（Cyprinus carpio var．Jian）生长、体组成、消化酶的影响          下载免费PDF全文

乔秋实  蒋广震  刘文斌  夏薇  刘兆普 《海洋与湖沼》2011,42(3):367-373

采用周期性饥饿再投喂的方法,研究周期性饥饿再投喂对建鲤（1．5±0．2g）的生长、体组成、消化酶的影响。试验分为10个组,共进行60d。结果表明,在相同的饥饿天数下,延长再投喂天数可以使建鲤的增重率逐渐增加,而且S1F4、S2F8、S4F16组的增重率与对照组无显著性差异（P〉0．05）,9个周期性饥饿再投喂组在摄食期...  相似文献   

三峡库区稻田复养河蟹的农田生态系统能量特征与经济效益   总被引：1，自引：0，他引：1  

陈铭达  刘兆普  刘文斌  王景艳  叶志娟 《山地学报》2005,23(4):469-475

在2002年四川南充稻田养蟹田间探索性试验的基础上,2003年在位于三峡库区淹没带边缘非淹区的重庆市开县竹溪镇进行稻田复养河蟹的田间试验,对种、养结合模式农田生态系统的能流与物流进行了初步探索,并进行了经济效益分析.结果表明：稻蟹复养田的光能利用率高于单作水稻田的15.7%;水稻生长期间的防病治虫用药、运作管理用工、无机肥用量、机械耗材的投入成本明显减少,总的投入比单作水稻田少4.3%,总能、无机能、有机能的产投比分别少35.1%、51.0%和31.6%;土壤全氮、氨态氮分别高于单作水稻田的21.4%和55.2%,稻田水中的氨态氮高达61.3%,这些无机态氮是培育水生生物、高等植物的营养源,又是供应河蟹生长的饵料,而硝态氮则要低得多;河蟹的甲壳质及其排泄物经分解矿化后,是提高土壤有效养分,供应水稻生长的主要肥源.稻田养蟹后,虽然稻谷产量比单作水稻低10.1%,投入成本且低于20.0%,而利润比单作水稻增加77.8%;河蟹的产投比1.54：1,获利润6 381.49元/hm^2;稻田复养河蟹的净利润为7104.19元/hm^2,是单作水稻的44.3倍.  相似文献