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Chinese shrimp(Fenneropenaeus chinensis)is an economically important aquaculture species in China.However,cytogenetic and genomic data is limited in the organism partly because the chromosomes are difficult to isolate and analyze.In this study,fluorescence in-situ hybridization(FISH) was used to identify the chromosomes of F.chinensis.The 5S ribosomal RNA gene(rDNA)of F. chinensis was isolated,cloned and then used as a hybridization probe.The results show that the 5S rDNA was located on one pair of homologous chromosomes in F.chinensis.In addition,triploid shrimp were used to evaluate the feasibility of chromosome identification using FISH and to validate the method.It was confirmed that 5S rDNA can be used as a chromosome-specific probe for chromosome identification in F.chinensis.The successful application of FISH in F.chinensis shows that chromosome-specific probes can be developed and this finding will facilitate further research on the chromosomes of penaeid shrimps. 相似文献
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基于数据流量的统计功能是评判数据产地应用服务的一项指标;统计不同类型观测数据的使用、共享情况,可为分析数据的使用需求提供参考。因此,设计观测数据流量统计系统具有较强的应用价值。基于中国科学院国家天文台怀柔太阳观测基地的数据服务,以LAMP为开发环境,设计了数据流量实时统计及定时入库、数据流量查询功能、流量可视化功能(包括直方图显示和饼状图显示)等功能模块,以流量统计层、数据层、业务层及用户层4个层次为结构框架,实现了统计及查询任意时段内多种仪器观测的多种数据传输至各用户单位的流量功能,系统具有统计精确、多个关键字进行完整查询、显示直观、扩展性强等优点,能够满足当前及未来应用需求,对其它数据产地的多类型数据的流量统计具有可用性。 相似文献