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61.
The initial sampling in the Marine Monitoring Program (MOMAM), coordinated by the Ministry of Marine Affairs (IEAPM), was performed along the southeast coast of Brazil. Orthopristis ruber samples were collected at Guanabara, Sepetiba and Ilha Grande Bays. Microsomal CYP1A levels and cytosolic cholinesterase (ChE), catalase (CAT) and glutathione S-transferase (GST) activities were measured in the liver of these fish according to established procedures. CAT activity and CYP1A content were significantly higher (P < or = 0.05) in fish caught at Guanabara Bay, which might be due to higher levels of peroxisome proliferators and Ah receptor agonists, respectively, at this site compared to the other sites. Also, lower GST activity was observed in fish from this site, which may possibly be related to the presence of oxidative-stress inducing compounds.  相似文献   
62.
Carbon isotopically based estimates of CO2 levels have been generated from a record of the photosynthetic fractionation of 13C [is equivalent to epsilon(p)] in a central equatorial Pacific sediment core that spans the last approximately 255 ka. Contents of 13C in phytoplanktonic biomass were determined by analysis of C37 alkadienones. These compounds are exclusive products of Prymnesiophyte algae which at present grow most abundantly at depths of 70-90 m in the central equatorial Pacific. A record of the isotopic composition of dissolved CO2 was constructed from isotopic analyses of the planktonic foraminifera Neogloboquadrina dutertrei, which calcifies at 70-90 m in the same region. Values of epsilon(p), derived by comparison of the organic and inorganic delta values, were transformed to yield concentrations of dissolved CO2 [is equivalent to c(e)] based on a new, site-specific calibration of the relationship between epsilon(p) and c(e). The calibration was based on reassessment of existing epsilon(p) versus c(e) data, which support a physiologically based model in which epsilon(p) is inversely related to c(e). Values of PCO2, the partial pressure of CO2 that would be in equilibrium with the estimated concentrations of dissolved CO2, were calculated using Henry's law and the temperature determined from the alkenone-unsaturation index U(K/37). Uncertainties in these values arise mainly from uncertainties about the appropriateness (particularly over time) of the site-specific relationship between epsilon(p) and 1/c(e). These are discussed in detail and it is concluded that the observed record of epsilon(p) most probably reflects significant variations in delta pCO2, the ocean-atmosphere disequilibrium, which appears to have ranged from approximately 110 microatmospheres during glacial intervals (ocean > atmosphere) to approximately 60 microatmospheres during interglacials. Fluxes of CO2 to the atmosphere would thus have been significantly larger during glacial intervals. If this were characteristic of large areas of the equatorial Pacific, then greater glacial sinks for the equatorially evaded CO2 must have existed elsewhere. Statistical analysis of air-sea pCO2 differences and other parameters revealed significant (p<0.01) inverse correlations of delta pCO2 with sea surface temperature and with the mass accumulation rate of opal. The former suggests response to the strength of upwelling, the latter may indicate either drawdown of CO2 by siliceous phytoplankton or variation of [CO2]/[Si(OH)4] ratios in upwelling waters.  相似文献   
63.
Urbanization trends in Papua New Guinea are analyzed using official data from the 1971 and 1980 censuses. The author suggests that the massive urban population growth rate for 1966-1971 was a figment of post-enumeration miscalculation. However, the data indicate that the annual urban growth rate for the period 1971-1980 was nearly four times higher than the growth rate for the population as a whole.  相似文献   
64.
Oil pollution is a significant conservation concern. We examined data from six institutions along the coast of South America: Emergency Relief Team of the International Fund for Animal Welfare, Fundación Mundo Marino, Centro de Recupera??o de Animais Marinhos, Natura Patagonia, Associa??o R3 Animal, and Mar del Plata Aquarium and data from resightings in Argentina, Brazil, Chile and Falkland/Malvinas Islands. From 2000 to 2010, 2183 oiled Magellanic penguins were rehabilitated as part of the routine activities of these institutions or during emergency responses to eight oil spills in which they were involved; all rehabilitated penguins were flipper banded and released. Since their release, 41 penguins were resighted until 31 December 2011. The results demonstrate that, when combined with other prevention strategies, the rehabilitation of Magellanic penguins is a strategy that contributes to the mitigation of adverse effects of oil spills and chronic pollution to the species.  相似文献   
65.
The West Indian manatee Trichechus manatus is threatened with extinction in Brazil, and this study focused on nondestructive blood samples analyzed for metals, polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs), as well as biochemical and hematological biomarkers. Studied manatees were kept at Projeto Peixe-Boi headquarters in Pernambuco State, and at two natural areas in estuaries where they are released to the wild. Manatees kept at the natural estuary in Paraiba State have blood concentrations of Al, Pb, Cd, Sn that are 11, 7, 8 and 23 times greater, respectively, than the concentrations found in blood of animals from the same species in Florida, USA. An inhibition of butyrylcholinesterase in manatees kept at the two reintroduction sites in Alagoas and Paraiba States indicated possible exposure of the animals to cholinesterase inhibitor insecticides. PCBs and OCPs were not detected. Results from this study will help delineate conservation efforts in the region.  相似文献   
66.
A Monte Carlo-based approach to assess uncertainty in recharge areas shows that incorporation of atmospheric tracer observations (in this case, tritium concentration) and prior information on model parameters leads to more precise predictions of recharge areas. Variance-covariance matrices, from model calibration and calculation of sensitivities, were used to generate parameter sets that account for parameter correlation and uncertainty. Constraining parameter sets to those that met acceptance criteria, which included a standard error criterion, did not appear to bias model results. Although the addition of atmospheric tracer observations and prior information produced similar changes in the extent of predicted recharge areas, prior information had the effect of increasing probabilities within the recharge area to a greater extent than atmospheric tracer observations. Uncertainty in the recharge area propagates into predictions that directly affect water quality, such as land cover in the recharge area associated with a well and the residence time associated with the well. Assessments of well vulnerability that depend on these factors should include an assessment of model parameter uncertainty. A formal simulation of parameter uncertainty can be used to delineate probabilistic recharge areas, and the results can be expressed in ways that can be useful to water-resource managers. Although no one model is the correct model, the results of multiple models can be evaluated in terms of the decision being made and the probability of a given outcome from each model.  相似文献   
67.
While tomographic inversion has been successfully applied to laboratory- and field-scale tests, here we address the new issue of scale that arises when extending the method to a basin. Specifically, we apply the hydraulic tomography (HT) concept to jointly interpret four multiwell aquifer tests in a synthetic basin to illustrate the superiority of this approach to a more traditional Theis analysis of the same tests. Transmissivity and storativity are estimated for each element of a regional numerical model using the geostatistically based sequential successive linear estimator (SSLE) inverse solution method. We find that HT inversion is an effective strategy for incorporating data from potentially disparate aquifer tests into a basin-wide aquifer property estimate. The robustness of the SSLE algorithm is investigated by considering the effects of noisy observations, changing the variance of the true aquifer parameters, and supplying incorrect initial and boundary conditions to the inverse model. Ground water flow velocities and total confined storage are used as metrics to compare true and estimated parameter fields; they quantify the effectiveness of HT and SSLE compared to a Theis solution methodology. We discuss alternative software that can be used for implementing tomography inversion.  相似文献   
68.
Mercury, with its reactive forms being the most deleterious for the trophic chains, has been identified as a major pollutant in a few confined bodies of the coastal zone. Due to feeding, burrowing, and bioirrigation activities, infauna are known to play a crucial role in the biogeochemical processes of contaminants. The main goal of the present study is to evaluate the effects of Hediste diversicolor bioturbation on mercury fluxes from estuarine sediments in a mesocosms laboratory experiment. Additionally, an attempt was made to establish a relationship between the amount of remobilised mercury to the water column and the degree of contamination of the sediments using a mercury contamination gradient. The present experiment demonstrated that the bioturbation activity caused by the gallery-diffuser H. diversicolor did not influence the remobilisation of mercury (in dissolved reactive forms) from the sediment to the water column. The concentration of dissolved reactive mercury in the water column also did not reflect the degree of contamination in the sediments. The results obtained were in accordance with the sediment characteristics of the Mondego Estuary and Ria de Aveiro (Portugal), since they are very rich in organic matter content, iron/manganese total and hydrous oxides. These physicochemical characteristics of the sediments may partially justify the retention of mercury by these systems, even when they are subjected to bioturbation. In conclusion, sediments with high organic matter content and a high concentration of iron/manganese hydrous oxides allowed for efficient retention of mercury, and the bioturbation process did not seem to affect the system. Another conclusion from this study is that the remobilisation of mercury by bioturbation to the water column is not a fast process, requiring more than 24h. This study constitutes an important work in the area of the effects of bioturbation on mercury remobilisation. Considering the toxicity of mercury for the biota, it is essential to evaluate the real magnitude of mercury processes occurring in estuarine systems in order to obtain essential information on metal behaviour.  相似文献   
69.
70.
We have cloned the full-length cDNA of the first member of a new cytochrome P450 (CYP) family from the Pacific oyster Crassostrea gigas. This new CYP gene was obtained based on an initial 331bp fragment previously identified among the list of the differentially expressed genes in oysters exposed to untreated domestic sewage. The full-length CYP has an open reading frame of 1500bp and based on its deduced amino acid sequence was classified as a member of a new subfamily, CYP356A1. A phylogenetic analysis showed that CYP356A1 is closely related to members of the CYP17 and CYP1 subfamilies. Semi-quantitative RT-PCR was performed to analyze the CYP356A1 expression in different tissues of the oyster (digestive gland, gill, mantle and adductor muscle). Results showed slightly higher CYP356A1 expression in digestive gland and mantle, than the other tissues, indicating a possible role of the CYP356A1 in xenobiotic biotransformation and/or steroid metabolism.  相似文献   
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