合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析     

姜铁民  张勇  黄洁  谢莉萍  吴移谋  张荣庆 《海洋科学》2003,27(4):28-34

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。  相似文献   

Full-range nonlinear analysis of fatigue behaviors of reinforced concrete structures by finite element method     

Song Yupu  Zhao Shunbo  Wang Ruimin  Li Shuyao 《海洋学报(英文版)》1994,13(1):143-154

Ｆｕｌｌ－ｒａｎｇｅｎｏｎｌｉｎｅａｒａｎａｌｙｓｉｓｏｆｆａｔｉｇｕｅｂｅｈａｖｉｏｒｓｏｆｒｅｉｎｆｏｒｃｅｄｃｏｎｃｒｅｔｅｓｔｒｕｃｔｕｒｅｓｂｙｆｉｎｉｔｅｅｌｅｍｅｎｔｍｅｔｈｏｄ￥ＳｏｎｇＹｕｐｕ；ＺｈａｏＳｈｕｎｂｏ；ＷａｎｇＲｕｉｍ...  相似文献   

太湖表面定振波的数值计算和最大熵谱分析   总被引：1，自引：0，他引：1  

逄勇  濮培民 《海洋与湖沼》1996,27(2):157-162

利用水动力学方程对太湖表面的定振波进行计算，算得定振波周期约为４５２ｍｉｎ，另外，利用１９９２年８月２９－３１日在太湖西山观测到的水位资料，采用最大熵谱法，分析太湖表面的定振波，得周期值约为４５０ｍｉｎ。计算和分析的周期值基本吻合，取熵谱分析结果得太湖表面的单节点定振波周期值为４５０ｍｉｎ。  相似文献   

Nonlinear tidal waves in a kind of estuary with gradually varying cross-section     

Du Yong  Chen Zongyong  Ye Anle 《海洋学报(英文版)》1990,9(1):37-50

-Nonlinear tidal waves in a kind of estuary are studied in the paper using one-dimensional nonlinear hydrody-namic equations with friction. The estuary has exponentially varying width B=B0 e-bx and uniform depth h. The one-dimensional hydrodynamic equations are solved by perturbation method. It was found that our solution included two special cases, Pelisenpeki's solution and Airy's solution. The former can be got by letting b=0 in our solutions, and the latter by setting 6 = 0 and f= 0 (f is linear frictional coefficient). In terms of the second-order solution, the physical mechanism of nonlinear tidal waves in estuaries with gradually varying cross-section is explored. It is shown that, under the assumption of linear friction coefficient, shallow water constituent waves consist of two parts, one is produced by shallow water nonlinear effect outside the estuary, the other is generated by shallow water nonlinear effect inside estuary. In addition, the physical mechanism of the residual tidal current and  相似文献   

Glaciomarine sedimentation and palaeo-glacial setting of Maxwell Bay and its tributary embayment, Marian Cove, South Shetland Islands, West Antarctica     

Ho Il Yoon  Myung Woo Han  Byong-Kwon Park  Jae-Kyung Oh  Soon-Keun Chang 《Marine Geology》1997,140(3-4):265-282

High-resolution (3.5 kHz and multi-channel) seismic profiles and piston cores were collected from Maxwell Bay and its tributary embayment, Marian Cove, in the South Shetland Islands, Antarctica, during the Korea Antarctic Research Program (1992/93 and 1995/96) to elucidate the glaciomarine sedimentation processes and recent glacial history of the area. Seismic data from Maxwell Bay reveal a rugged bay margin and flattened basin floor covered with well-stratified hemipelagic muds. On the base-of-slope, acoustically transparent debris flows occur, indicating downslope resedimentation of glaciomarine sediments. Despite the subpolar and ice-proximal settings of Marian Cove, the seafloor is highly rugged with a thin sediment drape, suggesting that much of the area has been recently eroded by glaciers. Sediment cores from the cove penetrated three distinct fining-upward lithofacies: (1) basal till in the lower part of the core, accumulated just seaward of the grounding line of the tidewater glacier; (2) interlaminated sand and mud in the middle part, deposited in ice-proximal zone by a combination of episodic subglacial meltwater inflow and iceberg dumping; and (3) pebbly mud in the upper part, deposited in ice-distal zone by both surface meltwater plume and ice-rafting from the glacier front. A reconstruction of the glacial history of these areas since the late glacial maximum shows an ice sheet filling Maxwell Bay in late Wisconsin time and grounding of the tidewater glacier in Marian Cove until about 1300 yr BP.  相似文献   

养殖对虾病毒性疾病的细菌并发症防治研究   总被引：1，自引：0，他引：1  

苏国成  陈金翠 《台湾海峡》1996,15(2):200-204

本文研究报道了发病对虾肝胰腺分离菌物敏感性，采用防台细菌人工感染方法进行药物饲料的筛选，在实验室及养殖池中进行药物饲料的防治效果试验。结果表明，在对虾病毒流行期间，采用药物饲料可有效防止对虾爆发性大量死亡，提高对虾存活率。  相似文献   

扇贝外寄生车轮虫（Trichodina　jadranica　Raabe，1958）的形态学研究　（原生动物：纤毛门：缘毛目）     

徐奎栋  类彦立  宋微波 《中国海洋大学学报(自然科学版)》1995,(3)

采用Ｗｉｌｂｅｒｔ蛋白银法及Ｃｈａｔｔｏｎ—Ｌｗｏｆｆ银浸法对一种附生于栉孔扇贝鳃丝上的寄生车轮虫进行了光镜水平下的研究。依据其形态学和纤毛图式特征，该种被鉴定为ＴｒｉｃｈｏｄｉｎａｊａｄｒａｎｉｃａＲａａｂｅ，１９５８．此为我国新记录，同时也是第一例有关外寄生于扇贝鳃表纤毛虫原生动物的报道。  相似文献   

Forearc structures and tectonics in the southern Peru—northern Chile Continental Margin     

Chang Li 《Marine Geophysical Researches》1995,17(1):97-113

SeaMARC II side-scan images, bathymetry, and single-channel seismic reflection data along the southern Peru—northern Chile forearc area between 16° and 23° S reveal a complex region of morpho-structural, submarine drainage and depression patterns. In the subducting plate area, the NW—SE trending primary normal fault system represented by trench-paralleled scarps was incipiently formed as the Nazca Plate was bent in the outer edge and further intensified as the plate approached the trench. The NE—SW trending secondary normal fault system that consists of discontinuous and smaller faults, usually intersect the primary trench-paralleled fault system. Similar to the Nazca Plate, the overriding continental plate also shows two major NW—SE and NE—SW trending fault systems represented by fault scarps or narrow elongated depressions.The submarine drainage systems represented by a series of canyon and channel courses appear to be partly controlled by the faults and exhibit a pattern similar to the onshore drainage which flows into the central region of the coastal area. Two large depressions occurring along the middle—upper slope areas of the continental margin are recognized as collapse and slump that perhaps are a major result of increased slope gradient. The subsidence of the forearc area in the southern Peru—northern Chile Continental Margin is indicated by: a) drainage systems flowing into the central region, b) the slope collapse and slumps heading to the central region, c) the deepening of the trench and inclining of the lower slope terrace to the central region, and d) submerging of the upper-slope ridge and the Peru—Chile Coast Range off the Arica Bight area.The subsidence of the forearc area in the southern Perunorthern Chile margin is probably attributed to a subduction erosion which causes wearing away and removal of the rock and sedimentary masses of the overriding plate as the Nazca Plate subducts under the South American Plate.  相似文献   

污水-海水混合过程中磷转移的动力学   总被引：5，自引：0，他引：5       下载免费PDF全文

陈松  林汝健  廖文卓 《海洋与湖沼》1993,24(1):59-64

模拟研究厦门市污水排海时污水-海水混合过程中磷含量和形态的变化,及其在海水-颗粒物界面交换的动力学过程;提供转移模式,确定转移速率,进行磷的容量校正,为厦门西海域环境容量估算和水质控制提供定量参数和理论依据。  相似文献   

Reanalysis of the Atmospheric Flux of Nutrient Elements to the Southern Yellow Sea and the East China Sea     

万小芳吴增茂  常志清 《海洋通报(英文版)》2003,5(1):45-51

Based on the recent research results on dry and wet deposition of nutrient elements and sulphate,we estimate the atmospheric flux of nutrient elements and sulphate to the southern Yellow Sea and the East China Sea in each season.The results suggest that the concentrations of nutrient elements and sulphate in aerosol and precipitation show an apparent seasonal cycle with the maximum values in winter and the minimum values in summer.Depositions of nitrate and sulphate are dominated by wet deposition,while the deposition for phosphate is mainly dry deposition.Moreover,compared with the riverine inputs,the atmospheric deposition may be the main source of dissolved inorganic nutrients in the southern Yellow Sea and the East China Sea.  相似文献