Isolation,biodegradation ability and molecular detection of hydrocarbon degrading bacteria in petroleum samples from a Brazilian offshore basin     

Suzan Pantaroto de Vasconcellos  Elaine Crespim  Georgiana Feitosa da Cruz  Diego Barbosa Senatore  Karen Christina Marques Simioni  Eugênio Vaz dos Santos Neto  Anita Jocelyne Marsaioli  Valéria Maia de Oliveira 《Organic Geochemistry》2009,40(5):574-588

The detection of microorganisms with potential for biodeterioration and biodegradation in petroleum fields is of great relevance, since these organisms may be related to a decrease in petroleum quality in the reservoirs or damage in the production facilities. In this sense, petroleum formation water and oil samples were collected from the Campos Basin, Brazil, with the aim of isolating microorganisms and evaluating their ability to degrade distinct classes of hydrocarbon biomarkers (9,10-dihydrophenanthrene, phytane, nonadecanoic acid and 5α-cholestane). Twenty eight bacterial isolates were recovered and identified by sequencing their 16S rRNA genes. Biodegradation assays revealed that bacterial metabolism of hydrocarbons occurred through reactions based on oxidation, carbon–carbon bond cleavage and generation of new bonds or by the physical incorporation of hydrocarbons into microbial cell walls. Based on the biodegradation results, selective PCR-based systems were developed for direct detection in petroleum samples of bacterial groups of interest, namely Bacillus spp., Micrococcus spp., Achromobacter xylosoxidans, Dietzia spp. and Bacillus pumilus. Primer sets targeting 16S rRNA genes were designed and their specificity was confirmed in silico (i.e. computational analysis) and in PCR reactions using DNA from reference strains as positive and negative controls. Total DNA from oil was purified and the amplification tests revealed the presence of the target bacteria in the samples, unraveling a significant potential for petroleum deterioration in the reservoirs sampled, once proper conditions are present for hydrocarbon degradation. The application of molecular methods for rapid detection of specific microorganisms in environmental samples would be valuable as a supporting tool for the evaluation of oil quality in production reservoirs.  相似文献   

A preliminary in situ Raman spectroscopic study of the oxygen isotope exchange kinetics between H₂O and (PO₄)_aq     

Thorsten Geisler  Argyrios KasioptasMartina Menneken  Christina PerdikouriAndrew Putnis 《Journal of Geochemical Exploration》2009

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Petrologic and textural diversity among the PCA 02 howardite group,one of the largest pieces of the Vestan surface     

Andrew W. BECK  Kees C. WELTEN  Harry Y. McSWEEN Jr.  Christina E. VIVIANO  Marc W. CAFFEE 《Meteoritics & planetary science》2012,47(6):947-969

Abstract— Nine howardites and two diogenites were recovered from the Pecora Escarpment Icefield (PCA) in 2002. Cosmogenic radionuclide abundances indicate that the samples are paired and that they constituted an approximately 1 m (diameter) meteoroid prior to atmospheric entry. At about 1 m in diameter, the PCA 02 HED group represents one of the largest single pre‐atmospheric pieces of the Vestan surface yet described. Mineral and textural variations were measured in six of the PCA 02 howardites to investigate meter‐scale diversity of the Vestan surface. Mineral compositions span the range of known eucrite and diogenite compositions. Additional non‐diogenitic groups of Mg‐ and Fe‐rich olivine are observed, and are interpreted to have been formed by exogenic contamination and impact melting, respectively. These howardites contain olivine‐rich impact melts that likely formed from dunite‐ and harzburgite‐rich target rocks. Containing the first recognized olivine‐rich HED impact melts, these samples provide meteoritic evidence that olivine‐rich lithologies have been exposed on the surface of Vesta. Finally, we present a new method for mapping distributions of lithologies in howardites using 8 elemental X‐ray maps. Proportions of diogenite and eucrite vary considerably among the PCA 02 howardites, suggesting they originated from a heterogeneous portion of the Vestan surface. While whole sample modes are dominated by diogenite, the finer grain size fractions are consistently more eucritic. This discrepancy has implications for near‐infrared spectral observations of portions of Vesta’s surface that are similar to the PCA 02 howardites, as the finer grained eucritic material will disproportionately dominate the spectra.  相似文献   

Bleaching of the post‐IR IRSL signal: new insights for feldspar luminescence dating     

Romée H. Kars  Tony Reimann  Christina Ankjærgaard  Jakob Wallinga 《Boreas: An International Journal of Quaternary Research》2014,43(4):780-791

Post‐infrared (pIR) stimulated luminescence dating of sedimentary feldspar largely avoids the effects of anomalous fading that affect conventional infrared stimulated luminescence (IRSL) dating. However, optical resetting of pIR signals is more difficult than resetting the conventional IRSL signal, which may undermine the crucial assumption that pIR signals were effectively bleached upon deposition and burial of sediment grains. In this study, we quantify the bleaching properties of several pIR signals on various samples using laboratory‐simulated bleaching in full sunlight and water‐attenuated sunlight. Our data show that bleaching is most efficient under full spectrum conditions for all pIR signals and that pIR signals measured at elevated temperature are increasingly harder to bleach than IR and pIR signals measured at low temperature (e.g. IR at 50°C). All bleaching curves exhibit a very slow and steady decrease, indicating that a fixed un‐bleachable residual level cannot be reached within the 11 days of solar simulator exposure undertaken here. We show that the magnitude of a laboratory‐determined residual dose depends on the adopted bleaching protocol and cannot be used as a proxy for the dose that remains in the sample at the time of burial (remnant dose). Our data emphasize the importance of finding a balance between sufficient signal stability and a minimized contribution of a remnant dose when using pIR procedures for feldspar luminescence dating.  相似文献   

Fluid flow and pore pressure development throughout the evolution of a trough mouth fan,western Barents Sea     

Jaume Llopart  Roger Urgeles  Carl Fredrik Forsberg  Angelo Camerlenghi  Maarten Vanneste  Michele Rebesco  Renata Giulia Lucchi  Denise Christina Rüther  Hendrik Lantzsch 《Basin Research》2019,31(3):487-513

Using a combination of geophysical and geotechnical data from Storfjorden Trough Mouth Fan off southern Svalbard, we investigate the hydrogeology of the continental margin and how this is affected by Quaternary glacial advances and retreats over the continental shelf. The geotechnical results show that plumites, deposited during the deglaciation, have high porosities, permeabilities and compressibilities with respect to glacigenic debris flows and tills. These results together with margin stratigraphic models obtained from seismic reflection data were used as input for numerical finite element models to understand focusing of interstitial fluids on glaciated continental margins. The modelled evolution of the Storfjorden TMF shows that tills formed on the shelf following the onset of glacial sedimentation (ca. 1.5 Ma) acted as aquitards and therefore played a significant role in decreasing the vertical fluid flow towards the sea floor and diverting it towards the slope. The model shows that high overpressure ratios (up to λ ca. 0.6) developed below the shelf edge and on the middle slope. A more detailed model for the last 220 kyrs accounting for ice loading during glacial maxima shows that the formation of these aquitards on the shelf focused fluid flow towards the most permeable plumite sediments on the slope. The less permeable glacigenic debris flows that were deposited during glacial maxima on the slope hinder fluid evacuation from plumites allowing high overpressure ratios (up to λ ca. 0.7) to develop in the shallowest plumite layers. These high overpressures likely persist to the Present and are a critical precondition for submarine slope failure.  相似文献   

Refining the ice flow chronology and subglacial dynamics across the migrating Labrador Divide of the Laurentide Ice Sheet with age constraints on deglaciation     

Jessey M. Rice  Martin Ross  Roger C. Paulen  Samuel E. Kelley  Jason P. Briner  Christina M. Neudorf  Olav B. Lian 《第四纪科学杂志》2019,34(7):519-535

The Laurentide Ice Sheet was characterized by a dynamic polythermal base. However, important data and knowledge gaps have led to contrasting reconstructions in areas such as the Labrador Ice Divide. In this study, detailed fieldwork was conducted at the southeastern edge of a major landform boundary to resolve the relative ice flow chronology and constrain the evolution of the subglacial dynamics, including the migration and collapse of the Labrador Ice Divide. Surficial mapping and analysis of 94 outcrop‐scale ice flow indicators were used to develop a relative ice flow chronology. ¹⁰Be exposure ages were used with optical ages to confine the timing of deglaciation within the study area. Four phases of ice flow were identified. Flow 1 was a northeasterly ice flow preserved under non‐erosive subglacial conditions associated with the development of an ice divide. Flow 2 was a northwest ice flow, which we correlate to the Ungava Bay Ice Stream and led to a westward migration of the ice divide, preserving Flow 2 features and resulting in Flow 3's eastward‐trending indicators. Flow 4 is limited to sparse fine striations within and around the regional uplands. The new optical ages and ¹⁰Be exposure ages add to the regional geochronology dataset, which further constrains the timing of ice margin retreat in the area to around 8.0 ka. Copyright © 2019 The Authors. Journal of Quaternary Science Published by John Wiley & Sons Ltd.  相似文献   

Another Typical Day in this Typical Town: place as inspiration for music creation and creative expression     

Christina Ballico 《The Australian geographer》2017,48(3):349-363

Geographically isolated within its own State as well as from the rest of the country, and with the highest percentage of metropolitan primacy of all Australian States, Perth is a city somewhat uncomfortable with its creative and cultural identity. The city is physically peripheral to networks in the arts and creative industries that operate at the national level. And, symbolically, many in Perth’s cultural sector feel a sense of isolation. Yet, in certain cultural sectors, such as music, Perth demonstrably punches above its weight, consistently generating high-quality cultural exports. This paper unpicks this curious juxtaposition. It examines the influence of Perth—as a geographical location and a site of creative inspiration—on the music created and disseminated by local contemporary musicians who write and perform their own indie pop/rock music. I discuss the nuanced relationship between creative clustering and place, drawing on theories regarding the relationship between music and place, the notion of the music scenes and creativity and creative practices. I explore how living in Perth, a geographically isolated city, with a highly concentrated population and associated music sector, influences creative expressions, desires and inspirations, for a select group of local indie pop/rock musicians who achieved national and international notoriety with their music in the late 1990s and 2000s. Navigating isolation but nevertheless energised by strong social connections and scene participation, Perth musicians catalysed creativity in ways that contribute to evolving debates on the links between place, creativity and music.  相似文献   

The exceptionally wet year of 2014 over Greece: a statistical and synoptical-atmospheric analysis over the region of Thessaloniki     

Konstantia?TolikaEmail author  Panagiotis?Maheras  Christina?Anagnostopoulou 《Theoretical and Applied Climatology》2018,132(3-4):809-821

The highest rainfall totals (912.2 mm) and the largest number of raindays (133 days), since 1958, were recorded in Thessaloniki during the year of 2014. Extreme precipitation heights were also observed on a seasonal, monthly and daily basis. The examined year presented the highest daily rainfall intensity, the maximum daily precipitation and the largest number of heavy precipitation days (greater than 10 mm), and it also exceeded the previous amounts of precipitation of very wet (95th percentile) and extremely wet (99th percentile) days. According to the automatic circulation type classification scheme that was used, it was found that during this exceptionally wet year, the frequency of occurrence of cyclonic types at the near surface geopotential level increases, while the same types decreased at a higher atmospheric level (500 hPa). The prevailing type was type C which is located at the centre of the study area (Greece), but several other cyclonic types changed during this year not only their frequency but also their percentage of rainfall as well as their daily precipitation intensity. It should be highlighted that these findings differentiated on the seasonal-scale analysis. Moreover, out of the three teleconnection patterns that were examined (Scandinavian Pattern, Eastern Mediterranean Teleconnection Pattern and North Sea-Caspian Pattern), the Scandinavian one (SCAND) was detected during the most of the months of 2014 meaning that it was highly associated with intense precipitation over Greece.  相似文献   

A stochastic multivariate framework for modeling movement of discrete sediment particles in open channel flows   总被引：1，自引：1，他引：0  

Jungsun?Oh  Christina?W.?TsaiEmail author 《Stochastic Environmental Research and Risk Assessment (SERRA)》2018,32(2):385-399

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Between-Population Differences in Multi-stressor Tolerance During Embryo Development in the American Horseshoe Crab, <Emphasis Type="Italic">Limulus polyphemus</Emphasis>     

M. Christina Vasquez  H. Jane Brockmann  David Julian 《Estuaries and Coasts》2017,40(5):1487-1501

The American horseshoe crab, Limulus polyphemus, is found along the Atlantic and Gulf of Mexico coasts in genetically isolated populations. Eggs are laid in shoreline beaches that expose developing embryos to combinations of environmental stressors. Whether populations of L. polyphemus differ in multi-stressor tolerance had never been tested. We assessed the multi-stressor tolerance of L. polyphemus embryos from a population in Delaware Bay (DE) and determined whether these differed from the multi-stressor tolerance of embryos from a more southerly Florida Gulf Coast (FGC) population. We monitored the field sediment temperatures and determined multi-stressor tolerance of DE embryos, then compared these to published data for FGC embryos. For multi-stressor tolerance, we assessed development success of embryos in 2-week exposures to 36 full-factorial combinations of temperature (20, 25, 30, 35 °C), salinity (5, 15, and 34 ppt), and ambient O₂ (5, 13, and 21% O₂), followed by 2 weeks in recovery conditions. Sediment temperatures in the DE site ranged from 9.5 to 46 °C, with extended periods exceeding 35 °C. Development success was similar between the DE and FGC populations in 14 of 26 multi-stressor combinations. The DE embryos were generally more successful in conditions that included high temperature or moderate hyposalinity, whereas the FGC embryos were generally more successful in conditions that included extreme hyposalinity. This suggests that although multi-stressor tolerances are generally similar between the two populations, specific differences exist that correlate more with differences in nest microenvironment than latitude.  相似文献