Stress-induced apoptosis in sea urchin embryogenesis     

Vega RL  Epel D 《Marine environmental research》2004,58(2-5):799-802

We find that early sea urchin embryos have the capability to induce programmed cell death, or apoptosis, in response to chemical and physical stress. Strongylocentrotus purpuratus embryos (fertilized, 4 cell, 16 cell, 64 cell, and early blastula) were exposed to known cytotoxins, in order to determine when apoptosis occurs naturally and in response to stress. Using cell permeability as an indicator of early stage apoptosis, caspase activation as a mid-stage indicator, and DNA fragmentation as a late stage indicator, we find that during the cleavage stage of embryogenesis apoptosis is almost completely absent. However, a statistically significant (p<0.001) rise in apoptosis in stressed embryos is evident around 24 h after fertilization, during the early blastula stage and shortly after hatching. Before this stage, exposed embryos show no statistically significant increases in apoptosis in comparison to the controls. This pattern of apoptosis in development is similar to that seen in lower vertebrate models in which stress-induced apoptosis occurs only around the mid-blastula transition. We conclude that apoptosis may be used to rid embryos of aberrant or damaged cells in early development, but this response is stage-dependant. Repair, rather than apoptosis, may be utilized during earlier stages, or alternatively, embryos exposed to such stressors may continue development with damaged cells and perhaps damaged DNA. Our continued studies will focus on these alternative hypotheses.  相似文献   

Preliminary studies On the colonial form of Cylindrotheca sp. （Bacillariophyceae）     

M.R.Li B.Z.Bian 《山东海洋学院学报》1986,16(4):215-216

From a stock culture of Cylindrotheca sp., non-motile colony-forming cells were observed and isolated.Colony-forming cells produced both single cells and colony-forming cells in liquid medla.the rate of single cell production varied among clones.  相似文献   

用纤维素乙酸平板电泳测定中国卤虫的同功酶变异(英文)     

张雷  CharlesE.king 《中国海洋大学学报(自然科学版)》1991,(4)

本文报导了应用纤维素乙酸平板电泳方法,测定采于中国山东省东方红盐场卤虫(Artemia)的同功酶。经测定,8种同功酶其中有6种是多态的,即:苹果酸脱氢酶(MDH)、异柠檬酸酶(IDH)、谷氨酸草醋酸转移酶(GOT)、葡糖-6-磷酸盐脱氢酶(D6PHD)、6-磷酸萄糖脱氢酶(6PGDH)和磷酸葡糖异构酶(PGI)。本文所提供的研究方法,不仅适用于基础研究,而且在水产养殖业上有助于对卤虫的筛选和确证。  相似文献   

第23章 DNS模式:一种为大洋波浪预报而改进的谱模式     

J.H.Auender  T.P.Barnett  M·Lybanon 《海洋预报》1989,(Z1)

23.1 介绍 模拟与频率和方向相关的大洋波谱F（f,θ）的工作是极其困难的。唯一有点统一的理论（Hasselmann,1967）也还没有简化成一种实用形式。这篇研究中所探讨的是结合零散的,现有的理论,以达到这样一种目的:即发展一种与我们目前对风浪成长谱的了解相一致 ·95.的波谱模式。本章中我们将讨论一种离散的谱模式,这种模式采用了一种改进的能量传播的特征方法。数值方法最早是由Barnett等（1969）提出的。  相似文献   

“大洋矿产国际会议——天然气水合物专题”论文摘要——１、天然气水合物在燃料矿产中的地位     

V.A.Soloviev  杨木壮 《海洋地质》2002,(3):24-26

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“大洋矿产国际会议——天然气水合物专题”论文摘要——7、厌氧条件下甲烷微生物氧化作用形成的碳酸盐构造的地球化学特征     

A.Y.Lein  杨木壮 《海洋地质》2002,(3):37-38

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大西洋扇贝贝壳生长年轮的氧同位素研究   总被引：2，自引：0，他引：2  

蔡德陵  F. C. Tan  D. L. Roddick 《海洋与湖沼》1990,21(6):550-558

两只活的大洋扇贝Placopecten magellanicus分别于1983和1985年采自加拿大诺瓦·斯科舍省布朗斯浅滩,其氧同位素记录展示出一种年度性的循环,并与由环境海水的实测温度和同位素组成所预计的贝壳同位素组成十分接近。外生长线的位置与这些循环中δ~(18)O的最大值吻合,这说明外生长线于底层海水温度最低时(春季)形成。据测定的δ~(18)O的结果证明,外生长线是年度性形成的,这与生物学证据一致。  相似文献   

Estrogenic responses of larval sunshine bass (Morone saxatilis x M. Chrysops) exposed to New York City sewage effluent     

Todorov JR  Elskus AA  Schlenk D  Ferguson PL  Brownawell BJ  McElroy AE 《Marine environmental research》2002,54(3-5):691-695

To determine the estrogenicity of effluents from sewage treatment plants (STPs) to larval fish, 2-day-old sunshine bass were exposed to effluents from three STPs serving New York City (NYC), varying in size and treatment level. Estrogenic response was evaluated by measuring vitellogenin (VTG) and estrogen receptor (ER) expression in cytosolic fractions of whole body homogenates. Concentrations of the presumptive endocrine disruptors in the effluents were also measured. VTG and ER levels in sewage-exposed fish were 3-5 times that observed in controls. Combined concentrations of estradiol and estrone ranged from 5 to 13 ng/l and nonylphenol-ethoxylate metabolites (NPEOs: 4-nonylphenol, and 1-, 2-, and 3-nonylphenol-ethoxylates) ranged from 180 to 470 microg/l in chlorinated effluent. Results indicate that both ER and VTG can be used as biomarkers for endocrine disruption in larval fish, and that 4-day exposure to sewage effluent is sufficient to elicit significant expression of these markers in sunshine bass larvae. The extremely higher concentrations of NPEOs found in effluent relative to hormones (approximately 40,000-fold) indicates that surfactant metabolites may be contributing significantly to the estrogenic effects observed.  相似文献   

远景圈闭与成藏组合的地质风险评价指南     

White.DA  大道 《海洋地质译丛》1994,(4):1-10

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澳大利亚海岸概况     

D.沃克  J.沙贝尔 《热带海洋学报》1983,(2)

澳大利亚是个大陆岛,界于南纬11°—43°,东西长5000公里,面积约770万平方公里(包括塔斯马尼亚岛等)。她与南极大陆隔以3000公里的海域。在澳大利亚和亚洲大陆之间有一列岛屿,其中新几内亚位于与澳大利亚相同的大陆架上,在第四纪时通常是相连的。  相似文献