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通过印度尼西亚苏门答腊岛Padang Cermin热泉环境基因组DNA构建古菌16S rRNA基因文库,并利用PCR-RFLP技术对其古菌多样性和系统发育分析进行了研究.根据限制性内切酶AluⅠ和MspⅠ的特征性酶切图谱,将62个阳性克隆归类为14个分类操作单位(operational taxonomic unit,OTU),文库的覆盖度达90.32％.克隆文库的优势类群为OTU2和OTU1,分别占克隆文库的27.42％和20.96％.从每个OUT中选取一个代表性克隆进行16S rRNA基因的序列测定与系统发育分析,结果表明,测定的Padang Cermin热泉中的古菌均属于泉古菌门,包括热变形菌目(Thermoproteales)、硫还原球菌目(Desulfurococcales)、杂色泉古菌(miscellaneous crenarchaeotic group,MCG)和未培养泉古菌(uncultured Crenarchaeota,UC),该热泉代表性克隆与GenBank数据库已有16S rRNA序列的相似性为91.9％～97.8％,而且与其相似性最高的序列均来自未培养的古菌克隆,由结果可见,Padang Cermin热泉古菌群落与已报道的其他热泉古菌群落的相似性较低,表明该热泉可能具有某些独特的特征,存在着特殊的古菌生态类群. 相似文献
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A. V. Frolov V. V. Asmus I. V. Zemlyanov O. I. Zil’bershtein V. A. Krovotyntsev V. A. Martyshchenko E. A. Mironov 《Russian Meteorology and Hydrology》2009,34(3):148-158
In accordance with the contract of the LUKOIL Oil Company, a cooperation of the Roshydromet organizations (Planeta Research Center for Space Hydrometeorology, a main contractor, Hydrometeorological Research Center of the Russian Federation, Arctic and Antarctic Research Institute, and State Oceanographic Institute) carried out in 2008 complex studies of the hydrometeorological and ice conditions for the Filanovskii oil- and gas-field facility construction on the northwestern shelf of the Caspian Sea. Three expeditions were organized and conducted within that project: a helicopter ice research expedition (specialists from the Arctic and Antarctic Research Institute carried out a huge volume of measurements of physicomechanic properties of level, rafted and hummocked ice, and morphometric characteristics of ice piling, hummocks, and stamukhas); specialists from the State Oceanographic Institute organized a ship expedition on studying sea ground exaration formed due to impacts of ice formations (hummocks and stamukhas) using hydro-radar and echo-sounder surveys as well as a complex hydrometeorological and hydrochemical expedition with five autonomous buoy stations mounted in two months. From the moment of ice formation to the end of the expedition activity, an operational space monitoring of the northwestern Caspian Sea was carried out at the Planeta Research Center for Space Hydrometeorology. Based on the NOAA, TERRA, and AQUA satellite data, corrected and geographically fixed satellite images of the area of activity were issued with a periodicity of 6 times per day; index maps on the ice situation (twice a week) and ice situation forecasts (lead-time of 1–7 days). Besides, long-term series of satellite data on the northwestern Caspian Sea are collected and processed: their results are used for estimating seasonal and interannual variability of the drift ice and fast ice. Specialists of the Hydrometeorological Research Center of the Russian Federation completed the work on processing and analysis of library materials, research/technical reports, handbooks, expedition observational data, and on hydrodynamic and probability modeling of long-term series of hydrological, meteorological, and partly ice data. In particular, basic characteristics of the hydrological regime (sea level, currents, and waving) are calculated for the place of the oil platform location and along the pipeline routing. Tentative local specifications on the hydrometeorological regime in the Filanovskii field are worked out based on the results of the work performed. 相似文献
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S. K. Popov O. I. Zil’bershtein A. L. Lobov M. M. Chumakov 《Russian Meteorology and Hydrology》2009,34(12):801-809
The Caspian Sea level and flow velocity are calculated with a three-dimensional baroclinic model with free surface using the
reanalysis data on meteorological variables for 1948–2004. The results are compared with the data on the sea level observations
at the coastal stations. It is shown that the model adequately reproduces intraannual changes of the sea level. For effective
computations with multiprocessor computers, a parallel model version is developed, which has a significant advantage of the
usual version in the computation speed. 相似文献
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I. Wayan Budiasa I. Gusti Ngurah Santosa I. Nyoman Sunarta I. Ketut Suada I. Nyoman Rai A. A. I. Ratna Dewi V. Dias S. Moyzhes N. Shchegolkova 《Water Resources》2018,45(1):138-147
The article offers a new approach to the selection of a treatment plant, based on the optimization of biogeochemical matter flows. The existing treatment facilities of Bali are analyzed. The authors propose several process schemes for domestic wastewater treatment, depending on the technology of utilization of biogenic elements from the wastewater for agricultural production. These are based on water treatment technologies that have been in use in Moscow for more than 100 years. 相似文献
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采用MSM寡营养培养基从印度尼西亚Pantai cermin,Kalianda和Banyu wedang地区的热泉水样、泥样以及沉积物中根据菌落的形态学特征共分离获得69株细菌,通过菌株的16S rDNA的限制性片段长度多态性(PCR-RFLP)分析、序列比对和生理生化分析,对其进行鉴定并构建系统发育树.结果表明,分离... 相似文献
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Natural hazards in Central Java Province,Indonesia: an overview 总被引：2，自引：0，他引：2
Muh Aris Marfai Lorenz King Lalan Prasad Singh Djati Mardiatno Junun Sartohadi Danang Sri Hadmoko Anggraini Dewi 《Environmental Geology》2008,56(2):335-351
Central Java Province, Indonesia, suffers from natural hazard processes such as land subsidence, coastal inundation, flood,
volcanic eruption, earthquake, tsunami, and landslide. The occurrence of each kind of natural hazard is varied according to
the intensity of geo-processes. It is necessary to learn from the historical record of coastal inundation, flood, volcanic
eruption, earthquake, tsunami, and landslide hazards in Central Java Province to address issues of comprehensive hazard mitigation
and management action. Through the understanding about the nature and spatial distribution of natural hazards, treatments
can be done to reduce the risks. This paper presents the natural hazard phenomena in Central Java Province and provides critical
information for hazard mitigation and reduction. 相似文献