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241.
The recent increase in demand and transportation security highlights the importance of the public transit system in the United States. This study explores how potential failures on nodal disruptions affect transit system flows and examines the change in the reliability of transit systems with a case study of the Greater Metropolitan Area of Washington, DC. For methodology, we employ network reliability and system flow loss and assess the criticality of stations under a variety of simulated nodal disruptions. We evaluate network resilience by identifying the best and worst geographical impact scenarios on networks.  相似文献   
242.
Carbonic anhydrase (CA), a ubiquitous enzyme found in many species, including fishes, is involved in physiological functions such as pH homeostasis, calcification, photosynthesis, and ionic regulation. CA I, a member of the α-CA family, is a cytoplasmic isozyme involved in carbon dioxide transport, ion exchange, and acid-base balance. Approximately half of the extant shark species occur only in deep waters; however, few published studies on sharks include these taxa. As fisheries worldwide enter deeper waters, the provision of biological data for these little-known taxa is critical to their management and conservation. To address this limitation, we aimed to detect CA I in various tissues of the shortspine spurdog (Squalus mitsukurii) and characterize its physicochemical properties by using sodium dodecyl-sulfate polyacrylamide gel electrophoresis and isoelectric focusing, together with immunohistochemistry. CA I was detected on SDS-PAGE and western blot analysis as a specific band at 29 kDa in various tissues of the shortspine spurdog, and as a specific band at pI 6.5 in various tissues of the shortspine spurdog by IEF and western blot analysis. CA I immunoreactivity in various tissues of the shortspine spurdog was detected in intracellular locations. To our knowledge, this is the first report of the localization of CA isozymes in various tissues of S. mitsukurii.  相似文献   
243.
Vestimentiferan tubeworms acquire their symbionts through horizontal transmission from the surrounding environment. In the present study, we constructed a 16S rRNA gene clone library to investigate the phylogenetic relationship between diverse microbes in the sediment and symbiotic bacteria in the trophosome of the tubeworm, Lamellibrachia satsuma, from Kagoshima Bay, Japan. Two symbiotic bacterial phylotypes belonging to the classes γ- and ε-Proteobacteria were found from this tubeworm trophosome. They were very closely related to the symbionts of several other marine invertebrates. The most predominant bacteria in the sediment were ε-Proteobacteria. A broad diversity of bacteria belonged to non-proteobacterial phyla such as Planctomycetes, Acidobacteria, and Chloroflexi was observed. The presence of sulfur oxidizers (i.e., ε-Proteobacteria and γ-Proteobacteria) and sulfur reducers (i.e., δ-Proteobacteria) may play a significant role in the sulfur cycle in these habitats and provide multiple sources of nutrition to the cold-seep communities. Closely related clones of ε-Proteobacteria symbiont in the species level and of γ-Proteobacteria symbiont in the genus level were found in the surrounding sediment. The similarity of symbiont clones of L. satsuma with other symbionts and free-living bacteria suggests the possibility of opportunistic symbiosis in ε-Proteobacteria and the co-evolution of γ-Proteobacteria having occurred after symbiosis with the tubeworms.  相似文献   
244.
Spatial optimization techniques are commonly used for regionalization problems, often represented as p-regions problems. Although various spatial optimization approaches have been proposed for finding exact solutions to p-regions problems, these approaches are not practical when applied to large-size problems. Alternatively, various heuristics provide effective ways to find near-optimal solutions for p-regions problem. However, most heuristic approaches are specifically designed for particular geographic settings. This paper proposes a new heuristic approach named Automated Zoning Procedure-Center Interchange (AZP-CI) to solve the p-functional regions problem (PFRP), which constructs regions by combining small areas that share common characteristics with predefined functional centers and have tight connections among themselves through spatial interaction. The AZP-CI consists of two subprocesses. First, the dissolving/splitting process enhances diversification and thereby produces an extensive exploration of the solution space. Second, the standard AZP locally improves the objective value. The AZP-CI was tested using randomly simulated datasets and two empirical datasets with different sizes. These evaluations indicate that AZP-CI outperforms two established heuristic algorithms: the AZP and simulated annealing, in terms of both solution quality and consistency of producing reliable solutions regardless of initial conditions. It is also noted that AZP-CI, as a general heuristic method, can be easily extended to other regionalization problems. Furthermore, the AZP-CI could be a more scalable algorithm to solve computational intensive spatial optimization problems when it is combined with cyberinfrastructure.  相似文献   
245.
246.
Seven species of Pleuronectidae (Platichthys stellatus, Kareius bicoloratus, Pseudopleuronectes herzensteini, Microstomus achne, Glyptocephalus stelleri, Hippoglossoides dubius, and Limanda punctatissima) were identified based on eggs collected from the western margin of the East Sea (east coast of Korea), using DNA barcoding. The eggs of P. herzensteini and G. stelleri were relatively widely distributed along the east coast of Korea, whereas those of P. stellatus and K. bicoloratus were narrowly distributed; in particular, the eggs of P. stellatus were restricted to areas near bays. The eggs, which share common morphological characteristics (such as a homogeneous yolk and smooth membrane) were divided into three groups according to egg size: group A (more than 1.60 mm in egg diameter, including genera Hippoglossoides and Microstomus), group B (1.00–1.60 mm, including genera Kareius, Glyptocephalus, and Platichthys), and group C (less than 1.00 mm, including genera Limanda and Pseudopleuronectes). This paper provides an overview of the morphological characteristics of the eggs of the family Pleuronectidae collected from the east coast of Korea. Our approach to the analysis of eggs, based on DNA barcoding, morphological characteristics, and geographic distributions, provides a rapid and accurate basis for identifying spawning areas and spawning periods, thus facilitating the assessment and management of fisheries stocks and resources.  相似文献   
247.
Korean Geostationary Ocean Color Imager (GOCI) and Moderate Resolution Imaging Spectroradiometer (MODIS) Aqua observations of the variation in ocean color at the sea surface were utilized to monitor the impact of nutrient-rich sewage sludge disposal in the oligotrophic area of the Yellow Sea. MODIS revealed that algal blooms persisted in the spring annually at the dump site in the Yellow Sea since year 2000 to the present. A number of implications of using products of the satellite ocean color imagers were exploited here based on the measurements in the Yellow Sea. GOCI observes almost every hour during the daylight period, every day since June 2011. Therefore, GOCI provides a powerful tool to monitor waste disposal at sea in real time. Tracking of disposal activity from a large tanker was possible hour by hour from the GOCI timeseries images compared to MODIS. Smaller changes in the color of the ocean surface can be easily observed, as GOCI resolves images at smaller scales in space and time in comparison to polar orbiting satellites, e.g., MODIS. GOCI may be widely used to monitor various marine activities in the sea, including waste disposal activity from ships.  相似文献   
248.
The high primary production enhanced by anticyclonic eddies and hourly variation pattern in the productivity during the spring season in the East Sea were first investigated using the first Korean Geostationary Ocean Color Imager (GOCI). Even though the stratification for a seasonal spring bloom is not well developed in the water column in early April in the East Sea, a physical upward water flux movement at the periphery of the anticyclonic eddies could remain the phytoplankton in euphotic zone to sustain high chlorophyll-a concentration conditions in the Ulleung Basin. At this time, nutrients were no major controlling factor for phytoplankton growth since concentrations of major nutrients (nitrate, silicate, and phosphate) were relatively high in the observed eddy sites based on the observation data from the Korean Oceanographic Data Center (KODC). The estimated mixed layer depth (MLD) significantly shallower at the periphery supports for this mechanism. The hourly primary productivity estimation based on a Carbon-based Productivity Model (CbPM) provides a bimodal pattern along the time especially in L1 with an approximately one order magnitude difference between the lowest and highest values of productivities on 5 April, 2011. Potential possibilities for this large discrepancy in the hourly productivity and some thoughts on a short time in situ incubation method were discussed.  相似文献   
249.
Water resources are influenced by various factors such as weather, topography, geology, and environment. Therefore, there are many difficulties in evaluating and analyzing water resources for the future under climate change. In this paper, we consider climate, land cover and water demand as the most critical factors affecting change in future water resources. We subsequently introduce the procedures and methods employed to quantitatively evaluate the influence of each factor on the change in future water resources. In order to consider the change in land cover, we apply the Multi-Regression approach from the cellular automata-Markov Chain technique using two independent variables, temperature and rainfall. In order to estimate the variation of the future runoff due to climate change, the data of the SRES A2 climate change scenario were entered in the SLURP model to simulate a total of 70 years, 2021–2090, of future runoff in the Han River basin in Korea. However, since a significant amount of uncertainties are involved in predicting the future runoff due to climate change, 50 sets of daily precipitation data from the climate change scenario were generated and used for the SLURP model to forecast 50 sets of future daily runoff. This process was used to minimize the uncertainty that may occur when the prediction process is performed. For future water balance analysis, the future water demand was divided into low demand, medium demand and high demand categories. The three water demand scenarios and the 50 daily runoff scenarios were combined to form 150 sets of input data. The monthly water balance within the Han River basin was then calculated using this data and the Korean version of Water Evaluation and Planning System model. As a result, the future volume of water scarcity of the Han River basin was predicted to increase in the long term. It is mostly due to the monthly shift in the runoff characteristic, rather than the change in runoff volume resulting from climate change.  相似文献   
250.
Seismic reliability assessment of lifeline networks gives rise to various technical challenges, which are mostly caused by a large number of network components, complex network topology, and statistical dependence between component failures. For effective risk assessment and probabilistic inference based on post‐hazard observations, various non‐simulation‐based algorithms have been developed, including the selective recursive decomposition algorithm (S‐RDA). To facilitate the application of such an algorithm to large networks, a new multi‐scale approach is developed in this paper. Using spectral clustering algorithms, a network is first divided into an adequate number of clusters such that the number of inter‐cluster links is minimized while the number of the nodes in each cluster remains reasonably large. The connectivity around the identified clusters is represented by super‐links. The reduced size of the simplified network enables the S‐RDA algorithm to perform the network risk assessment efficiently. When the simplified network is still large even after a clustering, additional levels of clustering can be introduced to have a hierarchical modeling structure. The efficiency and effectiveness of the proposed multi‐scale approach are demonstrated successfully by numerical examples of a hypothetical network, a gas transmission pipeline network, and a water transmission network. Copyright © 2014 John Wiley & Sons, Ltd.  相似文献   
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