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41.
Pyrosequencing reveals specific associations of bacterial clades Roseobacter and Flavobacterium with the harmful dinoflagellate Cochlodinium polykrikoides growing in culture 
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下载免费PDF全文 Harmful algal blooms of the dinoflagellate Cochlodinium polykrikoides are of worldwide environmental concern. Previous field studies have demonstrated the association of such algal blooms with free‐living bacteria (FLB) and particle‐associated bacteria (PAB). In the present study, we report evidence for bacterial association with C. polykrikoides in laboratory culture. Sampling was performed at different growth stages (lag, exponential and stationary) and pyrosequencing was used to identify taxa. As a result, the community structures of FLB and PAB were elucidated and significant differences between FLB and PAB were identified by hierarchical cluster analysis and the similarity profiles test (p = .05). The relative abundances of bacterial operational taxonomic units distinctly changed in response to the different phases of C. polykrikoides growth. The Roseobacter genus Marivita and the Flavobacterium genus Winogradskyella were dominant in the FLB and PAB associated with C. polykrikoides, respectively. The Roseobacter clade, which has also exhibited associations with C. polykrikoides blooms in field samples, may influence host cell growth through the provision of vitamins. 相似文献
42.
Ki Young Kim Jung Mo Lee Wooil Moon Chang-Eob Baag Heeok Jung Myung Ho Hong 《Pure and Applied Geophysics》2007,164(1):97-113
In order to investigate the velocity structure of the southern part of the Korean peninsula, seismic refraction profiles were
obtained along a 294-km WNW-ESE line and a 335-km NNW-SSE line in 2002 and 2004, respectively. Seismic waves were generated
by detonating 500–1000 kg explosives in drill holes at depths of 80–150 m. The seismic signals were recorded by portable seismometers
at nominal intervals of 1.5–1.7 km. Separate velocity tomograms were derived from first arrival times using a series expansion
method of travel-time inversion. The raypaths indicate several mid-crust interfaces including those at approximate depths
of 2–3, 15–17, and 22 km. The Moho discontinuity with refraction velocity of 7.8 to 8.4 km/s has a maximum depth of 37–39
km under the southern central portion of the peninsula. The Moho becomes shallower as the Yellow Sea and the East Sea are
approached on the west and east coasts of the peninsula, respectively. The depth of the 7.6 km/s velocity contour varies from
29.4 km to 36.5 km. The discrepancy in depth between the seismological Moho and the interpreted critically refracting interface
may result from the presence of a gradual transition between the crust and mantle. The velocity tomograms show particular
crustal structures including (1) the existence of an over 70-km wide low-velocity zone centered at 6–7 km depth under the
Okchon fold belt and Ryeongnam massif, (2) existence of high-velocity materials under the Gyeongsang basin, and (3) the downward
extension of the Yeongdong fault to depths greater than 10 km. 相似文献
43.
Characteristics and sources of inertia-gravity waves revealed in the KEOP-2007 radiosonde data 总被引：1，自引：0，他引：1
Characteristics and sources of inertia-gravity waves are investigated using high-resolution radiosonde data observed at ten stations in Korea during 15 June to 15 July 2007. The wave analyses are performed in the lower stratospheric region (Z = 17–30 km). The average intrinsic frequency, vertical wavelength, and horizontal wavelength for the observed waves are 2.77f (where f is the Coriolis parameter), 2.58 km, and 620.11 km, respectively. The average eastward and westward momentum fluxes are 0.005 m2 s?2 and ?0.003 m2 s?2, respectively, and the average northward and southward momentum fluxes are 0.007 m2 s?2 and ?0.002 m2 s?2, respectively. To understand the propagation and the sources of the observed gravity waves, a three-dimensional ray-tracing model is used. The observed gravity waves are classified into two groups based on the existence of convection when and where the rays reach altitudes of 6–13 km. Sources are mostly located in the northeast and southeast of the observation stations below Z = 5 km for the convection-related cases (CONV), while those for the other cases (NCONV) are located in the northeast and southeast of the observation stations above Z = 20 km. The average intrinsic frequency and vertical wavelength of the CONV cases are somewhat larger than those of the NCONV cases. The average potential, kinetic, and total wave energies of the CONV cases are less than those of the NCONV cases. 相似文献
44.
In this paper, first we introduce the wave run-up scale which describes the degree of wave run-up based on observed sea conditions near and on a coastal structure. Then, we introduce a simple method which can be used for daily forecast of wave run-up on a coastal structure. The method derives a multiple linear regression equation between wave run-up scale and offshore wind and wave parameters using long-term photographical observation of wave run-up and offshore wave forecasting model results. The derived regression equation then can be used for forecasting the run-up scale using the offshore wave forecasting model results. To test the implementation of the method, wave run-up scales were observed at four breakwaters in the East Coast of Korea for 9 consecutive months in 2008. The data for the first 6 months were used to derive multiple linear regression equations, which were then validated using the run-up scale data for the remaining 3 months and the corresponding offshore wave forecasting model results. A comparison with an engineering formula for wave run-up is also made. It is found that this method can be used for daily forecast and warning of wave run-up on a coastal structure with reasonable accuracy. 相似文献
45.
Fabien Lombard Thomas Kiørboe 《Deep Sea Research Part I: Oceanographic Research Papers》2010,57(10):1304-1313
The evolution of size, sinking velocity, and dry weight of aging discarded appendicularian houses, a component of marine snow, were examined in laboratory experiments. The sizes of discarded houses decrease over time, with a rapid deflation during the first hour, followed by a slower rate of compression leading to a total of 60% and 87% decrease in diameter after 1 h and 5 d, respectively. The initial rapid deflation of the houses is accompanied by a massive loss of its particle content and a 10–63% loss in weight. The initial weight loss is left as a trail of elevated particle and solute concentration in the wake of the sinking house. Subsequently the house weight decreases at a much lower rate that is consistent with bacterial degradation. The combined effect of weight losses and deflation–compression process is an increase in the sinking speed of the houses, by a factor of 1.7–6 after 1.5–3 d. These processes can provide a new insight on the sinking dynamic and flux of appendicularian produced marine snow from in situ observations. We applied our laboratory derived rates to field data from the East Atlantic Ocean and estimate that large (2000–4000 μm) houses account for about 1/3 of the 300–500 μm particles in the upper 100 m and loose 30% of their mass before leaving the upper 200 m. The observed deflation–compression process may have several consequences on the dynamics of appendicularian-derived marine snow particles. First, it may explain field observations that marine snow sinking velocities increase with depth. Second, an initial rapid loss of weight and particles will decrease the potential vertical flux of particulate carbon due to appendicularians. And finally, the trail of particles and solutes may guide zooplankton to the sinking house, and further increase its degradation due to grazing by detrivorous organisms. 相似文献
46.
Effects of humic acid on heavy metal uptake by herbaceous plants in soils simultaneously contaminated by petroleum hydrocarbons 总被引：2，自引：0，他引：2
Soyoung Park Ki Seob Kim Daesok Kang Hansam Yoon Kijune Sung 《Environmental Earth Sciences》2013,68(8):2375-2384
The effects of humic acid (HA) on heavy metal uptake by herbaceous plants in soil simultaneously contaminated with heavy metals and petroleum hydrocarbons were investigated. The results showed that HA reduced readily soluble and exchangeable forms of heavy metals in the contaminated soil but increased their plant-available forms. Potential bioavailability and leachability factors became larger than 1 after adding HA to the soil, except for those of Ni, suggesting that more heavy metals could be potentially phytoavailable for plant uptake. Furthermore, HA increased the accumulation of Pb, Cu, Cd, and Ni in the shoots and roots of selected plants. The greatest increase in the accumulation of heavy metals was 264.7 % in the shoot of Festuca arundinacea, with the bioconcentration factor (BCF) increasing from 0.30 to 1.10. Humic acid also increased the BCFs of the roots of Brassica campestris for Ni and Pb. These results suggest that HA amendment could enhance plant uptake of heavy metals, while concurrently reducing heavy metal leachability and preventing subsurface contamination, even in soils simultaneously contaminated with petroleum hydrocarbons. 相似文献
47.
Sung-Suk Suh Mirye Park Jinik Hwang Sukchan Lee Sang Hyun Moh Ki Hwan Park Taek-Kyun Lee 《Ocean Science Journal》2014,49(3):193-200
Understanding the patterns of microbial diversity and their composition in seawater is necessary to assess the impacts of microbes on marine ecosystem. Although the potential roles of microbes in the ocean have been studied, their diversity and relationship with environmental factors remain unclear. In this study, we adopted a 16S rRNA gene tag-pyrosequencing technique to investigate the bacterial communities associated with two different water masses from Tongyoung in the South Sea of Korea. Our results revealed highly diverse bacterial communities up to 900 Operational Taxonomic Units (OTUs) estimated from each seawater which was collected in the month of March and May, when the environmental conditions including temperature differed significantly: 7.2°C and 17.6°C in March and May, respectively. Altogether, 13 bacterial phyla were recovered from the seawater, of which Proteobacteria was the most dominant group. In addition, the value of the Shannon index, which measures the evenness of the distribution of individuals among OTUs, in May is higher than that in March, indicating that it displays a wider diversity of bacteria. Interestingly, the proportion of pathogenic bacteria was significantly increased in the month of May compared to March, suggesting that pathogenic bacteria were increasingly emerging in May. In particular, Pseudoalteromonas and Vibrio spp. were determined as major pathogenic bacteria from both water masses, of which Vibrio spp. were dominant. 相似文献
48.
Yong-Sang Choi Heeje Cho Chang-Hoi Ho Richard S. Lindzen Seon Ki Park Xing Yu 《Theoretical and Applied Climatology》2014,115(1-2):355-364
Recent studies have estimated the magnitude of climate feedback based on the correlation between time variations in outgoing radiation flux and sea surface temperature (SST). This study investigates the influence of the natural non-feedback variation (noise) of the flux occurring independently of SST on the determination of climate feedback. The observed global monthly radiation flux is used from the Clouds and the Earth's Radiant Energy System (CERES) for the period 2000–2008. In the observations, the time lag correlation of radiation and SST shows a distorted curve with low statistical significance for shortwave radiation while a significant maximum at zero lag for longwave radiation over the tropics. This observational feature is explained by simulations with an idealized energy balance model where we see that the non-feedback variation plays the most significant role in distorting the curve in the lagged correlation graph, thus obscuring the exact value of climate feedback. We also demonstrate that the climate feedback from the tropical longwave radiation in the CERES data is not significantly affected by the noise. We further estimate the standard deviation of radiative forcings (mainly from the noise) relative to that of the non-radiative forcings, i.e., the noise level from the observations and atmosphere–ocean coupled climate model simulations in the framework of the simple model. The estimated noise levels in both CERES (>13 %) and climate models (11–28 %) are found to be far above the critical level (~5 %) that begins to misrepresent climate feedback. 相似文献
49.
Ying?WuEmail author Jing?Zhang Ki?Woong?Cho Gi?Hoon?Hong Chang?Soo?Chung 《Estuaries and Coasts》2004,27(4):583-592
The biogeochemistry of organic matter (OM) in a macrotidal estuary, the Yalujiang River, was studied during two cruises: the
flood season in August 1994 and the dry season in April 1996. Surface sediments were collected in the riverine zone (RZ),
the turbidity maximum zone (TMZ), and the marine zone (MZ). The molecular distribution of the n-alkanes and fatty acid series
and bulk sediment characteristics, such as C:N and δ13C, were used to assess differences in OM source and transport from the river upstream to the marine end member. Higher C:N
values typical for terrestrial sources were observed at the upper reach for both seasons. The δ13C of OM in surface sediments varied from −27.3‰ to −21.6‰ in the flood season and from −26.8‰ to −31‰ in the dry season. The
concentrations of n-alkanes varied between 0.3–21.4 μg g−1 and the variation of fatty acids was 4.8–32.9 μg g−1. The data showed mixing of terrestrial and autochthonous OM in the middle and lower reaches. The distribution of lipids (n-alkanes
and Carbon Preference Index) encountered in this study confirmed the importance of terrestrial OM in the sediment samples
from degraded soil material. The distribution of fatty acids suggested important phytoplankton, zooplankton, and microbial
signals (short-chain and unsaturated acids; ≤C20). Branched fatty acids, such as the iso- and anteiso-C15 and C17 compounds, relfect bacterial contributions. All samples were characterized by a high proportion of mixture inputs in both
seasons. A slight decreasing trend was observed with increasing salinity except for the highest percentage of mixed fatty
acids in the TMZ of the flood season. Terrestrial fatty acids were approximately 20% in the flood season and 27–46% in the
dry season. Differences in hydrological conditions and primary production between the TMZ, RZ, and MZ resulted in different
OM distributions, which are reflected in the sources and degree of diagenesis of the sedimentary OM. Seasonal variation may
be strongly influenced by hydrological characteristics rather than primary productivity and anthropogenic activities in the
Yalujiang region. 相似文献
50.
Electromagnetic fields in a non-uniform steel-cased borehole 总被引：1，自引：0，他引：1
Since most oil wells are cased in steel, electromagnetic (EM) signals undergo severe attenuation as they diffuse across the casing. This paper examines an effect of non‐uniform casing properties on EM fields measured in a steel‐cased well embedded in a layered formation. We use a finite‐element method for computing secondary azimuthal electric fields in a cylindrically symmetric model, and analytically obtain primary fields for a homogeneous casing in a homogeneous whole space. Although steel casing largely masks EM signals induced into a layered formation, the induced signal is more pronounced in phase than in amplitude. The effect of casing non‐uniformity is quite large in measured fields but is highly localized. When electrical conductivity varies rapidly in the casing wall, the resulting EM fields also vary rapidly. A cross‐correlation function of these variations has strong peaks at two points, the interval between them being equal to the source–receiver distance. The high‐frequency coherent noise event caused by the non‐uniform casing can be greatly suppressed by low‐pass filtering to enhance EM signals indicating formation conductivity. 相似文献