Bacterial killing by Mytilus hemocyte monolayers as a model for investigating the signaling pathways involved in mussel immune defence     

Canesi L  Scarpato A  Betti M  Ciacci C  Pruzzo C  Gallo G 《Marine environmental research》2002,54(3-5):547-551

The signaling pathways involved in mussel immune defence were investigated utilizing a model of killing of Escherichia coli by Mytilus galloprovincialis hemocytes in a co-culture setting. In particular, the role played by different mitogen activated protein kinases (MAPKs) and by the production of eicosanoids were investigated utilising specific cell permeant, pharmacological enzyme inhibitors. Hemocyte pretreatment with the p38 MAPK inhibitor SB203580 significantly reduced bacterial killing, whereas PD98059 (an inhibitor of ERK--extracellularly regulated kinase--MAPK activation) had no significant effect. Wortmannin also inhibited bacterial killing, indicating a crucial role for PI3-kinase activation in the immune response. Killing of E. coli was also reduced by inhibitors of both PLA2 and cyclooxygenase activities, indicating that eicosanoid production is involved in mediating the response to bacterial challenge. The results demonstrate that bacterial killing by mussel hemocytes is particularly sensitive to inhibitors of the key steps involved in the transduction of bacterial signals into the host cell. Moreover, these data indicate that the hemocyte bactericidal activity can be suitably utilized not only for identifying the signaling pathways involved in the response to bacterial infection, but also as a potential investigative-toxicology model to test drugs and contaminants for their effect on the overall mussel immune defence.  相似文献   

新钻探技术的试验     

И.А.Басов  朱佛宏 《海洋地质前沿》2002,18(5):21-22

在实现深海钻探计划和随后的大洋钻探计划的漫长岁月里 ,钻探技术在不断地改进。由于研制出了新的不同的设备和工艺 ,我们成功地获得了连续的沉积建造剖面 ,使得能够极为详细和可靠地恢复沉积盖层的形成史、海洋环流的变化、气候的波动和生物的演化史。但是 ,至今尚未彻底克服在基岩中钻孔所遇到的困难。当在硬质岩石出露的洋底表面区域进行钻探时 ,困难特别大 ,例如在洋中脊的轴部。稳定钻孔 ,特别是重返该钻孔的问题也没有解决。当在若干航次中采用一般工艺进行钻探时 ,确实有顺利钻入基底足够深度的例子 (例如太平洋中的 50 4B孔和印度…  相似文献   

大尺度海岸行为     

JeffreyH.List尹延鸿  张光威 《海洋地质前沿》1995,(12):11-11

大尺度海岸行为ＪｅｆｆｒｅｙＨ．Ｌｉｓｔ等该题目是指对海岸和近岸沉积系统的了解，以及对发生在该系统中的变化的预测。Ｌｉｓｔ（１９９３）定义大尺度海岸行为是几十年的时间尺度内、几至几十公里空间范围内的海岸物理演化．Ｔｅｒｗｉｎｄｔ和Ｗｉｊｎｂｅｒｇ（１...  相似文献   

地中海西北部深海水道：巴伦西亚水道的地貌,地震构造及其环境     

Alonso  B 顾兆峰 《海洋地质前沿》1996,(8):13-15

地中海西北部深海水道：巴伦西亚水道的地貌、地震构造及其环境Ｂ．Ａｌｏｎｓｏ等巴伦西亚水道位于地中海西北部巴伦西亚海槽的轴部，是一个ＮＥ—ＳＷ向构造凹陷，全长４００ｋｍ。根据海底地貌沉积特征，可把巴伦西亚水道大体划分为上、中、下三段。上段特征为：（１）...  相似文献   

海洋浮游生物调查方法的标准化     

В.Г.波戈罗夫  李冠国 《海洋与湖沼》1957

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4-tert-octylphenol and 17beta-estradiol applied by feeding to flounder Platichthys flesus: induction of vitellogenin and accumulation in tissues   总被引：1，自引：0，他引：1  

Madsen LL  Korsgaard B  Bjerregaard P 《Marine environmental research》2002,54(3-5):729-733

In order to simulate a possible natural administration route of xenoestrogens male flounder Platichthys flesus were exposed via the diet to the alkylphenol 4-tert-octylphenol. Treatment with 4-tert-octylphenol (10, 50 and 100 mg OP kg(-1) BW) or 17beta-estradiol (0.05 mg kg(-1) BW) every second day during a period of 11 days resulted in a significant increase in plasma vitellogenin (Vtg) concentrations. The induction of Vtg was greatest in the fish receiving 50 mg OP kg-' BW. A significant accumulation of 4-tert-octylphenol was found in liver and muscle tissue of the OP treated groups. The tissue concentrations of 4-tert-octylphenol and the plasma vitellogenin concentration were positively correlated. The results show that 4-tert-octylphenol accumulates in liver and muscles of flounder P. flesus, and exerts estrogenic effects such as vitellogenin induction.  相似文献   

Histopathological biomarkers in estuarine fish species for the assessment of biological effects of contaminants   总被引：1，自引：0，他引：1  

Stentiford GD  Longshaw M  Lyons BP  Jones G  Green M  Feist SW 《Marine environmental research》2003,55(2):137-159

The increasing emphasis on the assessment and monitoring of estuarine ecosystems has highlighted the need to deploy appropriate biological indices for these locations. Fish diseases and histopathology, with a broad range of causes, are increasingly being used as indicators of environmental stress since they provide a definite biological end-point of historical exposure. This study reports on the histopathological alterations observed in selected organs and tissues of three species of estuarine fish (Platichthys flesus, Pomatoschistus minutus and Zoarces viviparus), captured from four British estuaries (the Tyne, Tees, Mersey and Alde), differently impacted by contaminants, including PAHs. A biannual sampling regime was used to identify the important seasonal variations that occur in terms of the observed biological effects. Inflammatory lesions and hepatocellular fibrillar inclusions attained their highest prevalence in P. flesus captured from the Tyne, Tees and Mersey. The presence of pre-neoplastic and neoplastic toxicopathic lesions was highest in P. flesus captured from these sites, when compared to fish from the Aide reference site. In particular, the prevalence of hepatic foci of cellular alteration (up to 43.3%) and hepatocellular adenoma (up to 10%) were highest in P. flesus captured from the Mersey estuary. Intersex (ovotestis) was only recorded in male P.flesus captured from the Mersey estuary (up to 8.3%) and from male Z. viviparous captured from the Tyne estuary (25%). Pathologies associated with the gill and the kidney were also most prevalent in fish captured from the Tyne, Tees and Mersey estuaries. This study has successfully applied histopathology to an estuarine monitoring program, both for the recording of toxicopathic lesions in the liver and other organs, and for the detection of the endpoint of endocrine disruption, intersex. As such, it provides a powerful integrative tool for the assessment of biological effects of contaminants in these environments.  相似文献   

太平洋西南部铁锰矿化的矿物学特征     

А.Н.Калягкн朱佛宏  石学法 《海洋地质前沿》1995,(9):14-16

太平洋西南部铁锰矿化的矿物学特征Ａ．Ｈ．Ｋａｎ等在对克马德克和新赫布里底岛弧—海沟构造系不同地貌构造的地质采样过程中（１９９０年科学考察船“ＡＨｅｃＭＨｏＢ院士”号第１７航次），在一系列测站上采集到不同的铁锰形成物（ＭＯ）。详细研究这些形成物具有特殊...  相似文献   

五种植物提取液的抗鱼病菌和抗鱼病毒效应(英文)   总被引：1，自引：0，他引：1  

童裳亮  F.M.Hetrick  B.S.Roberson  A.Baya 《中国海洋大学学报(自然科学版)》1990,(2)

本文旨在探索用廉价的野生植物防治鱼病。所用的植物有牛繁缕(Stellaria aqu-atica),双花凤仙(Impatiens biflora),夜来香(Oenothera biennis),艾草(Artem-isia vulgaris)和忍冬(Lonicera japonica)。用作试验的鱼病菌共13种(18个菌株),分属于气单孢菌属、假单孢菌属、爱德华氏菌属、耶尔森氏菌属、弧菌属。所用的鱼病毒为传染性胰脏坏死病毒IPNV,传染性造血组织坏死病毒IHNV。上述病原微生物均获自美国马里兰大学微生物系。实验结果表明,五种植物提取液均有不同程度的抗鱼病菌作用,其中以牛繁缕的抗菌谱最广,作用力最强。忍冬、艾草、牛繁缕有抗鱼病毒作用。其中以忍冬的效果最好,它能抑制IPNV和IHNV。而艾草和牛繁缕只能抑制IHNV。  相似文献   

Improved form of wind wave frequency spectrum   总被引：5，自引：0，他引：5  

Wen Shengchang 《海洋学报(英文版)》1989,8(4):467-483

The lower frequency part of the theoretical wind wave spectrum proposed by the authors (Wen et al. , 1988a, b,c) has been improved and the form of spectrum is appreciably simplified. In addition to the field data collected in the Bohai Sea region and used in the previous papers, those obtained in the Huanghai Sea, the East China Sea and the South China Sea have been employed so that the improved spectra can be verified on a more extensive observational basis. Computed results agree with the observations well. Further comparisons have been made between the proposed spectra and the JONSWAP spectrum. Though the two types of spectrum are close to each other in form, the former shows, as a whole, better agreement with the observation than the latter. By introducing an improved relation between the peak-ness factor and significant wave steepness, the spectrum contains only significant wave height and period as parameters. For spectra given in this form, the computed peak frequencies coincide approximately wit  相似文献