Temporal Change of Dissolved Inorganic Carbon in the Subsurface Water at Station KNOT (44°N, 155°E) in the Western North Pacific Subpolar Region     

Masahide?WakitaEmail author  Shuichi?Watanabe  Yutaka?W.?Watanabe  Tsuneo?Ono  Nobuo?Tsurushima  Shizuo?Tsunogai 《Journal of Oceanography》2005,61(1):129-139

The dissolved inorganic carbon (DIC) and related chemical species have been measured from 1992 to 2001 at Station KNOT (44°N, 155°E) in the western North Pacific subpolar region. DIC (1.3∼2.3 µ mol/kg/yr) and apparent oxygen utilization (AOU, 0.7∼1.8 µmol/kg/yr) have increased while total alkalinity remained constant in the intermediate water (26.9∼27.3σ_θ). The increases of DIC in the upper intermediate water (26.9∼27.1σ_θ) were higher than those in the lower one (27.2∼ 27.3σ_θ). The temporal change of DIC would be controlled by the increase of anthropogenic CO₂, the decomposition of organic matter and the non-anthropogenic CO₂ absorbed at the region of intermediate water formation. We estimated the increase of anthropogenic CO₂ to be only 0.5∼0.7 µmol/kg/yr under equilibrium with the atmospheric CO₂ content. The effect of decomposition was estimated to be 0.8 ± 0.7 µmol/kg/yr from AOU increase. The remainder of non-anthropogenic CO₂ had increased by 0.6 ± 1.1 µmol/kg/yr. We suggest that the non-anthropogenic CO₂ increase is controlled by the accumulation of CO₂ liberated back to atmosphere at the region of intermediate water formation due to the decrease of difference between DIC in the winter mixed layer and DIC under equilibrium with the atmospheric CO₂ content, and the reduction of diapycnal vertical water exchange between mixed layer and pycnocline waters. In future, more accurate and longer time series data will be required to confirm our results.  相似文献   

Temporal Trends in Apparent Oxygen Utilization in the Upper Pycnocline of the North Pacific: 1980–2000     

Steven Emerson  Yutaka W. Watanabe  Tsuneo Ono  Sabine Mecking 《Journal of Oceanography》2004,60(1):139-147

We present a compilation of apparent oxygen utilization (AOU) changes observed in the upper pycnocline of the North Pacific Ocean over the last several decades. The goal here is to place previously-published data in a common format, and assess the causes of the observed changes. The general trend along repeat cross sections of the eastern and western subtropical ocean and the subarctic ocean is an increase in AOU from the mid 1980s to the mid 1990s. AOU has also been increasing in a time-series study in the northwest subarctic Ocean off of Japan since the late 1960s. Observed AOU changes south of 35°N in the subtropical ocean are 10–20 μmol kg⁻¹, with much greater changes, reaching 60–80 μmol kg⁻¹ in isolated areas, in the subtropical/subarctic boundary and the subarctic ocean. Analysis of changes in both AOU and salinity on isopycnals suggests that there are significant salinity-normalized increases that must be due to alteration in the rate of ventilation or organic matter degradation. A common feature in the data is that the maximum increase in AOU is centered near the density horizon σ_θ= 26.6. Time series results from the Oyashio Current region near the winter outcrop area of this density horizon indicate that surface waters there have become fresher with time, which may mean this density surface has ceased to outcrop in the latter decades of the 20th century. Whether this is due to natural decadal-scale changes or anthropogenic influences can be decided by determining future trends in AOU on these density surfaces. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Alteration of gene expression profiles in the brain of Japanese medaka (Oryzias latipes) exposed to KC-400 or PCB126     

Kei Nakayama  Naomi Sei  Yuji Oshima  Kosuke Tashiro  Yohei Shimasaki  Tsuneo Honjo 《Marine pollution bulletin》2008,57(6-12):460

Polychlorinated biphenyls (PCBs) are known as neurotoxic chemicals and possibly alter animal behavior. We previously reported that PCB-exposure induced abnormal schooling behavior in Japanese medaka (Oryzias latipes). This abnormal behavior might be caused by the functional alteration of central or terminal nervous system. To understand the mechanism(s) of behavioral change by PCB-exposure, we analyzed the gene expression profiles in the brain of medaka exposed to 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) or a PCB mixture (Kanechlor-400: KC-400) using a cDNA microarray that we constructed. Twelve FLF-II strain medaka (six individuals per treatment) were dietary exposed to PCB126 (0.01 μg/g b.w./day) or KC-400 (1 μg/g b.w./day) for three weeks. For the control, six fish were fed a control diet. After the exposure period, fish were dissected, and the brain samples were collected. The samples from control fish were pooled and used as a common reference in the microarray experiment. Microarray data were normalized by the LOWESS method, and we screened the genes whose expression levels were altered more than 1.5-fold. Gene expression profiling showed 97 down-regulated and 379 up-regulated genes in the brain of medaka exposed to PCB126. KC-400 exposure suppressed 15 genes and induced 266 genes in medaka brain. Among these genes, the expression levels of 7 and 188 genes were commonly down- or up-regulated, respectively in both treatment groups. On the other hand, 31 gene expressions were significantly different between PCB126 and KC-400 treatment groups, and three out of 31 genes were received opposite effects. In addition, the microarray data showed that thyroid hormone-responsive genes were up-regulated by PCB-exposure, which may imply that PCBs or their metabolites mimic thyroid hormone effects in the brain of PCB-exposed medaka.  相似文献   

Alteration of gene expression profiles in the brain of Japanese medaka (Oryzias latipes) exposed to KC-400 or PCB126     

Nakayama K  Sei N  Oshima Y  Tashiro K  Shimasaki Y  Honjo T 《Marine pollution bulletin》2008,57(6-12):460-466

Polychlorinated biphenyls (PCBs) are known as neurotoxic chemicals and possibly alter animal behavior. We previously reported that PCB-exposure induced abnormal schooling behavior in Japanese medaka (Oryzias latipes). This abnormal behavior might be caused by the functional alteration of central or terminal nervous system. To understand the mechanism(s) of behavioral change by PCB-exposure, we analyzed the gene expression profiles in the brain of medaka exposed to 3,3',4,4',5-pentachlorobiphenyl (PCB126) or a PCB mixture (Kanechlor-400: KC-400) using a cDNA microarray that we constructed. Twelve FLF-II strain medaka (six individuals per treatment) were dietary exposed to PCB126 (0.01 microg/g b.w./day) or KC-400 (1 microg/g b.w./day) for three weeks. For the control, six fish were fed a control diet. After the exposure period, fish were dissected, and the brain samples were collected. The samples from control fish were pooled and used as a common reference in the microarray experiment. Microarray data were normalized by the LOWESS method, and we screened the genes whose expression levels were altered more than 1.5-fold. Gene expression profiling showed 97 down-regulated and 379 up-regulated genes in the brain of medaka exposed to PCB126. KC-400 exposure suppressed 15 genes and induced 266 genes in medaka brain. Among these genes, the expression levels of 7 and 188 genes were commonly down- or up-regulated, respectively in both treatment groups. On the other hand, 31 gene expressions were significantly different between PCB126 and KC-400 treatment groups, and three out of 31 genes were received opposite effects. In addition, the microarray data showed that thyroid hormone-responsive genes were up-regulated by PCB-exposure, which may imply that PCBs or their metabolites mimic thyroid hormone effects in the brain of PCB-exposed medaka.  相似文献   

A New Automatic Subsurface Gas Monitoring System for Seismogeochemical Studies, Installed in Haruno Borehole, Shizuoka Prefecture, Central Japan     

Kazuya Miyakawa  Ruka Takama  Iwao Kawabe  Shinnichi Kariya  Tsuneo Yamauchi 《Pure and Applied Geophysics》2010,167(12):1561-1575

The results of subsurface gas monitoring by application of gas chromatography (GC) to the gas composition of bubbles associated with groundwater for seismogeochemical studies are reported. An automated gas monitoring system was used to determine gas compositions in a 500-m borehole at the Haruno Crustal Movement Observation Site (HOS), central Japan during period 1, from December 1999 to December 2000. The average ± two standard deviation (2SD) compositions of gases in this period were He = 82 ± 29 ppmV, H₂ = 170 ± 62 ppmV, Ar = 0.05 ± 0.07%, N₂ = 50 ± 8%, and CH₄ = 45 ± 6%. A new automated gas monitoring system equipped with a micro-GC was installed in the borehole at the HOS, and gas bubbles from the borehole were monitored during period 2, from December 2006 to March 2007. The average ± two standard deviation (2SD) compositions of gases in this period were He = 8 ± 7 ppmV, H₂ = 13 ± 15 ppmV, Ar = 0.6 ± 0.3%, N₂ = 66 ± 7%, and CH₄ = 14 ± 14%. The gas concentration ratios (He/Ar, H₂/Ar, N₂/Ar, and CH₄/Ar) fluctuated significantly over time and repeatedly showed abrupt spike-like increases during period 2. The gas compositions obtained in period 1 and 2 were markedly different. Over the period from 2006 to 2007, the gas bubbles were depleted in He, H₂, and CH₄ of deep origin, but enriched in Ar and N₂ of atmospheric origin. This difference can be interpreted as being due to an irreversible change of the aquifer/gas system. The present deep component in the HOS gas is estimated to have composition He = 63 ppmV, H₂ = 37 ppmV, Ar = 0.17%, N₂ = 63%, and CH₄ = 37%. The new monitoring system is able to analyze the gas composition using a smaller volume of sample gas and with greater precision than the previous system. During the 3-month monitoring period 2, the separation capacity of the capillary column of the micro-GC was sufficiently maintained to determine gas-chromatographic peak areas for the five gaseous species examined. This study confirms that the new monitoring system with micro-GC is promising for continuous subsurface gas monitoring for earthquake prediction studies.  相似文献   

Appearance pattern of tooth germs with developmental process inMylopharyngodon piceus     

Yue Pei-qi  Nakajima Tsuneo 《中国海洋湖沼学报》1995,13(2):155-161

The pharyngeal dental formula ofMylopharyngodon piceus is 4–5 as a rule, and the dentition is asymmetrical. It is difficult to identify each tooth in the larval dentition. In this paper the appearance pattern of tooth germ with development process in this fish is described in detail. The formation pattern of the left dentition is contrasted with that of the right one. In the developmental process, the left pharyngeal dentition lacks teeth at position An3. Thus the left dentition is D-type as designated by Nakajima (1984), while the right one is A-type. This project was funded by the International Cooperation of Japan-China and the National Natural Science Foundation of China.  相似文献   

Specific affinity for phosphate uptake and specific alkaline phosphatase activity as diagnostic tools for detecting phosphorus-limited phytoplankton and bacteria     

Tsuneo Tanaka  Peter Henriksen  Risto Lignell  Kalle Olli  Jukka Seppälä  Timo Tamminen  T. Frede Thingstad 《Estuaries and Coasts》2006,29(6):1226-1241

We analyzed responses of soluble reactive phosphorus (SRP), bioavailable phosphate (PO₄), particulate phosphorus, turnover time of orthophosphate (T_t), and alkaline phosphatase activity (APA) to varying degrees of nutrient stress. The nutrient stress was evoked by different treatments in concentration and combination of inorganic nitrogen (N) and phosphorus (P), and labile organic carbon (glucose) to mesocosms in experiments carried out in eutrophic southern (Odense Fjord, Denmark) and northern (Tvärminne Archipelago, Finland) coastal zones of the Baltic Sea. Despite seasonal and geographical differences, similar responses were observed in both experiments. Low SRP (<100 nmol l^?1), shortT _t (<10 h), and increased levels of APA were observed in both N+P balanced and P deficient treatments, while the opposite trend was observed in P replete treatments. The shortestT _t and the highest APA were found when glucose was combined with N treatment. Bioavailable PO₄ was estimated usingT _t and P uptake rates as derived from stoichiometric conversion of carbon based primary and bacterial production. With shorterT _t, the PO₄ pool declined to <1 nmol-P l^?1, whereas the SRP background pool (difference between SRP and PO₄) remained relatively constant (c. 50 nmol l^?1). APA was inversely related to PO₄ but not to SRP. Responses of specific APA and specific affinity for PO₄ uptake, which are APA and PO₄ uptake rates (inverse ofT _t), respectively, normalized to the summed P biomass of phytoplankton and bacteria, responded consistently to the pattern and magnitude of nutrient limitation evoked in our experiments. Our results, together with a literature survey, suggest that both parameters can be useful in examining PO₄ availability for the natural phytoplankton and bacteria community in P starved aquatic systems.  相似文献   

Estimation of the apertures of water-saturated fractures by nuclear magnetic resonance well logging   总被引：1，自引：0，他引：1  

Yoshito Nakashima  Tsuneo Kikuchi 《Geophysical Prospecting》2007,55(2):235-254

Fracture aperture is an important transport property in subsurface hydrology because it influences well productivity and the volume of the water resource. Nuclear magnetic resonance (NMR) well logging measures the hydrogen‐bearing fluid molecules in porous or fractured strata, and the NMR signal intensity increases with the amount of fluid in the sensed region of the NMR sonde. Fluid confined in a large fracture of >>0.2 mm in aperture has T2 (i.e. spin‐spin relaxation time) values as long as those of the bulk fluid. The bulk‐fluid porosity (i.e. porosity calculated using this long T2 component in a T2 histogram data) increases linearly with aperture. Therefore, NMR logging enables quantitative estimation of fracture apertures of >>0.2 mm using the bulk‐fluid porosity data if the calibration of the NMR sonde is performed adequately. We applied NMR logging to a borehole in a Holocene andesite lava at Sumikawa, Japan, to estimate the aperture of open fractures within the lava. A test well of 100 m depth and 20 cm diameter, filled with bentonite drilling mud, was scanned with an NMR sonde to obtain a profile of the porosity and the T2 histogram of the andesite. The bulk‐fluid porosity was calculated from the T2 histogram data, as the porosity at which the T2 value is larger than or equal to a threshold T2 of bulk bentonite mud. The bulk‐fluid porosity of a specific inclined fracture responsible for the total loss of circulation at 61.2 m depth during drilling was calculated assuming a threshold or T2 cut‐off of 33 ms, and again for a cut‐off of 100 ms. Calibration of the NMR sensor in a laboratory and measurement of the fracture dip angle by electrical microimaging logging enabled us to estimate the fracture aperture as 1.7 cm, assuming a T2 cut‐off of 33 ms, or 1.6 cm for a T2 cut‐off of 100 ms. The method of aperture determination described in this study is independent of fluid species and lithology, and is applicable to various hydrogen‐bearing borehole fluids (clean water, mud and oil) and geological settings.  相似文献   

Microcrystalline sphalerite in resin globules suspended in Lake Kivu,East Africa   总被引：1，自引：0，他引：1  

E. T. Degens  H. Okada  S. Honjo  J. C. Hathaway 《Mineralium Deposita》1972,7(1):1-12

The origin and chemical nature of micron-sized spheres found as suspended particles in Lake Kivu are examined. It can be shown that the hollow spheres, with a wall thickness of 500 Å, consist of a complex polymeric resinous material which has little functionality, except for hydroxyl groups. The spheres arise in the process of degassing of water samples at depth. Tiny gas bubbles, about 1 micron in size, act as scavengers of dissolved resinous material. The newly created resinous membrane promotes the selective coordination of zinc dissolved in the water column. In the prevailing H₂S regime, formation of sphalerite crystals in induced. The size range of the crystals, 5 to 50 Å, corresponds to 1 to 10 unit cells and suggests that the resinous membrane also acts as a template in sphalerite growth processes. The sources of the zinc and dissolved gases (CO₂, CH₄, H₂S) are hydrothermal springs seeping from the lake bottom into the basin. Water discharge is substantial; about 100 years are required to fill the lake to its present level (ca. 550 km³ water). The average Kivu water contains 2 ppm zinc. Thus, 1 million tons of zinc are contained in Lake Kivu in the form of sphalerite.

---

Zusammenfassung Harzkügelchen von etwa 1 Durchmesser treten in suspendierter Form im Kivusee auf. Die Kügelchen sind hohl und besitzen eine äußere Membrane von rund 500 Å Dicke. Sie sind das Ergebnis von Entgasungsvorgängen die sich in einer Wassertiefe von mehreren hundert Metern abgespielt haben. Die Oberfläche kleiner Gasblasen diente als Kristallisationszentrum für die im Wasser gelösten Harze, die ihrerseits eine selektive Koordinierung von gelöstem Zink bewirkten. Die Anwesenheit von H₂S führte zur Bildung von Zinkblende mit Kristallgrößen zwischen 5 und 50 Å. Gase (CO₂, CH₄, H₂S) und Zink entstammen salinaren hydrothermalen Lösungen, die im Gefolge vulkanischer Tätigkeit dem Seeboden auch heute noch entweichen. Der Durchschnittsgehalt von Zink im Kivusee beträgt 2 ppm, was mehr als einer Million Tonnen an Zink für den Gesamtsee entspricht.

  相似文献   

In-situ dissolution experiment of radiolaria in the central North Pacific ocean     

Jonathan Erez  Kozo Takahashi  Susumu Honjo 《Earth and Planetary Science Letters》1982,59(2):245-254

Monospecific phaeodarian radiolarian assemblages of Castanidium longispinum were suspended in plastic cages built with 225 μm nylon mesh at different water depths from 378 to 5582 m in the central North Pacific. Weight losses of these samples after a suspension period of 61 days were used to determine dissolution rates. The highest weight losses were observed at 378 m where samples lost ～90% of their initial weight. Through the main thermocline weight losses decreased from 90 to 60% and reached a constant value of 40% below it. These weight losses are roughly an order of magnitude higher than those reported by earlier workers. The higher weight losses can be attributed in part to the more soluble nature of the phaeodarian radiolarian skeletons and in part to the improved experimental technique. Kinetic considerations show that temperature is the major factor that controls silica dissolution rates in the ocean. Using an Arrehnius plot for the apparent rate constants, it can be shown that in surface water dissolution rats should be two orders of magnitude higher than in deep water below the main thermocline.  相似文献