Zinc concentration effect at the organismal,cellular and subcellular levels in the eastern oyster     

Meiller JC  Bradley BP 《Marine environmental research》2002,54(3-5):401-404

The purpose of this research was to demonstrate a concentration effect of zinc exposure at organismal, cellular and sub-cellular levels in the eastern oyster and to find associated protein expression signatures (PES) for each concentration of zinc. Oysters were exposed to six concentrations of zinc for 48 h in a controlled environment. At the organismal level, fecal material was observed as a measure of physiological health during metal exposures. At the cellular level, lysosomal destabilization was measured using hemolymph. This cellular response was significant only at the highest concentration, when the fecal index was lowest. Protein responses were monitored in the oyster following exposure to zinc. Gill tissue was excised and homogenized, and then analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and digital image analysis. Protein expression signatures were found to be specific to each concentration. The protein responses were linked to the other biological parameters measured, each of which followed a concentration gradient of zinc.  相似文献   

Variability of the O2−H2S zone structure in the Black Sea: ‘Dramatic’ or mesoscale?     

A. A. Bezborodov  V. N. Eremeev 《Physical Oceanography》1992,3(1):61-66

This paper discusses the conclusion derived during a joint U.S—Turkey expedition on the R/VKrorr on the dramatic variations of the O₂–H₂S zone in the Black Sea observed over the last 20 years. This conclusion is shown to be invalid since it was based on a comparison of data obtained at two stations only (in 1969 and 1988) that was made in various regions and in different seasons. Examples are provided for the inter-seasonal and mesoscale variations of hydrophysical and hydrochemical characteristics in the O₂–H₂S zone which, as to their scales, are comparable to or even exceed the variations registered during the expedition ofKrorr.Translated by Mikhail M. Trufanov.  相似文献   

The effect of copper pollution on mitochondrial degeneration     

E. Aloj Totaro  F.A. Pisanti  P. Glees  A. Continillo 《Marine environmental research》1986,18(4)

The authors describe the effect of copper pollution on the mitochondria from various areas of the Torpedo marmorata central nervous system. A high percentage of swollen mitochondria has been observed in neurons from animals experimentally exposed to high level of copper (4 ppm). The correlation with age pigment granules is discussed.  相似文献   

Serum biochemical and cellular responses to experimental cupric ion challenge in mussels     

G.V. Pickwell  S.A. Steinert 《Marine environmental research》1984,14(1-4)

Our intent has been to develop rapid and sensitive bioassay procedures for laboratory and field applications based on hemolymph factors in animals such as the bay mussel, Mytilus edulis. We found that mussel hemolymph could be easily and repeatedly sampled from individual animals in sufficient amounts to allow analyses for lysozyme, proteolytic activity, total protein and hemocyte counts. In static tests there seemed to be graded responses indicating release of increasing amounts of the above factors into the hemolymph in roughly direct proportion to Cu²⁺ concentration at lethal and sublethal levels. We interpreted this to be evidence for increased lysosomal and cellular disruption as cupric ion exposure was increased or prolonged. At the same time, Cu²⁺ concentrations in the mussel hemolymph increased up to an order of magnitude above ambient in the range of 26 to 267 ppb. We felt that this was evidence for the release of Cu-binding proteins into the mussel hemolymph. Simultaneously with the above events the serum granulocytes increased in numbers above control values by factors of 3 or 4 in response to copper challenge while macrophage (terminology of Moore & Lowe, 1977) counts, including controls, declined precipitously.  相似文献   

Mean current velocity measurement from a movable base     

N. P. Bulgakov  V. I. Remchukov  A. G. Evgushchenko 《Physical Oceanography》1994,5(5):395-399

This paper discusses the possibility of measurement of the current velocity vector characteristics averaged over the time of continuous regular down-up probings from a moving carrier. The measurements can be carried out either from a moving vessel or from a helicopter, floating on or hovering above the sea surface. As an illustration for the suggested technique,in situ observations compiled by a free-component (±XYZ) VEGA-3 probe in the Guiana current region in March 1990 are provided. Comparison of the results with buoy data shows good agreement within the accuracy limits of the instruments employed.Translated by Vladimir A. Puchkin.  相似文献   

Fine structure of the temperature field and the transfrontal exchange in the East Kamchatka current     

I. A. Zhabin  V. P. Tapinov  G. I. Yurasov 《Physical Oceanography》1993,4(1):81-84

CTD data are used to study the relationship between the temperature field fine structure and the location of the frontal zones and synoptic eddies in the region adjacent to the eastern coast of the Kamchatka peninsula. High levels of fine structure activity were observed in the frontal zone of the East Kamchatka current and in the peripheries of anticyclonic eddies. Estimates of the coefficient of the horizontal turbulent exchange and the transfrontal (lateral) heat flux are derived.Translated by Mikhail M. Trufanov.  相似文献   

A shipborne magnetic survey in Hudson Bay     

G. V. Haines  R. L. Coles 《Marine Geophysical Researches》1982,5(3):273-300

A marine magnetic total-field survey of Hudson Bay was conducted from 1975 to 1978. Approximately 62 000 line-km of data, or 35 000 five-minute averages, were acquired. The data were corrected for secular variation by means of a polynomial in latitude, longitude, and time, first fitted to annual mean differences from nine observatories and then integrated over time. The data were corrected for diurnal and transient variations by means of a linear interpolation, over geomagnetic latitude, of running hourly mean values from two neighbouring magnetic stations (numbering seven in all). Crossover differences are analysed in terms of a mixture of normal distributions resulting from the intermittent transient variations, and indicate the effectiveness of the corrections. The variance of the data is reduced, as a result of the corrections, by 87% during quiet periods and by 53% during disturbed periods, the weighted variance being reduced by 66%. Navigational accuracy is estimated, from a novel method utilizing crossover differences and field gradients, to be approximately 300 m. Anomalies are presented in profile form, and discussed briefly.Contribution from the Earth Physics Branch No. 973.  相似文献   

Remote determination of momentum-flux profiles in the lower atmospheric boundary layer     

R. D. Kouznetsov  V. F. Kramar  M. A. Kallistratova 《Izvestiya Atmospheric and Oceanic Physics》2006,42(6):696-703

An acoustic locator—a sodar—is a unique instrument for getting the objective characteristics of the current state of the atmospheric boundary layer (ABL) owing to a combination of such properties as remoteness, mobility, resolution, and information content. This study demonstrates the capabilities of a sodar to obtain data on the second moments of the field of wind-velocity fluctuations, in particular, the profiles of momentum flux in the lower ABL, which are very important in practical applications. A corresponding method is described, and the results of its experimental verification and some examples of its application under the conditions of different ABL stratification are presented.  相似文献   

Nitrate assimilation by zooxanthellae maintained in laboratory culture     

F. P. Wilkerson  R. K. Trench 《Marine Chemistry》1985,16(4):385-393

The ability of cultured zooxanthellae to use ammonium and nitrate was tested. The zooxanthellae were initially isolated from the anemone Aiptasia pulchella. The zooxanthellae were able to grow on media supplemented with either form of dissolved inorganic nitrogen (DIN) although ammonium was inhibitory above 300 μM. In accord with the intact symbiosis and freshly isolated algae, the cultured zooxanthellae took up ammonium. In contrast, the cultured algae demonstrated nitrate uptake and utilization. This was enhanced with DIN deprivation. Nitrate reductase was found in the algae and appeared to be inducible. Within the animal host, excretory ammonium may repress this induction.  相似文献   

On the collapse of a vertical packet of mixed patches. Laboratory and field studies     

A. M. Kudin  A. N. Lozhkin  A. V. Kholoptsev 《Physical Oceanography》1992,3(5):343-348

Visualization of a collapsing vertical packet of patches in a laboratory experiment and the results of acoustical sounding under field conditions allowed us to discriminate the stage of its evolution which was previously unknown. It is defined by the variations of the horizontal dimensions of patches and their effective scattering surface with a period ofT=150–250/N, whereN is the Brunt-Väisälä frequency. The horizontal dimensions of patches can reach 40% of their final dimensions at the evolution stage discriminated.Translated by Mikhail M. Trufanov.  相似文献