深海细菌Pseudomonas sp. IOFA1中甲醛歧化酶FM11的纯化及性质研究          下载免费PDF全文

余翔  陈兴麟  金敏  曾润颖 《应用海洋学学报》2016,35(3):418-425

从印度洋深海沉积物中分离出一株能够高效降解甲醛的假单胞菌(Pseudomonas sp.IOFA1),该菌株可在40 min内完全降解100 mg/cm~3的甲醛.通过阴离子交换柱层析对菌株破碎液中的粗酶液进行纯化,得到一条具有甲醛降解活性的单一蛋白条带.经MALDI-TOF/TOF质谱鉴定,该蛋白为甲醛歧化酶蛋白(FM11).序列分析表明FM11与其它物种已知醛脱氢酶的氨基酸序列最高相似性为65%;结构域分析表明FM11含有2个保守的锌结合位点和一个NAD结合位点.酶学性质分析结果显示,FM11的最适反应温度为40℃,在25~50℃范围内能够保留95%以上的酶活力,尤其是在高于70℃的高温环境下仍保有约50%的活力,表现出广泛的温度适应性.FM11具有非常广泛的最适反应pH值范围(pH值为5~9),且在pH值4和pH值10时仍分别保留50%和80%左右的酶活力,表现出广泛的pH适应性.在pH值为5~7的条件下孵育1 h后,FM11仍保有97%以上的酶活,且在pH值为7~10条件下孵育1 h后,仍保留90%以上的酶活,表现出极好的酸性和碱性稳定性.Ca~(2+)、Mg~(2+)在低浓度时(0.5 mmol/dm~3)对FM11有较明显的激活作用.这些研究为我们进一步开发Pseudomonas sp.IOFA1甲醛歧化酶在降解甲醛方面的用途奠定了基础.  相似文献   

Re–Os geochronology of the Cambrian stage-2 and -3 boundary in Zhijin County,Guizhou Province,China     

Shuaichao Wei  Yong Fu  Houpeng Liang  Zhihua Ge  Wenxi Zhou  Guangzhe Wang 《中国地球化学学报》2018,37(2):323-333

The black shale series that formed in the Ediacaran–Cambrian transition are important stratigraphic records of the co-evolution of the paleo-ocean, -climate, and -biology. In this study, we measured Re–Os isotopic compositions of the black shale in the Niutitang Formation from the Gezhongwu section in Zhijin, Guizhou Province. The samples had high Re and Os contents, with Re ranging from 21.27 to 312.78 ng/g and Os ranging from 0.455 to 7.789 ng/g. The Re–Os isotope isochron age of 522.9 ± 8.6 Ma implies deposition of the Niutitang black shale predated the Chengjiang Fauna, providing an age constraint for the expansion of oceanic anoxia in the study area. The initial ¹⁸⁷Os/¹⁸⁸Os ratio of 0.826 ± 0.026 indicates that enhanced continental weathering might have triggered the expansion of the oceanic anoxia.  相似文献   

Petrogenesis and Tectonic Significance of the Three‐Period Porphyries from the Daruoluolong Cu (Au) Deposit,Tibet, China     

GAO Ke  SONG Yang  LIU Zhibo  FANG Xiang  LI Faqiao  LI Haifeng 《《地质学报》英文版》2018,92(3):1267-1269

正Objective The Daruoluolong deposit is the first high-grade Cu(Au)deposit discovered in the middle section of the Bangonghu-Nujiang(herein after referred to as Ban-Nu)metallogenic belt,which has not been documented about previously.This deposit is located in Shuanghu County of northern Tibet,and its geotectonic position belongs to the  相似文献   

Residual-state creep of clastic soil in a reactivated slow-moving landslide in the Three Gorges Reservoir Region,China     

Shun Wang  Wei Wu  Jinge Wang  Zhenyu Yin  Deshan Cui  Wei Xiang 《Landslides》2018,15(12):2413-2422

We study the creep properties of clastic soil in residual state. The intact samples are taken from a reactivated slow-moving landslide in the Three Gorges Reservoir Region, China. Firstly, the patterns of the landslide movement are analysed based on recent monitoring data, which indicate that the soil within the shear zone is undergoing two deformation processes: a creep phase, characterised by different creep rates, and a dormant phase. We then study the creep behaviour of the soil samples through a series of ring shear creep tests under various shear stress conditions. The creep response depends strongly on the ratio of the shear stress to the residual strength, and the normal effective stress, whereas the creep rate decreases due to strength regain. The long-term strength of the clastic soil is close to the residual strength. Therefore, the residual strength obtained from conventional shear test, which is less time consuming than creep test, can be used in long-term stability analyses of creeping landslides.  相似文献   

Numerical simulation of lifetime and time-dependent damage for granite by continuous and discontinuous approaches     

Wei Chen  Xiang Li  Heinz Konietzky 《Environmental Earth Sciences》2018,77(16):594

In this study, the time-dependent damage process of granite is investigated utilizing two numerical simulation schemes based on continuous method and discontinuous method. Numerical creep tests are carried out with both simulation schemes and mechanical responses and fracture patterns of rock specimens are analyzed. The calibrated numerical models can successfully reflect the typical creep stages observed in the laboratory. The predicted lifetime is in accordance with the laboratory test data. Comparisons are made between the two simulation schemes. It is found that both schemes have unique features that can promote a genuine reflection of the time-dependent damage process of the brittle rocks.  相似文献   

Carbonate formation mechanism in paleosol sediments of the Bohai Sea coastal zone,China     

Shuhuan Du  Baosheng Li  Rong Xiang  Dongfeng Niu  Yuejun Si 《Arabian Journal of Geosciences》2018,11(19):584

The Bohai Sea coastal zone of China consists of the Pacific Ocean to the east and Eurasia to the west; hence, this region is influenced by both the ocean and continental landmasses. The carbonate formation mechanism of eolian sediment within this area is poorly understood. The loess-paleosol sediments of the Miaodao stratigraphical section (MDS) contain a record of carbonate changes in this region during the last interglacial period. New insights into regional carbonate formation mechanisms since the last interglacial period were obtained by analyzing the ages of various sedimentary facies in combination with proxy paleoclimatic indices (including average grain size, standard deviation, CaCO₃ content, and clay minerals), as well as via foraminiferal analysis. The results led to three principal findings: (1) The carbonate content change in the MDS was neither controlled by grain size nor affected by minerals. The carbonate change controlled by precipitate leaching in the Loess Plateau region cannot explain the eolian sediments within the Bohai Sea coastal zone. (2) Various subsections contain obvious carbonate content changes caused by foraminiferal deposits atop the eolian sediments, which were deposited by storm surges during a high sea-level period. This increased the carbonate content in the eolian sediments and restricted the carbonate content in the Bohai Sea coastal zone. (3) Newly detected foraminifera of the last interglacial period on the eolian sediment of the MDS were the main source of carbonate content, providing a new understanding of the carbonate formation mechanism in eolian sediments different from that of the Loess Plateau.  相似文献   

蚌埠隆起老山片麻状二长花岗岩的成岩与变质特征及其地质意义   总被引：1，自引：0，他引：1  

陈洁  汪相  王耀 《地质论评》2018,64(5):1141-1166

蚌埠隆起是华北地块上中生代地质研究的一个十分难得的地质单元,它映现了中国东部最重要的两次构造运动(印支运动和燕山运动)所引起的岩浆作用和变质作用,其中,老山片麻状二长花岗岩就是一个代表性产物。该花岗岩的岩相学、地球化学以及锆石学的系统研究显示,它具有两个不同的岩石学特征:①它的斜长石斑晶的牌号(An_(14～18))、相对低硅高钙的主量元素、富Ba、Sr和贫Rb及无Eu负异常的微量元素等特征,以及它所含的岩浆锆石({211}锥面发育、较强的CL亮度及韵律环带构造、包含残核、正常的Hf含量)的U-Pb年龄(223.1 Ma)和ε_(Hf)(t)值(平均值为-14.2),表明它应该是印支运动的同造山花岗岩;②它的片麻状构造(斑晶矿物压扁拉长、各种交代—熔蚀结构)和变质结晶锆石({101}锥面发育、较弱的CL亮度、较高的Hf含量)的U-Pb年龄(156.2 Ma)和ε_(Hf)(t)值(平均值为-17.8),说明它在燕山运动中遭受了同造山变质作用。因此,老山片麻状二长花岗岩的存在,一方面使得大别造山带作为印支期陆陆碰撞造山带的岩石学记录完整,同时也制约了华南与华北两大板块之间碰撞挤压作用峰期的准确时间;另一方面,对燕山运动的起始时间(约170 Ma左右)和挤压高峰出现的时间(155±3 Ma)作出了合理的推断。这两方面的研究结果对于深入了解中国东部中生代的构造演化、岩石类型、成矿作用的现象及其机制具有深远的意义。  相似文献   

Two Phases of Mineralization in the Dabaoshan Polymetallic Deposit,Guangdong Province: Constraints from Re-Os Geochronology of Black Carbonaceous Mudstone and MolybdeniteEI北大核心CSCD     

Xiang J.  Liang X.  Shan Y.  Wang C.  Dong C.  Yu S.  Tan Z. 《大地构造与成矿学》2018,(4):732-745

The Dabaoshan polymetallic deposit is a polygenetic composite ore deposit located at south of the Qinhang (Qinzhou-Hangzhou) Metallogenic Belt, which is composed mainly of SEDEX type Cu-Pb-Zn and porphyry-skarn type W-Mo orebodies. Systematic field study shows that the W-Mo mineralization exhibits as quartz-vein type and skarn type orebodies around the granodioritic porphyry, or superimposes on the SEDEX type Cu-Pb-Zn orebody, while the Cu-Pb-Zn mineralization occurs mainly in the Qiziqiao Formation as stratiform-substratiform type and breccia type orebodies with typical SEDEX-type characteristics. Re-Os dating of five molybdenite separates from porphyry-skarn type W-Mo orebodies yielded isochron ages of 163.6±1.0 Ma (MSWD=0.58), which represents the age of W-Mo mineralization. Re-Os dating of seven black carbonaceous mudstone samples from a Cu-Pb-Zn orebody yielded isochron age of 387.6±9.9 Ma (MSWD=56), which is interpreted to be the age of SEDEX-type Cu-Pb-Zn mineralization. These ages indicate that there are at least two stages of ore mineralization in the Dabaoshan polymetallic deposit. The W-Mo mineralization is related to the Yanshanian intermediated-acidic intrusions, while the stratiform-substratiform SEDEX-type Cu-Pb-Zn mineralization is associated with the Hercynian seafloor hydrothermal activities. © 2018, Science Press. All right reserved.  相似文献   

Spatio-temporal Distribution of Three Kinds of Flash Droughts over Agricultural Land in China from 1983 to 2015   总被引：1，自引：0，他引：1  

Xiang Zhang  Nengcheng Chen  Chuli Hu  Xiaoting Peng 《地球科学进展》2018,33(10):1048-1057

At present, flash drought occurs globally and regionally and causes a lot of socio-economic loss in a very short time. Therefore, flash drought has been regarded as one of the hottest issues in drought research. However, flash drought monitoring, prediction and decision-making have encountered a lot of challenges due to its multiple driven factors and complex spatio-temporal process. Aiming at this problem, this paper focused on the agricultural land in China, and analyzed the spatio-temporal distribution of three kinds of flash droughts (i.e., precipitation-deficit, high-temperature, and composite flash droughts) from 1983 to 2015. We studied the occurrences, duration, spatial distribution, temporal distribution, and trend of all three kinds of flash droughts. Our results demonstrated that, the occurrences of flash drought agricultural land in China increased year by year, among which high-temperature flash drought increased dramatically; duration of flash droughts had different trends, but the variations were relatively smooth; Northeast China was identified as a vulnerable area of flash drought, indicating more flash drought events and longer duration; flash droughts in China were found to concentrate in spring (high-temperature drought) and summer seasons (precipitation-deficit drought). This study is helpful for building new flash drought monitoring method and system, and it is also valuable for flash drought preparedness on regional scale.  相似文献   

新时代地热行业发展特点及几点思考     

李宁波  李翔  杨俊伟  于湲 《城市地质》2018,(2):18-24

当前,中国特色社会主义进入了新时代,社会各项建设取得重大成就,地热产业也发展壮大。在大力倡导生态文明建设、推进绿色发展、提升环境保护、推进资源的节约和循环利用的社会发展形势下,一些重要规划和政策的实施、一批重点区域和重大项目的建设、大量地热能开发利用新技术的推广,又给地热产业带来了新的发展契机。不同形式、不同利用方式的地热能都将迎来更广阔的市场、更高的产业增长态势。为顺应新时代行业发展,地热产业应抓住机遇,迎接挑战,创新突破,成就未来。本文将从上位决策、项目建设、技术创新、管理等方面,分析当下地热行业发展的形势、特点和存在的问题,提出相关建议,以共同探讨,相互借鉴。  相似文献