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31.
Kei Nakayama Naomi Sei Yuji Oshima Kosuke Tashiro Yohei Shimasaki Tsuneo Honjo 《Marine pollution bulletin》2008,57(6-12):460
Polychlorinated biphenyls (PCBs) are known as neurotoxic chemicals and possibly alter animal behavior. We previously reported that PCB-exposure induced abnormal schooling behavior in Japanese medaka (Oryzias latipes). This abnormal behavior might be caused by the functional alteration of central or terminal nervous system. To understand the mechanism(s) of behavioral change by PCB-exposure, we analyzed the gene expression profiles in the brain of medaka exposed to 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) or a PCB mixture (Kanechlor-400: KC-400) using a cDNA microarray that we constructed. Twelve FLF-II strain medaka (six individuals per treatment) were dietary exposed to PCB126 (0.01 μg/g b.w./day) or KC-400 (1 μg/g b.w./day) for three weeks. For the control, six fish were fed a control diet. After the exposure period, fish were dissected, and the brain samples were collected. The samples from control fish were pooled and used as a common reference in the microarray experiment. Microarray data were normalized by the LOWESS method, and we screened the genes whose expression levels were altered more than 1.5-fold. Gene expression profiling showed 97 down-regulated and 379 up-regulated genes in the brain of medaka exposed to PCB126. KC-400 exposure suppressed 15 genes and induced 266 genes in medaka brain. Among these genes, the expression levels of 7 and 188 genes were commonly down- or up-regulated, respectively in both treatment groups. On the other hand, 31 gene expressions were significantly different between PCB126 and KC-400 treatment groups, and three out of 31 genes were received opposite effects. In addition, the microarray data showed that thyroid hormone-responsive genes were up-regulated by PCB-exposure, which may imply that PCBs or their metabolites mimic thyroid hormone effects in the brain of PCB-exposed medaka. 相似文献
32.
Nakayama K Sei N Oshima Y Tashiro K Shimasaki Y Honjo T 《Marine pollution bulletin》2008,57(6-12):460-466
Polychlorinated biphenyls (PCBs) are known as neurotoxic chemicals and possibly alter animal behavior. We previously reported that PCB-exposure induced abnormal schooling behavior in Japanese medaka (Oryzias latipes). This abnormal behavior might be caused by the functional alteration of central or terminal nervous system. To understand the mechanism(s) of behavioral change by PCB-exposure, we analyzed the gene expression profiles in the brain of medaka exposed to 3,3',4,4',5-pentachlorobiphenyl (PCB126) or a PCB mixture (Kanechlor-400: KC-400) using a cDNA microarray that we constructed. Twelve FLF-II strain medaka (six individuals per treatment) were dietary exposed to PCB126 (0.01 microg/g b.w./day) or KC-400 (1 microg/g b.w./day) for three weeks. For the control, six fish were fed a control diet. After the exposure period, fish were dissected, and the brain samples were collected. The samples from control fish were pooled and used as a common reference in the microarray experiment. Microarray data were normalized by the LOWESS method, and we screened the genes whose expression levels were altered more than 1.5-fold. Gene expression profiling showed 97 down-regulated and 379 up-regulated genes in the brain of medaka exposed to PCB126. KC-400 exposure suppressed 15 genes and induced 266 genes in medaka brain. Among these genes, the expression levels of 7 and 188 genes were commonly down- or up-regulated, respectively in both treatment groups. On the other hand, 31 gene expressions were significantly different between PCB126 and KC-400 treatment groups, and three out of 31 genes were received opposite effects. In addition, the microarray data showed that thyroid hormone-responsive genes were up-regulated by PCB-exposure, which may imply that PCBs or their metabolites mimic thyroid hormone effects in the brain of PCB-exposed medaka. 相似文献
33.
A seismic assessment of two multi-tier pagodas by numerical analysis is presented herein.The Changu Narayan temple and the Kumbeshwar temple in Nepal are used as the case studies.Both pagodas are built of brick masonry in earthen mortar,with timber columns and crossbeams.The Changu Narayan temple is a two-tier pagoda,and was seriously damaged during the 2015 Gorkha earthquake.The Kumbeshwar temple is a five-tier pagoda,and its top-tier collapsed due to the Gorkha earthquake.A seismic assessment was carried out using finite element(FE)analysis.The FE models were prepared,and dynamic identification tests and penetrometer tests were conducted.Pushover analysis and nonlinear dynamic analysis were performed as part of the seismic assessment.The main shock of the 2015 Gorkha earthquake was considered as the input accelerograms.The behavior between the two pagodas was compared with the collapse mechanisms and damage patterns observed in the actual structures.The comparison suggested common structural features of multi-tier pagodas.This study is dedicated to providing a better understanding of the seismic behavior of multi-tier pagoda-type structures and provides suggestions for their effective analysis. 相似文献