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51.
The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis. 相似文献
52.
Cheng Yinhe Zhou Shengqi Wang Dongxiao Lu Yuanzheng Huang Ke Yao Jinglong You Xiaobao 《中国海洋湖沼学报》2016,34(3):619-628
The observed characteristics of lower atmospheric ducts over the South China Sea(SCS) were analyzed based on Global Position Systerm(GPS) radiosonde data collected four times daily during autumn open cruises from 2006 to 2012.Duct occurrence,thickness,and strength over the SCS were about 40%,150-m thick,and 8 M units,respectively,which were larger than during the summer monsoon period.Most ducts occurred at heights 1 500 m and these ducts easily trap electromagnetic wave clusters with wavelengths 2 m.Diurnal variation of the SCS ducts appeared evident.They occurred more often at midnight at higher altitudes(about 1 100 m),with a thickest layer of about 145 m and less frequently during the evening at lower altitudes(about 800 m),with a thinnest layer of about 125 m.Moreover,ducts during the daytime at a mean height of about 900 m,with the greatest strength of about 10 M units.Furthermore,all duct variables observed over the SCS in autumn decreased from north to south.These findings are useful not only in the design of radar and communication systems,but also for evaluating possible effects of anomalous propagation on meteorological radar and military applications. 相似文献
53.
The mean velocity of debris flow is one of the most important parameters in the design of mitigation structures and in quantitative risk analysis. This study develops a model to predict the mean debris flow velocity observed in the field by applying the incomplete similarity argument. An equation for estimating the Darcy-Weisbach resistance coefficient for debris flows with a volumetric sediment concentration larger than 0.19 is accordingly derived using 128 sets of observation data from nine Chinese gullies, in which both the effect of the volumetric sediment concentration and channel slope on resistance are considered. The derived equation is then verified and compared against five previously published equations by using 61 sets of published observation data from six gullies located in four countries. The applications of the proposed equation are discussed, and the improvements made using the proposed equation are clearly very significant when compared with the previously published equations. 相似文献
54.
Cracks appeared on the northern batter at Maddingley Brown Coal Open Pit Mine, Victoria, Australia, on 8 November 2013 and a 2-day rainfall event happened 5 days later. This study models the stability of the northern batter considering the effect of the rainfall event and an emergency buttress using finite element method (FEM) encoded in Plaxis 3D. It is found that the batter tended to lead to block sliding after overburden removal. The observed vertical crack would be a combined action of the overburden removal and groundwater flow. The simulated location of cracks agrees well with the actual location, and the simulated heave of the coal seam is in good agreement with the experience in Victoria brown coal open pit mining. The rainfall accelerated the development of the cracks. With the construction of the emergency buttress, the batter became stable that is in good agreement with the monitored data. 相似文献
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为建立一个较严密、系统的常见变质岩石的岩相学分类,采用既反映岩石结构构造特征又蕴含岩石基本组成的变质岩组构组分作为岩石分类的一级分类指标,以岩石结构、构造和组构组分的成分依次作为二、三和四级指标,划分了归属于造山区域变质岩、接触热变质岩、断层动力变质岩、蚀变交代变质岩和混合岩这5个大类变质成因的基本岩石.如岩石的地质产状已知,可用对应于地质产状的成因类型变质岩替换组构组分,升级为一级分类指标,然后用同样程序进行划分.本分类方案中,造山区域变质岩与接触热变质岩因具有相同组构组分,因此,大部分基本岩石名称相同,但可借助其地质产状和一些特征变质矿物的特殊显微构造的有无将它们区分开来.本分类方案的分类效果优于国内外现有的岩相学分类方案. 相似文献
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氟是煤中有害元素之一,燃煤型氟排放造成的污染已经对人类的健康安全构成威胁。研究煤中氟赋存状态及其沉积环境,对掌握高氟煤分布规律及控制燃煤造成的氟排放具有重要意义。通过对沁水煤田内6座煤矿15号煤样品的分析发现:煤中氟含量与灰分显著相关,与黄铁矿硫、有机硫无相关关系,表明煤中氟主要以无机物形式赋存,且与含硫矿物无关;与钾、镁、硅、铝等元素含量具有显著的相关关系,表明氟有可能的主要赋存形式为金云母、氟金云母等矿物,以及以吸附存在于高岭石、勃姆石、伊蒙混层等黏土矿物中;与钙、磷元素含量无显著相关性,表明氟不以氟磷灰石、含氟羟基磷灰石等矿物大量赋存。此外,通过对沁水煤田15号煤的沉积环境分析发现,弱还原环境、较低盐度、偏酸性、较强的水动力条件有利于该区域煤中氟的富集。 相似文献
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为探讨内蒙古呼包鄂地区大气对污染物承载能力的变化,科学评价气候变化对大气环境的影响,利用1961—2016年呼包鄂地区8个国家气象站降水量、风速、云量资料,分析了该地区气象条件的变化特征及大气环境容量的时空分布特征,并对大气环境容量与气象要素之间的相关性进行了分析。结果显示:呼包鄂地区大气环境容量1月的最小、4月的最大,春季的>秋季的>夏季的>冬季的,年平均大气环境容量为62.4 t/d/km^2;月、季、年平均大气环境容量呈波动减小趋势,特别是20世纪70年代末期之后减小趋势更为显著;有明显的空间分布特征,由城区向郊区逐渐增大,城郊差异冬季最为明显,夏季差异最小;大气环境容量与降水相关性差,与风速和日最大混合层高度呈显著的正相关关系,特别是与风速相关性更显著,与小风日数呈显著的负相关关系。 相似文献
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基于TIGGE资料中欧洲中期天气预报中心、日本气象厅、美国国家环境预报中心及英国气象局1~7 d日降水量预报以及中国自动站观测资料与CMORPH降水产品融合的逐时降水量网格数据集,利用频率匹配法(Frequency-Matching Method,FMM)对中国降水预报进行客观订正。首先利用卡尔曼滤波方法对降水频率进行调整,并根据不同区域降水强度差异将全国分为7个子区域分别进行频率匹配。结果表明,FMM可以有效减小降水量预报的误差。经过频率匹配法订正后各模式降水预报的平均绝对误差(Mean Absolute Error,MAE)大幅减小,且订正后各量级降水的雨区面积更加接近实际观测值。FMM对小于5 mm和大于15 mm的降水预报技巧改进明显。此外,FMM降低了模式预报的小雨空报率和大雨漏报率,并且明显提高了晴雨预报的准确率。FMM明显消除了大范围小雨空报区域,但是对强降水预报FMM仅能调整降水量大小,强降水落区预报并不能得到明显改善。 相似文献
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