Optimization of the purification methods for recovery of recombinant growth hormone from Paralichthys olivaceus     

ZANG Xiaonan  ZHANG Xuecheng  MU Xiaosheng  LIU Bin 《中国海洋大学学报(英文版)》2013,12(1):169-174

This study aimed to optimize the purification of recombinant growth hormone from Paralichthys olivaceus. Recombinant flounder growth hormone (r-fGH) was expressed by Escherichia coli in form of inclusion body or as soluble protein under different inducing conditions. The inclusion body was renatured using two recovery methods, i.e., dilution and dialysis. Thereafter, the refolded protein was purified by Glutathione Sepharase 4B affinity chromatography and r-fGH was obtained by cleavage of thrombin. For soluble products, r-fGH was directly purified from the lysates by Glutathione Sepharase 4B affinity chromatography. ELISA-receptor assay demonstrated that despite its low receptor binding activity, the r-fGH purified from refolded inclusion body had a higher yield (2.605 mgL-1) than that from soluble protein (1.964 mgL-1). Of the tested recovery methods, addition of renaturing buffer (pH 8.5) into denatured inclusion body yielded the best recovery rate (17.9%). This work provided an optimized purification method for high recovery of r-fGH, thus contributing to the application of r-fGH to aquaculture.  相似文献   

起伏地表采集数据的三维直接叠前时间偏移方法   总被引：4，自引：1，他引：3       下载免费PDF全文

张浩  张剑锋 《地球物理学报》2012,55(4):1335-1344

提出一种可对起伏地表采集的三维地震资料直接进行偏移成像的叠前时间偏移方法和流程.它用两个等效速度描述近地表和上覆层对地震波传播的影响,可对炮、检点不在同一水平面的三维地震资料直接进行叠前时间偏移处理.该方法不对近地表地震波传播做垂直出、入射假定,因此可适应高速层出露等不存在明显低、降速带情况.描述近地表和上覆层的两个等效速度参数可依据偏移道集的同相轴是否平直来确定,避免了确定近地表速度的困难;而对已知近地表速度的情况,则可进一步修正近地表速度,获得更好的成像效果.用三维起伏地表的理论数据和中国东部某工区实际数据验证了所发展方法和处理流程的有效性和实用性.  相似文献   

海岸效应对近海地区大地电磁测深数据畸变作用研究   总被引：4，自引：3，他引：1  

张帆  魏文博  金胜  叶高峰  景建恩  张乐天  董浩  谢成良  王辉 《地球物理学报》2012,55(12):4023-4035

在近海地区采集的大地电磁测深数据通常受到海岸效应的影响,使得大地电磁测深数据发生畸变,因而很难利用大地电磁测深资料较为可靠地获得地下深部的电性结构.本文通过正演模拟方法,分析和总结海水深度变化和海底地形变化对近海地区大地电磁测深数据的畸变影响.当测区与海岸线的距离小于目标频率的大地电磁场趋肤深度时,高导海洋的存在会严重影响测区内电磁场的分布.由于海岸效应的影响,大地电磁测深视电阻率曲线和相位曲线均会发生不同程度的畸变,在低频部分,这种畸变作用尤为明显.大地电磁测深一维Occam反演方法和二维非线性共轭梯度反演方法,对近海地区浅部地层具有较好的反演效果.随着海水深度的增加和海底地形的复杂变化,两种反演方法均会出现不同程度的假异常,为地质解释工作造成了影响.近渤海地区的实测大地电磁测深数据在低频部分可能受到海岸效应的影响而导致视电阻率曲线的严重畸变.  相似文献   

Effects of nitrate on nitrite toxicity to Microcystis aeruginosa     

Chen W  Tong H  Liu H 《Marine pollution bulletin》2012,64(6):1106-1111

The aim of this study was to investigate the effect of nitrate on nitrite toxicity to Microcystis aeruginosa. Short-term uptake experiment revealed nitrate could depress the nitrite active uptake. The long-term experiment of nitrate effects on nitrite toxicity showed a negative relationship between the growth and intracellular nitrite levels of M. aeruginosa. The net nitrite uptake displayed a decline in the range of 0-150 mg NO(3)(-)-NL(-1) and an elevation at 200mg NO(3)(-)-NL(-1), whereas the activities of nitrate reductase (NR) were more sensitive to nitrate than nitrite reductase (NiR). As a result, the total intracellular nitrite levels tended to decrease up to 100 mg NO(3)(-)-NL(-1) then increased at 150-200 mg NO(3)(-)-NL(-1). These results suggested the existence of external nitrate could affect the toxicity of nitrite to alga through changing intracellular nitrite content.  相似文献   

Experimental investigation of spatially varying effect of ground motions on bridge pounding     

Bo Li  Kaiming Bi  Nawawi Chouw  John W. Butterworth  Hong Hao 《地震工程与结构动力学》2012,41(14):1959-1976

Pounding between adjacent bridge structures with insufficient separation distance has been identified as one of the primary causes of damage in many major earthquakes. It takes place because the closing relative movement is larger than the structural gap provided between the structures. This relative structural response is controlled not only by the dynamic properties of the participating structures but also by the characteristics of the ground excitations. The consequence of the spatial variation of ground motions has been studied by researchers; however, most of these studies were performed numerically. The objective of the present research is to experimentally evaluate the influence of spatial variation of ground motions on the pounding behaviour of three adjacent bridge segments. The investigation is performed using three shake tables. The input spatially varying ground excitations are simulated based on the New Zealand design spectra for soft soil, shallow soil and strong rock using an empirical coherency loss function. Results confirm that the spatially nonniform ground motions increase the relative displacement of adjacent bridge girders and pounding forces. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   

中国西部及邻区活动地块边界带现代构造应力场   总被引：8，自引：1，他引：7       下载免费PDF全文

郝平  吕晓健  田勤俭  傅征祥  陈丹 《地震学报》2012,34(4):439-450

利用哈佛全球矩心矩张量解数据和许忠淮认为1920mdash;1999年可靠的中国大陆震源机制解数据, 反演了中国西部及邻区活动地块边界带上现代构造应力场．通过对FMSI反演程序多次的输入和检验, 得到了边界带上的应力场．边界带上最大主压应力sigma;1轴绝大多数近水平. 在90deg;E以西的中国西部大陆及邻区, sigma;1轴水平方向基本上为近SN向;在青藏高原的东北部, sigma;1轴水平方向基本上为近NE向;在青藏高原的东南部, sigma;1轴水平方向绕喜马拉雅构造东端顺时针方向旋转．最小主压应力sigma;3轴倾角呈两极分布,西域地块区内活动地块边界带和青藏地块区内东北缘部分段sigma;3轴倾角较陡, 而青藏地块区内sigma;3轴倾角近水平, 所以西域地块区和青藏地块区内东北部相对于其它大部分青藏地块区, 有更多的逆冲地震．应力场在同一个边界带具有非均匀性. 北天山带、南天山带、西秦岭mdash;德令哈带、岷山mdash;龙门山带和安宁河mdash;小江带的非均匀性相对要小一些, 西昆仑带、海原mdash;祁连带、东昆仑带、玛尼mdash;玉树带、澜沧江带和滇西西边界带的非均匀性相对要大, 而喀喇昆仑mdash;嘉黎带和喜马拉雅带的非均匀性最显著．由于震源机制解数据的限制, 本文给出的是边界带上部分段的应力场．   相似文献   

安庆4.8级地震余震的波速比和波谱异常分析     

李发  戚浩  郁见芳 《山西地震》2012,(3):4-6,12

利用安徽数字地震台网安庆台记录的地震波形资料,采用单台法,计算2011年1月19日安徽安庆4.8级地震余震的波速比及利用Matlap程序计算其卓越频率,得出以下分析结果:1)波速比存在高低值异常分布,低值地震沿断裂带分布,对应地震的高频度、高强度阶段,表明该地震序列是在波速比低值异常状态下发生;处于序列后半段的余震,波速比则呈现整体高值状态,并沿区域应力场主压应力方向分布,可能是受到震源区应力调整的影响。而整个序列波速比的平均值为1.740与该区域背景值1.730基本一致,可能反映了该区域基本处于较稳定状态,发生较大地震的可能性不大。2)主震后,波速比开始上升,几次较大余震前,均呈下降的趋势,震后开始回升。3)主震后,纵横波卓越频率比迅速下降,几次较大余震前均呈现异常低值(<1.0)的状态,之后上升的过程中发生较大余震。这些结果可为较大余震的预测提供参考。  相似文献   

Bed morphology and grain size characteristics around a spur dyke     

Hao ZHANG  Hajime NAKAGAWA  Hideaki MIZUTANI 《国际泥沙研究》2012,27(2):141-155,157,156

This paper presents an experimental study on the local bed morphology and grain size characteristics around an impermeable spur dyke.A series of experiments are conducted with different types of sedime...  相似文献   

形变仪器影响观测质量因素   总被引：3，自引：2，他引：1  

卢双苓  于庆民  郝军丽  赵小贺  王帅合  毛玉华 《地震地磁观测与研究》2012,33(5):261-266

通过对泰安地震台形变仪器观测资料质量的统计分析,发现影响观测质量的主要因素包括：观测环境的改善、优化可以提高观测精度;仪器管理和数据处理水平可以影响数据精度;降雨、吸潮等可以对曲线趋势变化造成影响;环境恶化,如外界抽水等干扰,会使曲线日变固体潮失真,从而掩盖真实的地壳信息。  相似文献   

接收台阵、震源阵的传递函数与双聚束法的初步研究     

郝春月  牟磊育  郑重  张爽 《地震地磁观测与研究》2012,33(5):79-87

根据汶川地震序列,选出距离合适、波形相关性较好的事件作为震源阵,联合永久台阵(那曲、和田台阵),进行震源阵与固定台阵组合研究,计算其台阵响应.结果表明,地震序列可以形成震源阵,按照地震台阵的特殊方法进行研究,可以提高信噪比,为揭示地下精细结构提供条件.  相似文献