Reliable Procedure for Molecular Analysis of Airborne Microflora in Three Indoor Environments: An Office and Two Different Museum Contexts     

Carole Gaüzère  Marina Moletta‐Denat  Faisl Bousta  Stéphane Moularat  Geneviève Orial  Sébastien Ritoux  Jean‐Jacques Godon  Enric Robine 《洁净——土壤、空气、水》2013,41(3):226-234

Biological aerosols from air constitute a significant source of exposure to microorganisms in public places. Airborne microorganisms are involved in the development of certain respiratory symptoms, allergies, or infections among users and occupants. Various sampling instruments have commonly been used in aerobiology to collect bacteria and fungi suspended in the air. The objective of this study was to develop a reliable procedure for sampling in indoor public environments presenting different levels of occupancy, airborne bacteria and fungi to be subjected to molecular analysis (bacteria and fungi quantitative PCR, capillary electrophoresis single strand conformation polymorphism fingerprinting). Four different sampling devices were tested in situ in an office building (open‐plan type) and the sampling strategy chosen was tested in two museum contexts. In accordance with the drawbacks involved to our study (quantitative and qualitative aspects, cost, and overcrowding), cyclone device appeared to be most suitable. The results underline the effectiveness of this high‐volume aerosol sampling device for both qualitative and quantitative molecular analysis. Four in situ sampling collections were carried out in 1 day in the Louvre Museum to study quantitative and qualitative variations of airborne bacterial and fungal diversity. The quantitative results revealed a similar order of magnitude for the numbers of both bacteria and fungi. In the Louvre Museum, the samples yielded between 3.7 × 10⁴ and 4.1 × 10⁴ genome equivalent (GE) bacteria/m³ air and between 5.0 × 10⁴ and 5.9 × 10⁴ GE fungi/m³ air and in the Decorative Arts Museum between, 2.1 × 10⁴ and 2.5 × 10⁴ GE bacteria/m³ air and between 1.4 × 10⁴ and 1.7 × 10⁴ GE fungi/m³ air. The results also indicate that the dominant bacterial community displayed a stable structure over a short period of time whereas dominant eukaryotic airborne community appeared more variable.  相似文献   

It starts at home? Climate policies targeting household consumption and behavioral decisions are key to low-carbon futures     

Ghislain Dubois  Benjamin Sovacool  Carlo Aall  Maria Nilsson  Carine Barbier  Alina Herrmann  Sébastien Bruyère  Camilla Andersson  Bore Skold  Franck Nadaud  Florian Dorner  Karen Richardsen Moberg  Jean Paul Ceron  Helen Fischer  Dorothee Amelung  Marta Baltruszewicz  Jeremy Fischer  Françoise Benevise  Valérie R. Louis  Rainer Sauerborn 《Chemie der Erde / Geochemistry》2012

Through their consumption behavior, households are responsible for 72% of global greenhouse gas emissions. Thus, they are key actors in reaching the 1.5 °C goal under the Paris Agreement. However, the possible contribution and position of households in climate policies is neither well understood, nor do households receive sufficiently high priority in current climate policy strategies. This paper investigates how behavioral change can achieve a substantial reduction in greenhouse gas emissions in European high-income countries. It uses theoretical thinking and some core results from the HOPE research project, which investigated household preferences for reducing emissions in four European cities in France, Germany, Norway and Sweden. The paper makes five major points: First, car and plane mobility, meat and dairy consumption, as well as heating are the most dominant components of household footprints. Second, household living situations (demographics, size of home) greatly influence the household potential to reduce their footprint, even more than country or city location. Third, household decisions can be sequential and temporally dynamic, shifting through different phases such as childhood, adulthood, and illness. Fourth, short term voluntary efforts will not be sufficient by themselves to reach the drastic reductions needed to achieve the 1.5 °C goal; instead, households need a regulatory framework supporting their behavioral changes. Fifth, there is a mismatch between the roles and responsibilities conveyed by current climate policies and household perceptions of responsibility. We then conclude with further recommendations for research and policy.  相似文献   

The Hellenic ophiolites: eastward or westward obduction of the Maliac Ocean, a discussion   总被引：1，自引：0，他引：1  

Jacky Ferrière  Frank Chanier  Pitaksit Ditbanjong 《International Journal of Earth Sciences》2012,101(6):1559-1580

Ophiolitic bodies in the Dinaro-Hellenic mountain belt are among the most important ones in the Peri-Mediterranean Alpine chains. The characteristic feature of this ophiolitic belt is its Middle to Late Jurassic age of obduction. The ophiolitic bodies form two major belts on each side of the Pelagonian zone: an east Pelagonian belt in the Vardarian domain and a Supra-Pelagonian ophiolitic belt (SPO) to the west. The different hypotheses relative to the origin of the SPO present geodynamic evolution models accounting for most of the available data: a mid-Triassic episode of rifting; a Ladinian–Jurassic episode of sea-floor spreading forming notably the Maliac Ocean; a Middle to Late Jurassic convergent period with subduction and obduction episodes, and finally, a late episode of Tertiary compressional deformation responsible for the westward thrusting of the Jurassic ophiolitic nappes over the external zones. Despite many studies dating from the early 1970s, the eastern or western Pelagonian origin of these ophiolites, especially the SPO, is still under dispute. Whatever the adopted hypothesis, we consider that the main SPO bodies (N-Pindos, Vourinos, Othris, Evia, Argolis) have the same origin because of their geographic continuity and of the similarities in their geological characteristics. We propose that this ocean corresponds everywhere to the Maliac Ocean, defined in Othris from the well-preserved sedimentary (oceanic margin) and ophiolitic nappes thrust during the Late Jurassic obduction onto the Pelagonian platform. There is strong evidence for the existence of two deep basins on both sides of the Pelagonian continental ridge during Triassic–Jurassic times. They correspond, respectively, to the Vardar area to the east and the Pindos domain to the west, one of these domains being at the origin of the SPO. The hypothesis of an eastward emplacement of the SPO from the Pindos domain is based mainly on sedimentological data from the margin series and on structural analyses of ophiolitic bodies. However, we conclude the westward obduction of the Maliac Ocean, originating from the Vardar area, to be the best fitting model. This westward model is supported by paleogeographic and structural constraints on regional scale. Notably, the absence of obducted ophiolites in the Jurassic series of the Koziakas units (units attributed to the western Pelagonian margin) and of the Parnassus domain (on the eastern side of the Pindos basin) is difficult to reconcile with an eastward obduction from the Pindos domain. Other observations, such as the distribution of ophiolitic detritus in the internal and external zones, also promote the westward Late Jurassic obduction of the Maliac Ocean. Our preferred model offers a consistent explanation for the mechanism and timing of the emplacement of the SPO, as well as providing insight on the origin and emplacement of the Vardarian ophiolites. Following this hypothesis, there is no need for a clear boundary between the SPO and the west Vardarian ophiolitic bodies as they were obducted from the same oceanic basin and during the same Jurassic tectonic event. In this paper, we develop evidence in favor of the eastern Pelagonian origin for the SPO (our adopted model) and provide discussion on the data supporting the main alternative hypothesis (western origin for the SPO).  相似文献   

Dissolution of calcitic marble and dolomitic rock in high iron concentrated acid mine drainage: application to anoxic limestone drains     

Thomas Genty  Bruno Bussière  Robin Potvin  Mostafa Benzaazoua  Gérald J. Zagury 《Environmental Earth Sciences》2012,66(8):2387-2401

Oxidation of sulphide mining waste can generate acid mine drainage (AMD) that has the potential to seriously affect the ecosystems. Acid mine drainage is characterised by a high acidity, high concentrations of sulphates and metals. To reduce the environmental impacts due to AMD, neutralisation using limestone drains is an option proposed in the literature and used around the world. The present study focuses on the influence of the carbonate rock mineralogy and their particle size on the neutralising capacity. The tests were performed in two different anoxic conditions: in batch reactors, and in columns having a hydraulic retention time of 15?h. The results showed that the neutralisation capacity of calcite was more important than for dolomitic rock, and smaller particle size gave higher alkalinity production (fine calcite dissolved faster in contact with AMD). A characterization of metal precipitate in sludge and in limestone coating was performed and demonstrated that gypsum, lepidocrocite and goethite were the predominant secondary minerals to be formed. Finally, this study underlines that anoxic limestone drain cannot be used alone to treat high iron concentrated AMD.  相似文献   

Earth-Affecting Solar Causes Observatory (EASCO): A potential International Living with a Star Mission from Sun–Earth L5     

N. Gopalswamy  J.M. Davila  O.C. St. Cyr  E.C. Sittler  F. Auchère  T.L. Duvall  J.T. Hoeksema  M. Maksimovic  R.J. MacDowall  A. Szabo  M.R. Collier 《Journal of Atmospheric and Solar》2011,73(5-6):658-663

This paper describes the scientific rationale for an L5 mission and a partial list of key scientific instruments the mission should carry. The L5 vantage point provides an unprecedented view of the solar disturbances and their solar sources that can greatly advance the science behind space weather. A coronagraph and a heliospheric imager at L5 will be able to view CMEs broadsided, so space speed of the Earth-directed CMEs can be measured accurately and their radial structure discerned. In addition, an inner coronal imager and a magnetograph from L5 can give advance information on active regions and coronal holes that will soon rotate on to the solar disk. Radio remote sensing at low frequencies can provide information on shock-driving CMEs, the most dangerous of all CMEs. Coordinated helioseismic measurements from the Sun–Earth line and L5 provide information on the physical conditions at the base of the convection zone, where solar magnetism originates. Finally, in situ measurements at L5 can provide information on the large-scale solar wind structures (corotating interaction regions (CIRs)) heading towards Earth that potentially result in adverse space weather.  相似文献   

Evidence for the enhanced lability of dissolved organic matter following permafrost slope disturbance in the Canadian High Arctic     

Gwen C. Woods  Brent G. Pautler  Melissa J. Lafrenière 《Geochimica et cosmochimica acta》2011,75(22):7226-7241

Arctic landscapes are believed to be highly sensitive to climate change and accelerated disturbance of permafrost is expected to significantly impact the rate of carbon cycling. While half the global soil organic matter (SOM) is estimated to reside in Arctic soils, projected warmer temperatures and permafrost disturbance will release much of this SOM into waterways in the form of dissolved organic matter (DOM). The spring thaw and subsequent flushing of soils releases the highest contributions of DOM annually but has historically been undersampled due to the difficulties of sampling during this period. In this study, passive samplers were placed throughout paired High Arctic watersheds during the duration of the 2008 spring flush in Nunavut, Canada. The watersheds are very similar with the exception of widespread active layer detachments (ALDs) that occurred within one of the catchments during a period of elevated temperatures in the summer of 2007. DOM samples were analyzed for structural and spectral characteristics via nuclear magnetic resonance (NMR) and fluorescence spectroscopy as well as vulnerability to degradation with simulated solar exposure. Lignin-derived phenols were further assessed utilizing copper(II) oxide (CuO) oxidation and gas chromatography/mass spectrometry (GC/MS). The samples were found to have very low dissolved lignin phenol content (∼0.07% of DOC) and appear to originate from primarily non-woody angiosperm vegetation. The acid/aldehyde ratios for dissolved vanillyl phenols were found to be high (up to 3.6), indicating the presence of highly oxidized lignin. Differences between DOM released from the ALD vs. the undisturbed watershed suggest that these shallow detachment slides have significantly impacted the quality of Arctic DOM. Although material released from the disturbed catchment was found to be highly oxidized, DOM in the lake into which this catchment drained had chemical characteristics indicating high contributions from microbial and/or primary productivity. The resulting pool of dissolved carbon within the lake appears to be more biologically- and photochemically-labile than material from the undisturbed system. These disturbances may have implications for projected climate warming; sustained elevated temperatures would likely perpetuate widespread ALDs and further affect carbon cycling in this environment.  相似文献   

TomograPy: A Fast, Instrument-Independent, Solar Tomography Software     

N. Barbey  C. Guennou  F. Auchère 《Solar physics》2013,283(1):227-245

Solar tomography has progressed rapidly in recent years thanks to the development of robust algorithms and the availability of more powerful computers. It can today provide crucial insights in solving issues related to the line-of-sight integration present in the data of solar imagers and coronagraphs. However, there remain challenges such as the increase of the available volume of data, the handling of the temporal evolution of the observed structures, and the heterogeneity of the data in multi-spacecraft studies. We present a generic software package that can perform fast tomographic inversions that scales linearly with the number of measurements, linearly with the length of the reconstruction cube (and not the number of voxels), and linearly with the number of cores and can use data from different sources and with a variety of physical models: TomograPy (ref="http://nbarbey.github.com/TomograPy/"> http://nbarbey.github.com/TomograPy/ ), an open-source software freely available on the Python Package Index. For performance, TomograPy uses a parallelized-projection algorithm. It relies on the World Coordinate System standard to manage various data sources. A variety of inversion algorithms are provided to perform the tomographic-map estimation. A test suite is provided along with the code to ensure software quality. Since it makes use of the Siddon algorithm it is restricted to rectangular parallelepiped voxels but the spherical geometry of the corona can be handled through proper use of priors. We describe the main features of the code and show three practical examples of multi-spacecraft tomographic inversions using STEREO/EUVI and STEREO/COR1 data. Static and smoothly varying temporal evolution models are presented.  相似文献   

Coronal Temperature Maps from Solar EUV Images: A Blind Source Separation Approach     

T. Dudok de Wit  S. Moussaoui  C. Guennou  F. Auchère  G. Cessateur  M. Kretzschmar  L. A. Vieira  F. F. Goryaev 《Solar physics》2013,283(1):31-47

Multi-wavelength solar images in the extreme ultraviolet (EUV) are routinely used for analysing solar features such as coronal holes, filaments, and flares. However, images taken in different bands often look remarkably similar, as each band receives contributions coming from regions with a range of different temperatures. This has motivated the search for empirical techniques that may unmix these contributions and concentrate salient morphological features of the corona in a smaller set of less redundant source images. Blind Source Separation (BSS) does precisely this. Here we show how this novel concept also provides new insight into the physics of the solar corona, using observations made by SDO/AIA. The source images are extracted using a Bayesian positive source-separation technique. We show how observations made in six spectral bands, corresponding to optically thin emissions, can be reconstructed by a linear combination of three sources. These sources have a narrower temperature response and allow for considerable data reduction, since the pertinent information from all six bands can be condensed into a single composite picture. In addition, they give access to empirical temperature maps of the corona. The limitations of the BSS technique and some applications are briefly discussed.  相似文献   

It starts at home? Climate policies targeting household consumption and behavioral decisions are key to low-carbon futures     

Ghislain Dubois  Benjamin Sovacool  Carlo Aall  Maria Nilsson  Carine Barbier  Alina Herrmann  Sébastien Bruyère  Camilla Andersson  Bore Skold  Franck Nadaud  Florian Dorner  Karen Richardsen Moberg  Jean Paul Ceron  Helen Fischer  Dorothee Amelung  Marta Baltruszewicz  Jeremy Fischer  Françoise Benevise  Rainer Sauerborn 《Chemie der Erde / Geochemistry》2013

Through their consumption behavior, households are responsible for 72% of global greenhouse gas emissions. Thus, they are key actors in reaching the 1.5 °C goal under the Paris Agreement. However, the possible contribution and position of households in climate policies is neither well understood, nor do households receive sufficiently high priority in current climate policy strategies. This paper investigates how behavioral change can achieve a substantial reduction in greenhouse gas emissions in European high-income countries. It uses theoretical thinking and some core results from the HOPE research project, which investigated household preferences for reducing emissions in four European cities in France, Germany, Norway and Sweden. The paper makes five major points: First, car and plane mobility, meat and dairy consumption, as well as heating are the most dominant components of household footprints. Second, household living situations (demographics, size of home) greatly influence the household potential to reduce their footprint, even more than country or city location. Third, household decisions can be sequential and temporally dynamic, shifting through different phases such as childhood, adulthood, and illness. Fourth, short term voluntary efforts will not be sufficient by themselves to reach the drastic reductions needed to achieve the 1.5 °C goal; instead, households need a regulatory framework supporting their behavioral changes. Fifth, there is a mismatch between the roles and responsibilities conveyed by current climate policies and household perceptions of responsibility. We then conclude with further recommendations for research and policy.  相似文献   

The SWAP EUV Imaging Telescope Part I: Instrument Overview and Pre-Flight Testing     

D. B. Seaton  D. Berghmans  B. Nicula  J.-P. Halain  A. De Groof  T. Thibert  D. S. Bloomfield  C. L. Raftery  P. T. Gallagher  F. Auchère  J.-M. Defise  E. D’Huys  J.-H. Lecat  E. Mazy  P. Rochus  L. Rossi  U. Schühle  V. Slemzin  M. S. Yalim  J. Zender 《Solar physics》2013,286(1):43-65

The Sun Watcher with Active Pixels and Image Processing (SWAP) is an EUV solar telescope onboard ESA’s Project for Onboard Autonomy 2 (PROBA2) mission launched on 2 November 2009. SWAP has a spectral bandpass centered on 17.4 nm and provides images of the low solar corona over a 54×54 arcmin field-of-view with 3.2 arcsec pixels and an imaging cadence of about two minutes. SWAP is designed to monitor all space-weather-relevant events and features in the low solar corona. Given the limited resources of the PROBA2 microsatellite, the SWAP telescope is designed with various innovative technologies, including an off-axis optical design and a CMOS–APS detector. This article provides reference documentation for users of the SWAP image data.  相似文献