Lysosomal injury and MFO activity in the liver of flounder (Platichthys flesus L.) in relation to histopathology of hepatic degeneration and carcinogenesis     

Angela Khler  Hans Jürgen Pluta 《Marine environmental research》1995,39(1-4)

Flounder (Platichthys flesus L.), 18–48 cm total length were sampled in the mouth of the Elbe River during a 3-year interdisciplinary project ‘Fish Diseases in the Wadden Sea’. Lysosomal changes (lysosomal membrane stability) and the activity of biotransforming enzymes (MFOs/EROD) were measured parallel to investigations of ultrastructural changes in liver cells, tissue pathologies and macroscopically visible changes. The aim of the study was to investigate if these cyto- and biochemical parameters were able to reflect contaminant induced biological effects. Interlinking of the results of the MFO activity to the pathological alterations observed at the electron- and light-microscopic levels as well as during macroscopic inspection of identical individuals evidenced that the activity of the biotransformation enzymes (EROD) was not induced in healthy livers, increased considerably with the onset of liver changes and dropped again in those livers with degenerative, preneoplastic and neoplastic lesions. In contrast, concurrent studies of lysosomal membrane stability measured in parallel showed a decrease with the onset and progression of liver lesions from reversible to irreversible, neoplastic change.  相似文献   

Optimisation of a catalytic procedure for the determination of total iodine in seawater     

Victor W. Truesdale  Piers Chapman 《Marine Chemistry》1976,4(1):29-42

A precise method for the determination of total iodine in seawater, which uses a Technicon Auto-Analyser II system and is based on Truesdale and Spencer's (1974) and Truesdale and Smith's (1975) earlier works with a catalytic procedure, is described. The procedure operates over a range of 30–80 μg1^?1 of iodine. As requested by Mark (1973), the way in which the procedure can be varied to suit other applications, and therefore ranges, is explained in detail. The standard deviation at the 50 μg1^?1 level was found to be 0.12 μ1^?1 showing that the method's precision exceeds most, if not all, previously published procedures for this variable. This high degree of resolution makes the procedure suitable for oceanic investigations. The effects of varying the reaction time, the temperature at which the reaction proceeds and the salinity of samples and standards are described. The advantages of using a Technicon Auto-Analyser II system rather than an Auto-Analyser I system are discussed. With slight modification the procedure can also be used in the measurement of river flow where sodium iodide is used as tracer in the dilution method.  相似文献   

Effects of crustacean traps on benthic fauna     

Eno  N. Clare; MacDonald  David S.; Kinnear  Jim A. M.; Amos  S. Chris; Chapman  Colin J.; Clark  Robin A.; Bunker  Francis St P.D.; Munro  Colin 《ICES Journal of Marine Science》2001,58(1):11-20

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Variability of organic δC and C/N in the Mersey Estuary, U.K. and its implications for sea-level reconstruction studies     

Graham P. Wilson  Angela L. Lamb  Melanie J. Leng  Silvia Gonzalez  David Huddart 《Estuarine, Coastal and Shelf Science》2005,64(4):685-698

Microfossil analysis (e.g. diatoms, foraminifera and pollen) represents the cornerstone of Holocene relative sea-level (RSL) reconstruction because their distribution in the contemporary inter-tidal zone is principally controlled by ground elevation within the tidal frame. A combination of poor microfossil preservation and a limited range in the sediment record may severely restrict the accuracy of resulting RSL reconstructions. Organic δ¹³C and C/N analysis of inter-tidal sediments have shown some potential as coastal palaeoenvironmental proxies. Here we assess their viability for reconstructing RSL change by examining patterns of organic δ¹³C and C/N values in a modern estuarine environment. δ¹³C and C/N analysis of bulk organic inter-tidal sediments and vegetation, as well as suspended and bedload organic sediments of the Mersey Estuary, U.K., demonstrate that the two main sources of organic carbon to surface saltmarsh sediments (terrestrial vegetation and tidal-derived particulate organic matter) have distinctive δ¹³C and C/N signatures. The resulting relationship between ground elevation within the tidal frame and surface sediment δ¹³C and C/N is unaffected by decompositional changes. The potential of this technique for RSL reconstruction is demonstrated by the analysis of part of an early Holocene sediment core from the Mersey Estuary. Organic δ¹³C and C/N analysis is less time consuming than microfossil analysis and is likely to provide continuous records of RSL change.  相似文献   

Effects of ship motion on a neutrally-stable towed fish     

D.A. Chapman 《Ocean Engineering》1982,9(3):189-220

Digital computer simulation in two dimensions of a neutrally-stable fish towed on a faired cable has been used to investigate the response of the fish to disturbances in the ship motion. The numerical methods used are described broadly, excluding mathematical detail, and selected results are presented which indicate typical behaviour of the system.The free oscillations of the fish-cable system and its response to deterministic and random ship disturbances are investigated. This reveals the dynamic principles governing the motion and allows the formulation of simple rules to give a first approximation to the magnitude of the fish response. It is shown that, provided resonances of the ship and fish-cable system do not coincide, the overall amplitude of motion of the fish is proportional to the sine of the angle made by the top of the cable with the horizontal. Formulae are given for calculating approximately the resonant frequencies of the towed system.  相似文献   

Diel vertical behaviour, predator-prey relationships, and occupation of space by jack mackerel (Trachurus murphyi) off Chile   总被引：3，自引：0，他引：3  

Bertrand  Arnaud; Barbieri  Maria Angela; Cordova  Jose; Hernandez  Carola; Gomez  Fabian; Leiva  Francisco 《ICES Journal of Marine Science》2004,61(7):1105-1112

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A seasonal survey of the sterol composition of the cockle cerastoderma edule: A comparison of polluted and non-polluted environments     

James A Ballantine  Angela Lavis  Robert J Morris 《Marine environmental research》1983,9(2):111-122

The sterol composition of a cockle population from an unpolluted environment in South Wales was monitored by monthly sampling over a 12-month period. In spite of the likely variation in dietary sterols during this period, the component sterols of the cockles showed no significant seasonal variation, indicating that the cockle was able to exhibit a high degree of control over the composition of its functional sterols.Dramatic differences were observed in the sterol compositions of a nearby cockle population from a highly polluted environment, near a sewage outfall; only three out of the ten sterols present were common to the unpolluted cockles. Forty-five per cent of the sterols from the ‘polluted’ samples contained fully reduced systems and included three new alkylated C-29, C-30 and C-31 stanols. It is considered possible that these structural changes may affect the stereochemistry of the functional sterols and hence alter the permeability of the animal's membranes. This may reflect a biochemical response to the polluted environment.  相似文献   

Towed cable behaviour during ship turning manoeuvers     

D.A. Chapman 《Ocean Engineering》1984,11(4):327-361

The results of dynamic and steady-state cable simulations are used to show that a towed system can behave in either of two different ways on entering a turn. In a large radius turn the system maintains its straight-tow equilibrium configuration but in a slightly perturbed form. In a turn of small radius the system effectively collapses resulting in a large increase of fish depth and cable tension. A formula is included by which the approximate minimum radius of turn that does not precipitate collapse can be quickly calculated.Non-dimensional tables are presented giving details of the equilibrium configuration adopted by the cable when the ship maintains a circular course.Graphs are presented from which the time constants for the decay of lateral and longitudinal disturbances of 2-D cable profiles can be easily calculated. These can be used to estimate the time taken for a towed system to return to equilibrium following a manoeuvre.The derivation of the equations for the steady-state configurations and the time constants are included in appendices.  相似文献   

Comment on “Spherical harmonic analysis of Earth’s conductive heat flow” by V. M. Hamza, R. R. Cardoso and C. F. Ponte Neto     

Henry N. Pollack  David S. Chapman 《International Journal of Earth Sciences》2008,97(2):227-231

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An analysis of 1983 observations of facular contrast with an extreme limb photometer     

G. A. Chapman  B. Ziegler 《Solar physics》1996,168(2):259-272

Analysis of facular contrast I/I _qs from Extreme Limb Photometer (ELP) data of the summer of 1983 yield a mean contrast of 0.91 ± 0.19% and 1.57 ± 0.16% for apertures 1 and 2 located at = 0.198 and at = 0.111, respectively. The ratio of the mean contrast in the outer aperture (closer to the limb) to that of the inner one is 1.71 ± 0.40, indicating an increase in the mean facular contrast toward the limb. This result is in agreement with observations made in 1975, 1979, and 1982. The errors are dominated by the random presence of solar active regions. The combined results from all seasons follow an approximately ^–1 curve. Facular excess solar oblateness signals for 1983 are 33.8 ± 6.6 arc ms and 16.5 ± 2.1 arc ms for ELP apertures 1 and 2, respectively, in reasonable agreement with the 1983 excess solar oblateness results of Dicke, Kuhn, and Libbrecht (1985).  相似文献