Use of molecular approaches in hydrogeological studies: the case of carbonate aquifers in southern Italy     

Antonio Bucci  Emma Petrella  Fulvio Celico  Gino Naclerio 《Hydrogeology Journal》2017,25(4):1017-1031

Waterborne pathogens represent a significant health risk in both developed and developing countries with sensitive sub-populations including children, the elderly, neonates, and immune-compromised people, who are particularly susceptible to enteric infections. Annually, approximately 1.8 billion people utilize a faecally contaminated water source, and waterborne diseases are resulting in up to 2.1 million human mortalities globally. Although groundwater has traditionally been considered less susceptible to contamination by enteric pathogens than surface water due to natural attenuation by overlying strata, the degree of microbial removal attributable to soils and aquifers can vary significantly depending on several factors. Thus, accurate assessment of the variable presence and concentration of microbial contaminants, and the relative importance of potentially causative factors affecting contaminant ingress, is critical in order to develop effective source (well) and resource (aquifer) protection strategies. “Traditional” and molecular microbiological study designs, when coupled with hydrogeological, hydrochemical, isotopic, and geophysical methods, have proven useful for analysis of numerous aspects of subsurface microbial dynamics. Accordingly, this overview paper presents the principal microbial techniques currently being employed (1) to predict and identify sources of faecal contamination in groundwater, (2) to elucidate the dynamics of contaminant migration, and (3) to refine knowledge about the hydrogeological characteristics and behaviours of aquifer systems affected by microbial contamination with an emphasis on carbonate aquifers, which represent an important global water supply. Previous investigations carried out in carbonate aquifers in southern Italy are discussed.  相似文献   

The roles of flowering,overwinter survival and sea surface temperature in the long-term population dynamics of Zostera marina around the Isles of Scilly,UK     

Maria Potouroglou  Emma J. Kenyon  Angie Gall  Kevan J. Cook  James C. Bull 《Marine pollution bulletin》2014

Interaction between biotic and abiotic drivers of dynamics is an important topic in ecology. Despite numerous short-term studies, there is a paucity of evidence about how environmental structure modifies dynamics in marine systems. We quantified Zostera marina flowering and non-flowering shoot density annually from 1996 to 2012 around the Isles of Scilly, UK, parameterizing a population dynamic model. Flowering is structured in time and space, with temperature and flowering positively associated at some locations only. We found no evidence that flower production contributes to seagrass density but ‘patchiness’ was positively associated with flowering in the previous year. With evidence of substantial overwinter survival, findings support the hypothesis that local populations are maintained largely through vegetative reproduction but sexual reproduction may contribute to colonisation of vacant habitat. This long-term study (1) tests validity of shorter-term investigations, (2) quantifies interaction between biotic and abiotic factors and (3) promotes seagrass as a model ecosystem.  相似文献   

Bacterial communities are sensitive indicators of contaminant stress     

Sun MY  Dafforn KA  Brown MV  Johnston EL 《Marine pollution bulletin》2012,64(5):1029-1038

With many environments worldwide experiencing at least some degree of anthropogenic modification, there is great urgency to identify sensitive indicators of ecosystem stress. Estuarine organisms are particularly vulnerable to anthropogenic contaminants. This study presents bacterial communities as sensitive indicators of contaminant stress. Sediments were collected from multiple sites within inner and outer zones of three heavily modified and three relatively unmodified estuaries. Bacterial communities were censused using Automated Ribosomal Intergenic Spacer Analysis and analysed for a suite of metal and PAH contaminants. Shifts in both bacterial community composition and diversity showed strong associations with sediment contaminant concentrations, particularly with metals. Importantly, these changes are discernable from environmental variation inherent to highly complex estuarine environments. Moreover, variation in bacterial communities within sites was limited. This allowed for differences between sites, zones and estuaries to be explained by variables of interest such as contaminants that vary between, but not within individual sites.  相似文献   

Improving marine environmental health through marine citizenship: A call for debate     

Emma McKinley  Stephen Fletcher 《Marine Policy》2012,36(3):839-843

This paper presents an evaluation of the role of marine citizenship in improving marine environmental health and marine governance. Marine citizenship describes the rights and responsibilities of an individual towards the marine environment, which support the achievement of marine policy objectives at the national level. It is argued that marine citizenship requires an enhanced awareness of marine environmental issues, an understanding of the role of personal behaviour in creating and resolving marine environmental issues, and a shift in values to promote marine pro-environmental behavioural choices. It is concluded that the value shift is likely to be produced by the development of an altered relationship between the state and the individual, in which the expectations placed on citizens by the state are extended to include marine pro-environmental behaviour. The paper is intended to stimulate debate and the authors invite and encourage replies to the ideas contained within the paper.  相似文献   

High‐frequency water quality monitoring in an urban catchment: hydrochemical dynamics,primary production and implications for the Water Framework Directive          下载免费PDF全文

Sarah J. Halliday  Richard A. Skeffington  Andrew J. Wade  Michael J. Bowes  Emma Gozzard  Jonathan R. Newman  Matthew Loewenthal  Elizabeth J. Palmer‐Felgate  Helen P. Jarvie 《水文研究》2015,29(15):3388-3407

This paper describes the hydrochemistry of a lowland, urbanised river‐system, The Cut in England, using in situ sub‐daily sampling. The Cut receives effluent discharges from four major sewage treatment works serving around 190 000 people. These discharges consist largely of treated water, originally abstracted from the River Thames and returned via the water supply network, substantially increasing the natural flow. The hourly water quality data were supplemented by weekly manual sampling with laboratory analysis to check the hourly data and measure further determinands. Mean phosphorus and nitrate concentrations were very high, breaching standards set by EU legislation. Although 56% of the catchment area is agricultural, the hydrochemical dynamics were significantly impacted by effluent discharges which accounted for approximately 50% of the annual P catchment input loads and, on average, 59% of river flow at the monitoring point. Diurnal dissolved oxygen data demonstrated high in‐stream productivity. From a comparison of high frequency and conventional monitoring data, it is inferred that much of the primary production was dominated by benthic algae, largely diatoms. Despite the high productivity and nutrient concentrations, the river water did not become anoxic, and major phytoplankton blooms were not observed. The strong diurnal and annual variation observed showed that assessments of water quality made under the Water Framework Directive (WFD) are sensitive to the time and season of sampling. It is recommended that specific sampling time windows be specified for each determinand, and that WFD targets should be applied in combination to help identify periods of greatest ecological risk. © 2015 The Authors. Hydrological Processes published by John Wiley & Sons Ltd.  相似文献   

Rethinking communication in risk interpretation and action     

Shabana Khan  Jyoti L. Mishra  Kuna-hui Elaine Lin  Emma E. H. Doyle 《Natural Hazards》2017,85(3):1709-1722

We have studied the attenuation characteristics of eastern Himalaya and southern Tibet by using local earthquake data set that consists of 123 well-located events, recorded by the Himalayan Nepal Tibet Seismic Experiment operated during 2001–2003. We have used single backscattering model to calculate frequency-dependent values of coda Q (\(Q_\mathrm{c}\)). The estimation of \(Q_\mathrm{c}\) is made at central frequencies 2, 4, 8 and 12 Hz through five lapse time windows from 10 to 50 s starting at double the travel time of the S-wave. The observed \(Q_\mathrm{c}\) is found to be strongly frequency-dependent and follows a similar trend as observed in other tectonically active parts of the Himalaya. The trend of variation of \(Q_\mathrm{c}\) with lapse time and the corresponding apparent depths is also studied. Increase in \(Q_\mathrm{c}\) values with the lapse time suggests that the deeper part of the study region is less heterogeneous than the shallower part. The observed values of \(Q_0\) (\(Q_\mathrm{c}\) at 1 Hz) and frequency parameter n indicate that the medium beneath the study area is highly heterogeneous and tectonically very active. A regionalization of the estimated \(Q_0\) is carried out, and a contour map is prepared for the whole region. Some segments of Lesser Himalaya and Sub-Himalaya exhibit very low \(Q_0\) , while the whole Tethyan Himalaya and some parts of Greater Himalaya are characterized by low \(Q_0\) values. Our results are comparable with those obtained from tectonically active regions in the world.  相似文献   

Governing resilience building in Thailand's tourism-dependent coastal communities: Conceptualising stakeholder agency in social-ecological systems     

Rasmus Klocker Larsen  Emma CalgaroFrank Thomalla 《Global Environmental Change》2011,21(2):481-491

In current scientific efforts to harness complementarity between resilience and vulnerability theory, one response is an ‘epistemological shift’ towards an evolutionary, learning based conception of the ‘systems-actor’ relation in social-ecological systems. In this paper, we contribute to this movement regarding the conception of stakeholder agency within social-ecological systems. We examine primary evidence from the governance of post-disaster recovery and disaster risk reduction efforts in Thailand's coastal tourism-dependent communities following the 2004 Indian Ocean Tsunami. Through an emerging storyline from stakeholders, we construct a new framework for conceptualising stakeholder agency in social-ecological systems, which positions the notion of resilience within a conception of governance as a negotiated normative process. We conclude that if resilience theory is proposed as the preferred approach by which disaster risk reduction is framed and implemented, it needs to acknowledge much more explicitly the role of stakeholder agency and the processes through which legitimate visions of resilience are generated.  相似文献   

Ostreopsis cf. ovata bloom in the northern Adriatic Sea during summer 2009: ecology, molecular characterization and toxin profile     

Accoroni S  Romagnoli T  Colombo F  Pennesi C  Di Camillo CG  Marini M  Battocchi C  Ciminiello P  Dell'Aversano C  Dello Iacovo E  Fattorusso E  Tartaglione L  Penna A  Totti C 《Marine pollution bulletin》2011,62(11):2512-2519

Intense blooms of the benthic dinoflagellate Ostreopsis cf. ovata have occurred in the northern Adriatic Sea since 2006. These blooms are associated with noxious effects on human health and with the mortality of benthic organisms because of the production of palytoxin-like compounds. The O. cf. ovata bloom and its relationships with nutrient concentrations at two stations on the Conero Riviera (northern Adriatic Sea) were investigated in the summer of 2009. O. cf. ovata developed from August to November, with the highest abundances in September (1.3 × 10⁶ cells g⁻¹ fw corresponding to 63.8 × 10³ cells cm⁻²). The presence of the single O. cf. ovata genotype was confirmed by a PCR assay. Bloom developed when the seawater temperature was decreasing. Nutrient concentrations did not seem to affect bloom dynamics. Toxin analysis performed by high resolution liquid chromatography-mass spectrometry revealed a high total toxin content (up to 75 pg cell⁻¹), including putative palytoxin and all the ovatoxins known so far.  相似文献   

Potential risk of harmful algae transport by ballast waters: the case of Bilbao Harbour   总被引：1，自引：0，他引：1  

Butrón A  Orive E  Madariaga I 《Marine pollution bulletin》2011,62(4):747-757

Ballast water exchange was measured for the first time in Bilbao Harbour, one of the most active of northern Spain. Between 1997 and 2006, 41,900,980.34 ballast water tn were loaded and 13,272,709 tn were discharged. Bilbao Harbour appears to be mainly a source of ballast water, 90% of which would be discharged in European harbours. We estimated that vessels carrying liquid and solid bulk have higher probabilities of exporting ballast water, whereas those with liquid bulk and containers are more likely to introduce it. From 30 potentially harmful phytoplankton species identified to date near harbour facilities, there would be a high risk of exporting at least Alexandrium minutum, Dinophysis sp., Heterosigma akashiwo, Karlodinium sp., Ostreopsis cf. siamensis, Pfiesteria-like and Prorocentrum minimum. Invasion risk by ballast water was tested by analyzing the response of six strains of H. akashiwo from different geographic areas to varying salinity. Results show that successful growth of foreign strains would be possible.  相似文献   

A lacustrine GDGT-temperature calibration from the Scandinavian Arctic to Antarctic: Renewed potential for the application of GDGT-paleothermometry in lakes   总被引：2，自引：0，他引：2  

Emma J. Pearson  Steve Juggins  Jan Weckström  David B. Ryves  Roland Schmidt 《Geochimica et cosmochimica acta》2011,75(20):6225-6238

Quantitative climate reconstructions are fundamental to understand long-term trends in natural climate variability and to test climate models used to predict future climate change. Recent advances in molecular geochemistry have led to calibrations using glycerol dialkyl glycerol tetraethers (GDGTs), a group of temperature-sensitive membrane lipids found in Archaea and bacteria. GDGTs have been used to construct temperature indices for oceans (TEX₈₆ index) and soils (MBT/CBT index). The aim of this study is to examine GDGT-temperature relationships and assess the potential of constructing a GDGT-based palaeo-thermometer for lakes. We examine GDGT-temperature relationships using core top sediments from 90 lakes across a north-south transect from the Scandinavian Arctic to Antarctica including sites from Finland, Sweden, Siberia, the UK, Austria, Turkey, Ethiopia, Uganda, Chile, South Georgia and the Antarctic Peninsula. We examine a suite of 15 GDGTs, including compounds used in the TEX₈₆ and MBT/CBT indices and reflecting the broad range of GDGT inputs to small lake systems.GDGTs are present in varying proportions in all lakes examined. The TEX₈₆ index is not applicable to our sites because of the large relative proportions of soil derived and methanogenic components. Similarly, the MBT/CBT index is also not applicable and predicts temperatures considerably lower than those measured. We examine relationships between individual GDGT compounds and temperature, pH, conductivity and water depth. Temperature accounts for a large and statistically independent fraction of variation in branched GDGT composition. We propose a GDGT-temperature regression model with high accuracy and precision (R² = 0.88; RMSE = 2.0 °C; RMSEP = 2.1 °C) for use in lakes based on a subset of branched GDGT compounds and highlight the potential of this new method for reconstructing past temperatures using lake sediments.  相似文献