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Seventeen lactic acid bacterial strains (LAB) were isolated using MRS agar medium from Jeotgal, a Korean fermented food, purchased
at the Jukdo market of Pohang. To identify the strains isolated, they were tested by examining their cell morphologies, gram-staining,
catalase activity, arginine hydrolase activity, D-L lactate form and carbohydrate fermentation. According to the phenotypic
characteristics, three strains were tent atively identified asLactobacillus spp., ten wereEnterococcus spp. (orStreptococcus spp., orPediococcus spp.) and the rest were Leuconostoc spp. (orWeissella spp.). Five strains among 17 were chosen by preliminary bacteriocin activity test. Four bacterial strains which inhibited
both indicator microorganisms were identified by 16S rRNA sequencing. The results are as follows; Leuconostoc mesenteroides
(HK 4),Leuconostoc mesenteroides (HK 5),Leuconostoc mesenteroides(HK 11),Streptococcus salivarius(HK 8). In order to check LAB which are showing a high survival rate in gut, we investigated three strains inhibiting both
indicator microorganisms in artificial gastric acid and bile juice -all except HK8. The three strains mentioned above grew
in extreme low acid conditions. 相似文献
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A GPS-slaved time synchronization system for hybrid navigation 总被引：4，自引：1，他引：4
For multisensor integration, a primary prerequisite is time synchronization. Methods that have been used in many operational systems are either too expensive, or have inconvenient features, that make them inappropriate for research purposes. In this paper, the system design of a cost effective synchronization system (CESS) is described. Tests show that a synchronization accuracy of 0.4 ms can be achieved using this system. A land trial of GPS/INS integration verified that the CESS is accurate enough for most low-cost sensor integration applications. However, a more accurate synchronization can be achieved by minor modifications to the CESS. This system provides an ideal platform for researchers to build their own integrated multisensor systems. 相似文献
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Post‐repair effect of column jackets on aftershock fragilities of damaged RC bridges subjected to successive earthquakes 
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下载免费PDF全文 In light of recent earthquakes, structures damaged during an initial seismic event (mainshock) may be more vulnerable to severe damage and collapse during a subsequent event (aftershock). In this paper, a framework for the development of aftershock fragilities is presented; these aftershock fragilities define the likelihood that a bridge damaged during an initial event will exhibit a given damage state following one or more subsequent events. The framework is capable of (i) quantifying the cumulative damage of unrepaired bridges subjected to mainshock–aftershock sequences (effect of multiple earthquakes) and (ii) evaluating the effectiveness of column repair schemes such as steel and fiber‐reinforced‐polymer jackets (post‐repair effect of jackets). To achieve this aim, the numerical model of repaired columns is validated using existing experimental results. A non‐seismically designed bridge is chosen as a case study and is modeled for three numerical bridge models: a damaged (but unrepaired) bridge model, and two bridge models with columns repaired with steel and fiber‐reinforced polymer jackets. A series of back‐to‐back dynamic analyses under successive earthquakes are performed for each level of existing damage. Using simulated results, failure probabilities of components for multiple limit states are computed for each bridge model and then are used to evaluate the relative vulnerability of components associated with cumulative damage and column repair. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
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The peer‐to‐peer (P2P) Internet online hybrid test system has been developed for the seismic simulation of a structure. In this study, the stability and accuracy of the system are investigated analytically by studying the spectral radius of the recursive matrix of the test scheme featuring a two‐round quasi‐Newton test scheme. The applicability of the system is further examined by exploring the seismic responses of a complex structure, a steel‐encased reinforced concrete (SRC) structure with a steel tower on the top. The structure is divided into two numerical substructures and one tested part for hybrid test. The numerical substructures are simulated by sophisticated finite element method (FEM) models with material nonlinearities to capture local plastifications. Two types of FEM programs, namely OpenSEES and ABAQUS, which are suitable for the SRC part and the steel tower, respectively, are employed. The results demonstrate that the P2P system is able to simulate complex structures with significant nonlinearities. As compared with the previous study in which two elastic numerical substructures were considered, increase in the number of iterations in this study is not significant, because the associated nonlinearities are limited due to the small time interval adopted in the test. Copyright © 2007 John Wiley & Sons, Ltd. 相似文献
66.
Post‐tensioned (PT) self‐centering moment‐resisting frames (MRFs) have recently been developed as an alternative to welded moment frames. The first generation of these systems incorporated yielding energy dissipation mechanisms, whereas more recently, PT self‐centering friction damped (SCFR) moment‐resistant connections have been proposed and experimentally validated. Although all of these systems exhibited good stiffness, strength and ductility properties and stable dissipation of energy under cyclic loading, questions concerning their ultimate response still remained and a complete design methodology to allow engineers to conceive structures using these systems was also needed. In this paper, the mechanics of SCFR frames are first described and a comprehensive design procedure that accounts for the frame behavior and the nonlinear dynamics of self‐centering frames is then elaborated. A strategy for the response of these systems at ultimate deformation stages is then proposed and detailing requirements on the beams in order to achieve this response are outlined. The proposed procedure aims to achieve designs where the interstory drifts for SCFR frames are similar to those of special steel welded moment‐resisting frames (WMRFs). Furthermore, this procedure is adapted from current seismic design practices and can be extended to any other PT self‐centering steel frame system. A six‐story building incorporating WMRFs was designed and a similar building incorporating SCFR frames were re‐designed by the proposed seismic design procedure. Time‐history analyses showed that the maximum interstory drifts and maximum floor accelerations of the SCFR frame were similar to those of the WMRF but that almost zero residual drifts were observed for the SCFR frame. The results obtained from the analyses confirmed the validity of the proposed seismic design procedure, since the peak drift values were similar to those prescribed by the seismic design codes and the SCFR frames achieved the intended performance level under both design and maximum considerable levels of seismic loading. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
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Tae‐Hyung Lee Mikiko Kato Tomohiro Matsumiya Keiichiro Suita Masayoshi Nakashima 《地震工程与结构动力学》2007,36(3):367-382
As the first part of non‐structural component test series, interior drywall partitions are selected for an experimental program. This test series will cover non‐structural components that are significant in the economic losses in buildings subjected to seismic loading, namely interior drywall partitions, exterior cladding and window glasses, and ceilings. Four full‐scale drywall partitions with light‐gage steel stud framing were tested to observe damage in cyclic loading conditions. Effects of a door and an intersecting wall on the behaviour of drywall partition are studied. Damage was concentrated to perimeter regions where gypsum boards made contacts with ceiling, floor, or columns. Dynamic loading did not amplify the damage on a drywall partition over the damage observed from the quasi‐static test. Damage–repair cost relationships show that the repair cost reaches almost the initial cost under 2% radian interstorey drift. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献
68.
Sang Yong Chung Venkatramanan Senapathi Selvam Sekar Tae Hyung Kim 《Hydrogeology Journal》2018,26(6):1875-1885
Monitoring and time-series analysis of the hydrological parameters electrical conductivity (EC), water pressure, precipitation and tide were carried out, to understand the characteristics of the parameter variations and their correlations at a coastal area in Busan, South Korea. The monitoring data were collected at a sharp interface between freshwater and saline water at the depth of 25 m below ground. Two well-logging profiles showed that seawater intrusion has largely expanded (progressed inland), and has greatly affected the groundwater quality in a coastal aquifer of tuffaceous sedimentary rock over a 9-year period. According to the time series analyses, the periodograms of the hydrological parameters present very similar trends to the power spectral densities (PSD) of the hydrological parameters. Autocorrelation functions (ACF) and partial autocorrelation functions (PACF) of the hydrological parameters were produced to evaluate their self-correlations. The ACFs of all hydrologic parameters showed very good correlation over the entire time lag, but the PACF revealed that the correlations were good only at time lag 1. Crosscorrelation functions (CCF) were used to evaluate the correlations between the hydrological parameters and the characteristics of seawater intrusion in the coastal aquifer system. The CCFs showed that EC had a close relationship with water pressure and precipitation rather than tide. The CCFs of water pressure with tide and precipitation were in inverse proportion, and the CCF of water pressure with precipitation was larger than that with tide. 相似文献
69.
Investigations undertaken in 2013 at a foreshore site that was historically used for ship repairs in Korea indicated that soil and groundwater were contaminated with petroleum hydrocarbons over an area of 10,000 m2. Groundwater investigations and modelling were undertaken to determine the characteristics of aquifers beneath the site, including the direction and rate of groundwater flow and the extent of dispersion of total petroleum hydrocarbon contamination. To identify petroleum products residing in contaminated soils, the hump of an unresolved complex mixture on the baseline found in the data of gas chromatography was used as a new method. Based on objective facts obtained from basic investigation, the current state of contamination, and discrimination of oil species, the polluter as specified in the Korean Soil Environment Conservation Act was identified. 相似文献
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