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11.
Near-complete collagen (I) sequences are proposed for elephantid and mammutid taxa, based upon available African elephant genomic data and supported with LC-MALDI-MS/MS and LC-ESI-MS/MS analyses of collagen digests from proboscidean bone. Collagen sequence coverage was investigated from several specimens of two extinct mammoths (Mammuthus trogontherii and Mammuthus primigenius), the extinct American mastodon (Mammut americanum), the extinct straight-tusked elephant (Elephas (Palaeoloxodon) antiquus) and extant Asian (Elephas maximus) and African (Loxodonta africana) elephants and compared between the two ionization techniques used. Two suspected mammoth fossils from the British Middle Pleistocene (Cromerian) deposits of the West Runton Forest Bed were analysed to investigate the potential use of peptide mass spectrometry for fossil identification. Despite the age of the fossils, sufficient peptides were obtained to identify these as elephantid, and sufficient sequence variation to discriminate elephantid and mammutid collagen (I). In-depth LC-MS analyses further failed to identify a peptide that could be used to reliably distinguish between the three genera of elephantids (Elephas, Loxodonta and Mammuthus), an observation consistent with predicted amino acid substitution rates between these species.  相似文献   
12.
This article provides information on those population education materials and services which are available from the Population Reference Bureau (PRB) and explores their usefulness in secondary school and college environments. PRB data sheets are a most useful source of information. Demographic and socioeconomic information (birth and death rates, natural increase, population doubling times, projections for the year 2000, life expectancies, per capita gross national product, etc.) are included. The World's Women Data Sheet is a special publication issued to mark the midway point of the UN Decade for Women. 161 countries are included with information on demographic, health, employment, and educational status of women. There is a nominal cost for these data sheets. To help students learn about population dynamics and world patterns of population, mapping exercises can be developed. The Population Bulletin is published 6 times/year and each issue concentrates on specific aspects of population. The 28 bulletins which have been published since January 1976 are listed. The PRB Chart Series is a multicolor graphic presentation of topical issues in population and development trends worldwide. A total of 26 charts in 4 series have been produced and cover such topics as world population growth, urbanization, world energy consumption, age structure in the U.S., and adolescent pregnancy in the U.S. This material is best used with high school students. A monthly international population news magazine called Intercom is desinged to inform about worldwide trends and activities in population and family planning. It is published in both English and Spanish and is a useful source of current information in capsulized form. For population educators there is the bimonthly newsletter Interchange. PRB's teaching-learning modules are valuable publications for teachers. Thus far there are 9, each of which is a self-contained unit with classroom activities for junior high to college students. Each one outlines a problem and then presents methods which can stimulate discussion and provoke interest. 2 special publications on special interest topics (Population Handbook; Population Education: Sources and Resources) are described. PRB may well be the best single source of population education materials which will hopefully be used and incorporated into teaching.  相似文献   
13.
A variety of anthropogenic chemicals are capable of binding to the estrogen receptor of vertebrate species. Binding of these compounds can interfere with homeostasis by disrupting normal gene expression patterns. The purpose of this study was to investigate the feasibility of applying array technology as a monitoring tool for detecting the presence and distribution of estrogenic compounds in coastal habitats using sheepshead minnows as our model. cDNA clones that were isolated from differential display, including vitellogenin alpha and beta, vitelline envelope protein (ZP2), and transferrin, among others, were spotted on the macroarray. The results of these experiments demonstrate a characteristic expression pattern of estrogen responsive genes in sheepshead minnows exposed to 17 beta-estradiol (E2).  相似文献   
14.
In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N1-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted.  相似文献   
15.
The Paris Agreement, which entered into force in 2016, sets the ambitious climate change mitigation goal of limiting the global temperature increase to below 2°C and ideally 1.5°C. This puts a severe constraint on the remaining global GHG emissions budget. While international shipping is also a contributor to anthropogenic GHG emissions, and CO2 in particular, it is not included in the Paris Agreement. This article discusses how a share of a global CO2 budget over the twenty-first century could be apportioned to international shipping, and, using a range of future trade scenarios, explores the requisite cuts to the CO2 intensity of shipping. The results demonstrate that, under a wide range of assumptions, existing short-term levers of efficiency must be urgently exploited to achieve mitigation commensurate with that required from the rest of the economy, with virtually full decarbonization of international shipping required as early as before mid-century.

Key policy insights

  • Regulatory action is key to ensuring the international shipping sector’s long-term sustainability.

  • For the shipping industry to deliver mitigation in line with the Paris Agreement, virtually full decarbonization needs to be achieved.

  • In the near term, immediate and rapid exploitation of available mitigation measures is of critical importance.

  • Any delay in the transition will increase the risk of stranded assets, or diminish the chances of meeting the Paris Agreement's temperature commitments.

  相似文献   
16.
Coral reef ecosystems, the most varied on earth, continually face destruction from anthropogenic and natural threats. The U.S. Coral Reef Task Force seeks to characterize and map priority coral reef ecosystems in the U.S./Trust Territories by 2009. Building upon NOAA Biogeography shallow-water classifications based on Ikonos imagery, presented here are new methods, based on acoustic data, for classifying benthic terrain below 30 m, around Tutuila, American Samoa. The result is a new classification scheme for American Samoa that extends and improves the NOAA Biogeography scheme, which, although developed for Pacific island nations and territories, is only applicable to a maximum depth of 30 m, due to the limitations of satellite imagery. The scheme may be suitable for developing habitat maps pinpointing high biodiversity around coral reefs throughout the western Pacific.  相似文献   
17.
Moderate-resolution, near-IR spectroscopy of MS 1512-cB58 is presented, obtained during commissioning of the near-infrared spectrometer (NIRSPEC) on the Keck II telescope. The strong lensing of this z=2.72 galaxy by the foreground cluster MS 1512+36 makes it the best candidate for detailed study of the rest-frame optical properties of Lyman-break galaxies. In 80 minutes of on-source integration, we have detected Halpha, [N ii] lambdalambda6583, 6548, [O i] lambda6300, He i lambda5876, [O iii] lambdalambda5007, 4959, Hbeta, Hgamma, [O ii] lambda3727, and a strong continuum signal in the range of 1.29-2.46 μm. A redshift of z=2.7290+/-0.0007 is inferred from the emission lines, in contrast to the z=2.7233 calculated from UV observations of interstellar absorption lines. Using the Balmer line ratios, we find an extinction of E(B-V) = 0.27. Using the line strengths, we infer a star formation rate (SFR) of 620+/-18 M middle dot in circle yr-1 (H0=75, q0=0.1, and Lambda=0), which is a factor of 2 higher than that measured from narrowband imaging observations of the galaxy but is a factor of almost 4 lower than the SFR inferred from the UV continuum luminosity. The width of the Balmer lines yields a mass of Mvir=1.2x1010 M middle dot in circle. We find that the oxygen abundance is 13 solar, in good agreement with other estimates of the metallicity. However, we infer a high nitrogen abundance, which may argue for the presence of an older stellar population.  相似文献   
18.
A number of investigations in the recent decades have shown that footing uplift can reduce the seismic loading on a structure. Guidelines to design a structure with seismic uplift capability have been proposed. However, these studies mainly focus on the structural response and neglect the impact forces on the footing from re‐contact between the footing and the supporting medium. A small number of computational studies of the induced forces on the footing have been performed. This paper presents the results of free vibrations and shake table tests on a single degree‐of‐freedom model of a bridge pier with footing uplift on a rigid base. Two support conditions are considered, that is, footing fixed to the base and footing free to uplift on a rigid base. Load cells were placed at the interface of the footing and rigid base to measure the contact forces during structural vibration. The footing responses of both flexible and rigid structures due to free vibration are compared. The results show that the flexibility of the structure has significant effects on footing uplift duration and amplitude and reduces the contact force, in some cases very significantly. The flexible structure was also subjected to harmonic base excitations. It is found that varying the characteristics of the excitation changes the uplift amplitude but does not affect the contact force significantly. Copyright © 2017 John Wiley & Sons, Ltd.  相似文献   
19.
Often facilitated by human-mediated pathways, aquatic invasive species are a threat to the health and biodiversity of global ecosystems. We present a novel approach incorporating survey data of watercraft movement in a social network analysis to reconstruct potential pathways of aquatic invasive species spread between lakes. As an example, we use the green alga Nitellopsis obtusa, also known as starry stonewort, an aquatic invasive species affecting the Great Lakes region in the United States and Canada. The movement of algal fragments via human-mediated pathways(i.e., watercraft) has been hypothesized as the primary driver of starry stonewort invasion. We used survey data collected at boat ramps during the 2013 and 2014 openwater seasons to describe the flow of watercraft from Lake Koronis, where N. obtusa was first detected in Minnesota, to other lakes in the state. Our results suggest that the risk of N. obtusa expansion is not highly constrained by geographic proximity and management efforts should consider highly connected lakes.Estimating human movement via network analysis may help to explain past and future routes of aquatic invasive species infestation between lakes and can improve evidence-based prevention and control efforts.  相似文献   
20.
北山柳园地区中志留世埃达克质花岗岩类及其地质意义   总被引:7,自引:3,他引:4  
北山柳园地区发育的埃达克质片麻状花岗闪长岩为钙碱性岩浆系列,具有较高SiO2 (>56%),Al2O3 (>15%)和较低的MgO (<3%)含量,Na2O>K2O; 并且具有高的Sr含量(>400×10-6)和Sr/Y比值; 样品轻重稀土强烈分异(La/Yb)N =18~86,强烈亏损重稀土Yb与Y,具有不明显的Eu异常(δEu=0.90~0.95); 富集LREE和大离子亲石元素(LILE),而亏损HREE、高场强元素(HFSE: Nb、Ta),与世界上典型的俯冲洋壳熔融形成的埃达克岩相似。然而样品具有相对高的(87Sr/86Sr)I (0.70635~0.70636)和相对低的εNd(t) (-0.8~-0.9),以及锆石具有相对较低的εHf (t) (-0.8~+2.7)同位素特征,比典型的俯冲洋壳熔融形成埃达克岩具有更多的放射成因,推测可能是源区加入了地壳物质/沉积物/或特殊的洋壳(OIB/E-MORB)熔融,以及侵位过程中地壳物质的混染所造成的。埃达克质片麻状黑云母花岗岩锆石LA-ICPMS年龄为424±4Ma,代表了花岗岩埃达克花岗岩的结晶年龄。花牛山岛弧带在中晚志留世时期具有较高的地热梯度,发育了大面积高εNd(t)钙碱性花岗岩和区域围岩发生了高温变质作用。因此,柳园埃达克岩是由于热的洋壳向花牛山岛弧地体俯冲过程中熔融形成的,俯冲洋壳熔融是本地区早古生代大规模地壳增生的重要方式之一。  相似文献   
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