Characterization of EmpA protease in Vibrio anguillarum M3     

Yifan Han  Zhaolan Mo  Peng Xiao  Bin Hao  Jie Li  Guanpin Yang 《中国海洋大学学报(英文版)》2011,10(4):379-384

EmpA is an extracellular metalloprotease secreted by Vibrio anguillarum.For better understanding its role in the patho-genicity of V.anguillarum strain M3,empA insertion mutant was constructed.In the mutant it decreased in extracellular proteolytic activity,swarming motility,hemolytic activity and virulence on turbot(Scophthalmus maximus).Significant decline(by 5-fold)of extracellular proteolytic activity and similar growth curve between mutant and wild type strains indicated that EmpA was the major extracellular protease of M3.LD50 of mutant increased by 38-fold compared with wild type.No pro-EmpA was detected in the su-pernatant of culture,indicating that EmpA autolyzed to mature protein after 24 h.Secretion of EmpA in M3 was similar to that in NB10 strain.Attenuated virulence of mutant was similar to that of M93Sm strain.It was demonstrated that specific operation of EmpA was different from that in previous studies and EmpA contributed to the swarming motility and hemolytic activity in V.an-guillarum strain M3.The results provides insight into understanding the function of EmpA and its potential application in vaccine development.  相似文献   

阿克苏河流域气候变化对潜在蒸散量影响评价(英文)   总被引：1，自引：1，他引：0  

张守红  刘苏峡  莫兴国  舒畅  孙杨  张春 《地理学报(英文版)》2011,21(4):609-620

Evapotranspiration is one of the key components of hydrological processes. Assessing the impact of climate factors on evapotranspiration is helpful in understanding the impact of climate change on hydrological processes. In this paper, based on the daily meteorological data from 1960 to 2007 within and around the Aksu River Basin, reference evapotranspiration (RET) was estimated with the FAO Penman-Monteith method. The temporal and spatial variations of RET were analyzed by using ARCGIS and Mann-Kendall method. Multiple Regression Analysis was employed to attribute the effects of the variations of air temperature, solar radiation, relative humidity, vapour pressure and wind speed on RET. The results showed that average annual RET in the eastern plain area of the Aksu River Basin was about 1100 mm, which was nearly twice as much as that in the western mountainous area. The trend of annual RET had significant spatial variability. Annual RET was reduced significantly in the southeastern oasis area and southwestern plain area and increased slightly in the mountain areas. The amplitude of the change of RET reached the highest in summer, contributing most of the annual change of RET. Except in some high elevation areas where relative humidity predominated the change of the RET, the variations of wind velocity predominated the changes of RET almost throughout the basin. Taking Kuqa and Ulugqat stations as an example, the variations of wind velocity accounted for more than 50% of the changes of RET.  相似文献   

页岩气测井地层评价的方法与进展   总被引：1，自引：0，他引：1  

莫修文  李舟波  潘保芝 《地质通报》2011,30(2)

页岩气测井地层评价主要围绕3个方面展开:①页岩气地层的岩性和储集参数评价,包括孔隙度、饱和度、渗透率等;②页岩的生烃潜力评价,主要包括干酪根的识别与类型划分、有机质含量、热成熟度等一系列指标的定性或定量解释;③岩石力学参数和裂缝发育指标的评价.特别是在资源调查和勘探的初期,如何从烃源岩中寻找最有利的页岩气藏富集,是地球物理测井的首要任务.近几年,国外在页岩气测井评价方面的工作主要体现在对ECS测井和高分辨率成像测井的应用.另外有研究表明,感应测井与岩石介电常数有关的X分量负异常与干酪根的存在及其类型之间有一定的相关性.  相似文献   

Tracing origins of sewage and organic matter using dissolved sterols in Masan and Haengam Bay,Korea     

Hyo Jin Lee  Sang Hee Hong  Moonkoo Kim  Sung Yong Ha  Soon Mo An  Won Joon Shim 《Ocean Science Journal》2011,46(2):95-103

Masan and Haengam Bays in Korea are highly polluted and semi-enclosed. Domestic and industrial effluents are directly or indirectly discharged into the bays through sewage treatment plants (STP) and creeks. In this study, 15 dissolved sterol compounds were determined in order to understand their sources and relative contribution. Freshwater samples were taken from 13 creeks and at two STP sites on a monthly basis. Total dissolved sterol concentrations ranged from 993 to 4158 ng/L. The concentrations of sterols in winter were higher than in summer. Among the sterols analyzed, cholesterol, β-sitosterol, coprostanol and cholestanone were major compounds in creek water. Seawater samples were concurrently collected at 21 stations in Masan Bay. Total sterol concentrations ranged 118–6,956 ng/L. Inner bay showed high concentrations of sterols in summer, while outer bay showed high sterol concentrations in winter. Among the sterols, cholesterol, β-sitosterol and brassicasterol were major compounds in seawater. In order to examine the contribution of urban sewage, the concentration of coprostanol and fecal sterol ratios were calculated. Most of the creek water, inner bay and near STP outlet samples were affected by sewage. Terrestrial organic matters accounted for a high proportion of dissolved organic matter origin. Fecal origins were relatively high in the inner bay areas and in the STP outlet, while sterols of marine origin were high in the outer bay areas.  相似文献   

海洋底质测量专用标准规范研究          下载免费PDF全文

崔高嵩  金绍华  王沫  刘涛 《海洋测绘》2011,31(4):39-42

研究海洋底质测量专用标准体系构成及作业规范技术要点.分析专用海洋底质测量的任务构成和技术特点,依据底质测量的专用需求和国内外底质测量技术规范与数据格式标准的发展趋势,对海洋底质测量专用标准体系构成、数据格式标准及作业技术规程进行了探讨.  相似文献   

Implications of trace element composition of syntaxial quartz cements for the geochemical conditions during quartz precipitation in sandstones     

Thomas Götte  Karl Ramseyer  Thomas Pettke  Monika Koch‐Müller 《Sedimentology》2013,60(5):1111-1127

A petrographic investigation revealed polyphase quartz cementation in the Finefrau Sandstone (Upper Carboniferous, Western Germany) and the Solling Sandstone (Lower Triassic, Central Germany). Three different cements could be distinguished in each sandstone based on their cathodoluminescence and trace element composition. The first quartz generation is suggested to have been formed during eogenesis due to dissolution and replacement of feldspar. The mesogenetic paragenesis comprises two generations of quartz and illite, which are accompanied by albite in the Solling Sandstone. Sharp luminescence zoning in quartz overgrowths points to distinct episodes of cementation in both sandstones. Significant amounts of Al, Li and H and traces of Ge and B have been detected in the quartz overgrowths. The Al‐content of the quartz cements in the Finefrau Sandstones exceeds that in the quartz cements in the Solling Sandstone by a factor of five. It is suggested that this compositional variation reflects the conditions in the pore‐water, such as temperature and pH. The Al‐concentration is generally correlated to the Li‐content with the exception of the latest quartz generation in the Finefrau Sandstones which is also most enriched in trace elements. The ratio of Li/Al varies between 0·11 and 0·25 in the two sandstones. The Li/H‐ratio, which ranges from 0·12 to 0·3, is controlled by the activity ratio of Li and H in the pore fluid. Clay minerals are the most important source for Li and high salinities favour the mobilization of Li during diagenesis. Thus, a relatively low salinity and low pH are responsible for the low Li/H‐ratio in the Finefrau Sandstone, while high salinity and neutral to alkaline pH results in a high Li/H‐ratio for the Solling Sandstone. The Ge‐contents are generally near the average of detrital quartz and indicate that pressure dissolution is a major source for quartz cementation. Different chemical compositions of distinct quartz generations indicate changes in the physico‐chemical conditions and point to mobilization of silica from different sources (for example, pressure solution and clay mineral transformations).  相似文献   

甘肃拾金坡金矿床成因:来自⁴⁰Ar/³⁹Ar定年、成矿流体及H-O-S同位素证据     

朱江  吕新彪  莫亚龙  曹晓峰  陈超 《吉林大学学报(地球科学版)》2013,(2):427-439

甘肃拾金坡金矿床是北山造山带南部的一处重要中型金矿床。矿体以石英脉和蚀变岩形式产出,受东西向断裂控制,赋矿围岩为早泥盆世拾金坡二长花岗岩。矿石中主要金属硫化物为黄铁矿及少量方铅矿、闪锌矿和磁黄铁矿。石英脉两侧花岗岩发生强烈的硅化、黄铁绢英岩化和绿泥石化。热液蚀变绢云母40 Ar/39 Ar坪年龄为(364.6±3)Ma,表明成矿作用发生在晚泥盆世。矿石石英中流体包裹体可分为早晚两期:早期流体包裹体群包含不同气液比水溶液包裹体和含CO2包裹体,指示成矿过程存在流体沸腾作用,其均一温度集中在280～325℃,盐度(w(NaCl))为7.86%～9.21%;晚期流体包裹体群只发育水溶液包裹体,均一温度集中于160～230℃,盐度为9.47%～11.10%。成矿流体属中温低盐度、富CO2流体。流体从早阶段演化到晚阶段,温度降低约100℃,压力由约113 MPa骤减到约11 MPa。流体包裹体的δ18 OH2O值为1.39‰～3.39‰,δD值为-71‰～-99‰。氢氧投图中,石英脉和蚀变岩样品构成良好的线性关系,暗示初始成矿流体主要来自变质热液,晚期有强烈大气降水加入。矿石金属硫化物δ34S值变化范围为4.44‰～11.33‰,峰值为8‰～9‰,呈塔式分布。与区域S同位素资料对比分析表明,石英脉型矿石S主要来自前寒武系基底,蚀变岩型矿石S更多地继承了花岗岩围岩S。综合分析认为,拾金坡金矿床属于造山型金矿,形成于造山晚期挤压—伸展转换环境。  相似文献   

关于意愿调查价值评估法在我国环境领域应用的可行性探讨——以北京市居民支付意愿研究为例   总被引：49，自引：3，他引：49  

杨开忠  白墨  李莹  薛领  王学军 《地球科学进展》2002,17(3):420-425

从意愿调查价值评估法的优越性和局限性出发，以在北京市进行的关于市民为改善大气环境质量的支付意愿的调查研究为例，详细分析了调查方法和结果。通过对结果的评价，探讨了意愿调查价值评估法在我国进行环境物品价值评估实践中的可行性，得出了这一方法在我国具有推广前景的结论。最后对意愿调查价值评估法今后在我国实践中的应用提出了几点建议。  相似文献   

无锡光伏产业链中的全球-本地联系   总被引：4，自引：1，他引：4  

童昕  王涛  李沫 《地理科学》2017,37(12):1823-1830

以无锡市为例,通过实地调研当地光伏产业链中多晶硅生产、电池元件生产、组件制造、相关设备生产、光伏设备运维服务,以及光伏用户等不同环节,从全球生产网络的视角,考察全球-本地联系在当地光伏产业链形成演化和技术发展动态中的具体表现。结论指出：① 无锡光伏产业在短短10 a之内从“两头在外”的发展模式到形成全产业链竞争优势,体现了新兴技术的全球生产网络技术加速更新和产业快速转移的新特点;② 本地相关产业集群通过技术引进、消化和改进为新技术突破规模化瓶颈,获取市场竞争力打下基础,并由此对全球光伏技术发展的路径产生了关键影响;③ 产业进一步发展需要针对能源转型的长期目标,着重探索能源消费侧的技术转型路径。  相似文献   

Flux and dispersion of gases from the “Drachenschlund” hydrothermal vent at 8°18' S, 13°30' W on the Mid-Atlantic Ridge     

Robin S. Keir  Oliver Schmale  Maren Walter  Jürgen Sültenfuß  Richard Seifert  Monika Rhein 《Earth and Planetary Science Letters》2008,270(3-4):338-348

Hydrothermal emission of mantle helium appears to be directly related to magma production rate, but other processes can generate methane and hydrogen on mid-ocean ridges. In an on-going effort to characterize these processes in the South Atlantic, the flux and distribution of these gases were investigated in the vicinity of a powerful black smoker recently discovered at 8°17.9' S, 13°30.4' W. The vent lies on the shoulder of an oblique offset in the Mid-Atlantic Ridge and discharges high concentrations of methane and hydrogen. Measurements during expeditions in 2004 and 2006 show that the ratio of CH₄ to ³He in the neutrally buoyant plume is quite high, 4 × 10⁸. The CTD stations were accompanied by velocity measurements with lowered acoustic Doppler current profilers (LADCP), and from these data we estimate the methane transport to have been 0.5 mol s^− 1 in a WSW-trending plume that seems to develop during the ebb tidal phase. This transport is an order of magnitude greater than the source of CH₄ calculated from its concentration in the vent fluid and the rise height of the plume. From this range of methane fluxes, the source of ³He is estimated to be between 0.14 and 1.2 nmol s^− 1. In either case, the ³He source is significantly lower than expected from the spreading rate of the Mid-Atlantic Ridge. From the inventory of methane in the rift valley adjacent to the vent, it appears that the average specific rate of oxidation is 2.6 to 23 yr^− 1, corresponding to a turnover time between 140 and 16 days. Vertical profiles of methane in the surrounding region often exhibited Gaussian-like distributions, and the variances appear to increase with distance from the vent. Using a Gaussian plume model, we obtained a range of vertical eddy diffusivities between 0.009 and 0.08 m²m² s^− 1. These high values may be due to tidally driven internal waves across the promontory on which the vent is located.  相似文献