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Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome 总被引：1，自引：0，他引：1
An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified
and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing
four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene.
Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309) 相似文献
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水产动物遗传连锁图谱的构建策略 总被引：1，自引：0，他引：1
遗传连锁图谱的构建是实现对水产动物进行数量性状基因定位的基础,同时也是运用分子标记实现基因辅助育种的有效工具.通过评价不同分子标记作图的优缺点,介绍水产动物遗传连锁图谱构建使用的分离群体,提出了适合水产动物遗传图谱构建的有效方法. 相似文献
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This pathogenic study shows that the viral diseases of Chinese prawns (Penaeus chinensis. O'sbeck)is due to three kinds of viruses: epithelium envelope baculovirus of Penaeus chinensis (EEBV-PC, de-tected by the authors in 1993), infections hypodermal and hematopoietic necrosis virus, andhepatopancreatic parvo-like virus, and that the first two viruses seem to be the main pathogens of theepidemic in the northern regions in 1993. 相似文献
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This paper describes a rapid procedure for diagnosis of penaeid viral disease using the immune serological method. The SPA (Staphylococcal Protein A) coagglutination test in early diagnosis of penaeid viral disease has yielded satisfactory results.This is the first time SPA is used in China for diagnoses of viral diseases of marine invertebrates, especially penaeid shrimp.The SPA early diagnostic method is characterized by accuracy, rapidity, simplicity and convenience, low cost, high specificity, strong sensitivity and micro-detectability, and easy dissemination and adaptability in prawn farms. 相似文献
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初期成活率是对虾养殖的第一个关键问题，如果初期虾池环境不良，虾苗会大量死亡。本文探索了优化虾苗投放环境的方法并提出了营养盐的合理指标。研究结果表明，良好的虾苗投放生态环境对于整个养成期十分重要。１材料与方法本试验在河北省唐海县十里海养殖场进行。试验面积１３０００亩，试验时间是１９９０～１９９１年，设对照池３０００亩，试验对象为养殖的中国对虾。探索适当的肥水方法、监测浮游生物量、水质、虾苗成活率和旬生长速度等，推定提高初期成活率的虾池环境指标。２结果与讨论１９９０年春，虾苗入地后一个月生长十分缓慢… 相似文献
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于1987—1991年,在河北省唐海县十里海养殖场进行了中国对虾大面积集约化养殖高产技术研究,内容报告于下。 相似文献
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1977年10—11月和1978年9—10月,在东海陆架及邻近海区(26°00'—34°00'N,124°00'—129°00'E,水深39—1550m)进行底栖生物定量调查,全海区共布设定量采泥307站,获得底栖生物定量标本1200号。调查海区底栖生物的生物量较低,总生物量平均为11.5g/m2,栖息密度为30个/m2,生物量组成以棘皮动物占优势(5.4g/m2),依次为软体动物(2.6g/m2),多毛类(1.7g/m2),甲壳动物(0.5g/m2),生物量分布呈现随着深度的增大而减小的趋势,以水深50—100m为最高,生物量以粉砂底质最高(14.3g/m2),中细砂和细砂底质较接近(10g/m2左右),软泥底质最低,仅4.0g/m2。 相似文献