大菱鲆(Scophthalmus maximus)耐高温品系选育及耐温性能评估   总被引：1，自引：1，他引：0  

马爱军  黄智慧  王新安  郭黎  雷霁霖  杨志  曲江波 《海洋与湖沼》2012,43(4):797-804

采用家系选育方法,进行了大菱鲆耐高温性状选育研究,对两代家系耐温性能进行测定,并利用bCOX回归分析了各家系的耐温性优势比。结果表明:各家系耐温性差异达到了显著水平(P<0.05),并且耐高温能力的优势比也存在显著差异,这反映出在大菱鲆耐高温选育中家系选择的高效性;经过一代选育,获得4个耐高温家系,并据此构建选育二代家系。耐温性能评估,选育二代比一代有显著提高,在27℃高温条件下,选育一代的平均死亡率为49%,选育二代的平均死亡率为10.26%;28℃条件下,选育二代耐高温子代的平均死亡率为32.83%,对照组的平均死亡率为88.9%;经过筛选后,选育二代的耐温性比选育一代提高了1—2℃左右。  相似文献   

Morphological comparison between a selected fast-growing strain and the common cultured strain of turbot Scophthalmus maximus   总被引：2，自引：0，他引：2  

梁兴明  马爱军  王新安  李娟  黄智慧  商晓梅 《中国海洋湖沼学报》2012,30(4):563-568

To evaluate the effect of genetic improvement of the turbot Scophthalmus maximus, we analyzed morphological differences between a fast-growing strain obtained by family selection and the common cultured strain, by principal component analysis, stepwise discriminant analysis, and t-tests. Although they clearly differed morphologically, plots of the principal components of the two strains partially overlapped. However, the difference between the strains was supported with very high precision by discriminant analysis. The t-tests revealed that 4 of the 13 morphological traits analyzed were highly significantly different (P<0.01), 4 traits also differed significantly (P<0.05), and the remainder did not differ significantly. The coefficients of difference of the 13 traits were all lower than the threshold value between subspecies (1.28). Together, the results indicate that a trend for segregation of characters from the common cultured strain have already appeared in the selected fast-growing strain but the degree of segregation have not risen to subspecies level.  相似文献   

氨对真鲷幼鱼生长的危害     

马爱军  雷霁霖  高淳仁 《海洋科学》2000,24(1):14-15

IMPERILMENTOFAMMONIAONTHEGROWTHOFYOUNGPagrosomusmajor氨对养殖鱼类的毒性,已经引起很多关注,特别随着鱼类养殖的发展。氨浓度的增加成为影响集约化养殖。尤其是影响增加放养密度的主要问题。关于非离子形态的氨对鱼类和水生生物的毒害作用,国内外已有许多报道,如Robinette1976年,雷衍之和金送笛1979年,周永欣1986年．Chen1992年等。Colt和Armstrong1981年整理了氮化合物对水生生物影响后指出,非离子氨的主要效应为亚致死影响,而生长缓慢是一种最重要的亚致死影响。由于不同作者的实验对象种类不同,以及同种…  相似文献   

中国对虾幼体中肠的超微结构   总被引：7，自引：0，他引：7  

张志峰  马爱军  于利  廖承义 《海洋与湖沼》1999,30(2):145-149

１９９５年４月,在山东日照市涛雒对虾育苗场采集中国对虾各期幼体,采用超薄切片和电观察的方法,对其中肠结构进行观察研究,结果表明,中国对虾幼体的中肠肠壁由３部分组成,即肠上皮、基膜和肌层。中肠上皮细胞具发达的微绒毛,胞质中富含线粒体、内质网、高尔基复合体及酶原颗粒等,它由两类细胞组成。第一类细胞在发育早期占多数,随着幼体的发育逐渐减少,至仔虾期后,肠上波完全由第二类细胞组成,基膜分为内、外两层,且在  相似文献   

大菱鲆(Scophthalmus maximus)蛋白质二硫键异构酶SmPDIA3的表达分析和功能验证     

唐启政  孙志宾  王新安  马爱军  杨双双  黄智慧 《海洋与湖沼》2019,50(2):409-419

本研究利用SMART-RACE技术克隆了大菱鲆SmPDIA3基因。SmPDIA3基因cDNA序列全长2083bp，包括1479bp的开放阅读框，编码492个氨基酸，GenBank登录号：MG765516。组织表达分析发现：SmPDIA3基因在肠、鳃、肝脏等组织中均有表达，其中在肝脏中表达量最高，脑中最低。进一步研究了温度胁迫后SmPDIA3基因在肠和肝脏组织中的表达变化规律，发现随着胁迫温度的升高，SmPDIA3基因的相对表达量总体上升，并在28℃时达到最大。利用原核表达技术，构建了pET-28a-PDIA3原核表达载体，IPTG诱导后融合蛋白主要在上清中表达。将上清中的蛋白分离纯化后，利用Western blot技术验证了目的蛋白的准确性。将纯化后的目的蛋白浓缩后，利用BCA蛋白浓度测定试剂盒测得蛋白浓度为243.18μg/mL。最后设计变性溶菌酶的复性实验，验证了SmPDIA3蛋白具有分子伴侣功能，能够辅助变性蛋白的正确折叠。  相似文献   

Interaction of temperature and salinity on the expression of immunity factors in different tissues of juvenile turbot Scophthalmus maximus based on response surface methodology     

黄智慧  马爱军  王新安  雷霁霖  李伟业  王婷  杨志  曲江波 《中国海洋湖沼学报》2015,33(1):28-36

Central Composite Design(CCD) and response surface methodology were used in the experiment to examine the combined effect of temperature(16-28℃) and salinity(18-42) on Hsp70 and IgM genes expression levels in turbot(Scophthalmus maximus) liver and kidney.The results showed that the coefficients of determination(R~2=0.965 2 for liver Hsp70,0.972 9 for kidney Hsp70,0.921 for liver IgM and 0.962 1 for kidney IgM) and probability values(P0.01) were significant for the regression model.The interactive effect between temperature and salinity on liver Hsp70,kidney Hsp70 and liver IgM were not significant(P0.05),while the interactive effect between temperature and salinity on kidney IgM was significant(P0.01).The model equation could be used in practice for forecasting Hsp70 and IgM genes expression levels in the liver and kidney of juvenile turbot via applying statistical optimization of the response of interest,at which the maximum liver Hsp70,kidney Hsp70,liver IgM and kidney IgM of1.48,1.49,2.48,and 1.38,respectively,were reached.The present model may be valuable in assessing the feasibility of turbot farming at different geographic locations and,furthermore,could be a useful reference for scientists studying the immunity of turbot.  相似文献   

Comparison of the morphometric dynamics of fast-growing and slow-growing strains of turbot Scophthalmus maximus     

王新安  马爱军 《中国海洋湖沼学报》2015,33(4):890-894

The dynamics of changes in body shape of fast-growing and slow-growing strains of turbot Scophthalmus maximus, and of the differences in body shape between the two strains, were evaluated from 3 to 27 months of age. The ratios of total length/body length, body width/body length and total length/body width were used as morphometric indices. The two strains exhibited different temporal trends in total length/body length but similar trends in body width/body length and total length/body width. Generally, body width/body length of the two strains increased with time and total length/body width decreased. Thus, the bodies of both fast-growing and slow-growing strains of turbot changed from a narrow to a more rounded shape. However, the ratio total length/body length was generally lower, body width/body length was mostly higher and total length/body width was consistently lower in the fast-growing strain than in the slowgrowing strain. Correlation analysis of the three shape ratios with body weight showed that total length/body length and total length/body width were unsuitable, and that width/body length was suitable, for use as a phenotypic marker for selective breeding of turbot for growth in weight.  相似文献   

大菱鲆与耐高温性状相关的微卫星标记筛选   总被引：2，自引：0，他引：2  

马爱军  许可  黄智慧  王新安 《海洋科学进展》2011,29(3)

采用微卫星分子标记(SSR)技术分析大菱鲆(Scophthalmus maximus L.)耐高温相关特性,为耐高温大菱鲆的分子辅助育种提供合适的分子标记.将大菱鲆经过高温实验处理,区分为耐高温组与高温敏感组,用于实验分析.采用Salah.M法抽提大菱鲆肌肉组织的DNA,根据已知的30个大菱鲆微卫星位点的侧翼保守序列设计引物,进行微卫星引物PCR(SSR-PCR)扩增.对PCR扩增出的差异条带进行个体统计,最后进行微卫星位点与耐高温性状的相关性分析.结果表明,有1个微卫星位点与大菱鲆耐高温性状存在一定的负相关性;有3个微卫星位点与大菱鲆耐高温性状存在正相关性,其中位点Sma-USC27 286 bp的等位基因片段与耐高温性状的正相关性极显著,相关系数达到0.383(P＜0.01),其余2个位点为一般显著性相关.微卫星位点Sma-USC27所扩增出的差异等位基因片段可作为分子标记指导耐高温大菱鲆的辅助育种.  相似文献   

黔阳地区农业地质背景与农业优势区分析     

马爱军  彭学军  曾钦旺 《国土资源导刊(湖南)》2002,21(2):154-159

为了扩大区域地质调查成果的服务领域,通过地层岩石、地质构造、地貌、水文地质、地球化学背景及气候等因素的综合分析,结合农、林、果、药作物及畜牧渔业的生产状况.将黔阳地区划分为橙桔柚、优质稻、林、林桔橙、板栗林、桔橙林、林茶7个优势区.对合理调整农业生产结构,建设名优特农产品基地等具有重要作用.  相似文献   

眼斑双锯鱼(Amphiprion ocellaris)发育中体色花纹时序发生的色素细胞变化和控制基因表达的分析I.胚胎时期     

孙伟恒  孙志宾  王新安  马爱军  李迎娣  苟冬惠  于宏  李昊喆  VorathepMuthuwan  曲江波  洪宜展 《海洋与湖沼》2024,55(2):489-498

眼斑双锯鱼(Amphiprion ocellaris)属于鲈形目、雀鲷科、双锯鱼属, 是热带珊瑚礁观赏鱼类的首选品种。不同体色和花纹与色素细胞相关基因的表达直接相关, 在其胚胎发育的过程中, 色素细胞的形态和数量一直在变化, 但不同发育时期各种色素细胞的动态变化及其控制基因表达情况等仍需明确研究。记录了眼斑双锯鱼胚胎时期色素细胞变化较为明显的6个发育时期, 观察了不同时期的体色及色素细胞的变化特点, 并利用荧光定量PCR检测了各发育时期的10个体色控制基因的表达情况。结果显示: 在胚胎发育阶段, 整个胚胎的颜色从橙黄逐渐转变为暗红、黑红、黑色到最终透明, 观察到卵黄囊表面幼体黑色素细胞到成体黑色素细胞的转变过程, 鱼体表面在翻转期出现成体黑色素细胞、在器官形成期出现红色素细胞, 眼睛及腹部在孵化期出现虹彩色素细胞。结合荧光定量PCR结果分析发现: 卵黄囊表面幼体黑色素细胞推测是外胚层中的神经嵴细胞直接发育而成, 不需要迁移过程; Ednrb、TYR、Tbx2b基因对幼体黑色素细胞的形成和发育有重要作用, Pax3、Dct、Aim1基因与成体黑色素细胞的分化、迁移、形成过程密切相关; 其中TYR基因的相对表达量在体节期出现显著增加, 说明TYR基因在胚胎发育初期就参与黑色素细胞的形成; Tbx2b基因不仅对黑色素细胞形成有影响, 对眼斑双锯鱼心脏的形成也有一定作用; Fms基因对红色素细胞形成有重要作用; Ltk基因的表达要早于虹彩色素细胞的出现; 相关性分析发现各基因之间存在相互作用。  相似文献