培养基对海藻真菌PT2菌株的生长及活性代谢产物合成的影响   总被引：3，自引：0，他引：3  

彭琛  郑忠辉  杜希萍  黄耀坚  宋思扬  苏文金 《台湾海峡》2005,24(1):90-96

本文采用PD、GPY、YES、SYE、GPM5种不同培养基培养海藻真菌菌株PT2，并对其在不同培养基中的生长情况、代谢产物的形成及抗肿瘤活性进行分析，旨在了解不同培养基对菌株生长和活性物质合成的影响．结果显示，PT2菌株在不同培养基上的培养特征及生长速率有所不同，液体培养基发酵液抽提物的抗肿瘤活性也有较大差别，其中SYE发酵液抽提物活性最强，对KB、Raji和Hela 3种肿瘤细胞的IC50分别为7．6μg／cm^3、2．4μg／cm^3和3．3μg／cm^3．采用HPLC法对不同抽提物进行分析，发现它们之间的组成成分有显著差异．本文的实验结果表明，不同培养基对该菌株的生长及活性代谢产物的合成具有显著的影响。  相似文献   

Spatial and temporal variability of heat content above the thermocline in the tropical Pacific Ocean   总被引：1，自引：0，他引：1  

Pu Shuzhen  Yu Fei  Hu Xiaomin  Chen Xingrong 《海洋学报(英文版)》2003,22(2):179-190

Abstract-Heat content of the upper layer above the 20℃ isotherm in the tropical Pacific Ocean isestimated by using the sea temperature data set with a resolution 2°latitude×5°longitude (1980～1993)for the water depths (every 10 m) from 0 m to 400 m, and its temporal and spatial variabilities are an-alyzed. (1) The temporal variability indicates that the total heat in the upper layer of the equatorial Pa-cific Ocean is charcterized by the interannual variability. The time series of the equatorial heat anomaly5 months lead that of the El Nino index at the best positive lag correlation between the two, and theformer 13 months lag behind the latter at their best negative lag correlation. Therefore the equatorialheat content can be used as a better predictor than the El Nino index for a warm or cold event. In addi-tion, it is also found that less heat anomaly in the equator corresponds to the stronger warm events inthe period (1980～1993) and much more heat was accumulated in the 4 years including 1992/1  相似文献   

双峰谱型海浪的波高和周期分布     

陈雪英 《海洋科学进展》1996,(2)

本文利用收集到的实测双峰谱型海浪过程资料，把这些资料以波高和周期的相关系数为参数分成５组，讨论每组双峰谱型下被高、周期的统计分布．并探讨了相关系数对波高分布和周期分布的影响。  相似文献   

西太平洋暖池区海—气通量计算分析   总被引：4，自引：0，他引：4  

徐天真  陈伯海 《中国海洋大学学报(自然科学版)》1993,(Z2)

用J.Launiaimen和T.Vihma提出的近地面层湍流通量计算方法,对我国在1992年11月至1993年2月TOGA—COARE—IOP实验中所获资料计算处理。得出所在站位的海一气间显效、潜热及动量通量。指出西大平洋暖池海区游热通量与显效通量之比为10.14:1;风速大于8m/s后各通量随风速的变化率明显增加;动量与热量的块体通量系数Cd和Ce,h随风速变化有相似的规律;Monin—Obukhov大气稳定度参数Z/L与△T/U_(10)之间有较好的统计关系。  相似文献   

对应分析方法在粤西水东湾动力沉积环境分析的应用     

陈子燊 《热带海洋学报》1991,(1)

本文以粤西水东湾表层沉积物样品的粒度分布为基本数据,使用对应分析方法并结合地形与波流特征,分析了海湾动力沉积环境。分析结果表明,海湾现代沉积环境可划分为4个动力沉积区。  相似文献   

鲁南近浅海沉积物样品的因子分析及其地质意义     

陈敬冉  林振宏 《中国海洋大学学报(自然科学版)》1988,(3)

本文提出河流携带入海的沉积物或沿岸剥蚀物中矿物的分布及再组合是受多种因素控制的。为了确定控制鲁南两城河至白马河沉积物样品中矿物分布及再组合的主要因素,作者对该区109个沉积物样品的重矿物资料进行了Q型和R型因子分析。研究指出,在诸多的河流中,白马河和两城河是控制该区沉积物分布的最上要的河流;矿物的化学稳定性是决定该区矿物再组合的主要因素。  相似文献   

High Precision Geoid Models HKGEOID-2000 for Hong Kong and SZGEOID-2000 for Shenzhen, China     

Zhicai Luo  Jinsheng Ning  Yongqi Chen  Zhanji Yang 《Marine Geodesy》2005,28(2):191-200

High precision geoid models HKGEOID-2000 for Hong Kong and SZGEOID-2000 for Shenzhen, China, have been developed with a hybrid approach of so-called sequential processing, using high precision GPS/leveling data, land and sea gravity anomalies, and digital terrain models. These two local geoid models have the same 1-km resolution. The estimated accuracy (external accuracy) is better than 1.7 cm for HKGEOID-2000 and 1.4 cm for SZGEOID-2000. Some common areas are covered by HKGEOID-2000 and SZGEOID-2000. So these two geoid models, along with high quality GPS/leveling data collected on the overlapping areas, can be used to detect the systematic bias between HKGEOID-2000 and SZGEOID-2000, as well as the difference between Hong Kong Principal Datum and 1956 yellow sea height datum of China, yielding RMS errors of 1.011 m and 1,003 m, respectively. Moreover, HKGEOID-2000, along with GPS ellipsoidal heights, is employed to determine the errors of the “orthometric heights” from purely trigonometric heighting, yielding an RMS error of 0.102 m. The combination of SZGEOID-2000 and GPS ellipsoidal heights has been used to replace the traditional spirit leveling and mapping, called GPS mapping.  相似文献   

Diagnosis of iridovirus in large yellow croaker by PCR   总被引：1，自引：0，他引：1  

Chen Xinhu  Wang Xiaowen  Wu Wenzhong 《海洋学报(英文版)》2003,22(4):635-642

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.  相似文献   

近海海面上水汽向上输送的季节性差异     

陈绍鑫 《海洋湖沼通报》1994,(2):202-207

本文分析了雷达观测结果，发现近海海面上的水汽向上输送随季节而变化，由于季节的变化和不同的天气情况，海表面大气现象在雷达中有不同的显示，根据这些显示得到了不同的信息，由此而得出春秋两季海表面水汽向上输送量的不同。  相似文献   

On the side wall effects upon bodies of arbitrary geometry in wave tanks     

Xiaobo Chen 《Applied Ocean Research》1994,16(6):337-345

A numerical model based on using a tank Green function, has been developed to compute the side wall effects on first- and second-order loadings upon bodies of arbitrary geometry in wave tanks. This tank Green function (TGF) is composed of a finite series of open-sea Green functions and an asymptotic part represented by two single integrals whose kernels decrease exponentially with the integral variable. This consistent expression of the TGF permits one to highlight the side wall effects and to give some criteria for the choice of tank width and the measurement duration to limit the reflection of diffraction and radiation waves.

The efficiency of the developed model is shown in the application to hemispheres and a box-shaped barge placed in the center of the wave tanks. The numerical results explain well the irregularities in the experimental measurements and show that the side walls have important effects on the first-order quantities. These effects are much more pronounced on the second-order drift loads.  相似文献