Oxidative damage to DNA: an immunohistochemical approach for detection of 7,8-dihydro-8-oxodeoxyguanosine in marine organisms     

Machella N  Regoli F  Cambria A  Santella RM 《Marine environmental research》2004,58(2-5):725-729

The modified nucleoside 7,8-dihydro-8-oxodeoxyguanosine (8-oxo-dG) is an index of oxidative DNA damage. An immunohistochemical approach based on the use of monoclonal antibody 1F7 against 8-oxo-dG was investigated in marine organisms with immunoperoxidase and immunofluorescent detection. Relative staining intensity as a measure of the 8-oxo-dG level was microscopically assessed. After laboratory exposures to benzo[a]pyrene (B[a]P), higher levels of oxidative DNA damage were clearly detected in all treated animals compared to controls. While this method eliminates DNA extraction reducing the processing of biological samples, absolute values are not provided. Further, the method requires only small amounts of tissue and potentially discriminates susceptibility to oxidative damage in different cell types. These results suggest that the assay should have practical applications in marine ecotoxicology.  相似文献   

Antioxidant efficiency and detoxification enzymes in spotted dogfish Scyliorhinus canicula     

Gorbi S  Pellegrini D  Tedesco S  Regoli F 《Marine environmental research》2004,58(2-5):293-297

Although elasmobranchs are widely distributed species, commonly found on sandy, gravely or muddy bottoms, several biological aspects of their metabolism still remain poorly investigated. In this work the efficiency of antioxidant system and detoxification enzymes were investigated in the coastal spotted dogfish Scyliorhinus canicula and in the red mullet Mullus barbatus for comparison with a teleost species. Organisms were sampled during a bottom trawl survey and analyzed for the biotransformation activity (EROD), levels of metallothioneins, catalase, glutathione S-transferases and total oxyradical scavenging capacity (TOSC) toward peroxyl radicals and hydroxyl radicals. EROD activity in the elasmobranchs was more than one order of magnitude lower than in the red mullets, while similar levels of metallothioneins were measured in these species. S. canicula showed significantly lower antioxidant enzymes and a more reduced efficiency in neutralizing *OH; on the other hand the scavenging capability toward ROO* was comparable in S. canicula and M. barbatus.  相似文献   

Disturbance and Community Pattern of Polychaetes Inhabiting Valle Magnavacca (Valli di Comacchio,Northern Adriatic Sea,Italy)     

Michele Mistri  Elisa A. Fano  Francesco Ghion  Remigio Rossi 《Marine Ecology》2002,23(1):31-49

Abstract. Polychaete community structure in Valle Magnavacca, the largest basin of the Valli di Comacchio (Northern Adriatic Sea) lagoonal ecosystem, was analyzed in a three‐year study. Three shallow‐water stations were sampled at about 3‐month intervals. Univariate and multivariate analyses were utilized to investigate community structure changes in relation to natural and man‐induced disturbance. The polychaete community at station 3, in the central area of the lagoon, was the most disturbed, while that of station 2 was the most well structured. We hypothesize that polychaete community structure results from regular seasonal disturbances which lead to almost predictable fluctuations in species abundance. Superimposed on these regular cycles are acute disturbance events that further reduce species abundance or exclude species from the habitat.  相似文献   

Wave-height distributions and nonlinear effects   总被引：2，自引：0，他引：2  

M. Aziz Tayfun  Francesco Fedele 《Ocean Engineering》2007,34(11-12):1631-1649

Theoretical distributions proposed for describing the crest-to-trough heights of linear waves are reviewed briefly. To explore the effects of nonlinearities, these are generalized to second-order waves, utilizing quasi-deterministic results on the expected shape of large waves. The efficacy of Gram–Charlier models in describing the effects of third-order nonlinearities on the distributions of wave heights, crests and troughs are examined in detail. All models and a fifth-order Stokes–Rayleigh type model recently proposed are compared with linear and nonlinear waves simulated from the JONSWAP spectrum representative of long-crested extreme seas, and also with oceanic data gathered in the North Sea. Uncertainties arising from the variability of probability estimates derived from sample populations of limited size are considered. Ultimately, the comparisons show that nonlinearities do not have any discernable effect on the crest-to-trough heights of oceanic waves. Most of the linear models considered yield similar and reasonable predictions of the observed data trends. Gram–Charlier type distributions seem neither effective nor particularly useful in describing the statistics of large wave heights or crests under oceanic conditions. However, they do surprisingly well in predicting unusually large wave heights and crests observed in some 2D wave-flume experiments and 3D numerical simulations of long-crested narrow-band random waves.  相似文献   

Magnesiochloritoid: Compressibility and high pressure structure refinement     

Paola Comodi  Marcello Mellini  Pier Francesco Zanazzi 《Physics and Chemistry of Minerals》1992,18(8):483-490

The response of magnesiochloritoid to pressure has been studied by single crystal X-ray diffraction in a diamond anvil cell, using crystals with composition Mg_1.3Fe_0.7Al₄Si₂O₁₀(OH)₄. The unit cell parameters decrease from a = 9.434 (3), b = 5.452 (2), c = 18.136 (5) Å, β = 101.42° (2) (1 bar pressure) to a = 9.370 (7), b = 5.419 (5), c = 17.88 (1) Å, β = 101.5° (1) (42 kbar pressure), following a slightly anisotropic compression pattern (linear compressibilities parallel to unit cell edges: β _a = 1.85, β _b = 1.74, β_c = 3.05 × 10^?4 kbar^?1) with a bulk modulus of 1480 kbar. Perpendicular to c, the most compressible direction, the crystal structure (space group C2/c) consists of two kinds of alternating octahedral layers connected via isolated SiO₄ tetrahedra. With increasing pressure the slightly wavy layer [Mg_1.3Fe_0.7AlO₂(OH)₄] tends to flatten. Furthermore, the octahedra in this layer, with all cations underbonded, are more compressible than the octahedra in the (A1₃O₈) layer with slightly overbonded aluminum. Comparison between high-pressure and high-temperature data yields the following equations: $$\begin{gathered} a_{P,T} = 9.434{\text{ }}{\AA} - 174 \cdot 10^{ - 5} {\text{ }}{\AA}{\text{kb}}^{{\text{ - 1}}} \cdot P \hfill \\ {\text{ }} + 9 \cdot 10^{ - 5} {\text{ }}{\AA}^\circ C^{ - 1} \cdot (T - 25^\circ C) \hfill \\ b_{P,T} = 5.452{\text{ }}{\AA} - 95 \cdot 10^{ - 5} {\text{ }}{\AA}{\text{kb}}^{{\text{ - 1}}} \cdot P \hfill \\ {\text{ }} + 5 \cdot 65 \cdot 10^{ - 5} {\text{ }}{\AA}^\circ C^{ - 1} \cdot (T - 25^\circ C) \hfill \\ c_{P,T} = 18.136{\text{ }}{\AA} - 549 \cdot 10^{ - 5} {\text{ }}{\AA}{\text{kb}}^{{\text{ - 1}}} \cdot P \hfill \\ {\text{ }} + 16 \cdot 2^{ - 5} {\text{ }}{\AA}^\circ C^{ - 1} \cdot (T - 25^\circ C) \hfill \\ \end{gathered} $$ with P in kbar and T in °C. These equations indicate that the unit cell and bond geometry of magnesiochloritoid at formation conditions do not differ greatly from those at the outcrop conditions, e.g. the calculated unitcell volume is 917.3 ų at P = 16 kbar and T=500 °C, whereas the observed volume at room conditions is 914.4 ų. In addition, they show that the specific gravity increases from formation at depth to outcrop at surface conditions.  相似文献   

Long-term growth and metropolitan spatial structures: an analysis of factors influencing urban patch size under different economic cycles     

Luca Salvati  Agostino Ferrara  Francesco Chelli 《Geografisk tidskrift / udgivet af Bestyrelsen for Det Kongelige danske geografiske selskab》2018,118(1):56-71

Urban forms are increasingly reflective of socio-economic change in metropolitan regions. The present study illustrates an original approach to identify latent mechanisms of urban growth through the analysis of metropolitan spatial structures and their proximate drivers of change. Urban transformations are evaluated here using built-up patches as the elementary analysis unit and background socio-economic attributes at four stages of the “city life cycle” (urbanization, suburbanization, dis-urbanization, re-urbanization). Regression models based on 13 indicators assessing urban patch area and shape, nearest neighbour urban patch area and shape, elevation, distance from 5 urban centres, 2 road infrastructures and sea coastline, were run with the aim to investigate direction and intensity of metropolitan growth in post-war Athens (1948–2012), a southern European city shifting from a compact mono-centric form towards discontinuous urban structures. Mono-centric configurations were characterized by linear dependence of urban patch size from the distance to central cities. Shifts toward scattered urban forms were associated with changes in the multivariate relationship between urban patch size and territorial drivers, pointing out the increased complexity of dispersed metropolitan structures. Our approach integrates economic and ecological visions of urban landscapes and contributes to understanding long-term mechanisms of metropolitan growth under dynamic spatial equilibriums. Investigating the multiple relationships between form and functions at the base of socio-economic transformations are relevant issues when identifying and profiling urbanization cycles.  相似文献   

3D displacement maps of the 2009 L’Aquila earthquake (Italy) by applying the SISTEM method to GPS and DInSAR data     

Francesco Guglielmino  Marco Anzidei  Pierre Briole  Panagiotis Elias  Giuseppe Puglisi 《地学学报》2013,25(1):79-85

  相似文献   

A thermo‐mechanical model for the catastrophic collapse of large landslides     

Francesco Cecinato  Antonis Zervos  Emmanuil Veveakis 《国际地质力学数值与分析法杂志》2011,35(14):1507-1535

In this work, a new thermo‐mechanical model is developed, applicable to large‐scale, deep‐seated landslides consisting of a coherent mass sliding on a thin clayey layer. The considered time window is that of catastrophic acceleration, starting at incipient failure and ending when the acquired displacement and velocity are such that the sliding material begins to break up into pieces. The model accounts for temperature rise in the slip zone due to the heat produced by friction, leading to water expansion, thermoplastic collapse of the soil skeleton, and subsequent increase of pore water pressure. The model incorporates the processes of heat production and diffusion, pore pressure generation and diffusion, and an advanced constitutive law for the thermo‐mechanical behavior of soil. An analysis of the Vajont landslide is presented as an example. A sensitivity analysis shows that friction softening is the mechanism most affecting the timescale of the final collapse of a slide, but also that the mechanism of thermal pressurization alone can cause a comparably catastrophic dynamic evolution. It is also shown that, all other factors being equal, thermo‐mechanical collapse will cause thicker slides to accelerate faster than shallow ones. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献   

Detection of Miocene saucer‐shaped sills (offshore Senegal) via integrated interpretation of seismic,magnetic and gravity data     

Sergio Rocchi  Alfredo Mazzotti  Michele Marroni  Luca Pandolfi  Paolo Costantini  Bertozzi Giuseppe  Davide di Biase  Francesco Federici  Papa Goumbo L 《地学学报》2007,19(4):232-239

The Atlantic margin offshore Senegal has been explored by seismic reflection and GRAV‐MAG surveys. High‐amplitude, laterally transgressive seismic reflectors are found to coincide with gravimetric and magnetic highs. Once seismic data are integrated with potential fields modelling, these reflectors can be safely interpreted as saucer‐shaped igneous sills, up to some hundreds of metres thick, some km wide. The occurrence of hydrothermal vent complexes and forced folds in the stratigraphic sequence above the sills constrain the intrusion age to the Miocene. Field observations and in‐situ magnetic susceptibility measurements of Oligocene–Miocene and Quaternary igneous rocks emplaced in coastal Senegal support this interpretation.  相似文献   

Job losses and political acceptability of climate policies: why the ‘job-killing’ argument is so persistent and how to overturn it     

Francesco Vona 《Climate Policy》2019,19(4):524-532

Political acceptability is an essential issue in choosing appropriate climate policies. Sociologists and behavioural scientists recognize the importance of selecting environmental policies that have broad political support, while economists tend to compare different instruments first on the basis of their efficiency, and then by assessing their distributional impacts and thus their political acceptability. This paper examines case-study and empirical evidence that the job losses ascribed (correctly or incorrectly) to climate policies have substantial impacts on the willingness of affected workers to support these policies. In aggregate, the costs of these losses are significantly smaller than the benefits, both in terms of health and, probably, of labour market outcomes, but the losses are concentrated in specific areas, sectors and social groups that have been hit hard by the great recession and international competition. Localized contextual effects, such as peer group pressure, and politico-economic factors, such as weakened unions and tightened government budgets, amplify the strength and the persistence of the ‘job-killing’ argument. Compensating for the effects of climate policies on ‘left-behind’ workers appears to be the key priority to increase the political acceptability of such policies, but the design of compensatory policies poses serious challenges.

Key policy insights

• Public perception of, and support for, climate policies is substantially reduced in the presence of large negative shocks, especially job losses.

• Climate policies can be perceived as negative for employment, especially in areas where polluting industries represent a large share of employment and in occupations and sectors already damaged by globalization and automation.

• Policymakers should distinguish between small and large distributional effects of climate policies, and find the appropriate combination of revenue recycling schemes, industrial and retraining policies as well as compensation packages to increase the support for such policies.

  相似文献