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31.
LUO Yiwen AI Qinghui MAI Kangsen ZHANG Wenbing XU Wei ZHANG Yanjiao LIUFU Zhiguo 《中国海洋大学学报(英文版)》2013,12(3):418-426
A growth experiment was conducted on cobia(Rachycentron canadum,initial weight 108.2 g ± 3.0 g) to investigate the effects of dietary corn gluten meal(CGM) levels on the fish growth,whole body composition and protein metabolism in relation to specific gene expression.Five isonitrogenous(crude protein 45%) and isoenergetic(gross energy 20 kJ g 1) practical diets were formulated by replacing 0%(the control),17.5%,35.0%,52.5%,and 70.0% of fish meal(FM) protein with CGM protein.No significant differences were observed in the survival,feed intake(FI),specific growth rate(SGR),feed efficiency(FE) and protein productive value(PPV) among fish fed diets with 0%,17.5%,35.0%,and 52.5% of CGM protein.However,these indices were significantly lower in fish fed the diet with 70.0% of CGM protein than those in fish fed the control diet(P < 0.05).The whole-body crude protein and lipid contents were significantly lower while the whole-body moisture content was significantly higher in fish fed the diet with 70.0% of CGM protein compared with the control group(P < 0.05).When 70.0% of FM protein was replaced by CGM,plasma total protein and cholesterol contents were significantly lower than those in the control group(P < 0.05).Fish fed the diet with 70.0% of CGM protein had significantly lower hepatic insulin-like growth factor I(IGF-I) expression levels than those in the control group(P < 0.05).However,no significant differences were observed in hepatic target of rapamycin(TOR),dorsal muscle IGF-I and TOR expression levels among dietary treatments.Results of the present study indicated that 52.5% of FM protein could be replaced by CGM in the diets without significant influences on the growth,feed utilization and protein metabolism of juvenile cobia.The present results might be useful for developing cost effective and sustainable cobia dietary formulations. 相似文献
32.
在动力机器基础测试中,拉伸回弹法是一种简易实用的瞬态激振方法;它比常用的球击法具有激振能量大,信号接收效果佳等优点。但运用该法如何求地基刚度的计算却不多见,故本文简要地列出我们推导的算式,以供参考与探讨。 相似文献
33.
The dietary protein requirement of juvenile turbot(initial average weight, 38.2 g ± 0.1 g) reared indoor in aerated aquaria was determined in this study. Five energy equal experimental diets were formulated with fish meal as protein source, which contained different concentrations of protein(47.2%, 51.0%, 54.6%, 59.3% and 63.6% of dry diet). Three groups of fish with 18 individuals in each, were cultured in 300-L tanks and fed twice a day for 8 weeks. During culture, temperature was controlled between 15.0 and 18.0℃, salinity was controlled between 28.5 and 32.0, acidity was controlled between p H7.8 and p H8.5, and ammonia nitrogen was maintained below 0.03 mg L-1 and dissolved oxygen was maintained about 7 mg L-1. Results showed that the growth of fish was significantly affected by dietary protein content(P 0.05). Specific growth rate(SGR) of turbot increased when dietary protein content varied between 47.2% and 51.0%(P 0.05), and then kept stable when dietary protein content was higher than 51.0%. Fish which were fed the diet containing 63.6% protein showed the highest SGR while those fed the diet containing 59.3% protein showed the highest feed efficiency rate. No significant difference of feed intake and protein efficiency ratio was found among experimental diets(P 0.05). Broken-line regression analysis of SGR showed that the optimal dietary protein requirement of turbot was about 57.0%. 相似文献
34.
ZHANG Kaikai MAI Kangsen XU Wei ZHOU Huihui LIUFU Zhiguo ZHANG Yanjiao PENG Mo 《中国海洋大学学报(英文版)》2015,14(3)
This study was conducted to investigate the effect of dietary proline(Pro), and Pro and hydroxyproline(Hyp) in combination on the growth performance, total Hyp and collagen concentrations of tissues, and prolyl 4-hydroxylase α(I)(P4H α(I)) gene expression in juvenile turbot feeding high plant protein diets. A diet containing 50% crude protein and 12% crude lipid was formulated as the basal and control, on which other two protein and lipid contents identical experimental diets were formulated by supplementing the basal with either 0.75% Pro(Pro-0.75) or 0.75% Pro and 0.75% Hyp(Pro+Hyp). Four groups of fish in indoor seawater recirculating systems, 35 individuals each, were fed twice a day to apparent satiation for 10 weeks. The results showed that dietary Pro and Hyp supplementation had no significant effect on growth performance and feed utilization of juvenile turbot(P 0.05). Total Hyp and collagen concentrations in muscle were significantly increased when dietary Pro and Hyp increased(P 0.05), and fish fed diet Pro+Hyp showed significantly higher free Hyp content in plasma than those fed other diets(P 0.05). The expression of P4 H α(I) gene in liver and muscle was significantly up regulated in fish fed diet Pro-0.75 in comparison with control(P 0.05); however the gene was significantly down regulated in fish fed diet Pro+Hyp in muscle in comparison with fish fed diet Pro-0.75(P 0.05). It can be concluded that supplement of crystal L-Pro and L-Hyp to high plant protein diets did not show positive effects on growth performance of juvenile turbot, but enhanced total collagen concentrations in muscle. 相似文献
35.
LUO Zhi LIU Yongjian MAI Kangsen and TIAN Lixia Institute of Aquatic Economic Animals School of Life Sciences Sun Yat-sen University Guangzhou P.R.China Fisheries College Ocean University of China Qingdao P.R.China 《中国海洋大学学报(英文版)》2005,4(2)
The paper reviews the recent advances in studying grouper nutrition requirement for the development of cost-effective and environmentally friendly artificial diets. It consists of seven parts: protein and amino acid, lipid and essential fatty acid, carbohydrate, vitamin, mineral, alternative protein source, broodstock and larval nutrition. The review provides some basic information for further investigation of nutrient requirements of groupers. 相似文献
36.
迟钝爱德华氏菌(Edwardsiella tarda)研究概况 总被引：14，自引：0，他引：14
迟钝爱德华氏菌(Edwardsiella tarda)是水产养殖中危害极大的病原菌,本文从发病情况、致病性研究及防治等诸方面将国内外对该菌的研究情况进行了系统的介绍. 相似文献
37.
不同培养基对筒柱藻Cylindrotheca fusiformis生长及脂肪酸组成的影响 总被引：9，自引：0，他引：9
在温度为22±1℃,盐度为28的条件下,同不同培餐基对青岛海洋大学微藻种质库保存的简柱藻Cylindrotheca fusiformis造行培养,测定了细胞的生长情况及脂肪酸组成.实验结果表明,用8F培养基((F/2培养基的16倍)培养5天后,达到的细胞密度最大(376.56×104细胞/mL).用2F培养基培养,EPA含量最高(占总脂肪酸的18.1%).细胞生长较好、EPA含量最高、成本较低的培养基是2F培养基. 相似文献
38.
Weifang Wang Kangsen Mai Wenbing Zhang Wei Xu Qinghui Ai Chunfeng Yao Huitao Li 《中国海洋大学学报(英文版)》2009,8(3):254-258
A study was conducted to evaluate the effects of dietary carbohydrates on triglyceride, cholesterol and fatty acid concentrations
in abalone, Haliotis discus hannai Ino. Six semi-purified diets with different carbohydrates (dextrin, heat-treated wheat starch, wheat starch, corn starch,
tapioca starch and potato starch, respectively), all containing a carbohydrate level of 33.5%, were fed to abalone (initial
shell length: 29.98 mm ± 0.09 mm; initial weight: 3.42 g ± 0.02 g) for 24 weeks in a recirculation system. The results indicate
that serum triglyceride concentrations were significantly (P < 0.05) higher in the abalone fed with dextrin, heat-treated wheat starch and wheat starch than those fed with corn starch,
and serum cholesterol concentrations were significantly (P < 0.05) higher in the abalone fed with dextrin, heat-treated wheat starch than those fed with corn starch. Fatty acid C20:4n-6
in the foot muscles were significantly (P < 0.05) lower in the abalone fed with dextrin than those fed with wheat starch, corn starch, tapioca starch and potato starch.
Fatty acid C20:4n-6 in hepatopancreas was significantly (P < 0.05) lower in abalone fed with heat-treated wheat starch than those fed with corn starch, tapioca starch and potato starch.
Fatty acid C22:6n-3 in the foot muscles were significantly (P < 0.05) lower in the abalone fed with dextrin and heat-treated wheat starch than those fed with wheat starch and potato starch. 相似文献
39.
WANG Weifang MAI Kangsen ZHANG Wenbing XU Wei AI Qinghui YAO Chunfeng LI Huitao 《中国海洋大学学报(英文版)》2009,8(3)
A study was conducted to evaluate the effects of dietary carbohydrates on triglyceride, cholesterol and fatty acid concentrations in abalone, Haliotis discus hannai Ino. Six semi-purified diets with different carbohydrates (dextrin, heat-treated wheat starch, wheat starch, corn starch, tapioca starch and potato starch, respectively), all containing a carbohydrate level of 33.5%, were fed to abalone (initial shell length: 29.98 mm±0.09 mm; initial weight. 3.42 g±0.02 g) for 24 weeks in a recirculation system. The results indicate that serum triglyceride concentrations were significantly (P<0.05) higher in the abalone fed with dextrin, heat-treated wheat starch and wheat starch than those fed with corn starch, and serum cholesterol concentrations were significantly (P<0.05)higher in the abalone fed with dextrin, heat-treated wheat starch than those fed with corn starch. Fatty acid C20:4n-6 in the foot muscles were significantly (P<0.05) lower in the abalone fed with dextrin than those fed with wheat starch, corn starch, tapioca starch and potato starch. Fatty acid C20:4n-6 in hepatopancreas was significantly (P<0.05) lower in abalone fed with heat-treated wheat starch than those fed with corn starch, tapioca starch and potato starch. Fatty acid C22:6n-3 in the foot muscles were significantly (P< 0.05) lower in the abalone fed with dextrin and heat-treated wheat starch than those fed with wheat starch and potato starch. 相似文献
40.
Studies were conducted to investigate the non-specific immune responses and disease-resistance of juvenile turbot Scophthalmus maximus,cultured at four different salinities(8,20,32 and 40) . Three concentrations(3.75 × 10 7,3.75 × 10 8 and 3.75 × 10 9 CFU/ml) of Vibrio anguillarum suspension were employed at each salinity to determine the 4-day LD 50 . The serum lysozyme activity,the alternative complement pathway activity(ACH50) and the phagocytosis percentage of head kidney in turbot were tested at 24,48 and 72 h post-challenge of V. anguillarum(1.1 × 10 8 CFU/ml,0.1 ml) ,respectively,to evaluate the non-specific immune responses at the selected rearing salinities. Fish reared at salinity 20 had the lowest mortality,namely,the highest 4-day LD 50 value(8.88 ± 0.17) . Besides,the lysozyme activity,ACH50 and the phagocytosis of turbot were the highest at the salinity 20,but with the lowest at the salinity 40 treatment regardless of sampling time. In addition,the non-specific immune activities kept increasing within 72 h post-challenge of V. anguillarum,except that the lysozyme activity increased from 24 to 48 h,and then decreased from 48 to 72 h at 40 significantly. These results together indicate that rearing in intermediate salinity(20) was able to enhance the immunity and disease-resistance of turbot. 相似文献