Development of three multiplex PCR primer sets for ark shell (<Emphasis Type="Italic">Scapharca broughtonii</Emphasis>) and their validation in parentage assignment     

Ning Li  Qi Li  Lingfeng Kong  Hong Yu 《中国海洋大学学报(英文版)》2016,15(2):311-317

Scapharca broughtonii is a commercially important and over-exploited species. In order to investigate its genetic diversity and population structure, 43 novel polymorphic microsatellites were isolated and characterized. The number of alleles per locus ranged from 3 to 22 with an average of 6.93, and the observed and expected heterozygosities varied between 0.233 and 1.000, and 0.250 and 0.953, with an average of 0.614 and 0.707, respectively. Three highly informative multiplex PCRs were developed from nine of those microsatellites for S. broughtonii. We evaluated and validated these multiplex PCRs in 8 full-sib families. The average polymorphism information content (PIC) was 0.539. The frequency of null alleles was estimated as 3.13% of all the alleles segregation based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 100% of all offspring were unambiguously allocated to a pair of parents based on 3 multiplex sets. Those 43 microsatellite loci with high variability will be helpful for the analysis of population genetics and conservation of wild stock of S. broughtonii. The 3 sets of multiplex PCRs could be an important tool of pedigree reconstruction, population genetic analysis and brood stock management.  相似文献   

An Investigation of Digestion Methods for Trace Elements in Bauxite and Their Determination in Ten Bauxite Reference Materials Using Inductively Coupled Plasma‐Mass Spectrometry          下载免费PDF全文

Wen Zhang  Liang Qi  Zhaochu Hu  Cunjiang Zheng  Yongsheng Liu  Haihong Chen  Shan Gao  Shenghong Hu 《Geostandards and Geoanalytical Research》2016,40(2):195-216

Trace elements from samples of bauxite deposits can provide useful information relevant to the exploration of the ore‐forming process. Sample digestion is a fundamental and critical stage in the process of geochemical analysis, which enables the acquisition of accurate trace element data by ICP‐MS. However, the conventional bomb digestion method with HF/HNO₃ results in a significant loss of rare earth elements (REEs) due to the formation of insoluble AlF₃ precipitates during the digestion of bauxite samples. In this study, the digestion capability of the following methods was investigated: (a) ‘Mg‐addition’ bomb digestion, (b) NH₄HF₂ open vessel digestion and (c) NH₄F open vessel digestion. ‘Mg‐addition’ bomb digestion can effectively suppress the formation of AlF₃ and simultaneously ensure the complete decomposition of resistant minerals in bauxite samples. The addition of MgO to the bauxite samples resulted in (Mg + Ca)/Al ratios ≥ 1. However, adding a large amount of MgO leads to significant blank contamination for some transition elements (V, Cr, Ni and Zn). The NH₄HF₂ or NH₄F open vessel digestion methods can also completely digest resistant minerals in bauxite samples in a short period of time (5 hr). Unlike conventional bomb digestion with HF/HNO₃, the white precipitates and the semi‐transparent gels present in the NH₄HF₂ and NH₄F digestion methods could be efficiently dissolved by evaporation with HClO₄. Based on these three optimised digestion methods, thirty‐seven trace elements including REEs in ten bauxite reference materials (RMs) were determined by ICP‐MS. The data obtained showed excellent inter‐method reproducibility (agreement within 5% for REEs). The relative standard deviation (% RSD) for most elements was < 6%. The concentrations of trace elements in the ten bauxite RMs showed agreement with the limited certified (Li, V, Cr, Cu, Zn, Ga, Sr, Zr and Pb) and information values (Co, Ba, Ce and Hf) available. New trace element data for the ten RMs are provided, some of which for the first time.  相似文献   

Coupled discrete element–finite difference method for analyzing subsidence control in fully mechanized solid backfilling mining     

Xiaojun Zhu  Guangli Guo  Qi Fang 《Environmental Earth Sciences》2016,75(8):683

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Rotation Center and Horizontal Bearing Capacity of the Bucket Foundation in Soft Ground     

Xiaowei Tang  Xiwen Zhang  Qi Shao  Zhenqing Li 《Marine Georesources & Geotechnology》2016,34(6):594-601

In the last 20 years, the bucket foundation has been developed as a new type of offshore platform structure. Because of its short period of application to engineering practices, the theoretical decision on the rotation center and horizontal bearing capacity of the bucket foundation has yet to be agreed. A limit analysis method is used to determine the updated rotation center position and horizontal bearing capacity to evaluate the failure mechanisms of the bucket foundations. The results are compared with numerical simulation and experiments, and also with other theoretical methods. The proposed method can satisfactorily consider the engineering conditions and the result is accurate in determining the rotation center and horizontal bearing capacity.  相似文献   

Influencing factors of seed long-distance dispersal on a fragmented forest landscape on Changbai Mountains,China     

Fuqiang Zhao  Lin Qi  Lei Fang  Jian Yang 《中国地理科学(英文版)》2016,26(1):68-77

Seed long-distance dispersal(LDD) events are typically rare, but are important in the population processes that determine large-scale forest changes and the persistence of species in fragmented landscapes. However, previous studies focused on species dispersed via animal-mediated LDD, and ignored those dispersed by wind. The aim of this study was to assess the effects of canopy openness, edge, seed source, and patch tree density on the LDD of seeds by wind in forest. We collected birch seeds, a typical wind-dispersed species, throughout a larch plantation. We then assessed the relationship between birch LDD and each factor that may influence LDD of seeds by wind including distance to edge, canopy openness size, distance to mature forest, and the tree density of the larch plantation. We used univariate linear regression analysis to assess the influence of those factors on birch LDD, and partial correlations to calculate the contribution of each factor to LDD. The results showed that both canopy openness and edge had significant influences on birch LDD. Specifically, a negative relationship was observed between distance to edge and birch LDD, whereas there was a positive correlation between canopy openness size and LDD. In contrast, the distance to the mature forest was not correlated with birch LDD. Our results suggest that patch tree density could potently affect the probability of LDD by wind vectors, which provides novel and revealing insights regarding the effect of fragmentation on wind dynamics. The data also provide compelling evidence for the previously undocumented effect of habitat fragmentation on wind-dispersed organisms. As such, these observations will facilitate reasonable conservation planning, which requires a detailed understanding of the mechanisms by which patch properties hamper the delivery of seeds of wind-dispersed plants to fragmented areas.  相似文献   

Identification and determination of the boundary between the Cathaysian and Yangtze blocks: a preliminary understanding of gravity and magnetic fields     

Jiayong Yan  Kun Zhang  Guangming Fu  Fan Luo  Hao Wang  Guang Qi 《《地质学报》英文版》2018,92(Z1):222-225

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考虑方位漂移的水平井井眼轨道设计   总被引：1，自引：1，他引：0       下载免费PDF全文

王世栋  张志刚  耿雪樵  于彦江  陈奇 《探矿工程》2018,45(7):14-18

目前,深层页岩气水平井在钻井过程中经常出现方位漂移的问题,方位漂移严重影响了水平井的井眼轨迹控制,进而影响水平井的准确中靶。在现场施工中,通常根据施工区以前的钻井经验,在进行定向造斜时,先估计出一个“方位超前角”,这种现场的做法有着很大的不确定性,并且可能出现较大误差。本文提出了考虑方位漂移的水平井井眼轨道优化设计的方法,解决了仅凭现场经验给出的“超前角”不准确的问题。考虑方位漂移的水平井井眼轨道优化设计按照井段划分,将地层因素与井身剖面结合起来求取井段的平均方位漂移率。根据该方法研制开发了考虑方位漂移的水平井井眼轨道优化设计软件,并通过举例进行了验证,证明了方法是可行的。  相似文献   

地质导向技术在L型煤层气水平井 T-P05井中的应用          下载免费PDF全文

郭宝林  李琪  张兴龙  聂涛  于建克  李盟国 《探矿工程》2018,45(1):9-13

通过分析山西L型煤层气水平井的技术难点,着重建立造斜着陆与水平段导向两大导向模型,形成了以标志层划分与对比、地层倾角推算、地质建模和实时轨迹控制四个方面为主的L型煤层气水平井地质导向技术流程,该技术在T-P05井的成功应用,不仅丰富了煤层气水平井地质导向技术的内容与方法,更进一步验证了该技术在现场具有较强的可操作性和良好的应用前景,值得大力推广。  相似文献   

贵州松桃高地特大型富锰矿床主要地质特征   总被引：1，自引：1，他引：0       下载免费PDF全文

袁良军  周琦  姚希财  谢小峰  杜远生  张遂  潘文  谢兴友  王萍  徐源 《贵州地质》2018,35(4):314-318

位于贵州铜仁松桃国家锰矿整装勘查区内的高地富锰矿床,(332)+(333)类碳酸锰富锰矿石资源量达7 166. 84万吨,锰平均品位25. 75%,矿体平均厚度4. 52 m,富锰矿体赋存于总资源量达1. 61亿吨的高地超大型锰矿床之中,由一个单一的富锰矿体构成,是我国新发现的首个特大型富锰矿床,富锰矿资源量相当于我国富锰矿资源量总和的2倍。成因类型属古天然气渗漏沉积型锰矿床,富锰矿产于古天然气渗漏沉积成矿系统中心相区。高地富锰矿床地处独特的"新月形"南华纪早期李家湾-高地-道坨(Ⅳ级)地堑盆地中心,菱锰矿体中均出现以块状构造夹被沥青充填的气泡状富菱锰矿石。地表燕山期北北东向的断裂构造十分发育,但主要为上陡下缓犁式正断层,未触及高地深部隐伏巨型锰矿体,以致富锰矿体保存亦十分完整,易于开发利用。  相似文献   

黔东及毗邻地区南华纪锰矿成矿期与成矿后构造专项填图方法研究          下载免费PDF全文

谢小峰  周琦  袁良军  张遂  杨炳南  谢兴友  刘健 《贵州地质》2018,35(4):376-382

黔东及毗邻地区南华纪锰矿成矿期构造主要为同沉积断层,该类型断层在成矿期十分发育,控制和形成了南华纪早期不同序次的次级裂谷盆地,同沉积断层的垂向发育形成了锰矿成矿通道,同沉积断层可作为古天然气渗漏沉积型锰矿床关键地质找矿标志,本文阐述了其主要判别特征。南华纪锰矿形成后,历经加里东运动、印支运动及燕山运动等构造运动,总体来说,该区构造格局主要是受到燕山运动影响形成。本文也讨论了研究区成矿后广泛发育且比较典型,对锰矿层保存与破坏有着较大影响的犁式正断层及其判别特征。  相似文献