凡纳滨对虾C-型凝集素LvLec2 对不同刺激的免疫应答     

罗展  张继泉  李富花  柳承璋  相建海 《海洋科学》2010,34(11):103-110

克隆获得了与中国明对虾(Fenneropenaeus chinensis)C-type lectin 3同源的C-型凝集素LvLec2基因序列,并通过Real-time PCR研究了其在脂多糖(lipopolysaccharide,简称LPS)、灭活溶壁微球菌(Micrococcus lysodeikticus)和白斑综合症病毒(white spot syndrome virus,简称WSSV)刺激后的转录表达变化。结果表明,LvLec2编码157个氨基酸,含有1个糖识别结构域(carbohydrate recognition domain,简称CRD),其CRD结构域中具有决定糖结合特异性的基序"EPS"(Glu118-Pro119-Ser120)序列。系统进化树分析表明LvLec2与已发表的凡纳滨对虾(Litopenaeus vannamei)C-型凝集素亲缘较远。LPS、灭活溶壁微球菌和WSSV刺激后,LvLec2基因在肝胰脏中的转录表达有明显的变化但表达模式不同。LvLec2可能作为模式识别受体参与了对虾对病原的识别或防御。  相似文献   

放牧对浑善达克沙地丘间低地植被群落及土壤的影响   总被引：1，自引：1，他引：0  

詹瑾  李玉霖  韩丹  杨红玲 《中国沙漠》2019,39(6):184-191

为探究放牧对丘间低地植被群落及土壤的影响,分析了浑善达克沙地丘间低地在重度和轻度放牧下植被的地上生物量、物种重要值、物种丰富度、Shannon-Wiener多样性指数、Pielou均匀度指数、Simpson生态优势度指数及0~10 cm土壤中黏粒、粉粒、砂粒、有机碳和全氮含量。结果表明：（1）植被地上生物量、土壤黏粒、粉粒、砂粒、土壤有机碳和全氮含量在不同放牧程度下存在极显著差异（P<0.01）,物种丰富度、Shannon-Wiener多样性指数和Pielou均匀度指数在不同放牧程度下差异不显著（P>0.05）,Simpson生态优势度指数在不同放牧程度下差异显著（P<0.05）。（2）放牧区植被以禾本科为主,禾本科植被地上生物量占整个植被群落的54.88%~57.76%;重度放牧区植被地上生物量为63.59 g·m^-2,比轻度放牧区低26.61%。（3）羊草（Leymus chinensis）和狗尾草（Setaria viridis）是放牧区的优势种,其重要值依次为14.16%（重度放牧区）和19.10%（轻度放牧区）、13.40%（重度放牧区）和15.42%（轻度放牧区）,重度放牧下雾滨藜（Bassia dasyphylla）、克氏针茅（Stipa krylovii）和猪毛蒿（Artemisia scoparia）的重要值较显著高于轻度放牧区,而羊草则低于轻度放牧区。（4）重度放牧加剧了土壤沙漠化,使砂粒含量增加,草地生产力下降,土壤有机碳和全氮含量降低。  相似文献   

Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency     

Aibin Zhan  Zhenmin Bao  Xiaoli Hu  Wei Lu  Jingjie Hu 《中国海洋大学学报(英文版)》2009,8(2):161-165

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.  相似文献   

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郭良迁  占伟  杨国华  薄万举 《大地测量与地球动力学》2010,30(4):36-42

???????GPS????????????????????λ?????????????????о??????????????????С????α??????????????????????仯???????????繤?????????????????????????????????????????????????￡??????????????????????????????????????仯??????????????λ????????????????????????????????е????????????С???????????????????С?????α?????й?????????????????????????????????????????й??????????С?????α????????????С????????????????????????????????????????????2006??2009??????????????????????????????????????????????????С???,?????????????????????????????????????????????????????仯???????????????????????????  相似文献   

A novel dynamic stretching solution to eliminate saturation effect in NDVI and its application in drought monitoring     

Fang Liu  Qiming Qin  Zhiming Zhan 《中国地理科学(英文版)》2012,22(6):683-694

The normalized difference vegetation index(NDVI) is one of the key input variables for developing drought indices.However,the NDVI quickly saturates in high vegetation surfaces,and thus,the generalization of a drought index over different ecosystems becomes a challenge.This paper presents a novel,dynamic stretching algorithm to overcome the saturation effect in NDVI.A scaling transformation function to eliminate saturation effects when the vegetation fraction(VF) is large is proposed.Dynamic range adjustment is conducted using three coefficients,namely,the normalization factor(a),the stretching range controlling factor(m),and the stretching size controlling factor(e).The results show that the stretched NDVI(S-NDVI) is more sensitive to vegetation fraction than NDVI when the VF is large,ranging from 0.75 to 1.00.Moreover,the saturation effect in NDVI is effectively removed by using the S-NDVI.Further analysis suggests that there is a good linear correlation between the S-NDVI and the leaf area index(LAI).At the same time,the proposed S-NDVI significantly reduces or even eliminates the saturation effect over high biomass.A comparative analysis is performed between drought indices derived from NDVI and S-NDVI,respectively.In the experiment,reflectance data from the moderate resolution imaging spectroradiometer(MODIS) products and in-situ observation data from the meteorological sites at a regional scale are used.In this study,the coefficient of determination(R2) of the stretched drought index(S-DI) is above 0.5,indicating the reliability of the proposed algorithm with surface soil moisture content.Thus,the S-DI is suggested to be used as a drought index in extended regions,thus regional heterogeneity should be taken into account when applying stretching method.  相似文献   

无拉伸动校正技术在近海工程地震勘探中的应用          下载免费PDF全文

孟庆生  樊玉清  郭秀军  贾永刚  马志杰  刘展 《地球物理学进展》2007,22(6):1889-1894

动校正是地震资料处理的关键内容之一,直接关系到地震资料处理结果的精度.在浅层和大偏移距情况下,常规动校正使波形发生拉伸畸变,波形拉长、频带向低频方向移动,进而影响叠加效果.通常地震资料处理中大都采用切除的方法克服动校正拉伸畸变的影响,这对目的层较深时是可取的.以工程地质调查为目的的海洋地震勘探旨在了解海底之下较浅地层深度范围内的地质信息,一般勘探区水深较浅,数据叠加道数较少,如果仍然采取切除处理,势必严重影响资料的分辨率和准确度.本文阐述了一种无拉伸动校正方法,能较好地解决动校正后的波形畸变问题,理论模型和实际资料的处理结果表明该方法在近海浅层工程地震勘探中是行之有效的,有助于提高速度分析的精度和地震资料的分辨率.  相似文献   

宝顶石刻区污染地下水对造像岩体的破坏作用     

张赞勋  汪东云  付林森  姚金石  谢本立 《山地学报》1994,(3)

污染地下水渗入大佛湾摩岩造像群，在石刻表面积聚可溶盐，加剧了石刻的侵蚀破坏，其中尤以泥质细粒长石砂岩风化剥落最为严重。研究渗水病害对石刻保护有重要意义。  相似文献   

磁性下界面的正则化反演方法   总被引：1，自引：0，他引：1  

刘展  潘作枢 《物探与化探》1995,19(6):439-448

根据磁异常反演磁性介质下界面,属于非线性问题,一般很难直接求解.本文采用将非线性问题线性化措施,建立起线性离散磁模型,然后应用线性规划的正则算法,设计了迭代求解方案.该方法不要求已知界面起伏的平均深度,只要求假定界面是连续的及磁性沿横向变化.算法便于对解施行物理约束,具有收敛快,精度高的特点.  相似文献   

关于部分变元的非常稳定性     

湛少锋 《武汉大学学报(信息科学版)》1990,(4)

本文利用极限方程的方法研究微分方程关于部分变元的多种非常稳定性,讨论了给定方程与其积限方程的非常稳定性的关系。  相似文献   

地磁干扰噪声的实验观测   总被引：1，自引：0，他引：1       下载免费PDF全文

詹志佳  高金田  郭启华  胡荣盛  张洪利  赵从利  沈文志 《地震工程学报》1990,12(4):49-54

为探查1988年3月前后在北京地磁台附近出现的较明显的地磁干扰噪声,我们在苏家坨—卧龙岗一带布设了8个测点,开展了专门的地磁干扰噪声实验观测。结果表明,该地磁干扰噪声主要是来自香山一带的电磁干扰,其次是北京市的地铁干扰。此地磁干扰噪声出现于每天06~h—23~h(北京时),其主要频谱成分在短周期(T=2—4 m)部分。另外本文的观测研究方法对于监测地磁台站(测点)的环境状况,改正受污染的地磁资料等方面具有实际应用价值。  相似文献