GNSS多系统组合精密定位研究进展          下载免费PDF全文

柴洪洲  潘宗鹏  崔岳 《海洋测绘》2016,(4):21-26

近年来,我国北斗卫星导航系统(BDS)以及欧洲伽利略卫星导航系统(GALILEO)正在快速建设与发展,同时,GPS和GLONASS卫星导航系统的现代化也在逐步推进,卫星导航定位领域正发生着深刻的变革。系统介绍了各卫星导航系统的建设和发展状况,探讨了多系统组合定位带来的机遇和面临的挑战,以及当前对这些问题的研究现状,最后对多系统组合定位的应用前景进行展望。  相似文献   

深海细菌太平洋火色杆菌中α-淀粉酶基因amy608的克隆表达及其酶学性质研究          下载免费PDF全文

商谢谢  蔡亚萍  金敏  曾润颖 《应用海洋学学报》2016,35(1):130-137

从来自深海沉积物的太平洋火色杆菌(Flammeovirga pacifica)的基因组中发现了一个全长为1 875 bp的α-淀粉酶基因amy608.该基因在数据库中找不到具有同源性的序列,而所编码的Amy608蛋白与已注册蛋白的氨基酸序列相似性最高仅为56%,但具有α-淀粉酶水解活性所必需的保守基序DXEXD.进化树分析表明其属于糖苷水解酶13(GH13)家族第二亚家族.构建了p ColdΙ-amy608表达载体,在大肠杆菌中进行重组Amy608蛋白的异源表达,并采用镍离子亲和层析柱对重组蛋白进行了纯化.酶学性质分析表明,重组酶Amy608的最适作用温度为40℃,在40℃保温4 d后,仍保留70%以上的酶活,显示出良好的中温热稳定性;最适p H值为7.0,p H值范围在6~9时仍保留60%以上的酶活力,表明该酶具有较宽的p H值作用范围.Ca2+、Na+、K+对α-淀粉酶Amy608有激活作用,尤其是Ca2+可使酶活显著提高40%.薄层色谱分析结果显示该酶水解可溶性淀粉的最终产物以葡萄糖为主,表明该酶是一个糖化型淀粉水解内切酶.这些结果表明Amy608为GH13家族第二亚家族中一个新型的α-淀粉酶.  相似文献   

深海细菌Pseudomonas sp. IOFA1中甲醛歧化酶FM11的纯化及性质研究          下载免费PDF全文

余翔  陈兴麟  金敏  曾润颖 《应用海洋学学报》2016,35(3):418-425

从印度洋深海沉积物中分离出一株能够高效降解甲醛的假单胞菌(Pseudomonas sp.IOFA1),该菌株可在40 min内完全降解100 mg/cm~3的甲醛.通过阴离子交换柱层析对菌株破碎液中的粗酶液进行纯化,得到一条具有甲醛降解活性的单一蛋白条带.经MALDI-TOF/TOF质谱鉴定,该蛋白为甲醛歧化酶蛋白(FM11).序列分析表明FM11与其它物种已知醛脱氢酶的氨基酸序列最高相似性为65%;结构域分析表明FM11含有2个保守的锌结合位点和一个NAD结合位点.酶学性质分析结果显示,FM11的最适反应温度为40℃,在25~50℃范围内能够保留95%以上的酶活力,尤其是在高于70℃的高温环境下仍保有约50%的活力,表现出广泛的温度适应性.FM11具有非常广泛的最适反应pH值范围(pH值为5~9),且在pH值4和pH值10时仍分别保留50%和80%左右的酶活力,表现出广泛的pH适应性.在pH值为5~7的条件下孵育1 h后,FM11仍保有97%以上的酶活,且在pH值为7~10条件下孵育1 h后,仍保留90%以上的酶活,表现出极好的酸性和碱性稳定性.Ca~(2+)、Mg~(2+)在低浓度时(0.5 mmol/dm~3)对FM11有较明显的激活作用.这些研究为我们进一步开发Pseudomonas sp.IOFA1甲醛歧化酶在降解甲醛方面的用途奠定了基础.  相似文献   

Analysis of phenotypic and genetic parameters for growthrelated traits in the half smooth tongue sole, <Emphasis Type="Italic">Cynoglossus semilaevis</Emphasis>     

刘峰  李仰真  杜民  邵长伟  陈松林 《中国海洋湖沼学报》2016,34(1):163-169

Phenotypic and genetic parameters for growth-related traits in the half-smooth tongue sole, Cynoglossus semilaevis, were estimated in 22 full-sib families produced by normal and neo-male breeding stocks. As phenotypic males with female genotypes, neo-males are harmful in C. semilaevis aquaculture because they reduce overall production. The present study evaluated the difference in the growth-related traits: total length (TL), body weight (BW) and square root of body weight (SQ_BW) at the age of 570 days between normal and neo-male offspring (neo-males used as male parents). The difference in the proportion of females between normal and neo-male offspring was also assessed. Based on the linear mixed model, restricted maximum likelihood (REML) and best linear unbiased prediction (BLUP) were used to estimate various (co)variance components and estimated breeding values (EBVs) of growth-related traits. As a result, all the mean values of the three studied traits were significantly larger in normal offspring than in neo-male offspring. Additionally, the female proportion was significantly larger in normal offspring than in neo-male offspring. Heritability was 0.128±0.066 2 for TL, 0.128±0.065 5 for BW and 0.132±0.062 9 for SQ_BW, all of which were low level heritabilities. The correlation coefficients of EBVs and phenotypic values of the target traits were 0.516 for TL, 0.524 for BW and 0.506 for SQ_BW, all of which were highly significant (P <0.01). Genetic correlations among TL, BW and SQ_BW were positive high (0.921–0.969) and higher than those of phenotype (0.711–0.748), both of which had low standard errors (0.063–0.123 for genotype, and 0.010–0.018 for phenotype). Compared with normal offspring, neo-male offspring have lower breeding values for each studied trait through EBVs comparison. Therefore, neo-male offspring should not be used as broodstock in a C. semilaevis breeding programs.  相似文献   

川西坳陷东坡沙溪庙组气藏成藏演化模式   总被引：1，自引：0，他引：1  

徐敏  刘建  林小云  熊山  牛斌莉 《现代地质》2018,32(5):953

川西坳陷东坡气藏主力产层为沙溪庙组,其圈闭类型以构造-岩性圈闭为主,多为致密砂岩储层,但断层发育,有效改善了储层物性,断砂匹配样式与构造演化对油气成藏过程有重要影响。基于前人已有的认识,通过沙溪庙组气藏成藏动力演化、气水分布特征及生烃期次分析,结合构造演化、成藏幕次等研究,认为沙溪庙组天然气成藏受构造古隆起、断砂配置、储层物性、构造演化影响,形成了“构造控向、断砂控运、储层控藏、演化控调”的成藏演化模式,同中存异,高庙子地区古构造的控制作用更为明显,而中江地区岩性控制作用占主导。沙溪庙组气藏间歇性充注具“燕山期三幕成藏,喜山期调整改造”的成藏特征,多期构造演化和较强的储层非均质性影响了含气饱和度,导致沙溪庙组气藏分布、气藏产能差异大。  相似文献   

Oil geochemistry derived from the Qinjiatun–Qikeshu oilfields: insight from light hydrocarbons     

Jin Li  Min Zhang 《中国地球化学学报》2018,37(3):433-440

A suite of 27 oils from the Qinjiatun–Qikeshu oilfields in the Lishu Fault Depression of the Songliao Basin was analyzed using whole oil gas chromatography. In combination with the relative distribution of C₂₇, C₂₈, and C₂₉ regular steranes, detailed geochemical analyses of light hydrocarbons in oil samples revealed crude oils characterized by the dual input of lower aquatic organisms and higher terrestrial plants. Several light hydrocarbon indicators suggest that the liquid hydrocarbons have maturities equivalent to vitrinite reflectances of around 0.78%–0.93%. This is consistent with the maturity determination of steranes C₂₉ 20S/(20S + 20R) and C₂₉ ααβ/(ααα + αββ). Crude oils derived from the two distinct oilfields likely both have source rocks deposited in a lacustrine environment based on light hydrocarbon parameters and on higher molecular weight hydrocarbon parameters. The results show that light hydrocarbon data in crude oils can provide important information for understanding the geochemical characteristics of the Qinjiatun–Qikeshu oils during geologic evolution.  相似文献   

A New Extension of Geological Work: To Grow Rice in the Desert     

HUANG Min  LIU Zhiqiang  FEI Hongcai  ZHOU Jian  ZHANG Yuxu  HAO Ziguo 《《地质学报》英文版》2018,92(3):1247-1248

正For thousands of years,a large number of peripheral rivers have been flowing into deserts,the water from which then will infiltrate underground or evaporate into the air.When desert sand engulfs and humans retreat,precious freshwater resources lie dormant all the year round,making almost no contribution to human progress.  相似文献   

Far-Field Viscoelastic Post-Seismic Deformation of the 2011 Mw9 Tohoku Earthquake     

Yan hu  Min Wang  Bin Zhao  Kelin Wang 《《地质学报》英文版》2018,92(Z1):163-163

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Structural evolution of a triclinic-type transpressional high strain zone and its role in exhuming the root of the Paleoproterozoic Trans-North China Orogen     

Jian Zhang  Guochun Zhao  Min Sun  Sanzhong Li  Shuwen Liu  Chunjing Wei  Changqing Yin 《《地质学报》英文版》2018,92(Z1):117-117

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坑式钢管静压桩在地基加固中的应用     

周军怀  李向阳  柴卓 《探矿工程》2018,45(11):56-59

坑式钢管静压桩是解决既有建筑物因软弱土层地基产生沉降变形的一种技术方法。针对山西北化关铝化工有限公司质检楼地基沉降变形及工程地质条件,提出了采用坑式钢管静压桩进行加固处理的设计方案,介绍了坑式钢管静压桩施工方案和施工流程及注意事项;总结了施工过程中遇到的问题,并对施工后的监测数据进行了分析。结果表明,坑式钢管静压桩施工对软弱地基加固效果良好,可成功地解决软弱土层产生的地基沉降开裂问题。  相似文献