Evaluation of acetylcholinesterase activity in several zooplanktonic crustaceans     

Minutoli R  Fossi MC  Guglielmo L 《Marine environmental research》2002,54(3-5):799-804

Zooplankton are an essential component of the marine and estuarine food chains. The ecotoxicological risk to zooplanktonic communities, estimated through the use of the biomarkers, can be used as an early warning signal of a potential alteration of the ecosystem health. The aim of this project was to evaluate the potential use of several biomarkers (esterases, mixed function oxidases, porphyrins) in zooplanktonic organisms. The acetylcholinesterase activity (AChE) was determined in homogenates of whole organisms of 10 different zooplanktonic crustaceans. Mean activity of AChE was 10.05 micromol/min/g for Acartia margalefi; 3.30 for Acartia latisetosa; 79.70 for Siriella clausi; 49.97 for Diamysis bahirensis; 7.48 for Siriella armata; 14.20 for Mysidopsis gibbosa; 4.49 for Euphausia crystallorophias; 1.66 for Euphausia superba; 2.74 for Streetsia challengeri; 13.26 for Meganycthiphanes norvegica. The species moreover showed a linear increase in enzyme activity in relation to the increase in sample concentration. The key result of this study concerns the different AChE activity basal values of the crustaceans' different species.  相似文献   

Control over natural resources in ecological economical systems     

I. E. Timchenko  E. M. Igumnova 《Physical Oceanography》2001,11(6):525-541

We consider the problem of stable development of natural economical complexes from the viewpoint of basic balances in an ecological economical system. We construct a basic dynamical model of the complex capable of predicting various scenarios of the industrial development, consumption of natural resources, and pollution of the environment and propose a mechanism of control over the process of development by introducing rent payments for the use of natural resources and ecological fines for the pollution of the environment. The efficiency of the applied model is demonstrated by analysing the process of control over the concentration of biological resources in marine media as an example. We describe the results of numerical experiments performed to show the possibility of creation of the information technology of control over marine bioresources. Translated by Peter V. Malyshev and Dmitry V. Malyshev  相似文献   

An aromatase inhibitor and tamoxifen decrease plasma levels of o,p'-DDT and its metabolites in Nile tilapia (Oreochromis niloticus)     

Leaños-Castañeda O  Gold-Bouchot G  Van Der Kraak G  Lister A  Ceja-Moreno V  Simá-Alvarez R 《Marine environmental research》2004,58(2-5):337-342

The objective of this study was to determine if tamoxifen or an aromatase inhibitor (4-hydroxyandrostenedione; 4-OHA) affected plasma concentrations of o,p' -DDT and its metabolites, o,p'-DDD and o,p'-DDE, in mature tilapia. Male and female tilapia were injected 6 times intraperitoneally with o,p'-DDT (40 mg/kg) alone or in combination with 4-OHA (2 mg/kg) over a 12 day period. An additional group of male fish was injected with tamoxifen (5 mg/kg) plus o,p'-DDT. At the end of the treatment period, plasma samples were extracted and analyzed by GC/ECD. Females injected only with o,p'-DDT had significantly higher levels of o,p'-DDT compared with males. Interestingly, females and males treated concomitantly with o,p'-DDT and 4-OHA or tamoxifen had significantly lower concentrations of plasma o,p'-DDT (about 50%) compared with fish treated with only o,p'-DDT. These initial results suggest that an interaction between endocrine-active compounds occurs in vivo in tilapia and may involve alterations in metabolism of o,p'-DDT.  相似文献   

Evaluation of toxicity in tributaries of the Mersey estuary using the isopod Asellus aquaticus (L.)     

A. J. O'Neill  T. S. Galloway  M. A. Browne  A. Dissanayake  M. H. Depledge 《Marine environmental research》2004,58(2-5):327

Increasing numbers of industrial, agricultural and natural chemicals are present in sewage effluent and are known to elicit toxic effects in laboratory exposures, but little is known of their combined sub-lethal effect in the field. In this study, a combination of esterase activity and ventilation rate assays was performed to determine the neurological and physiological function of the freshwater crustacean Asellus aquaticus (L.) at sites above and below a sewage treatment works (STW). Cholinesterase and carboxylesterase activities were significantly inhibited (n=8, P<0.05) and ventilation rates increased (n=8, P=0.0001) in A. aquaticus at STW sites compared to those from reference sites, indicating a decrease in neurological and physiological function. The ecological relevance of these findings for the population dynamics of the organisms in the field is discussed.  相似文献   

Modelling of flow around a near-bed pipeline with a spoiler   总被引：1，自引：0，他引：1  

L. Cheng  L. W. Chew 《Ocean Engineering》2003,30(13):1595-1611

Flow around a pipeline with and without a spoiler near a smooth wall is simulated by solving the Navier–Stokes equations. Finite-difference formulation with a second-order upwind scheme in a curvilinear coordinate system is employed. The influences of the spoiler on hydrodynamic forces, pressure distribution, vortex shedding frequency, velocity profile under the pipe, as well as shear stress on the wall are investigated. The attachment of a spoiler significantly increases drag, root-mean-square (RMS) lift, flow through the gap between the pipe and the wall and shear stress on the seabed around the pipe. The spoiler also generates a non-zero mean downward force on the pipeline, which may enhance the self-burial of the pipeline.  相似文献   

Continuous Plankton Recorder sampling off the east coast of North America: history and status     

J. W. Jossi  A. W. G. John  D. Sameoto 《Progress in Oceanography》2003,58(2-4):313

The Continuous Plankton Recorder (CPR) survey has sampled plankton on 14 routes off the coasts of the northeast United States and Canada between 1959 and 2000. Six of these routes are still operating and are sampled on a monthly basis. Some 2047 CPR tows have been made to the end of 2000 and the resulting database represents the most extensive time series of marine plankton available anywhere in the northwest Atlantic. The location and time span of coverage of each route is presented. In addition selected information is presented on:

1. zooplankton abundance as departures from baselines for the northeast US continental shelf;

2. interannual variation in seasonality of Gulf of Maine phytoplankton;

3. zooplankton relationships to local hydrography of the Gulf of Maine and to the North Atlantic Oscillation;

4. time-space matrices of zooplankton abundance and anomalies southeast of New York City;

5. time series of phyto- and zooplankton on the Scotian Shelf;

6. seasonal cycles of Phytoplankton Colour and of zooplankton on the Scotian Shelf and Georges Bank, and in the Gulf of Maine; and

7. monthly abundance of zooplankton in Narragansett Bay, Rhode Island.

  相似文献   

Sources and distribution of organic matter in a river-dominated estuary (Winyah Bay, SC, USA)   总被引：12，自引：0，他引：12  

Miguel A. Goi  Maria J. Teixeira  David W. Perkey 《Estuarine, Coastal and Shelf Science》2003,57(5-6):1023-1048

The sources and distribution of organic matter (OM) in surface waters and sediments from Winyah Bay (South Carolina, USA) were investigated using a variety of analytical techniques, including elemental, stable isotope and organic biomarker analyses. Several locations along the estuary salinity gradient were sampled during four different periods of contrasting river discharge and tidal range. The dissolved organic carbon (DOC) concentrations of surface waters ranged from 7 mg l⁻¹ in the lower bay stations closest to the ocean to 20 mg l⁻¹ in the river and upper bay samples. There was a general linear relationship between DOC concentrations and salinity in three of the four sampling periods. In contrast, particulate organic carbon (POC) concentrations were significantly lower (0.1–3 mg l⁻¹) and showed no relationship with salinity. The high molecular weight dissolved OM (HMW DOM) isolated from selected water samples collected along the bay displayed atomic carbon:nitrogen ratios ([C/N]a) and stable carbon isotopic compositions of organic carbon (δ¹³C_OC) that ranged from 10 to 30 and from −28 to −25‰, respectively. Combined, such compositions indicate that in most HMW DOM samples, the majority of the OM originates from terrigenous sources, with smaller contributions from riverine and estuarine phytoplankton. In contrast, the [C/N]a ratios of particulate OM (POM) samples varied significantly among the collection periods, ranging from low values of 5 to high values of >20. Overall, the trends in [C/N]a ratios indicated that algal sources of POM were most important during the early and late summer, whereas terrigenous sources dominated in the winter and early spring.In Winyah Bay bottom sediments, the concentrations of the mineral-associated OM were positively correlated with sediment surface area. The [C/N]a ratios and δ¹³C_OC compositions of the bulk sedimentary OM ranged from 5 to 45 and from −28 to −23‰, respectively. These compositions were consistent with predominant contributions of terrigenous sources and lesser (but significant) inputs of freshwater, estuarine and marine phytoplankton. The highest terrigenous contents were found in sediments from the river and upper bay sites, with smaller contributions to the lower parts of the estuary. The yields of lignin-derived CuO oxidation products from Winyah Bay sediments indicated that the terrigenous OM in these samples was composed of variable mixtures of relatively fresh vascular plant detritus and moderately altered soil OM. Based on the lignin phenol compositions, most of this material appeared to be derived from angiosperm and gymnosperm vascular plant sources similar to those found in the upland coastal forests in this region. A few samples displayed lignin compositions that suggested a more significant contribution from marsh C₃ grasses. However, there was no evidence of inputs of Spartina alterniflora (a C₄ grass) remains from the salt marshes that surround the lower sections of Winyah Bay.  相似文献   

Upstream dams and downstream clams: growth rates of bivalve mollusks unveil impact of river management on estuarine ecosystems (Colorado River Delta, Mexico)     

B. R. Schne  K. W. Flessa  D. L. Dettman  D. H. Goodwin 《Estuarine, Coastal and Shelf Science》2003,58(4):715-726

We studied how the extensive diversion of Colorado River water, induced by dams and agricultural activities of the last 70 years, affected the growth rates of two abundant bivalve mollusk species (Chione cortezi and Chione fluctifraga) in the northern Gulf of California. Shells alive on the delta today (‘Post-dam’ shells) grow 5.8–27.9% faster than shells alive prior to the construction of dams (‘Pre-dam’ shells). This increase in annual shell production is linked to the currently sharply reduced freshwater influx to the Colorado River estuary. Before the upstream river management, lower salinity retarded growth rates in these bivalves. Intra-annual growth rates were 50% lower during spring and early summer, when river flow was at its maximum. Growth rates in Chione today are largely controlled by temperature and nutrients; prior to the construction of dams and the diversion of the Colorado River flow, seasonal changes in salinity played an important role in regulating calcification rates.Our study employs sclerochronological (growth increment analysis) and geochemical techniques to assess the impact of reduced freshwater influx on bivalve growth rates in the Colorado River estuary. A combination of both techniques provides an excellent tool to evaluate the impact of river management in areas where no pre-impact studies were made.  相似文献   

两株琼胶酶高产细菌的筛选和鉴定   总被引：4，自引：1，他引：4  

杜宗军  王鹏  李筠  P.A.W.Robertson  B.Austin 《海洋科学》2002,26(3):1-4

从海洋环境中筛选得到两株琼胶酶的高产菌株，通过形态观察和生理生化反应，确定它们属于弧菌属，通过BIOLOG细菌鉴定系统鉴定，并同弧菌属标准菌株分析比较，确定上方宝剑两株菌都是塔式弧菌（Vibrio tubiashii)，这两株菌在碳源利用方面存在差别。  相似文献   

Esterases as biomarkers in Nereis (Hediste) diversicolor exposed to temephos and Bacillus thuringiensis var. israelensis used for mosquito control in coastal wetlands of Morbihan (Brittany,France)     

Fourcy D  Jumel A  Heydorff M  Lagadic L 《Marine environmental research》2002,54(3-5):755-759

Since 1998, a biomonitoring programme has been implemented to assess the potential impact of chemical mosquito control on macroinvertebrates of the coastal wetlands of Morbihan (Brittany, France). Acetylcholinesterase and carboxylesterases were used as biomarkers to assess the effects of Abate 500e (a.i. temephos) and Vectobac 12 AS (a.i. endotoxins of Bacillus thuringiensis var. israelensis, Bti) in Nereis (Hediste) diversicolor. Esterase inhibition revealed a marked impact of temephos, suggesting preferential contamination of the worms through the food. In Bti-exposed N. diversicolor, random variations of esterase activities were observed, that could not be attributed to the larvicide. However, esterases only reflected indirect physiological effects of Bti, and further investigations are needed to identify biomarkers more specific of Bti endotoxins.  相似文献