裙带菜配子体基因工程选择标记的研究   总被引：1，自引：1，他引：0  

张亦陈  高江涛  张喆  姜鹏  秦松 《海洋科学》2007,31(12):64-68

研究了裙带菜(Undaria pinnatifida)配子体对除草剂草丁膦的敏感性以及温度对草丁膦处理效果的影响。结果表明,裙带菜配子体对草丁膦敏感;统计学分析得出了草丁膦在不同温度条件下处理裙带菜配子体的半致死浓度(LD50)及95%可信限;温差达到10℃时,相同处理时间各组内不同处理温度样品的LD50之间呈现显著差异,第6天的LD50由10℃组的15.78 mg/L降为20℃组的11.56 mg/L和25℃组的10.33 mg/L。研究发现可以尝试采用草丁膦作为裙带菜配子体遗传转化的选择试剂。  相似文献   

山东蓬莱大黑山岛碱性火山岩地球化学特征     

黄朋  付永涛  李安春  肖尚斌 《海洋与湖沼》2007,38(1):91-96

庙岛群岛中的大黑山岛出露碱性火山岩,对研究壳-幔过程及黄、渤海演化具重要意义。文中作者对大黑山岛火山岩的常、微量元素、K-Ar年龄及Nd-Sr-Pb同位素体系进行测试确定。结果表明,岩石属钠质碱性玄武岩系列,岩浆岩喷发可分为8Ma前和8Ma后两个阶段;岩石常量元素含量除K2O、Na2O外差异小;随着火山岩的演化,岩浆源区深度变大;大黑山火山岩Nd-Sr-Pb同位素比值与夏威夷洋岛玄武岩相似,其岩浆的地幔端元组分为PREMA DM,又以PREMA为主,并有MORB物质混入。  相似文献   

Using otolith weight to predict the age of <Emphasis Type="Italic">Pennahia macrocephalus</Emphasis> in the mouth of the Beibu Gulf     

颜云榕  吴艳芳  卢伙胜  李忠炉  金显仕 《中国海洋湖沼学报》2009,27(2):342-349

The relation between otolith weight (OW) and the age of marine fish is studied. A total of 222 individuals of bighead white croaker, Pennahia macrocephalus were sampled seasonally in the mouth of the Beibu Gulf, the South China Sea, in 2007. Since there are no significant differences in sagittal OW between otolith in pairs (P≥0.05), the undamaged left sagittal otolith is used for age determination. The highest correlations among standard length, OW and fish ages are confirmed by linear, exponential and multinomial regression. Results show that sagittal OW overlaps only occasionally among age groups, and to individuals with similar standard length, the older and slower-growing fish has a heavier otolith because of the continued otolith material deposition. There are differences in sagittal OW among different age groups and significant positive linear relationship with age (P<0.05). The age readings can be verified by plotting the sagittal OW versus the standard length for age groups, and the individuals with similar standard length but in different ages can be separated by sagittal OW frequency analysis. Mostly, the predicted ages using the regression between sagittal OW and ages are closed to the observed ages by counting annulus on scale. It indicates that the sagittal OW analysis is a useful technique for validating the accuracy of age determination by annuli counts, especially for individuals of similar size. Furthermore, the technique is applied for Pennahia macrocephalus with discussion in this paper. Supported by the National Natural Science Foundation of China (No. 30771653) and Bureau of Fisheries, Ministry of Agriculture  相似文献   

Characterization of a new lectin involved in the protoplast regeneration of <Emphasis Type="Italic">Bryopsis hypnoides</Emphasis>     

牛建峰  王广策  吕芳  周百成  彭光 《中国海洋湖沼学报》2009,27(3):502-512

A group of coenocytic marine algae differs from higher plants,whose totipotency depends on an intact cell(or protoplast).Instead,this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals.It is thought that lectins play a key role in the aggregation process.We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides.The lectin was ca.27 kDa with a pI between pH 5 and pH 6.The absence of carbohydrate suggested that the lectin was ...  相似文献   

Impacts of soil fauna on litter decomposition at different succession stages of wetland in Sanjiang Plain, China   总被引：1，自引：0，他引：1  

Haitao Wu  Xianguo Lu  Ming Jiang  Xiao Bao 《中国地理科学(英文版)》2009,19(3):258-264

Litter decomposition is the key process in nutrient recycling and energy flow. The present study examined the impacts of soil fauna on decomposition rates and nutrient fluxes at three succession stages of wetland in the Sanjiang Plain, China using different mesh litterbags. The results show that in each succession stage of wetland, soil fauna can obviously increase litter decomposition rates. The average contribution of whole soil fauna to litter mass loss was 35.35%. The more complex the soil fauna group, the more significant the role of soil fauna. The average loss of three types of litter in the 4mm mesh litterbags was 0.3–4.1 times that in 0.058mm ones. The decomposition function of soil fauna to litter mass changed with the wetland succession. The average contribution of soil fauna to litter loss firstly decreased from 34.96% (Carex lasiocapa) to 32.94% (Carex meyeriana), then increased to 38.16% (Calamagrostics angustifolia). The contributions of soil fauna to litter decomposition rates vary according to the litter substrata, soil fauna communities and seasons. Significant effects were respectively found in August and July on C. angustifolia and C. lasiocapa, while in June and August on C. meyeriana. Total carbon (TC), total nitrogen (TN) and total phosphorus (TP) contents and the C/N and C/P ratios of decaying litter can be influenced by soil fauna. At different wetland succession stages, the effects of soil fauna on nutrient elements also differ greatly, which shows the significant difference of influencing element types and degrees. Soil fauna communities strongly influenced the TC and TP concentrations of C. meyeriana litter, and TP content of C. lasiocapa. Our results indicate that soil fauna have important effects on litter decomposition and this influence will vary with the wetland succession and seasonal variation. Foundation item: Under the auspices of State Key Development Program for Basic Research of China (No. 2009CB421103), Key Program of National Natural Science Foundation of China (No. 40830535/D0101), Knowledge Innovation Programs of Chinese Academy of Sciences (No. KZCX2-YW-BR-16, KSCX2-YW-N-46-06)  相似文献   

Distribution of the nonlinear random ocean wave period     

侯一筠  李明杰  宋贵霆  司广成  齐鹏  胡珀 《中国海洋湖沼学报》2009,27(4):919-923

Because of the intrinsic difficulty in determining distributions for wave periods, previous studies on wave period distribution models have not taken nonlinearity into account and have not performed well in terms of describing and statistically analyzing the probability density distribution of ocean waves. In this study, a statistical model of random waves is developed using Stokes wave theory of water wave dynamics. In addition, a new nonlinear probability distribution function for the wave period is presented with the parameters of spectral density width and nonlinear wave steepness, which is more reasonable as a physical mechanism. The magnitude of wave steepness determines the intensity of the nonlinear effect, while the spectral width only changes the energy distribution. The wave steepness is found to be an important parameter in terms of not only dynamics but also statistics. The value of wave steepness reflects the degree that the wave period distribution skews from the Cauchy distribution, and it also describes the variation in the distribution function, which resembles that of the wave surface elevation distribution and wave height distribution. We found that the distribution curves skew leftward and upward as the wave steepness increases. The wave period observations for the SZFII-1 buoy, made off the coast of Weihai (37°27.6′ N, 122°15.1′ E), China, are used to verify the new distribution. The coefficient of the correlation between the new distribution and the buoy data at different spectral widths (ν=0.3−0.5) is within the range of 0.968 6 to 0.991 7. In addition, the Longuet-Higgins (1975) and Sun (1988) distributions and the new distribution presented in this work are compared. The validations and comparisons indicate that the new nonlinear probability density distribution fits the buoy measurements better than the Longuet-Higgins and Sun distributions do. We believe that adoption of the new wave period distribution would improve traditional statistical wave theory.  相似文献   

The selection of alkaline protease-producing yeasts fi＇om marine environments and evaluation of their bioactive peptide production     

李静  池振明  王祥红  彭莹  池哲 《中国海洋湖沼学报》2009,27(4):753-761

A total of 400 yeast strains from seawater, sediments, saltern mud, marine fish guts, and marine algae were obtained. The protease activity of the yeast cultures was estimated, after which four strains (HN3.11, N11b, YF04C and HN4.9) capable of secreting extracellular alkaline protease were isolated. The isolated strains were identified as Aureobasidium pullulans, Yarrowia lipolytica, Issatchenkia orientalis and Cryptococcus cf. aureus. The optimal pH of the protease activity produced by strains HN3.11, YF04C, and HN4.9 was 9.0, while that of the protease produced by strain N11b was 10.0. The optimal temperature for protease activity was 45°C for strains HN3.11, N11b, and YF04C, and 50°C for strain HN4.9. After digestion of shrimp (Penaeus vannamei) protein and spirulina (Arthospira platensis) protein with the four crude alkaline proteases, the filtrate from spirulina (Arthrospira platensis) powder digested by the crude alkaline protease of strain HN3.11 was found to have the highest antioxidant activity (61.4%) and the highest angiotensin I converting enzyme (ACE)-inhibitory activities (68.4%). The other filtrates had much lower antioxidant activity and ACE-inhibitory activities.  相似文献   

Ecosystem health assessment of Honghu Lake Wetland of China using artificial neural network approach   总被引：3，自引：0，他引：3  

Minghao Mo  Xuelei Wang  Houjian Wu  Shuming Cai  Xiaoyang Zhang  Huiliang Wang 《中国地理科学(英文版)》2009,19(4):349-356

Honghu Lake, located in the southeast of Hubei Province, China, has suffered a severe disturbance during the past few decades. To restore the ecosystem, the Honghu Lake Wetland Protection and Restoration Demonstration Project (HLWPRDP) has been implemented since 2004. A back propagation (BP) artificial neural network (ANN) approach was applied to evaluatinig the ecosystem health of the Honghu Lake wetland. And the effectiveness of the HLWPRDP was also assessed by comparing the ecosystem health before and after the project. Particularly, 12 ecosystem health indices were used as evaluation parameters to establish a set of three-layer BP ANNs. The output is one layer of ecosystem health index. After training and testing the BP ANNs, an optimal model of BP ANNs was selected to assess the ecosystem health of the Honghu Lake wetland. The result indicates that four stages can be identified based on the change of the ecosystem health from 1990 to 2008 and the ecosystem health index ranges from morbidity before the implementation of HLWPRDP (in 2002) to middle health after the implementation of the HLWPRDP (in 2005). It demonstrates that the HLWPRDP is effective and the BP ANN could be used as a tool for the assessment of ecosystem health.  相似文献   

Livelihood strategy change and land use change —Case of Danzam Village in upper Dadu River watershed, Tibetan Plateau of China   总被引：2，自引：1，他引：1  

Jianzhong Yan  Yili Zhang  Liping Zhang  Yingying Wu 《中国地理科学(英文版)》2009,19(3):231-240

Land use change in rural China since the 1980s, induced by institution reforms, urbanization, industrialization and population increase, has received more attention. However, case studies on how institution reforms affect farmers’ livelihood strategies and drive land use change are scarce. By means of cropland plots investigations and interviews with farmers, this study examines livelihood strategy change and land use change in Danzam Village of Jinchuan County in the upper Dadu River watershed, eastern Tibetan Plateau, China. The results show that, during the collective system period, as surplus labor forces could not be transferred to the secondary and tertiary industries, they had to choose agricultural involution as their livelihood strategy, then the farmers had to produce more grains by land reclamation, increasing multiple cropping index, improving input of labor, fertilizer, pesticide and adopting advanced agricultural techniques. During the household responsibility system period, as labors being transferred to the secondary and tertiary industries, farmers chose livelihood diversification strategy. Therefore, labor input to grain planting was greatly reduced, which drove the transformation of grain to horticulture, vegetable or wasteland and decrease of multiple cropping index. This study provides a new insight into understanding linkages among institution reforms, livelihood strategy of smallholders and land use change in rural China. Foundation item: Under the auspices of National Natural Science Foundation of China (No. 40601006, 40471009), National Basic Research Program of China (No. 2005CB422006)  相似文献   

浅论县（市）级土地利用数据库系统建设     

纪玉国  吴汉涛  隋晓英 《山东国土资源》2009,25(7):5-6

随着经济的迅猛发展,各地土地利用情况多种多样,采用手工管理土地资料更新周期长,综合利用率低,各种查询统计要耗费大量的人力物力,而且统计计算的数据结果精度不高。因此仅仅依靠传统的手工管理模式已经远远不能满足土地管理工作的需要,为保质保量按时完成全国土地利用现状更新调查,加强地籍规范化管理,利用成熟的计算机技术辅助进行土地调查已势在必行。  相似文献