cDNA cloning of an aryl hydrocarbon receptor from Baikal seals (Phoca sibirica)     

Kim EY  Hahn ME  Iwata H  Tanabe S  Miyazaki N 《Marine environmental research》2002,54(3-5):285-289

Species differences in sensitivity to related planar halogenated aromatic hydrocarbons (PHAH) add significant uncertainty in assessing the ecological risk to aquatic mammals. To investigate mechanisms of PHAH sensitivity in aquatic mammals, we cloned and sequenced the cDNA of Baikal seal aryl hydrocarbon receptor (AHR), an intracellular protein that initiates PHAH-mediated effects. The Baikal seal AHR cDNA has an open reading frame of 843 amino acid residues with a predicted molecular mass of 94.6 kDa. Comparison of AHR amino acid sequences indicated a high degree of sequence conservation (98%) between Baikal and harbor seals. The high conservation of AHRs between Baikal and harbor seals indicates that these seals express AHR proteins closely related structurally. In our previous report (Kim & Hahn, 2002), the dioxin-binding affinity of the harbor seal AHR was at least as high as that of the AHR from a dioxin-sensitive strain of mice, suggesting that this seal species may be sensitive to PHAH effects. This implies that Baikal seal may also be sensitive to dioxin effects.  相似文献   

Measurements of the Turbulent Energy Dissipation Rate around the Shelf Break in the East China Sea   总被引：1，自引：0，他引：1  

Takeshi?MatsunoEmail author  Manabu?Shimizu  Yasuhiro?Morii  Hideaki?Nishida  Yasuaki?Takaki 《Journal of Oceanography》2005,61(6):1029-1037

Using the micro-structure profiler, TurboMAP, large values for the turbulent energy dissipation rate ε were found just above the bottom of the shelf and around the thermocline near the continental shelf break in the East China Sea. The values found above the bottom are produced by the bottom stress due to tidal currents, resulting in a distinct bottom mixed layer where the vertical eddy diffusivity Kz is also large. Distinct maxima in the values of ε detected around the thermocline are located at the depth of the fine-scale shear maxima detected with the moored ADCP. The vertical profiles of ε were compared with those of the current velocity, and it was found that the maxima in ε appear to correspond to those of the shear with fine scale. The magnitude of the observed ε coincided approximately with the ε calculated from the fine-scale shear and the buoyancy frequency according to the parameterization proposed by Gregg (1989), if the large-scale mean shear caused by the Kuroshio is subtracted. However, it is not clear whether the parameterization for the internal wave fields in the open ocean is applicable to the estimation of ε in the shelf break. Whereas the most predominant value of ε was found just above the bottom and around the thermocline, the maxima of ε could be found in the internal area. They could have been caused by the propagation of the vertically high wave number internal tides along the characteristic ray.  相似文献   

Mathematical model of fish schooling behaviour in a set-net     

Takagi  Tsutomu; Moritomi  Yutaka; Iwata  Jyun; Nakamine  Hiroshi; Sannomiya  Nobuo 《ICES Journal of Marine Science》2004,61(7):1214-1223

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Low detection capability of global earthquakes after the occurrence of large earthquakes: investigation of the Harvard CMT catalogue   总被引：4，自引：0，他引：4  

Takaki Iwata 《Geophysical Journal International》2008,174(3):849-856

We investigated the detection capability of global earthquakes immediately after the occurrence of a large earthquake. We stacked global earthquake sequences after occurrences of large earthquakes obtained from the Harvard centroid-moment tensor catalogue, and applied a statistical model that represents an observed magnitude–frequency distribution of earthquakes to the stacked sequence. The temporal variation in model parameters, which corresponds to the detection capability of earthquakes, was estimated using a Bayesian approach. We found that the detection capability of global earthquakes is lower than normal for several hours after the occurrence of large earthquakes; for instance, the duration of lowered detection capability of global earthquakes after the occurrence of an earthquake with a magnitude of seven or larger is estimated to be approximately 12 hr.  相似文献   

Calculating downward longwave radiation under clear and cloudy conditions over a tropical lowland forest site: an evaluation of model schemes for hourly data     

Toby R. Marthews  Yadvinder Malhi  Hiroki Iwata 《Theoretical and Applied Climatology》2012,107(3-4):461-477

Field measurements of radiation fluxes—notably downwelling longwave radiation flux (LW flux)—are as yet rare or nonexistent outside a very select number of sites in the tropics. Data gaps can only be filled through the use of estimation schemes based on measurements of other meteorological variables, and there is a need for recommendations on best practice in this area. We selected 18 contrasting semi-empirical estimation schemes for downward longwave radiation, based on air emissivities, combined with six different sky cover estimation schemes and compared the expected longwave flux with hourly observations from a flux tower at Caxiuan? in Brazil. Of all schemes tested, the Dilley–Kimball emissivity scheme combined with Kasten and Czeplak’s sky cover scheme during the day and Dilley and O’Brien’s model B scheme at night proved to be the most reliable, yielding estimates of LW flux generally within 20?W/m² of measurements across all time points.  相似文献   

Arsenic species and their accumulation features in green turtles (Chelonia mydas)     

Tetsuro Agusa  Kozue Takagi  Hisato Iwata  Shinsuke Tanabe   《Marine pollution bulletin》2008,57(6-12):782

Total arsenic (As) and its compounds were determined in liver, kidney, muscle, and stomach contents of green turtles (Chelonia mydas). Total As concentrations in the muscle were higher than those in the kidney and liver. Arsenobetaine (AB) was the predominant compound in all the three tissues and its levels were positively correlated with total As concentrations. This indicates that AB greatly contributes to As accumulation in green turtles. Higher concentrations of remaining As in the sample after extraction were detected in the liver, implying that lipid-soluble or protein bound As compounds accumulate in the liver of green turtles. Total As levels in tissues showed significant negative correlations with standard carapace length. The size-dependence of As accumulation in green turtles may be related to their feeding habit, shifting from carnivore to herbivore at different growth stages. Concentrations of AB and dimethylarsinic acid (DMA) were low in the stomach contents but high in the tissues, implying bioaccumulation of these arsenicals in green turtles.  相似文献   

Toxicogenomic analysis of immune system-related genes in Japanese flounder (Paralichthys olivaceus) exposed to heavy oil   总被引：1，自引：1，他引：0  

Kei Nakayama  Shin-Ichi Kitamura  Yasunori Murakami  Jun-Young Song  Sung-Ju Jung  Myung-Joo Oh  Hisato Iwata  Shinsuke Tanabe 《Marine pollution bulletin》2008,57(6-12):445

Heavy oil contamination is one of the most important environmental issues. Toxicities of polycyclic aromatic hydrocarbons (PAHs), including immune toxicities, are well characterized, however, the immune toxic effects of heavy oil, as a complex mixture of PAHs, have not been investigated. In the present study, we selected Japanese flounder (Paralichthys olivaceus) as a model organism, and observed alteration of immune function by the exposure to heavy oil. To analyze the expression profiles of immune system-related genes, we selected 309 cDNAs from our flounder EST library, and spotted them on a glass slide. Using this cDNA array, alteration of gene expression profiles was analyzed in the kidneys of flounders exposed to heavy oil. Six Japanese flounders (mean body weight: 197 g) were acclimated to laboratory conditions at 19–20 °C. Three fish were exposed to heavy oil C (bunker C) at a concentration of 3.8 g/L for 3 days, and the others were kept in seawater without heavy oil and used as the control. After the exposure period, the fish were transferred into control seawater and maintained for 4 days, and then they were dissected and their kidneys were removed. Total RNA was extracted from the kidney samples to use in gene expression analyses. The microarray detected alteration of immune system-related genes in the kidneys of heavy oil-exposed flounders, including down-regulation of immunoglobulin light chain, CD45, major histocompatibility complex class II antigens and macrophage colony-stimulating factor precursor, and up-regulation of interleukin-8 and lysozyme. These results suggest that pathogen resistance may be weakened in heavy oil-exposed fish, causing a subsequent bacterial infection, and then proinflammatory genes may be induced as a defensive response against the infection. Additionally, we found candidate genes for use as biomarkers of heavy oil exposure, such as N-myc downstream regulated gene 1 and heat shock cognate 71 kDa proteins.  相似文献   

Investigation of influence of tunneling on existing building and tunnel: model tests and numerical simulations     

Hossain Md. Shahin  Teruo Nakai  Kenji Ishii  Toshikazu Iwata  Shou Kuroi 《Acta Geotechnica》2016,11(3):679-692

To investigate the realistic ground behavior during tunneling, a new device has been developed. With the new device, model tests of tunnel excavation considering an existing tunnel and an existing building were carried out. Non-linear finite element analyses corresponding to the model tests were also conducted using FEMtij-2D software where an elastoplastic subloading t _ij model was used to describe the mechanical behavior of soil. Earth pressure distribution around the tunnels and ground movements during tunnel excavation depend on the distance and position between the twin tunnels. There is a significant effect of tunneling on the existing foundation of building even in the case where the tunnel is constructed in deep underground. The numerical analyses capture well the results of the model tests.  相似文献   

Modelling of ground motion in the Higashinada (Kobe) area for an aftershock of the 1995 January 17 Hyogo-ken Nanbu, Japan, earthquake     

Arben Pitarka  Kojiro Irikura  Tomotaka Iwata 《Geophysical Journal International》1997,131(2):231-239

We model the ground motion from an aftershock of the 1995 January 17 Hyogo-ken Nanbu (Kobe) earthquake to investigate basin edge effects on wave propagation in Higashinada ward, downtown Kobe. Point-source finite-difference seismograms calculated using a double-couple solution and a 2-D basin structure are compared with the ground-motion velocity seismograms recorded in a small station array deployed at sites within and outside the heavily damage zone in Higashinada ward. The comparison suggests that in the frequency range 0.1-2 Hz that was analysed, the observed spatial amplitude variation of the aftershock ground motion is attributable mainly to the basin edge effects. We found that the basin edge effect, caused by the superposition of the direct S wave and the basin-edge-diffracted waves, amplified the ground motion in a narrow zone that is offset by about 0.7 km from the basin edge.  相似文献   

Transformation of the Indonesian Throughflow Water by Vertical Mixing and Its Relation to Tidally Generated Internal Waves     

Takaki Hatayama 《Journal of Oceanography》2004,60(3):569-585

Numerical experiments with two-dimensional nonhydrostatic model have been performed to investigate tidally generated internal waves at the Dewakang sill at the southern Makassar Strait where two large-amplitude “bumps” of relatively shallow water exist. We investigate the effect of these features on vertical mixing, with emphasis on the transformation of the Indonesian throughflow (ITF) water properties. The result shows that large-amplitude internal waves are generated at both bumps by the predominant M₂ tidal flow, even though the condition of the critical Froude number and the critical slope are not satisfied. The internal waves induce such vigorous vertical mixing in the sill region that the vertical diffusivity attains a maximum value of 6 × 10⁻³ m²s⁻¹ and the salinity maximum and minimum core layers characterizing the ITF thermocline water are considerably weakened. Close examination reveals that bottom-intensified currents produced mainly by the joint effect of barotropic M₂ flow and internal tides generated in the concave region surrounding both bumps can excite unsteady lee waves (Nakamura et al., 2000) on the inside slopes of the bumps, which tend to be trapped at the generation region and grow into large-amplitude waves. Such generation of unsteady lee waves does not occur in case of one bump alone. Trapping and amplification of the waves in the sill region induce large vertical displacements (∼60 m) of water parcels during one tidal period, leading to strong vertical mixing there. Since the K₁ tidal currents are relatively weak, large-amplitude internal waves causing intense vertical mixing are not generated. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献