Isolation of protein from Chlorella sorokiniana CY1 using liquid biphasic flotation assisted with sonication through sugaring-out effect     

Chia  Shir Reen  Chew  Kit Wayne  Show  Pau Loke  Sivakumar  Manickam  Ling  Tau Chuan  Tao  Yang 《中国海洋湖沼学报》2019,37(3):898-908

Microalgae, a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical, bioenergy and food applications. This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga, Chlorella sorokiniana CY1 which was assisted with sonication. A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides, glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein). The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases, as the yield of protein increased to 77%. The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well. The optimum protein productivity was obtained with 10 s of resting time in between sonication. Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode. The optimum condition for protein extraction were found as followed: 200 g/L glucose as bottom phase with volume ratio of I:1.25, 10 s of resting time for ultrasonication, 5 s of ultrasonication in pulse mode and 0.25 g of biomass weight. The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.  相似文献   

Hydrologist: John D. Hewlett (1922–2004)     

C. Rhett Jackson  Wayne T. Swank  Rob Olszewski 《水文研究》2005,19(10):2093-2095

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Ammonium excretion by benthic invertebrates and sediment-water nitrogen flux in the Gulf of Mexico near the Mississippi River outflow   总被引：1，自引：0，他引：1  

Wayne S. Gardner  Elva Escobar Briones  Elizabeth Cruz Kaegi  Gilbert T. Rowe 《Estuaries and Coasts》1993,16(4):799-808

Benthic macroinvertebrate biomass and ammonium excretion rates were measured at four stations in the Gulf of Mexico near the Mississippi River mouth. Calculated areal excretion rates were then compared to sediment-water nitrogen fluxes measured in benthic bottom lander chambers at similar stations to estimate the potential importance of macroinvertebrate excretion to sediment nitrogen mineralization. Excretion rates for individual crustaceans (amphipods and decapods) was 2–21 nmoles NH₄ ⁺ (mg dry weight)^?1 h^?1. The mean excretion rates for the polychaetes, Paraprionaspio pinnata [6–12 nmoles NH₄ ⁺ (mg dry weight)^?1h^?1] and Magelona sp. [27–53 nmoles NH₄ ⁺ (mg dry weight)^?1h^?1], were comparable or higher than previous measurements for similar size benthic or pelagic invertebrates incubated at the same temperature (22±1°C). Although the relatively high rates of excretion by these selective feeders may have been partially caused by experimental handling effects (e.g., removal from sediment substrates), they probably reflected the availability of nitrogen-rich food supplies in the Mississippi River plume. When the measured weight-specific rates were extrapolated to total areal biomass, areal macroinvertebrate excretion estimates ranged from 7 μmole NH₄ ⁺ m^?2h^?1 at a 40-m deep station near the river mouth to 18 μmole NH₄ ⁺ m^?2h^?1 at a shallower (28-m deep) station further from the river mouth. The net flux of ammonium and nitrate from the sediments to the water measured in bottom lander chambers in the same region were 15–53 μmole NH₄ ⁺ m^?2h^?1 and ?25–21 μmole NO₃ ^? m^?2h^?1. These results suggest that excretion of NH₄ ⁺ by macroinvertebrates could be a potentially important component of benthic nitrogen regeneration in the Mississippi River plume-Gulf shelf region.  相似文献   

Boundary-layer depth and entrainment zone characterization with a boundary-layer profiler   总被引：13，自引：0，他引：13  

Wayne M. Angevine  Allen B. White  S. K. Avery 《Boundary-Layer Meteorology》1994,68(4):375-385

A technique for determining the height of the convective atmospheric boundary layer (CBL) with a 915 MHz boundary-layer profiler is discussed. The results are compared with CBL heights determined from radiosonde measurements. The profiler provides continuous CBL height measurements with very good time resolution (30 minutes or less), allowing for detailed understanding of the growth and fluctuations of the CBL. In addition, the profiler provides information about the degree of definition of the CBL top and the thickness of the entrainment zone. The measurements discussed were taken during the Rural Oxidants in the Southern Environment II (ROSE II) experiment.  相似文献   

Solution of H2O in diopside melts: a thermodynamic model     

David H. Eggler  C. Wayne Burnham 《Contributions to Mineralogy and Petrology》1984,85(1):58-66

Structural similarities between dry diopside melt and superhydrous albite melt (X _w >0.5) — both lack three-dimensional silicate units — suggest that thermodynamic relations may be similar. A model based on that assumption successfully predicts diopside melting relations and H₂O solubilities. For the model, the three partial differential equations describing solution of H₂O in albite melt for X _w >0.5 have been integrated for diopside melt from X _w =0 to X _w at least as large as 0.76, with two exceptions: an alternative partial differential equation for Henrian solution of H₂O in dilute melts was applied for X _w <0.20, and an alternative differential equation for the pressure dependence of a _w at pressures below 2 kbar was developed. The latter alternative equation yields relatively small ¯V_w's at low pressures rather than the large ¯V_w's calculated from the equation from the albite system. Available experimental solubility data are not precise enough to offer a choice between the small-¯V_w and large-¯V_w equations. Integration of all the partial differential equations was constrained solely by the P and T of a single experimentally-determined point on the H₂O-saturated solidus.Solubilities calculated by a Henrian-analogue solution model (a _di=X _di ² ) from the experimental H₂O saturated solidus lie outside experimental solubility constraints for dilute melts. On the other hand, a Henrian model (a _di=X_di) successfully predicts solubilities in dilute melts. The formulation of the Henrian model and magnitudes of model molar entropies of solution are consistent with the hypothesis that H₂O dissolves in diopside melt as an essentially undissociated species with little ordering on melt structural sites. That species could in turn be consistently, if not uniquely, interpreted to be molecular H₂O or a hydroxylation (OH^–) complex formed from nonbridging oxygens.  相似文献   

Glacial chronology of the Ruby Mountains-East Humboldt Range, Nevada     

William J. Wayne 《Quaternary Research》1984,21(3):286-303

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Fouling community of the Loxahatchee River estuary,Florida, 1980–81     

Benjamin F. McPherson  Wayne H. Sonntag  Maryann Sabanskas 《Estuaries and Coasts》1984,7(2):149-157

Monthly growth of the fouling community at eight test panel sites in the Loxahatchee River Estuary was related to salinity and temperature. Growth was lowest in January 1981 (averaging 23 g per m², dry weight), and increased during spring and early summer with increasing water temperature. Maximum growth occurred during early or midsummer at upstream locations, before river or canal discharge substantially reduced salinity, and in late summer at downstream locations. Growth was greatest at salinities slightly less than that of seawater and decreased at salinities less than about 10‰. Growth was suppressed throughout the estuary in August 1981, probably because of the sudden decrease in temperature and salinity, and perhaps the increase in physical scouring, caused by runoff from Tropical Storm Dennis. Large loads of nutrients transported to the estuary from storm runoff, however, may have subsequently stimulated growth, which increased in September 1981 to the maximum for the year (averaging 683 g per m², dry weight).  相似文献   

Lithophile elements in Huronian low-Ti continental tholeiites from Canada, and evolution of the Precambrian mantle     

Wayne T. Jolly 《Earth and Planetary Science Letters》1987,85(4)

Huronian basalts from central Ontario, Canada, dated at about 2450 Ma and associated with an early rifting episode, are classified as siliceous, low-TiNb tholeiites. They display strong enrichment in large-ion lithophile (LILE) and light rare earth (LREE) elements compared to modern oceanic lavas. The tectonic setting and geochemistry resemble Mesozoic rift-related low-Ti flood basalts, including the Ferrar Group of Antarctica, and the Parana and equivalent Etendeka volcanics of south Brazil and Namibia, respectively. High LILE/LREE ratios are also similar to subduction-related island arc tholeiites, and it is suggested that enrichment of the Huronian lithospheric mantle source occurred through ancient subduction of crustal material, probably during formation and consolidation of the Archean continental crust.Melting models suggest that Huronian subcontinental mantle source compositions, derived from least contaminated, aphyric, mafic end-members, had already undergone a complex evolution, including withdrawal of Archean basalts and hydrous enrichment in incompatible components. Despite several subsequent melting episodes and a second, probably magmatic, enrichment event, however, many aspects of the Huronian source signature were preserved, and appeared in later basaltic products of this mantle mass. Keweenawan volcanics, for example, dated at about 1100 Ma, preserve low P, Zr, Ti and HREE abundances.  相似文献   

Effects of mesoscale atmospheric convection cells on the waters of the East China Sea     

Clifford L. Trump  Stephen J. Neshyba  Wayne V. Burt 《Boundary-Layer Meteorology》1982,24(1):15-34

Currents and atmospheric parameters were measured in the East China Sea in February, 1975, as part of the AMTEX'75 program. These data were used to describe outbreaks of cold continental air over this warm and shallow sea. Particular emphasis was placed on describing the structure of mesoscale atmospheric cells embedded in the outbreaks and the effects of these cells on the water column.Two cold air outbreaks were recorded. Heat fluxes (latent plus sensible) as high as 1270 cal/cm² day were calculated. Evidence of mesoscale atmospheric cells was found during outbreaks in satellite imagery and in solarimeter data. The development of mesoscale cells was described by correlating fluctuations in the air temperature and absolute humidity records. The cells were found to be best developed when satellite imagery showed that they were of the closed variety. The data suggest that cellular activity matures from open to closed cell types.During the period of greatest development, a representative closed cell was 24–30 km in diameter, moved at 8 m s^-1 over the spar buoy, had a temperature fluctuation of 0.4 °C, an absolute humidity fluctuation of 0.4 g kg^-1, and wind speed and heat flux fluctuations of - 12.5%.A non-dimensional index, formed from the fluctuations of the air temperature and absolute humidity records, was used to indicate the passage of mesoscale atmospheric cells over the measuring site. Using this index as input and the fluctuations in the oceanic parameters at a depth of 20 m as output, it was found that the passage of mature cells was significantly correlated with temperature fluctuations and current fluctuations aligned 25 ° to the right of the wind about 45 min later.  相似文献   

ORGANIC COMPOUNDS AND GROUND-WATER POLLUTION     

Wayne A. Pettyjohn  Arthur W. Hounslow 《Ground Water Monitoring & Remediation》1983,3(4):41-47

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