The effect of extracellular polymers (EPS) on the proton adsorption characteristics of the thermophile Bacillus licheniformis S-86     

Janette Tourney  Bryne T. Ngwenya  J.W. Fred Mosselmans  Laurence Tetley  Gregory L. Cowie 《Chemical Geology》2008,247(1-2):1-15

This study investigated proton adsorption to an extracellular polymeric substance (EPS) producing bacterial strain, Bacillus licheniformis S-86, in order to characterise and quantify the contribution made by EPS to cell surface reactivity. Potentiometric titrations were conducted using both untreated cells and cells from which the EPS layer had been extracted. Surface-complexation modelling indicated the presence of four different functional groups in both untreated and EPS-free cells. These sites are assigned to phosphodiester, (pK_a 3.3–3.4), carboxylic (pK_a 5.3–5.4), phosphoryl/ (pK_a 7.4–7.5) and hydroxyl/amine (pK_a 9.9–10.1) type groups. The pK_a values for the four groups were very similar for untreated and EPS-free cells, indicating no qualitative difference in composition, but site concentrations in the untreated cells were statistically found to be significantly higher than those in the EPS-free cells for the pK_a 3.3–3.4 and pK_a 9.9–10.1 sites. Infrared analysis provided supporting evidence that site 2 is carboxylic in nature but did not reveal any difference in IR absorption between the native and EPS-free cells. Dissolved organic carbon (DOC) analysis conducted during this study indicated that DOC release by cells is significant, and that the EPS layer is the major contributor.  相似文献   

摄食水平对中华鳖(Trionyx sinensis)幼鳖生长和能量收支的影响     

雷思佳  叶世洲  胡先勤 《海洋与湖沼》2005,36(1):43-50

于2001年10月—2002年3月在深圳职业技术学院海洋生物技术实验室进行摄食-生长实验(实验周期为56天)。实验在水温30℃的条件下进行,设饥饿、1%、2%和饱食4个摄食水平,研究了中华鳖(Trionyxsinensis)幼鳖(296.60—396.09g)的生长和能量收支。结果表明,中华鳖幼鳖的特定生长率随摄食水平的增加,其湿重、干物质、蛋白质和能量的特定生长率均呈二次曲线增加,其中干物质的特定生长率(SGRdr)与摄食率(Rl)的关系式为SGRdr=-0.3621+0.8809Rl-0.1352Rl2(r2=0.896,n=26,P<0.01);除湿重的转化效率外,干物质、蛋白质和能量的转化效率在2%组均达到最大,分别为27.47%、31.48%和25.01%;摄食水平对中华鳖氨氮排泄率和尿素氮排泄率以及氨氮占总氮排泄率的比例均有显著影响(P<0.01),总氮排泄率、氨态氮排泄率和尿素氮排泄率均随摄食水平的增加而升高,从饥饿组到饱食组的变动范围分别是4.71%—38.70%、3.50%—24.64%和1.21%—6.48%,而氨氮占总氮比例的变化规律与上述指的标略有不同,饥饿组的比例略高于1%组,摄食组的比例随摄食水平的增加而增加,该比例的变动范围是71.92%—83.20%,回归分析表明,幼鳖的总氮排泄率[μmol/(g·d)](GN)、氨氮排泄率[μmol/(g·d)](NH3N)及尿素氮排泄率[μmol/(g·d)](UN)与其蛋自质摄入率(%体重  相似文献   

围隔实验中水柱颗粒物色素的动态研究     

刘红 《海洋湖沼通报》2006,(2):75-81

用高效液相色谱法(HPLC)研究了围隔实验过程中水柱颗粒物主要色素的组成及动态变化,并就滤食性鱼类鲢对水体主要色素代谢的影响进行了讨论。结果表明:水柱中的主要色素有叶绿素a、异黄素、岩藻黄素、黄体素、玉米黄素和蓝藻叶黄素等,未放鱼的围隔中实验后期各种色素含量均降低到最低水平,有鱼的围隔中鱼类密度越高则色素含量降低越多。未放鱼的围隔中叶绿素a代谢产物———脱植基叶绿素a、脱镁叶绿素a、脱镁叶绿酸a与叶绿素a的比值均大于其它围隔,有鱼的围隔中鱼类密度大的这几个比值也比较大。  相似文献   

GIS新引擎——“真图”数据解决方案     

李德仁  ;郭晟  ;胡庆武  ;刘守军  ;魏建魁 《测绘软科学研究》2008,(3)

提出了一种基于移动测量系统技术的"真图"数据解决方案。一方面极大地丰富了GIS数据源,另一方面通过相应的真图影像数据管理软件及开发包扩展GIS功能,为各行业应用提供了地理信息数据解决方案。  相似文献   

基于MapInfo的住宅小区物业管理信息系统研究   总被引：1，自引：0，他引：1  

张萍  陈清国  赵文光 《地理空间信息》2008,6(6):19-22

对GIS在住宅小区物业管理中的应用进行了研究,并以武汉南湖花园为实例,以Mapinfo为开发平台,以VB6．0为开发工具,建立了南湖花园物业管理信息系统,该系统基本实现了物业管理的各种基本功能。  相似文献   

梁子湖水体P的季节变化与沉积物P释放初步研究   总被引：4，自引：0，他引：4  

熊汉锋  陈治平  黄世宽  谭启玲  丰民义  王运华 《湿地科学》2006,4(3):174-178

采用分级测定的方法对梁子湖沉积物中无机磷酸盐进行了分析,测定了梁子湖水体P的季节变化,并以室内模拟的方法研究梁子湖沉积物在pH值和温度控制下P的释放特征。研究表明,梁子湖水体P的含量呈明显的季节变化,冬季高,夏季低。沉积物无机磷(P i)中以钙磷(Ca—P)为主(55%~61%),铁磷(Fe—P)次之(28%~33%),铝磷(A l—P)最少(3%~5%)。在梁子湖的入水口和出水口,由于沉积环境影响到P的形态,P的释放明显较湖心高。在试验初期,由于扰动的影响,使得P的释放在第1天比第2天和第3天高,其后P的释放量则迅速增加。温度对P释放影响明显,其具体表现为,30℃时P释放达到峰值的时间比4℃时提前4 d,而且前者峰值比后者高出9倍。pH值对沉积物P释放同样有明显的影响,与正常状态下的湖水条件(pH值为8.5)相比,偏酸(pH值为5.5)和偏碱(pH值为11.5)条件下,P的释放量增加。  相似文献   

Simulating the effect of Nitrogen application on Wheat Yield by linking remotely sensed measurements with wtgrows simulation model     

V. K. Sehgal  C. V. S. Sastri 《Journal of the Indian Society of Remote Sensing》2005,33(2):297-305

A field experiment was conducted on wheat at New Delhi with five treatments of Nitrogen (N) fertilizer application (0, 30, 60, 90 and 120 kgha^-1). Relationship has been established between observed leaf area index (LAI) and remotely sensed vegetation indices. These relationships are inverted and used for predicting LAI from vegetation indices on different days after sowing. The “re-initialization” strategy is implemented in model WTGROWS in which initial conditions of model are changed so that the model simulated LAI match remote sensing predicted LAI. The model performance with re-initialization has been evaluated by comparing the simulated grain yield and total above-ground dry matter (TDM) values with the actual observations. The results show that in-season re-initialization is effective in model course correction by improving the simulated results of yield and TDM for different N treatments even though the model was run with no N stress condition. Model re-initialization at different days shows that the closer is the day of re-initialization to crop anthesis the more effective is model course correction. Also, the treatment showing maximum error in yield simulation without re-initialization shows maximum reduction in error by re-initialization. The approach shows that the remote sensing inputs can substitute for some of the inputs or errors in inputs required by crop models for yield prediction.  相似文献   

A multi-scale study on land use and land cover quality change: The case of the Yellow River Delta in China   总被引：5，自引：0，他引：5  

Xuegong Xu  Honghai Guo  Xiaoling Chen  Huiping Lin  Qiaoling Du 《GeoJournal》2002,56(3):177-183

This paper presents a case study of the Yellow River Delta in China, to trace land use and land cover changes during the past 20 years, with an emphasis on land quality changes. Three sets of data are used in this case study: remote sensing data derived from satellite images; crop yield data from statistics; and soil data collected by the researchers in the field. Our study reveals that at the regional scale, LUCC has taken place in a positive direction: vegetation cover has been expanding and crop yields per hectare have been on rise. However, while the overall eco-environment has improved, the improvement is uneven across the Delta region. At local levels, some areas show signs of increased salinization and declining organic content. Both natural forces and human activities are responsible for the LUCC, but human activities play a more important role. While some impacts of human activities are positive, the damages are often long-lasting and irreversible. We also conclude that it is necessary to use both macro data (such as remote sensing data) and micro data (data collected in the field) to study land quality change. The former are efficient in examining land quality changes at the regional scale, the latter can serve to verify ground patterns revealed from macro data and help to identify local variations, so as to get a comprehensive understanding of LUCC and promote sustainable land use and land management. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

富水水库中微生物成因的显微针铁矿及其对微量元素的富集   总被引：3，自引：0，他引：3       下载免费PDF全文

车遥  胡宝林  夏林  孙振亚  王利忠 《地球学报》2003,24(2):161-166

通过对湖北省富水水库坝基渗漏水及其析出物的研究，探讨了现代沉积环境（水库）中显微铁矿物形成的机理及微生物在铁的生物矿化中的作用和意义。对析出物中微量元素的富集特征也进行了初步研究。用化学分析方法测试出坝基渗漏水中阳离子以Ca^2 、Mg^2 、K^ 、Na^ 为主，Fe^2 和Fe^3 含量极少，阴离子以HCO3^-为主。析出物的化学成分以铁的氧化物为主。采用X射线衍射、差热分析、红外光谱和穆斯堡尔谱，以及透射电镜和扫描电镜等测试手段，分析研究了析出物的物相和形貌特征。析出物的主要矿物相为针铁矿、石英、伊利石、蒙脱石和微量方解石，呈弱结晶状态。由析出物中显微针铁矿特殊的形貌特征及其特征的穆斯堡尔谱图推测出富水水库沉积环境中显微针铁矿是由铁细菌形成。ICP－MS测试测试结果表明，析出物中不仅富集了铁，还富集了Yb、Pb、Dy、Zn、Co、Be、Eu、Sm和Tb等微量元素。微量元素的富集是微生物与显微针铁矿共同作用的结果。  相似文献   

丽江地震前后重力场变化的有限矩形位错模型分析   总被引：1，自引：0，他引：1       下载免费PDF全文

燕乃玲  李辉  申重阳 《地震学报》2003,25(2):172-181

研究了用有限矩形位错模型计算地壳形变引起的地面重力场变化的方法．以丽江MＳ7.0地震为例，讨论了确定用于模型计算的断层面参数的原则，并给出了结果．计算和分析了不同类型位错引起的重力场变化图象特征，并与丽江地震前后观测到的重力场变化进行比较．结果表明，在发震断层有限范围内模型可解释同震重力场的变化，但模型对于更大空间范围上的重力场变化并不能给出很好地解释.   相似文献