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This paper presents a technique developed for the retrieval of the orientation of crop rows, over anthropic lands dedicated to agriculture in order to further improve estimate of crop production and soil erosion management. Five crop types are considered: wheat, barley, rapeseed, sunflower, corn and hemp. The study is part of the multi-sensor crop-monitoring experiment, conducted in 2010 throughout the agricultural season (MCM’10) over an area located in southwestern France, near Toulouse. The proposed methodology is based on the use of satellite images acquired by Formosat-2, at high spatial resolution in panchromatic and multispectral modes (with spatial resolution of 2 and 8 m, respectively). Orientations are derived and evaluated for each image and for each plot, using directional spatial filters (45° and 135°) and mathematical morphology algorithms. “Single-date” and “multi-temporal” approaches are considered. The single-date analyses confirm the good performances of the proposed method, but emphasize the limitation of the approach for estimating the crop row orientation over the whole landscape with only one date. The multi-date analyses allow (1) determining the most suitable agricultural period for the detection of the row orientations, and (2) extending the estimation to the entire footprint of the study area. For the winter crops (wheat, barley and rapeseed), best results are obtained with images acquired just after harvest, when surfaces are covered by stubbles or during the period of deep tillage (0.27 > R2 > 0.99 and 7.15° > RMSE > 43.02°). For the summer crops (sunflower, corn and hemp), results are strongly crop and date dependents (0 > R2 > 0.96, 10.22° > RMSE > 80°), with a well-marked impact of flowering, irrigation equipment and/or maximum crop development. Last, the extent of the method to the whole studied zone allows mapping 90% of the crop row orientations (more than 45,000 ha) with an error inferior to 40°, associated to a confidence index ranging from 1 to 5 for each agricultural plot.  相似文献   
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克隆凡纳滨对虾极端体重个体的组织差异表达基因P23基因并进行生物信息学分析, 为进一步研究该基因的功能以及凡纳滨对虾的分子选育等研究提供信息。以凡纳滨对虾雌虾极端体重个体腹部肌肉为实验材料, 利用抑制消减杂交(SSH)技术构建极大体重雌性个体和极小体重雌性个体腹部肌肉组织正反向消减cDNA文库:正库(以极大体重个体为试验组, 以极小体重个体为驱动组, L-S)和反库(以极小体重个体为试验组, 以极大体重个体为驱动组, S-L)消减cDNA文库, 并采用实时定量技术分析P23基因的组织表达规律, 利用生物信息学对其功能和结构进行预测。以β-actin为看家基因检测两个文库的消减效率分别为210和25, 实时定量技术分析结果表明P23基因在体重的调节中起上调作用。P23基因编码区序列全长495bp, 编码165个氨基酸, GenBank登录号:JF806619。生物信息学分析发现P23蛋白部分序列含有强疏水性, 无跨膜螺旋结构, 两种信号肽预测都显示P23含有信号肽。  相似文献   
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采用PCR-RFLP法对379尾凡纳滨对虾α-淀粉酶基因(AMY)多态性进行检测,并分析AMY基因多态性与生长性状的相关性.结果表明:引物AMY-5与AMY-6扩增片段均具多态性,引物AMY-5扩增产物有CC,CT和TT 3种基因型,AMY-6扩增产物有AA,AB和BB 3种基因型.AMY基因AMY-5引物位点TT基因...  相似文献   
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To quantify the response to selection,heritability and genetic correlations between weight and size of Litopenaeus vannamei,the body weight (BW),total length (TL),body length (BL),first abdominal segment depth (FASD),third abdominal segment depth (TASD),first abdominal segment width (FASW),and partial carapace length (PCL) of 5-month-old parents and of offspring were measured by calculating seven body measurings of offspring produced by a nested mating design.Seventeen half-sib families and 42 full-sib families ofL.vannamei were produced using artificial fertilization from 2-4 dams by each sire,and measured at around five months post-metamorphosis.The results show that heritabilities among various traits were high:0.515士0.030 for body weight and 0.394士0.030 for total length.After one generation of selection,the selection response was 10.70% for offspring growth.In the 5"’ month,the realized heritability for weight was 0.296 for the offspring generation.Genetic correlations between body weight and body size were highly variable.The results indicate that external morphological parameters can be applied during breeder selection for enhancing the growth without sacrificing animals for determining the body size and breed ability;and selective breeding can be improved significantly,simultaneously with increased production.  相似文献   
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Seven growth-related traits were measured to assess the selection response and genetic parameters of the growth of Pacific white shrimp, Litopenaeus vannamei, which had been domesticated in tanks for more than four generations. Phenotypic and genetic parameters were evaluated and fitted to an animal model. Realized response was measured from the difference between the mean growth rates of selected and control families. Realized heritability was determined from the ratio of the selection responses and selection differentials. The animal model heritability estimate over generations was 0.44±0.09 for body weight (BW), and ranged from 0.21±0.08 to 0.37±0.06 for size traits. Genetic correlations of phenotypic traits were more variable (0.51–0.97), although correlations among various traits were high (>0.83). Across generations, BW and size traits increased, while selection response and heritability gradually decreased. Selection responses were 12.28%-23.35% for harvest weight and 3.58%–13.53% for size traits. Heritability estimates ranged from 0.34±0.09 to 0.48±0.15 for harvest weight and 0.17±0.01–0.38±0.11 for size traits. All phenotypic and genetic parameters differed between various treatments. To conclude, the results demonstrated a potential for mass selection of growth traits in L. vannamei. A breeding scheme could use this information to integrate the effectiveness constituent traits into an index to achieve genetic progress.  相似文献   
7.
为了研究我国海南地区某凡纳滨对虾(Litopenaeus vannamei)繁育群体的种质资源多样性,选取14个比利时野生对虾的EST微卫星标记,以240个中国人工选育的凡纳滨对虾个体为研究对象,检测这些标记在该养殖群体遗传多样性评估中的可行性,结果表明,有7个引物可扩增出清晰条带,其中5个引物(CNM-MG 345,...  相似文献   
8.
Pacific whiteleg shrimp(Litopenaeus vannamei) is an economically relevant shrimp species in many Asian countries. The specific objective of the current research was to assess microsatellite markers in screening the fastgrowth of domesticated L. vannamei stocks to establish a founder population for breeding-selection plans. The postlarvae produced by the reproduction of second generation broodstock were cultured in the same conditions throughout a five months growing period. Ninety juvenile shrimp were selected from the slow-, medium- and the fast-growth groups, and ten microsatellite markers were used to investigate their genetic diversity, and to understand the improvement of a breeding-selection scheme. Ten polymorphic loci(markers)(M1–M10) were produced at ten loci in this sample, among them Primer M8 was the highest polymorphic locus and M7 was the lowest one. A specific locus was found in the fast-growth group using Primer M5. The longest genetic distance(0.481) was determined between the fast- and medium-growth groups and the shortest(0.098) was between the slow- and medium-growth groups; therefore, the largest genetic identity(0.946) was observed between the slowand medium-growth groups and the smallest(0.667) was observed between the medium- and fast-growth groups.The Unweighted Paired Group with Arithmetic Average(UPGMA) dendrogram based on Nei's genetic distances provided two different groups; the first consist of the slow- and medium-growth groups and the second the fastgrowth group. Selection response and realized heritability for growth were 11.55% and 31.26%, respectively.Therefore, this set of microsatellite markers would provide a useful tool in shrimp breeding schemes.  相似文献   
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This overview compares and contrasts trends in the magnitude of the downward Particulate Organic Carbon (POC) flux with observations on the vertical profiles of biogeochemical parameters in the NE subarctic Pacific. Samples were collected at Ocean Station Papa (OSP, 50°N, 145°W), between 18–22 May 1996, on pelagic stocks/rate processes, biogenic particle fluxes (drifting sediment traps, 100–1000 m), and vertical profiles of biogeochemical parameters from MULVFS (Multiple Unit Large Volume Filtration System) pumps (0–1000 m). Evidence from thorium disequilibria, along with observations on the relative partitioning of particles between the 1–53 μm and >53 μm classes in the 50 m mixed layer, indicate that there was little particle aggregation within the mixed layer, in contrast to the 50–100 m depth stratum where particle aggregation predominated. Vertical profiles of thorium/uranium also provided evidence of particle decomposition occuring at depths ca. 150 m; heterotrophic bacteria and mesozooplankton were likely responsible for most of this POC utilisation. A water column carbon balance indicated that the POC lost from sinking particles was the predominant source of carbon for bacteria, but was insufficient to meet their demands over the upper 1000 m. While, the vertical gradients of most parameters were greatest just below the mixed layer, there was evidence of sub-surface increases in microbial viability/growth rates at depths of 200–600 m. The C:N ratios of particles intercepted by free-drifting and deep-moored traps increased only slightly with depth, suggesting rapid sedimentation even though this region is dominated by small cells/grazers, and the upper water column is characterised by long particle residence times (>15 d), a fast turnover of POC (2 d) and a low but constant downward POC flux.  相似文献   
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