雨生红球藻β-胡萝卜素酮化酶基因的cDNA和基因组DNA克隆及序列分析     

滕长英  张立  秦松  曾呈奎 《海洋科学》2006,30(8):20-27

根据已知的β-胡萝卜素酮化酶(β-carotene ketolase,BKT)的cDNA序列设计引物,利用长距离PCR扩增获得了bkt基因的cDNA完整编码片段及基因组DNA片段,并对这些片断进行了序列测定。cDNA和基因组DNA比对结果表明该基因至少包括5个内含子,它们的剪切位点皆符合GU-AG规律。在比对结果中同时还发现一个19bp的短序列重复,该重复序列在bkt的cDNA和基因组DNA上都发生了多次重复,推测在BKT的表达调控中可能具有一定功能。另外,在序列比对过程中还发现本实验所获得的cDNA和基因组DNA序列与前人报道的cDNA序列之间都存在多处单碱基差异和19bp的短序列差异。  相似文献   

A New Pursuit Mode of Nonlinear Wave Surface     

孙大鹏李玉成  郭海滨 《中国海洋工程》2004,18(2):257-266

The numerical mode of nonlinear wave transformation based on both the Laplace equation for water field and the Bemoulli equation for water surface is a kind of time-domain boundary problem with initial conditions. And the basis for establishing the numerical mode of nonlinear wave in time domain is to trace the position of wave free surface and to calculale the instantaneous surface height and surface potential function. This paper firstly utilizes the ‘0-1‘ combined BEM to separate the boundary by means of discretization of Green‘ s integral equation based on the Laplace equation, then separates the free surface of wave with FEM and derives the FEM equation of wave surface that satisfies the nonlinear boundary conditions. By jointly solving the above BEM and FEM equations, the wave potential and surface height could be obtained with iteration in time domain. Thus a new kind of nonlinear numerical mode is established for calculating wave transformation. The wave test in the numerical wave tank shows that the numerical simulation with this mode is of high accuracy.  相似文献   

Spectral Analysis of Nonlinear Drag Forces     

马汝建  李桂喜赵东 《中国海洋工程》2005,19(2):325-332

The spectral properties of nonlinear drag forces of random waves on vertical circular cylinders are analyzed in this paper by means of nonlinear spectral analysis. The analysis provides basic parameters for estimation of the characteristic drag forces. Numerical computation is also performed for the investigation of the effects of nonlinearity of the drag forces.The results indicate that the wave drag forces calculated by linear wave theory are larger than those calculated by the third order Stokes wave theory for given waves. The difference between them increases with wave height. The wave drag forces calculated by use of hnear approximation are about 5% smaller than their actual values when measured in the peak values of spectral densities. This will result in a safety problem for the design of offshore structures. Therefore, the nonlinear effect of wave drag forces should be taken into comidemtion in design and application of important offshore structures.  相似文献   

Numerical Solution of Lock-Release Gravity Current with Viscous Self-Similar Regime     

张立柱李行伟  陈国谦 《中国海洋工程》2004,18(1):157-162

Lock-release gravity currents with a viscous self-similar regime are simulated by use of the renormalization group(RNG) k - ε model for Reynolds-stress closure. Besides the turbulent regime with initially a slumping phase of a conslant current front speed and later an inviseid self-similar phase of front speed decreasing as t^-1/3(where t is the time measured from release), the viseous self-similar regime is satisfactorily reproduced with front speed decreasing as t^-4/5,consistent with well known experimental observations.  相似文献   

带乘性噪声的一类非线性系统的滤波算法     

褚东升  张征 《中国海洋大学学报(自然科学版)》2006,36(4):569-572

针对带乘性噪声的一类非线性系统,给出了1种带单重渐消因子的强跟踪状态滤波算法。该算法将非线性系统线性化后,采用了线性最小方差估计方法来进行状态估计,通过运用正交原理和引入渐消因子,使得滤波效果具有强跟踪的优良性能。该算法扩展了卡尔曼滤波在带乘性噪声非线性系统状态估计中的应用范围。仿真结果表明了该算法的有效性。  相似文献   

潮汐响应分析及非线性输入函数的研究   总被引：1，自引：0，他引：1  

李红岩  黄祖珂  陈宗镛 《海洋与湖沼》1989,20(4):330-337

本文采用对不同潮族进行带通滤波而后进行响应分析的方法对潮汐进行分析。引用第一级预报水位构成的非线性输入函数分析非线性潮。给出了预报水位4至6级的组合公式。较好地分析了8,10两族。本文还以坎门和金山咀为例研究了各种输入函数的分析效果,并以预报方差和谱结构来考察输入函数的合理性。  相似文献   

均匀水底上二维随机波面的二阶谱   总被引：1，自引：1，他引：1       下载免费PDF全文

孙孚  丁平兴  余宙文 《海洋与湖沼》1993,24(1):1-6

本文用摄动方法导出了均匀水底上精确至二阶的二维随机波动解。依据此解并将二阶谱定义为三阶矩函数的Fourier变换,首次给出了精确至二阶的二维随机波面二阶谱的理论表达式并简略地考虑了它的各个组成部分的物理意义。  相似文献   

A New Approach to High-Order Boussinesq-Type Equations with Ambient Currents   总被引：6，自引：3，他引：6  

王亚玲张洪生  缪国平朱良生 《中国海洋工程》2005,19(1):49-60

A new approach to high-order Boussinesq-type equations with ambient currents is presented. The current velocity is assumed to be uniform over depth and of the same magnitude as the shallow water wave celerity. The wave velocity field is expressed in terms of the horizontal and vertical wave velocity components at an arbitrary water depth level. Linear operators are introduced to improve the accuracy of the kinematic condition at the sea bottom. The dynamic and kinematic conditions at the free surface are expressed in terms of wave velocity variables defined directly on the free surface. The new equations provide high accuracy of linear properties as well as nonlinear properties from shallow to deep water, and extend the applicable range of relative water depth in the case of opposing currents.  相似文献   

黄海风暴潮和天文潮非线性耦合作用的数值研究   总被引：1，自引：2，他引：1  

赵永良  张延廷  陈则实 《海洋学报》1992,14(3):37-46

本文以4个主要分潮之和为开边界输入条件,对黄海天文潮及8114、7708、7303号台风潮与天文潮耦合作用进行数值模拟,计算结果与实测值基本相符,又通过模拟的流场和水位的变化,分析了不同风暴条件下,天文潮与风暴潮及其流场耦合作用的时空变化规律,从水位场流场整体上研究其相互关系及其动力机制。讨论了几种主要动力因素在非线性耦合作用中的作用,取得了一些有益的结果。  相似文献   

The Mathematical and Parameterized Expressions for Wave Induced Excess Flow of Momentum     

朱首贤丁平兴  张文静 《中国海洋工程》2007,21(1):137-146

Wave induced excess flow of momentum(WIEFM)is the averaged flow of momentum over a wave period due to wave presence,which may also be called 3-D radiation stress.In this paper,the 3-D current equations with WIEFM are derived from the averaged Navier-Stokes equations over a wave period,in which the velocity is separated into the large-scale background velocity,the wave particle velocity and the turbulent fluctuation velocity.A concept of wave fluctuating layer(WFL)is put forward,which is the vertical column from the wave trough to wave ridge.The mathematical expressions of WIEFM in WFL and below WFL are given separately.The parameterized expressions of WIEFM are set up according to the linear wave theory.The integration of WIEFM in the vertical direction equals the traditional radiation stress(namely 2-D radiation stress)given by Longuet-Higgins and Stewart.  相似文献