Estradiol and estriol suppress CYP1A expression in rainbow trout primary hepatocytes     

Elskus AA 《Marine environmental research》2004,58(2-5):463-467

Hepatic levels of the pollutant inducible enzyme, CYP1A, are strongly suppressed in spawning female fish, a phenomenon attributed to high plasma levels of the female sex steroid hormone, estradiol. To evaluate the contribution of estrogen metabolites to estradiol-mediated CYP1A regulation, we treated primary hepatocytes isolated from juvenile rainbow trout (Oncorhynchus mykiss) with vehicle, 17beta-estradiol, or the estrogen metabolite, estriol, alone and in combination with each other and with the potent CYP1A inducer, benzo[a]pyrene (B[a]P). We found dose-dependent suppression of B[a]P-induced CYP1A activity by both steroids relative to controls. At 10(-7) M doses, estradiol and estriol suppressed B[a]P-induced CYP1A activity by 3- and 2-fold, respectively. Although not statistically significant, mean basal CYP1A activity levels were 15- and 13-fold lower in estradiol and estriol treated hepatocytes, respectively, relative to vehicle treated controls. Combining doses of estradiol and estriol failed to produce synergistic suppression of either basal or B[a]P-induced CYP1A activity relative to treatment with either steroid alone. The observed suppression is well below the often strong suppression observed in spawning female fish. We conclude that factors in addition to estradiol and estriol are likely involved in producing sexual dimorphism in CYP1A expression observed in spawning fish.  相似文献   

溴素的有机化学──Ⅴ.溴代脂肪酸—2,3,5,6—四溴—1,4—苯二酯的合成     

崔伟  孙明昆  钱佐国  刘升一 《中国海洋大学学报(自然科学版)》1996,(3)

以1，4—苯二酚和不同的短链脂肪酸为基本原料，经溴代、加成、形成酰卤和酰化等反应，合成了六种新的溴化脂肪酸—2，3，5，6—四溴—1，4—苯二酯。通过对产物的碳、氢、溴等元素的定量分析，以及红外吸收光谱和氢核磁共振谱的研究，验证了化合物的结构。  相似文献   

一种浅海锚泊测流浮标系统的实验     

李文湑  孙寿昌 《海洋科学集刊》1984,(23):43-50

早在五十年代,海洋科技工作者已开始利用浮标布设海洋调查仪器,进行海上观测的试验工作。目前被采用的浮标系统,大体有三种类型:(1)锚泊浮标系统,利用各类自含式海洋调査仪器进行测量,定期收回资料;(2)遥测浮标系统,利用无线电遥控和收发各种海洋观测数据;(3)漂移浮标系统,利用浮标本身的漂移,进行海流和其他要素的观测。本文主要介绍浅海锚泊浮标系统的设计和使用情况。 对锚泊浮标系统的研究和实验,许多国家已做了大量有成效的工作。目前关于浮标的设计和锚泊方法尚无統一意见,但是可以看出,大多趋向于采用水下浮标张缆锚泊。这种方法使得浮标系统比起遥测浮标系统来,较为轻小、经济、方便,适合于进行多站同步观测。国外在海洋调查中经常利用这种系统作较长期的海流观测。 我所在六十年代初就已开始了锚泊浮标系统的研究、实验,1964年进行了新的研究设计工作,并在“全国海洋仪器会战”期间,完成了整套系统的计算设计与海上实验,经过鉴定,建议作小批量生产。 近年来,国家海洋局第一海洋研究所、中国科学院南海海洋研究所利用该浮标系统作过多次海上试用,取得了海流长期观测资料,初步考验了该系统的性能。我们最近又对某些部件作了一定的改进,并进行了海上实验。但是,实验也表明:此方法不宜在渔场区和台风盛行季节使用,因易被渔网拖损和丢失。现综合报告如下。  相似文献   

Numerical Simulation of Residual Circulation due to Bottom Rouglmess Variability Under Tidal Flows in A Semi-Enclosed Bay     

槐文信  T.  Komatsu  曾小辉 《中国海洋工程》2005,19(4):601-612

Nowadays there are some chronic serious environmental problems, such as eutrophication, blue tide and so on, in a complicated coastal zone or a semi-enclosed bay, because the water exchanges between an inner bay and an outer sea is weak compared with the supply of contaminant. Under this situation, a method to improve the water quality by 3-dimensional small unsymmetrical structures has been proposed by Komatsu et al. In this paper, several numerical simulations of the tidal current and concentration for various arrangements of bottom roughness in a semi-enclosed model bay are carfled out with a depth-averaged 2-D numerical model. The model is solved by the hybrid finite analytic method with nonstaggered grid. And the SIMPLES algorithm with Rhie and Chow＇ s momentum interpolation technique is used for the simulation. The effect of Komatsu＇ s method for water purification is examined by numerical simulation. The result of numerical experiment indicates that it is possible to generate a new tidal residual current and to activate a tidal exchange by bottom roughness arrangement only.  相似文献   

Ecology of the Symbiotic Coral Cladocora caespitosa (L.) (Faviidae, Scleractinia) in the Bay of Piran (Adriatic Sea): II. Energy Budget     

Christian  Schiller 《Marine Ecology》1993,14(3):221-238

Abstract. The temperate, symbiotic coral Cladocora caespitosa occurs naturally with variable numbers of zooxanthellae. This allows us to study differences in the physiology of symbiotic and "non-symbiotic" polyps and to correlate them with zooxanthellae activities.
Zooxanthellae density in "dark" (normal-colored) polyps was 50 times higher than in "white" (bleached) polyps.
The chlorophyll a content per zooxanthella was 3 times, and Chi c was 13 times higher in white polyps than in dark ones. O₂-flux experiments were conducted with colonies in situ and with single polyps - dark and white - under laboratory conditions. Two approaches were used to evaluate the contribution of zooxanthellae translocation products to animal respiration. Both revealed that the animal tissues derive a higher benefit from the zooxanthellae during periods of low water temperature than during warm periods.  相似文献   

北部湾三维潮流场数值研究(英文)     

刘学刚  叶安乐 《中国海洋大学学报(自然科学版)》1988,(4)

本文对北部湾三维潮波进行了数值研究,并将所得结果与现有观测资料作了比较。通过比较发现两者相当一致。文中还绘制了M_2和K_1分潮的同潮图,并对其作了讨论。可以断定,北部湾的日潮优势是由日潮共振所引起的。M_2和K_1分潮流的水平分布表明,强流区位于它们各自的蜕化无潮点附近。还讨论了分潮流的铅直结构,可以看出这种结构十分接近实际分潮流场的特征。  相似文献   

北黄海沉积物——水界面反硝化速率及影响因素研究   总被引：4，自引：0，他引：4  

白洁  王晓东  李佳霖  宋亮  于江华  魏皓  张桂玲 《中国海洋大学学报(自然科学版)》2007,37(4):653-656

探讨近海氮的循环机制,采用乙炔抑制法和现场静态箱法对北黄海夏季局部海域的反硝化速率进行了研究,该海域反硝化速率在2.5~5.8μmol/m2.h之间,平均4.85μmol/m2.h。影响其反硝化速率的主要因素为溶解氧,其次是温度。北黄海的反硝化速率低于珠江口和长江口海域。  相似文献   

使用简易测试平台检测马哈克扭力仪的性能     

田树川  宋协法  邱天霞 《海洋湖沼通报》2002,(3):71-75

本文描述简易测试平台的原理与制作，对马哈克MAIHAK MDS－820型扭力仪检测模拟负载的准确性进行探讨。  相似文献   

Excess CO2 and pHexcess in the Intermediate Water Layer of the Northwestern Pacific     

Andrey Andreev  Makio Honda  Yuichiro Kumamoto  Masashi Kusakabe  Akihiko Murata 《Journal of Oceanography》2001,57(2):177-188

Excess CO₂ and pH_excess showing an increase in dissolved inorganic carbon and a decrease in pH from the beginning of the industrial epoch (middle of the 19th century) until the present time have been calculated in the intermediate water layer of the northwestern Pacific and the Okhotsk Sea. It is concluded that: (1) The Kuril Basin (Okhotsk Sea) and the Bussol' Strait areas are characterized by the greatest concentrations of excess CO₂ at isopycnal surfaces due to the processes of formation and transformation of intermediate water mass. (2) The largest difference in excess CO₂ concentration between the Okhotsk Sea and the western subarctic Pacific (about 8 µmol/kg) is found at the = 27.0. (3) The difference in excess CO₂ between the western subarctic Pacific and subtropical regions is significant only in the upper part of the intermediate water layer ( = 26.7–27.0). (4) About 10% of the excess CO₂ accumulation in the subtropical north Pacific is determined by water exchange with the subarctic Pacific and the Okhotsk Sea.  相似文献   

海藻硫酸多糖911对小鼠脾淋巴细胞增殖反应及对细胞产生IL—2作用的影响     

王文涛  张永祥  周金黄  管华诗 《中国海洋大学学报(自然科学版)》1992,(Z1)

本文以~3H—TdR掺入法观察911对小鼠脾淋巴细胞增殖反应的影响并用CTLL细胞检测了其对IL—2的作用。体外实验结果表明,911对小鼠脾淋巴细胞增殖反应有明显的增强作用,以0.5μg/ml的浓度效果最为明显,相对增殖指数RPI可达200％;体内实验则以5mg/kg体重ip,连续7d效果最好,相对增殖指数RPI可达176％;911用药组小鼠IL—2的产生量均高于对照组,以5mg/kg和10mg/kg效果最好。以上实验证明,这一多糖是一种有希望的新免疫调节药物。  相似文献