中国花鲈细胞色素b基因片段的序列研究(英文)     

高天翔  张秀梅  陈大刚  张美昭  任一平  张亚平 《中国海洋大学学报(自然科学版)》2001,31(2)

参考鳗鲡等鱼类线粒体 DNA序列进行了中国花鲈线粒体 DNA细胞色素 b基因片断的引物设计、PCR扩增及其序列测定。得到中国花鲈的碱基序列为 4 10 bp,其 A、T、G、C含量分别为 10 1bp(2 4 .6 3% )、112 bp(2 7.32 % )、72 bp(17.56 % )、12 5bp(30 .4 9% ) ,与鳗鲡等其他鱼类相同基因片断序列碱基含量相似。  相似文献   

西太平洋暖池区热带波动的天气效应     

常美桂  王衍明 《海洋与湖沼》1995,26(S1):40-46

为了探讨西太平洋暧地区热带波动的天气效应，利用1980年2－9月140°E日本静止卫星纬度时间剖面卫星云图，分析了5d和14d左右周期热带波动云的演变特征，井综合分析了14d周期的云系演变型式与流场的关系，为预报热带中期天气变化规律提供了依据；热带波动中30－60d大气低频振荡的云量变化最显著，北半球夏半年热带波动的天气压主要在10°N－0°，各半年在10°N－10°S，超过这个区域热带云量的港分布型式就有明显的变化。  相似文献   

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri     

YU Ziniu  WEI Xiaohu  KONG Xiaoyu  YU Shanshan 《海洋学报(英文版)》2007,26(1):93-100

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   

西沙礁序列的磁性地层学研究   总被引：3，自引：0，他引：3  

张明书  周墨清 《海洋地质与第四纪地质》1996,16(3):61-65

利用西沙群岛西琛一井３３０ｍ以上井段的１４９块岩心标本，用美国２－Ｇ型超导磁力仪进行了古地磁测定，获得了一个从更新世至中新世晚期包括布容，松山，高斯和吉尔伯特四个极性时及若干亚极性的磁性地层柱，为礁序列的划分对比，提供了新的依据。  相似文献   

基于AHP法和灰色模式识别理论的海底管道系统路由定量风险评估   总被引：3，自引：1，他引：3  

徐慧 肖楠郭振邦  王金英 《海洋工程》2005,23(4):105-110

根据海底管道路由潜在风险的特点及风险类型,提出了一种将层次分析法（AHP法）和灰色模式识别理论相结合的海底管道系统路由定量风险评估方法,该方法利用AHP确定风险评价指标体系,运用灰色模式识别理论,建立识别结果标准,并结合实际工程进行计算,计算结果表明该方法是可行的.  相似文献   

长牡蛎剥离精卵的存活时间及受精能力   总被引：4，自引：1，他引：4  

王昭萍  王如才  文静  郑晓东 《海洋科学》1997,(1):5-6

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时间序列分析的一种新方法     

潘增宪 《海洋信息》1993,(11):20-21

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近58a来影响和登陆浙江的热带气旋统计特征分析   总被引：3，自引：0，他引：3  

俞燎霓  雷媛  曹美兰  陈春晓 《台湾海峡》2007,26(2):213-219

本文利用1949-2006年热带气旋资料,对58a来影响和登陆浙江热带气旋的登陆位置、登陆时间的年际、月际和日变化、登陆后的移速及路径变化与降水中心分布的关系、登陆后维持和衰减情况等进行了统计分析.统计结果表明：影响和登陆浙江的热带气旋有较明显的年际、月际和日变化特征;登陆浙江的热带气旋陆上维持时间与登陆时的强度正相关,登陆后12h内热带气旋衰减较快;登陆后移向偏北分量大的或移速加快的热带气旋,主要降水区出现在路径右侧的可能性大.  相似文献   

Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences     

WANG Jun  ZHANG Wen  SU Yongquan  DING Shaoxiong 《海洋学报(英文版)》2004,23(4):709-716

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.  相似文献   

Main Controlling Factors on Hydrocarbon Accumulation and Distribution in Marine Sedimentary Sequences in South China     

蔡立国  周雁  白振瑞 《中国地质大学学报(英文版)》2008,19(6):645-655

Multiple source rock assemblages were deposited in the sedimentary provinces in South China in geologic history,and some of them were destructed by and some survived against multiple tectonic movements.Therefore,multiple sources,mixed sources,and uneven distribution of sources occurred in the marine sedimentary basins in South China during the late stage of hydrocarbon pooling.Epidiagenesis of the marine carbonate reservoirs and its modification to reservoir poroperm characteristics determined the formation and the scale of natural gas pools.The exploration practices show that the large to medium gas fields mainly occur in areas with high-quality reservoirs.Detailed study of the paleo-oil accumulations and typical oil and gas reservoirs reveals that the basins experienced multiphase superimposition and modification,leading to the distribution of the Paleozoic paleo-oil accumulations and bitumen in the peripheral areas.The phenomenon that oil and gas production concentrates in the Sichuan basin indicates that the overall sealing conditions of a basin determine the oil/gas potentials and the scale of oil and gas production.This is a critical factor controlling the accumulation and distribution of gas in the marine sequences in South China.The early oil and gas pools in the Yangtze platform left billions of bitumen in the peripheral areas due to the destruction of seals.Since the Himalayan,"late-generation and late-accumulation" gas pools represented by the gas pools in the Sichuan (四川) basin were formed in the marine sedimentary sequences in South China as a result of the change of the sealing conditions.Current gas discoveries appear to be "paleo-generation and paleo-accumulation" gas pools but actually are "late-generation and late-accumulation" gas pools.These patterns of hydrocarbon pooling clearly depict themselves in western Sichuan basin and Weiyuan (威远)gas field.It is revealed that the gas pools in the Sichuan basin were mainly formed as a result of hydrocarbon phase change (thermal cracking of oil to gas),miscible migration,and dynamic equilibration since the Himalayan.A large number of gas pools were formed in the Himalayan and the gas pools in the marine sequences are characterized by late pooling; this kind of gas fields/pools are controlled by:(1) effectiveness of modification and superimposition of the marine basins,(2) effectiveness of the source rocks,(3) effectiveness of the overall preservation conditions,and (4) effectiveness of plays.  相似文献