泥蚶(Tegillarca granosa)生长因子受体结合蛋白2(GRB2)基因的克隆与表达分析   总被引：3，自引：0，他引：3  

董迎辉  项翔  姚韩韩  包永波  孙长森  林志华 《海洋与湖沼》2013,44(4):937-943

通过已构建的泥蚶(Tegillarca granosa)转录组文库, 利用SMART RACE技术扩增得到泥蚶Tg-GRB2基因的全长cDNA序列, 并对其生物信息学、组织表达及发育阶段表达特征进行了分析。结果表明, 泥蚶Tg-GRB2 cDNA全长1283bp, 开放阅读框为708bp, 编码236个氨基酸; 蛋白结构预测显示, Tg-GRB2蛋白包含SH3-SH2-SH3三个功能域, SH2结构域由两端的α-螺旋和中间反向平行的β-折叠片构成, SH3结构域主要由β-折叠片组成, 具备典型的结构特征; 氨基酸序列比对发现, Tg-GRB2与脊椎动物的同源性为62.1%—63.8%, 而与玻璃海鞘和日本血吸虫同源性较低。qRT-PCR检测结果显示: Tg-GRB2 mRNA在泥蚶血液、斧足、鳃、外套膜、闭壳肌、内脏团6个组织中都有表达, 而在血液中的表达量极显著地高于其它组织; 在泥蚶的不同发育阶段中, Tg-GRB2 mRNA在 2—4细胞胚胎、原肠胚、担轮幼虫、D形幼虫中均有较高的表达量, 而在D形幼虫中表达量最高, 表明Tg-GRB2基因在泥蚶早期发育中发挥重要调节作用。  相似文献   

Growth performance of the clam, Meretrix meretrix, breedingselection populations cultured in different conditions     

WANG Chao  CHAI Xueliang  WANG Hongxi  TANG Baojun  LIU Baozhong 《海洋学报(英文版)》2013,32(10):82-87

A breeding-selection program for the clam,Meretrix meretrix,was conducted since 2004.Two of the selection populations were generated with the shell color pattern as an additional selection criterion and named as SP(purple stripes)population and SB(black dots)population.The third-generation SP and SB populations(08G3SP and 08G3SB,respectively)were cultured at two commercial clam farms and a nursery pond and their shell lengths were compared.08G3SB clams had significantly larger sizes than 08G3SP clams at commercial clam farms(p<0.05),yet 08G3SB individuals were significantly smaller than 08G3SP individuals at the nursery pond(p<0.05).Then,we examined the growth of the fourth-generation SP and SB populations(10G4SP and 10G4SB,respectively)at a commercial farm,and found that the shell lengths of the 10G4SB clams increased at a significantly higher growth rate than the 10G4SP clams(p<0.05)from May to September,when the water temperature was between 24.2–27.5 C,while 10G4SB lost the growth advantage in the other months.These results indicated that SP and SB populations responded differently to environmental factors,so it is beneficial for the clam farmers to select a suitable population according to the culture environment.Furthermore,a diallel mating of the SB and SP populations was designed to investigate whether their hybrid population would show heterosis.However,the heterosis was not shown in this study,which might result from the slight genetic divergence between SB and SP populations.  相似文献   

Age, growth, and reproductive season of bluefish (Pomatomus saltatrix) in the Marmara region, Turkey     

Ceyhan  Tevfik; Akyol  Okan; Ayaz  Adnan; Juanes  Francis 《ICES Journal of Marine Science》2007,64(3):531-536

  相似文献   

Habitat selection and quality for multiple cohorts of young-of-the-year bluefish (Pomatomus saltatrix): Comparisons between estuarine and ocean beaches in southern New Jersey   总被引：1，自引：0，他引：1  

David L. Taylor  Ryan S. Nichols  Kenneth W. Able 《Estuarine, Coastal and Shelf Science》2007,73(3-4):667-679

In this study, seasonal and annual variability in the use of estuarine and ocean beaches by young-of-the-year bluefish, Pomatomus saltatrix, was evaluated by indices of abundance in coastal areas of southern New Jersey (1998–2000). Biological and physical factors measured at specific sites were correlated with bluefish abundance to determine the mechanisms underlying habitat selection. In addition, integrative and discrete indicators of bluefish growth were used to examine spatio-temporal dynamics in habitat quality and its effect on habitat selection by multiple cohorts of bluefish. Intra-annual recruitment to coastal areas of southern New Jersey was episodic, and resulted from the ingress of spring-spawned bluefish (hatch-date April) to estuarine beaches in late May to early June, followed by the recruitment of summer-spawned fish (hatch-date early July) to ocean beaches from July to October. Bluefish utilized estuarine and ocean beaches in a facultative manner that was responsive to dynamics in prey composition and temperature conditions. The recruitment and residency of bluefish in the estuary (1998–1999) and ocean beaches (1998), for example, was coincidental with the presence of the Atlantic silverside Menidia menidia and bay anchovy Anchoa mitchilli, the principal prey species for bluefish occupying these respective habitat-types. Bluefish abundance in the estuary (2000) and ocean beaches (1999–2000) was also correlated with water temperature, with the greatest catches of juveniles coinciding with their optimal growth temperature (24 °C). Bluefish growth, estimated as the slope of age–length relationships and daily specific growth rates, equaled 1.27–2.63 mm fork length (FL) d⁻¹ and 3.8–8.7% body length increase d⁻¹, respectively. The growth of sagittal otoliths was also used as a proxy for changes in bluefish size during and shortly before their time of capture. Accordingly, otolith growth rates of summer-spawned bluefish were greater at ocean beaches relative to the estuary and were explained by the more suitable temperature conditions found at ocean beaches during the mid- to late summer. Notwithstanding the fast growth of oceanic summer-spawned bluefish, individuals spawned in the spring were still larger in absolute body size at the end of the summer growing season (240 and 50–200 mm FL for spring- and summer-spawned bluefish, respectively). The size discrepancy between spring- and summer-spawned bluefish at the onset of autumn migrations and during overwintering periods may account for the differential recruitment success of the respective cohorts.  相似文献   

Rapid growth of an Archean continent by arc magmatism     

Paul A. Mueller  Joseph L. Wooden  David W. Mogk  Darrell J. Henry  Donald R. Bowes 《Precambrian Research》2010

The voluminous Meso- to Neoarchean rocks exposed in the Beartooth Mountains of the northern Wyoming Province of western North America comprise the Long Lake Magmatic Complex (LLMC), a variably metamorphosed and deformed association of igneous and meta-igneous plutonic rocks with SiO₂ ranging from at least 52 to 78 wt%. Within this compositional range, rock types include lineated amphibolites to hornblende-bearing gneisses of intermediate composition and multiple generations of foliated to unfoliated granitoids. Emplacement ages range between approximately 2.79 and 2.83 Ga, based on U–Pb zircon geochronology (SHRIMP). Field relations, elemental compositions, and geochronology indicate that these rocks do not represent a single fractional crystallization sequence, but rather, the LLMC was constructed by injection of numerous, discrete magmas as sill-like bodies over an ∼40 Ma period. Although there is a continuum of compositions in the LLMC, trace element abundances can be used to distinguish distinct sources and petrogenetic processes that can be broadly extrapolated to at least 3 compositional groupings: (1) trondhjemitic to granitic intrusive rocks with SiO₂ >70 wt%, (2) variably metamorphosed granodioritic orthogneisses with SiO₂ between 63 and 70 wt%, and (3) amphibole-bearing mafic to intermediate gneisses with SiO₂ between 52 and 63 wt%. Despite the range of SiO₂ contents, maximum LREE abundances are similar across the compositional range and, consequently, exhibit a wide range of (La/Yb)_n ratios (∼20–130). All LLMC rocks share a relative depletion in HFSE abundances similar to modern convergent margin magmas. Initial Sr and Nd isotopic compositions across the compositional range are consistently offset from typical bulk silicate earth (BSE) values and preclude unaltered derivation from primitive or depleted mantle. Common Pb isotopic data define a single array that lies above model crustal growth curves and, along with the Nd and Sr data, suggest relatively uniform interaction with, or derivation from, older lithosphere. The combined isotopic and elemental data suggest the LLMC resulted from simultaneous, rapid, and voluminous production of diverse magmas that represent melting of isotopically similar, but compositionally distinct, crustal and mantle sources. Dynamically, Meso- to Neo-archean crustal growth in the northern Wyoming Province appears to require an environment similar to a modern ocean–continent convergent margin with a comparable rate of crustal production and diversity of magma series. The resultant crust and associated mantle lithosphere (keel) appear to have suffered little-to-no modification prior to Laramide (Cretaceous) uplift and exposure.  相似文献   

东天山星星峡缝合带早古生代强过铝质花岗岩的研究及其地质意义   总被引：1，自引：0，他引：1  

毛启贵  肖文交  韩春明  方同辉  孙敏  袁超 《地质科学》2010,45(1)

铅炉子过铝质花岗岩带发育于中天山岛弧带南缘的星星峡缝合带内,为一长条形的二云母花岗岩。岩体地球化学特征是高Si、Al和K,低Mg、Fe的组份,全碱含量中等,σ=1.74～1.9,K_2ONa_2O,A/CNK1.1,属于高钾钙碱性过铝质花岗岩类(SP)。岩体的CaO/Na_2O和Al_2O_3/TiO_2比值都相对较低,分别为(0.06～0.07)和(48.9～55.3);REE、Nb、Y含量和Rb/Sr、Rb/Ba比值相对较高;Rb、Th和LREE相对富集,Ba、Si、Eu、Ti、P和HREE相对亏损;ε_(HF)(t)=-2.3～+1.6表明岩体还具有较高的锆石同位素特征,T_(2DM)变化范围为1573～1329 Ma。这些地质学和地球化学研究表明铅炉子二云母花岗岩形成于后碰撞的伸展环境中,是在高温、中低压条件下发生重熔形成的,其源岩为古老的地壳物质和年轻的增生物质通过物理风化作用形成泥质岩。本文通过测年获得二云母花岗岩锆石LA-ICPMS年龄为444.5±2.2 Ma,表明铅炉子过铝质花岗岩形成于晚奥陶世,是中天山岛弧带和公婆泉岛弧带的碰撞时间的上限,它也是中天山岛弧带与公婆泉岛弧带碰撞造山作用的产物,是中亚增生造山带的增生方式之一。  相似文献   

海拉尔盆地基底晚古生代adakite的发现及其地质意义   总被引：5，自引：1，他引：4  

章凤奇  陈汉林  曹瑞成  蒙启安  朱德丰  王志国 《岩石学报》2010,26(2):633-641

海拉尔盆地的前中生代基底隶属中亚造山带东段的兴蒙造山带。 在盆地基底地层中发现了具有adakite成分特征的粗面安山岩、英安岩和闪长玢岩。 这些火成岩与晚古生代沉积地层交互或伴生, 共同构成晚中生代裂陷盆地的基底。 地球化学研究表明, 这些火成岩基本上属于高钾钙碱性和准铝质岩石系列, 具有高SiO₂和Al₂O₃含量, 高Sr、Sr/Y和La/Yb值, 富集轻稀土(LREE), 亏损重稀土(HREE)、Y和高场强元素(HFSE),Eu表现弱的负异常或轻微的正异常, 相容元素Mg、Cr和Ni含量低, 这些特征与增厚下地壳部分熔融成因的adakite非常相似, 而明显不同于典型的由俯冲洋壳熔融形成的adakite。 样品的(⁸⁷Sr/⁸⁶Sr)_i值基本一致,为0.7041, (¹⁴³Nd/¹⁴⁴Nd)_i值为0.51243~0.51247, ε_Nd (t)为正值(+3.7~+4.5), 显示其岩浆源区可能源于弱亏损地幔, 或亏损地幔受到地壳物质混染。 本文认为海拉尔地区晚古生代adakite型岩浆很可能是由当时新底侵的玄武质下地壳在角闪岩相向榴辉岩相过度或榴辉岩相条件下部分熔融形成。 这些adakite岩石的出现反映了兴蒙造山带晚古生代受到了古亚洲洋的俯冲消减引起的强烈的地幔玄武质岩浆底侵作用, 并由此导致地壳垂向上显著的增生加厚过程。  相似文献   

大兴安岭北部塔河花岗杂岩体的地球化学特征及成因   总被引：3，自引：3，他引：0  

张彦龙  赵旭晁  葛文春  张吉衡  高妍 《岩石学报》2010,26(12):3507-3520

塔河杂岩体位于塔源-喜桂图缝合带北侧的额尔古纳地块东部,是早古生代侵入的花岗杂岩体。该杂岩体的主要岩石类型为正长花岗岩、二长花岗岩,少量碱长花岗岩和花岗闪长岩,辉长岩以包体存在于花岗岩中。岩石成因类型为典型的Ⅰ型后造山侵入体。岩体在野外地质特征、矿物组合、显微结构、化学成分及锆石Hf同位素特征等方面都表现出岩浆混合成因。在早古生代额尔古纳地块与兴安地块拼合后的后造山伸展拉张背景下,地壳和地幔都发生部分熔融,直接起源于亏损地幔的玄武质岩浆侵入到下地壳熔融的花岗质岩浆房,经结晶分异作用,形成了塔河杂岩体不同的岩石类型。花岗岩的εHf(t)为-0.8~+5.6之间,Hf模式年龄在0.9~1.5Ga之间,反映塔河花岗岩的源岩应该是在中-新元古代时期由亏损地幔起源的新生地壳物质。结合额尔古纳地块早古生代和中生代花岗岩锆石Hf同位素资料,我们认为额尔古纳地块在中-新元古代时曾发生过一次重要的地壳增生事件。  相似文献   

凡纳滨对虾(Litopenaeus vannamei)不同品系生长与耐高盐性状配合力及杂种优势分析     

王崇懿  王伦  刘建勇  傅学丽 《海洋与湖沼》2022,53(1):161-167

凡纳滨对虾(Litopenaeus vannamei)是优质经济虾类,为培育出具有更多不同优良经济性状的优质亲本,亟需开展杂交育种工作。以凡纳滨对虾5个不同品系进行不完全双列杂交产生的F1代资料为分析对象,利用统计学和混合线性模型对生长及耐高盐性状进行配合力和杂种优势分析。结果显示,对于生长性状,5个品系中检测到的一般配合力(GCA)效应值较低,实验环境下生长性状主要受特殊配合力的影响,Z♂×Z♀、P♂×XH♀2个组合生长性状的非加性效应明显,特殊配合力(SCA)效应值分别为0.69和0.30;对于耐高盐性状,品系P、S的GCA效应值最高,分别为0.83和0.53,P♂×XH♀、S♂×S♀2个组合耐高盐性状SCA效应值最高,分别为2.67和3.37;生长和耐高盐性状配合力方差组分结果显示,3个性状的特殊配合力方差占表型方差比例均高于一般配合力;5个杂交组合中体质量和高盐耐受性状的平均杂种优势范围分别为–19.23%~18.07%和–10.50%~20.97%。P♂×XH♀组合在生长和耐高盐性状方面杂种优势明显,可以考虑加强该组合在选育及生产方面的应用。  相似文献   

不同鲜重秋茄胚轴形态及其对幼苗生长的影响     

刘双双  杨升  刘星  陈秋夏  王金旺  郭晋敏  王佳玉  王文卿  吴伟志  梁立成  张小伟 《热带海洋学报》2022,41(6):56-66

为了探讨不同鲜重秋茄胚轴的表型性状和营养成分含量差异, 及其对幼苗生长的影响, 确定浙江省秋茄胚轴质量标准, 提高造林质量, 本研究采集4.0~5.0g、5.0~6.0g、6.0~7.0g、7.0~8.0g、8.0~9.0g、9.0~10.0g共6组不同鲜重的秋茄胚轴, 测定其表型性状、营养成分含量和幼苗生长性状。结果表明, 胚轴长度、横径、顶径随着胚轴鲜重的增加而显著增加。不同鲜重等级胚轴间的氮(N)、磷(P)、钾(K)和有机碳百分含量差异不显著, 但随着秋茄胚轴鲜重增加, 淀粉百分含量显著减小。单根胚轴的营养成分总含量随胚轴鲜重的增加而显著增加。胚轴的C:N、N:P和C:P值在鲜重7.0~8.0g等级时均显著高于其他鲜重等级。幼苗生长高、基径、叶片数及生物量随胚轴鲜重的增加而显著增加, 但在5.0~6.0g、6.0~7.0g和7.0~8.0g等级间幼苗茎干重、叶干重、净生物量和总生物量差异不显著。胚轴表型性状与幼苗生长指标之间的相关性均呈显著水平(P<0.05), 除胚轴P总含量与生长高之间的相关性不显著外, N、K、Na、有机碳、淀粉总含量与幼苗生长指标之间均呈显著正相关关系(P<0.05)。主成分分析和综合评价结果表明鲜重7.0g以上的胚轴优于其他鲜重的胚轴。秋茄幼苗生长高和净生物量与胚轴鲜重的回归分析R²分别为0.978和0.951, P均小于0.01。胚轴鲜重大于5.22g时, 幼苗生长高急剧增加; 鲜重大于8.74g时, 幼苗净生物量增长加快。因此, 秋茄胚轴鲜重越大, 营养物质含量越高, 越有利于幼苗生长。在浙江省的秋茄林种植中, 应优先选择鲜重7.0g以上的胚轴。  相似文献