Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis     

H. Jonsson  K. Viken Sandnes  D. Schiedek  R. Schneider  B. E. Grsvik  A. Goksyr 《Marine environmental research》2004,58(2-5):655

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.  相似文献   

Modelling of periodic wave transformation in the inner surf zone     

P. Bonneton   《Ocean Engineering》2007,34(10):1459-1471

In this paper, we analyse the ability of the nonlinear shallow-water (NSW) equations to predict wave distortion and energy dissipation of periodic broken waves in the inner surf zone. This analysis is based on the weak-solution theory for conservative equations. We derive a new one-way model, which applies to the transformation of non-reflective periodic broken waves on gently sloping beaches. This model can be useful to develop breaking-wave parameterizations (in particular broken-wave celerity expression) in both time-averaged wave models and time-dependent Boussinesq-type models. We also derive a new wave set-up equation which provides a simple and explicit relation between wave set-up and energy dissipation. Finally, we compare numerical simulations of both, the NSW model and the simplified one-way model, with spilling wave breaking experiments and we find a good agreement.  相似文献   

海带育苗水系微生物的监测与控制     

丁美丽  蒋本禹  费修绠 《海洋科学》1990,15(1):29-34

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海洋生物-光学算法研究──南黄海叶绿素浓度的估算     

傅克忖  曾宪模  李宝华  曾晓起  荒川久幸 《海洋与湖沼》1997,28(3):238-244

根据１９９５年９月中日合作南黄海现场观测资料，对南黄海生物－光学算法进行研究。首先对南黄海现场多光谱反射比数据进行因子分析，解反射比协方差矩阵的特征问题；解出特征值和对应的特征向量，求出主因子，然后建立叶绿素α对主因子的多元线性回归方程，并对算法进行回报和预报检验。计算结果表明，该算法具有较高精度，同时也显示出天气条件对估算精度产生较大影响。  相似文献   

大鹏湾夜光藻种群的季节变化和分布特征     

黄长江  齐雨藻  杞桑  吕颂辉  江天久 《海洋与湖沼》1996,27(5):493-498

于１９９０年３月－１９９２年６月对中国南海大鹏湾盐田附近海域的夜光藻种群动态及时空分布进行了综合调查，结果表明，夜光藻种群的出现期间一般为每年的１－６月，最早是１９９１年的１２月，娄量高峰期为３－５月，种群密度的波动极为显著；调查期间共发生８次赤潮，且都发生在３月初至５月初；大鹏湾夜光藻赤潮的发生特点是，赤潮的发生和消失突然，持续时间短，一般不超过３ｄ。  相似文献   

海湾扇贝肝素样多糖分离方法的研究     

王长云  管华诗  林洪 《海洋与湖沼》1995,26(S1):66-69

于1992年1月-1993年2月，对从海湾扇贝中提取获得的糖胺聚糖（GAG）用乙醇和溴化十六烷基三甲铵（CTAB）分级分离，并对所得制品用琼脂糖凝胶电泳和高效液相色谱验证，以研究一种简便、快速的分离提纯方法。结果表明，扇贝GAG水溶液在乙醇量60％时分离获得的级分，其GAG－GTAB给合物再用2.0 mol/LNaCl溶液解离得到的主要级分，琼脂糖凝胶电泳和高效液相色谱均显示出单一区带，红外光谱类似干肝素的。证明这一提纯过程是一简便、快速的分离提纯方法。  相似文献   

风浪频谱中的特征量   总被引：4，自引：3，他引：4       下载免费PDF全文

侯一筠  王涛 《海洋与湖沼》1993,24(2):126-131

在三参量风浪频谱的基础上对谱参量进行深入地研究,给出了谱参量与风场要素、波场要素的关系,提供了依据风场要素、波场要素及实测波浪资料计算谱参量的方法。从而可以依据上述因素直接计算出三参量风浪频谱。此外还根据谱宽度的变化,描述了风浪频谱的成长方式,解释了传统的波陡、波龄关系中经验常数的不同选取所代表的物理背景。  相似文献   

天然水体中酚的降解动力学     

吴欣然  夏福兴  吴玲  陈邦林 《海洋科学》1992,16(1):33-37

本文报道了天然水体中低浓度酚的降解动力学;计算了它的降解速率常数、降解活化能等。降解半表期是t_(1/2)=1.5d~(-1)(10℃),t_(1/2)=O.74d~(-1)(30℃)。低浓度酚的降解动力学跟天然水体中酚的降解情况比较接近。本文的结果对环境评价、蓄水库工程设计等很有参考价值。  相似文献   

A summary of the geology and oil habitat of the Eastern Flank Hydrocarbon Province of South Oman     

Aley Al-Marjeby  David Nash 《Marine and Petroleum Geology》1986,3(4)

The Eastern Flank Hydrocarbon Province borders the southeastern edge of the South Oman Salt Basin in the southern part of Oman. An intensive exploration programme by PDO over the past seven years has led to the discovery of almost 2 × 10⁹ m³ of oil with current production of some 15000 m³/d from six fields.In stark contrast to other oil habitats of the Arabian Peninsula, the main play involves rock units and geological events ranging in age from Late Precambrian to Quaternary Times, while trap development and charging are closely related to syn-tectonic salt dissolution.The principal reservoirs and seals are poorly consolidated, Palaeozoic clastics which drape, due to salt dissolution, over residual ‘cores’ principally composed of either Lower Palaeozoic sandstones (Haima Group), or Late Proterozoic carbonates (Huqf Group), or some combination of these. Oils have been geochemically correlated with algal source rocks of the Huqf Eastern Flank. Structures are considered to have been ‘charged’ from Late Mesozoic times onwards in conjunction with progressive subsurface salt removal and the onset of maturation of local source rocks. The effectiveness of ‘charging’ along the retreating salt edge is indicated by the small percentage of dry wells.Major oil zones are found in both anticlinal and truncation traps which are commonly developed around sandstone (Haima Group) cores. Oils show a wide range of characteristics but are typically heavy, early expulsion crudes, high sulphur with little associated gas. Bacterial transformation is not uncommon.Producing reservoirs mainly occur at relatively shallow depths (600–1200 m). Primary recovery factors of around 7% reflect the high density and viscosity of the Eastern Flank crudes and have initiated interest in Enhanced Oil Recovery (EOR) techniques. Two such pilot projects, involving steam and polymer flooding, are due to commence in 1984 and, if successful, will substantially increase the reserve base of these fields.  相似文献   

海洋专用CTD单芯电缆传输技术研究     

李红志  邓云 《海洋技术学报》2005,24(3):30-35

对单芯电缆传输这种通讯技术的原理进行了阐述;分析提出了部分电路、部件的技术指标;作为一种数据传输的手段,对单芯电缆传输技术在海洋专用CTD中的应用进行了相关论述.  相似文献