利用RAPD和AFLP标记初步构建太平洋牡蛎的遗传连锁图谱   总被引：24，自引：0，他引：24       下载免费PDF全文

李莉  郭希明 《海洋与湖沼》2003,34(5):541-551

利用RAPD和AFLP标记 ,以一回交家系 [(Miyagi×Hiroshima)×Miyagi]为作图群体 ,构建了太平洋牡蛎的遗传连锁图谱。用经过筛选的 3 3个RAPD引物和 1 1个AFLP引物组合 ,对父母本和 80个子代个体进行了遗传分析。共得到母本分离标记 1 93个 ,其中 1 44个符合1∶1孟德尔分离规律。父本分离标记 1 5 6个 ,其中 1 1 1个符合 1∶1孟德尔分离规律。雌性框架图包括 99个遗传标记 ,定位在 1 2个连锁群中 ,覆盖 985 2cM ,标记间平均间隔 1 1 3cM。另外有 3个三联体 ,7个连锁对 ,图谱共覆盖 1 1 6 5 7cM。雄性框架图包括 72个遗传标记 ,分布在 8个连锁群 ,覆盖 81 1cM ,标记间平均间隔 1 2 7cM。另外有 4个三联体 ,3个连锁对 ,图谱共覆盖93 1 8cM。  相似文献   

Validation and Improvement of Satellite-Derived Surface Solar Radiation over the Northwestern Pacific Ocean     

Kawai  Yoshimi  Kawamura  Hiroshi 《Journal of Oceanography》2005,61(1):79-89

The purpose of this study is to validate and improve satellite-derived downward surface shortwave radiation (DSSR) over the northwestern Pacific Ocean using abundant in situ data. The DSSR derivation model used here assumes that the reduction of solar radiation by clouds is proportional to the product of satellite-measured albedo and a cloud attenuation coefficient. DSSR is calculated from Geostationary Meteorological Satellite-5/Visible Infrared Spin-Scan Radiometer data in 0.05° × 0.05° grids. The authors first compare the satellite DSSR derived with a cloud attenuation coefficient table determined in past research with in situ values. Although the hourly satellite DSSR agrees well with land in situ values in Japan, it has a bias of +13∼+34 W/m² over the ocean and the bias is especially large in the low latitudes. The authors then improve the coefficient table using the ocean in situ data. Usage of the new table successfully reduces the bias of the satellite DSSR over the ocean. The cloud attenuation coefficient for low-albedo cases over the ocean needs to be larger in the low latitudes than past research has indicated. Daily and hourly DSSR can be evaluated from the satellite data with RMS errors of 11–14% and 30–33%, respectively, over a wide region of the ocean by this model. It is also shown that the cloud attenuation coefficient over land needs to be smaller than over the ocean because the effect of the radiation reflected by the land surface cannot be ignored.  相似文献   

Responses of phytoplankton and heterotrophic bacteria in the northwest subarctic Pacific to in situ iron fertilization as estimated by HPLC pigment analysis and flow cytometry   总被引：3，自引：0，他引：3  

Koji Suzuki  Akira Hinuma  Hiroaki Saito  Hiroshi Kiyosawa  Hongbin Liu  Toshiro Saino  Atsushi Tsuda 《Progress in Oceanography》2005,64(2-4):167

To verify the hypothesis that the growth of phytoplankton in the Western Subarctic Gyre (WSG), which is located in the northwest subarctic Pacific, is suppressed by low iron (Fe) availability, an in situ Fe fertilization experiment was carried out in the summer of 2001. Changes over time in the abundance and community structure of phytoplankton were examined inside and outside an Fe patch using phytoplankton pigment markers analyzed by high-performance liquid chromatography (HPLC) and flow cytometry (FCM). In addition, the abundance of heterotrophic bacteria was also investigated by FCM. The chlorophyll a concentration was initially ca. 0.9 μg l⁻¹ in the surface mixed layer where diatoms and chlorophyll b-containing green algae (prasinophytes and chlorophytes) were predominant in the chlorophyll biomass. After the iron enrichment, the chlorophyll a concentration increased up to 9.1 μg l⁻¹ in the upper 10 m inside the Fe patch on Day 13. At the same time, the concentration of fucoxanthin (a diatom marker) increased 45-fold in the Fe patch, and diatoms accounted for a maximum 69% of the chlorophyll biomass. This result was consistent with a microscopic observation showing that the diatom Chaetoceros debilis had bloomed inside the Fe patch. However, chlorophyllide a concentrations also increased in the Fe patch with time, and reached a maximum of 2.2 μg l⁻¹ at 5 m depth on Day 13, suggesting that a marked abundance of senescent algal cells existed at the end of the experiment. The concentration of peridinin (a dinoflagellate marker) also reached a maximum 24-fold, and dinoflagellates had contributed significantly (>15%) to the chlorophyll biomass inside the Fe patch by the end of the experiment. Concentrations of 19′-hexanoyloxyfucoxanthin (a prymnesiophyte marker), 19′-butanoyloxyfucoxanthin (a pelagophyte marker), and alloxanthin (a cryptophyte marker) were only incremented a few-fold increment inside the Fe patch. On the contrary, chlorophyll b concentration reduced to almost half of the initial level in the upper 10 m water column inside the Fe patch at the end of the experiment. A decrease with time in the abundance of eukaryotic ultraphytoplankton (<ca. 5 μm in size), in which chlorophyll b-containing green algae were possibly included was also observed by FCM. Overall, our results indicate that Fe supply can dramatically alter the abundance and community structure of phytoplankton in the WSG. On the other hand, cell density of heterotrophic bacteria inside the Fe patch was maximum at only ca. 1.5-fold higher than that outside the Fe patch. This indicates that heterotrophic bacteria abundance was little respondent to the Fe enrichment.  相似文献   

Responses of carbon dioxide in western tropical Pacific to 1991~1993 ENSO event     

Ma Liming 《海洋学报(英文版)》1997,16(2):201-214

On the basis of the data of the partial pressure of CO₂ (P_CO2)and the concentration of the total dissolved CO₂(T_CO2) in surface water during the expeditions in Nov.-Dec. 1991, the world ocean circulation experiment (WOCE) and Oct. 1992-Mar. 1993, the tropical ocean-global atmosphere coupled ocean-atmosphere response experiment (TOGA COARE) in the western tropical Pacific and of the comparison with data from 1986 to 1990 TOGA expeditions and that from Japan Meteorological Agency, the response of CO₂ in surface water to ENSO event is proved. The CO₂ signals indicated that the air-sea system in the western and central tropical Pacific from 1991 to 1993, except for a short period in autumn of 1992, was in a strong state of ENSO.
The change of CO₂ in the floating stations near 2°S, 155°E from Nov. 1992 to Mar. 1993 reflected the change of currents, water mass and its thermal and salt content during the forming and developing of ENSO.  相似文献   

A thermal centroid or a mass centroid?     

Mingqiang Fang 《Journal of Oceanography》2006,62(5):759-766

This study reveals the physical backgrounds of the geometric centroid and the thermal centroid of the Western Pacific Warm Pool (WPWP) and points out their differences. The geometric centroid (actually a very close approximation to the mass centroid) anomaly of the surface WPWP correlates more closely with the Niño-3 region sea surface temperature anomaly (Niño-3 SSTA, an important indicator of El Niño/La Niña events) than the surface thermal centroid. Taking the WPWP depth (or heat storage) into account, the “real” mass or thermal centroid of the WPWP might correlate better with the El Niño/Southern Oscillation (ENSO) signals.  相似文献   

太平洋波高分布及变化规律研究   总被引：4，自引：0，他引：4  

韩树宗  朱大勇  郭佩芳 《中国海洋大学学报(自然科学版)》2003,33(6):825-832

使用 Topex/ Poseidon卫星高度计 1 992年 1 0月～ 1 998年 1 2月连续 75个月 ,2 30个重复周期的有效波高资料对南北太平洋的有效波高进行了统计 ,分析了太平洋有效波高的多年平均、多年各月平均和多年各季平均的空间分布特征和时间变化规律。结果表明 ,太平洋波高分布具有明显季节变化的规律 ,与太平洋的风速分布特征具有良好的对应关系  相似文献   

Spatial variability in growth and reproduction of the Pacific oyster Crassostrea gigas (Thunberg, 1793) along the west European coast     

Joana F.M.F. Cardoso  Denis Langlet  Jos F. Loff  Ana R. Martins  Johannes IJ. Witte  Paulo T. Santos  Henk W. van der Veer 《Journal of Sea Research》2007,57(4):303-315

The Pacific oyster Crassostrea gigas was introduced in Europe for commercial purposes in the mid 1960s. It was initially thought that low winter temperatures would restrain this species' reproduction and settlement; however, its present distribution in areas where no introduction has taken place suggests that natural invasion and expansion has occurred. Along the European coast, wild populations of Pacific oysters are already found from northern Germany to southern Portugal. Whether C. gigas will continue to further expand through northern waters will depend on its physiological performance. In this study, the performance of wild oyster populations has been studied in terms of growth and reproduction at three stations: La Rochelle (France; 46°N), Yerseke (Oosterschelde estuary, The Netherlands, 51°N), and Texel (Wadden Sea estuary, The Netherlands, 53°N). The French population had the lowest somatic-shell mass ratio and an increase in maximum shell length, somatic and gonadal mass was observed from France to the Netherlands. In addition, mean oocyte diameter decreased significantly from south to north. The combination of increasing gonadal mass and decreasing oocyte volume suggests an increasing reproductive output in terms of egg numbers from France to The Netherlands. Differences in temperature between locations will at least be partly responsible for the observed patterns; however, other environmental factors (such as food availability, predation pressure, sediment type and/or seston concentration) cannot be excluded. Since smaller eggs (oocytes) are thought to have a longer development time, the environmental conditions along the Dutch coast may result in increased larval dispersal and possibly in further population expansion.  相似文献   

The contribution of nano- and micro-planktonic assemblages in the surface layer (0-30 m) under different hydrographic conditions in the upwelling area off Concepción, central Chile     

V. Anabalón  R. Escribano  M. Angélica Varas 《Progress in Oceanography》2007,75(3):396-414

In the highly productive region off central Chile, the structure and temporal and spatial variability of planktonic assemblages, and the factors that determine changes in this structure are poorly understood. In the region, wind-driven upwelling, heating by solar radiation and freshwater inputs are highly seasonal processes, which, together with higher frequency events, can promote changes in the planktonic communities, especially in the upper layer. This study focuses on the structure of nano- through to micro-planktonic assemblages (2-200 μm) of unicellular organisms (protists) in surface waters (0-30 m) during different hydrographic conditions. Samples were taken from a fixed shelf station off Concepción (COPAS time series Station 18) on eight occasions between September 2003 and August 2004. The nano-plankton flagellate-dominated fraction was numerically important during the whole period. Maxima in flagellate abundance and biomass occurred during the upwelling period (November-April samplings) but these maxima appear to be unrelated to the degree of water column stratification. The micro-plankton diatom-dominated fraction was usually the largest component in terms of biomass during the study period and the diatoms made important numerical contributions during the upwelling period, with maxima in abundance and biomass when water column stability was lowest. The dominant genera and morphotypes in each functional group were found throughout the study period, with maxima in abundance and biomass co-occurring under similar environmental upwelling conditions. The mean macro-nutrient concentrations (nitrate and silicate) were relatively high in the top 30 m during both upwelling and non-upwelling periods, and did not explain the maxima in plankton or functional group replacements. The persistence of the dominant taxa in the planktonic assemblages suggests a high degree of flexibility, though probably not at the specific level, to withstand the highly variable environmental conditions in this upwelling area.  相似文献   

北太平洋风海流季节变化的数值模拟     

刘秦玉  王建国  徐启春 《中国海洋大学学报(自然科学版)》1992,(2)

用绝热表层风海流模式,以1949～1979年COADS资料,研究北太平洋表层风海流及相应水位场随季节变化的特点、某些特定年表层风海流的异常。模式采用二次动量守恒的有限差分方法,保证了计算稳定性。在数值积分中考虑了陆界、水界条件和近岸地形对风海流及水位场的影响。计算表明:模式反映了北太平洋主要流系的季节变化规律,能较敏感地反映上层海洋对大气动力作用的响应。给出了北太平洋主要流系的强弱与黑潮大弯曲的对应关系。  相似文献   

The Continuous Plankton Recorder: concepts and history, from Plankton Indicator to undulating recorders   总被引：1，自引：0，他引：1  

P. C. Reid  J. M. Colebrook  J. B. L. Matthews  J. Aiken  Continuous Plankton Recorder Team 《Progress in Oceanography》2003,58(2-4):117

Alister Hardy conceived the Continuous Plankton Recorder (CPR) survey in the 1920s as a means of mapping near-surface plankton in space and time, interpreting the changing fortunes of the fisheries and relating plankton changes to hydrometeorology and climatic change. The seed he planted has grown to become the most extensive long-term survey of marine organisms in the world and the breadth of his vision becomes ever more apparent. The survey has now run for over 70 years and its value increases with every passing decade. Operating from ‘ships of opportunity’ the machines used are robust, reliable and easy to handle. Wherever possible, all the sampling and analytical methods have not been changed to maintain the consistency of the time series. Computerisation and the development of new statistical approaches have increased our ability to handle the large quantities of information generated and enhance the sensitivity of the data analyses. This overview, based on almost 900 papers, recounts the various phases in the history of the survey. It starts with the Indicator Survey (1921–1934), the deployment of the first CPR on the Discovery Expedition (1924–1927) and the early CPR survey in the North Sea (1931–1939). The survey reopened in 1946 after the Second World War and expanded across the North Atlantic to North America from 1959. Taxonomic studies were initiated and an emphasis was placed on patterns of distribution, which were seen to reflect the varying oceanographic conditions. The years 1968–1976 saw further expansion with operations even in the American Great Lakes, publication of a Plankton Atlas and initial evidence for a downward trend in plankton biomass. At about this time electronic instrumentation was attached to CPRs to make additional measurements and work was started on the development of a new generation of undulating Continuous Plankton and Environmental Recorders (CPERs). In 1976 the survey moved to Plymouth. Scientific priorities in the UK changed in the subsequent decade and funding became more difficult to secure even though some of the CPR papers being published at the time are now regarded as classics in plankton ecology. In 1988 the UK Natural Environment Research Council (NERC) decided to close the survey. An international rescue operation led to the creation of the Sir Alister Hardy Foundation for Ocean Science (SAHFOS) in 1990, which has continued with consortium funding from a number of countries, and from 1999 again included NERC. The scientific rationale of the survey has gained credibility as concern over climate change and other anthropogenic effects has grown and as the key role that plankton plays as an indicator of large-scale environmental conditions becomes ever more apparent. Recently, the survey became an integral component of the Global Ocean Observation System (GOOS) and expanded into the North Pacific. It plays a complementary role in many large international and multidisciplinary projects and is providing inspiration, advice and support to daughter surveys elsewhere in the world. At the start of a new millennium, Hardy’s vision from the 1920s is a powerful driving force not just in international biological oceanography, but in global environmental science.  相似文献