Application of Atomic Force Microscopy to Observation of Marine Bacteria   总被引：3，自引：0，他引：3  

Tomohiko Nishino  Eiko Ikemoto  Kazuhiro Kogure 《Journal of Oceanography》2004,60(2):219-225

The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Swimming endurance of haddock (Melanogrammus aeglefinus L.) at prolonged and sustained swimming speeds, and its role in their capture by towed fishing gears     

Breen  Mike; Dyson  Jamie; O'Neill  Finbarr G.; Jones  Emma; Haigh  Michael 《ICES Journal of Marine Science》2004,61(7):1071-1079

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Contribution of artificial reefs to the diet of the white sea bream (Diplodus sargus)   总被引：1，自引：0，他引：1  

Leitao  Francisco; Santos  Miguel N.; Monteiro  Carlos C. 《ICES Journal of Marine Science》2007,64(3):473-478

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大亚湾水域两种拟菱形藻的形态学鉴定及毒素分析   总被引：2，自引：0，他引：2  

邢小丽  杨军霞  康燕玉  高亚辉  梁君荣  林旭吟 《台湾海峡》2007,26(4):576-582

对2005年分离自广东大亚湾的两种潜在产毒拟菱形藻进行了形态学观察和毒性分析.通过透射电子显微镜(TEM)观察确认藻种为细弱拟菱形藻(Pseudo-nitzschia cuspidata)和多纹拟菱形藻(P.multistriata).采用高效液相色谱法(HPLC)对这两种藻的常规培养样进行软骨藻酸毒素检测,结果均未检测到软骨藻酸.鉴于以上两种拟菱形藻存在着潜在产毒特性,不同培养条件的诱导实验尚需继续.  相似文献   

经验模式分解对恒电量瞬态响应信号的预处理     

赵犁丰  王昱  赵永韬 《中国海洋大学学报(自然科学版)》2006,36(3):501-504,400

应用经验模式分解（EMD）将恒电量瞬态响应信号分解为不同时间尺度的内在模函数（IMF）分量。去除其中的小时间尺度的干扰噪声分量。然后经过拉普拉斯变换获得恒电量频谱以研究电化学腐蚀过程。  相似文献   

陀螺经纬仪的读数方法比较研究   总被引：2，自引：0，他引：2  

万朋  李宗春  李广云  张冠宇  牟爱国 《海洋测绘》2005,25(5):75-78

介绍了陀螺定向测量中三种不同的数据采集方法，分析了各种方法的优缺点。作者认为传感器采集数据自动化程度高，精度高，特别是运用位置敏感探测器（PSD）采样数据量大，具有一定的抗干扰能力，但在运用传感器采样时必须注意克服非线性误差。  相似文献   

Are invasive species most successful in habitats of low native species richness across European brackish water seas?   总被引：5，自引：0，他引：5  

Marjo Paavola  Sergej Olenin  Erkki Leppkoski 《Estuarine, Coastal and Shelf Science》2005,64(4):738-750

European brackish water seas (Baltic Sea, Black Sea and Sea of Azov, Caspian Sea) are subject to intense invasion of non-indigenous species (NIS). In these seas, salinity is the most important range limiting factor and native species seem to reach a minimum species richness at intermediate salinities. This trend, revealed by Remane in 1934 and later on confirmed by many other scientists, was compared to the salinity range of already established NIS in the European brackish water seas. It turned out that most NIS are well adapted to the salinities holding lowest native species richness, already in their native area, and that NIS richness maximum in brackish water seas occurs in the salinity intervals of native species richness minimum. A predictable pattern in the salinity range of NIS can be used as a tool in initial risk assessment of future invasions in brackish water seas, especially when mapping highly potential donor and recipient areas. A product of empty niches, suitable environmental conditions, and availability of proper vectors might be the most effective predictor for the invasibility of brackish water areas.  相似文献   

Composition and distribution of meiobenthos in amursky bay (peter the great bay, the east sea)     

Olga N. Pavlyuk  Julia A. Trebukhova 《Ocean Science Journal》2005,40(3):10-16

Qualitative and quantitative composition of meiobenthos was studied in Amursky Bay (Peter the Great Bay, the East Sea). Ten taxonomic groups were found, where nematodes were dominant. Density of meiobenthos in ground sediments of the Bay were not uniform, and the average density was measured at 126.4±62.3 ind. m^-2. 56 species of nematodes were detected, and dominant species wereSabatieria palmaris, Paracanthonchus macrodon, Sphaerolaimus limosus, S. gracilis andOncholaimium ramosum. Five taxocenes of nematodes were allocated based on the results of cluster analysis and species domination according to density. Low diversity in species composition of nematodes was noted in the northwestern part of the Bay, which is a zone of desalination, and in the eastern part, which is exposed to household drains.  相似文献   

添加纳米TiO₂的氯醚树脂的XPS分析     

向斌  李焰  张胜涛  侯保荣 《海洋科学》2006,30(1):63-67

在紫外线老化前后,利用光电子能谱(XPS)研究了分别添加金红石型和锐钛型纳米TiO2的氯醚树脂。研究发现,氯醚树脂中氯、氧元素具有表面富积现象——趋肤效应,而钛和碳元素在内层聚集;添加颜料粒子越小,在表面含量越大;金红石型纳米二氧化钛紫外屏蔽性好,减缓树脂老化;而锐钛型纳米二氧化钛具有显著的光催化活性,加速树脂老化。因此,金红石型纳米二氧化钛将是一种有前途的紫外线吸收剂。  相似文献   

渤海区域地质信息管理系统数据库设计方案   总被引：1，自引：0，他引：1  

张勇  杨作升  刘展  乔淑卿 《海岸工程》2002,21(4):1-5

数据库是GIS的基础。以渤海区域地质信息管理系统数据库为例，阐述了基于GIS的海洋地学管理信息系统中数据库的组织方法，及所包括空间数据库与属性数据库数据分类、分层方案，命名及编码规则，以及二者之间的联接。  相似文献