鳗弧菌(Vibrio anguillarum)M3菌株生长条件及其对蛋白酶产量的影响   总被引：2，自引：5，他引：2  

陈师勇  张培军  莫照兰  邹玉霞  张振冬  徐永立 《海洋与湖沼》2004,35(2):159-166

采用体外测定细菌浓度、胞外产物(ECP)蛋白含量和蛋白酶活力的方法，进行了鳗弧菌M3菌株在2216E培养基中的培养条件研究。结果表明，该菌株用固体培养基培养至24h左右，可得到较高的菌体浓度、ECP蛋白含量和蛋白酶活力。采用响应面分析方法设计实验，用SAS统计软件分析数据，得到NaCl浓度、pH值和温度对菌体生长及蛋白酶产量影响的回归模型。在2216E培养基的基础上，添加不同氮源、碳源物质以及不同浓度蛋白胨进行生长研究。结果表明，胰大豆蛋白胨能促进菌体生长及ECP蛋白分泌；NH4Cl与酪蛋白水解物可抑制蛋白酶的产生；牙鲆肌肉匀浆对菌体、ECP蛋白产量和蛋白酶产生有不同程度的促进作用；培养基中蛋白胨浓度为4％时菌体量与ECP蛋白含量达最高值，在蛋白胨浓度为2％时蛋白酶分泌量已稳定；1％的葡萄糖、蔗糖、甘油均能显著地提高菌体及ECP蛋白产量，却抑制了蛋白酶的产生。  相似文献   

Seasonal and Spatial Variations of Iceberg Drift off Dronning Maud Land, Antarctica, Detected by Satellite Scatterometers   总被引：1，自引：0，他引：1  

Shigeru Aoki 《Journal of Oceanography》2003,59(5):629-635

Seasonal and spatial variations of iceberg drift were studied using continuous satellite scatterometer images off Dronning Maud Land, East Antarctica. Generally, iceberg drift speed showed a westward increase to the Greenwich Meridian. Seasonal variations of the drift speed were high in autumn—early winter and low in spring, and their magnitudes also increased westward. Seasonal variations of the drift speed were significantly correlated with variations of sea levels at Syowa and Mawson Stations, and hence qualitatively consistent with geostrophic current variations. Thus, the scatterometer data are demonstrated to be useful in monitoring iceberg trajectory and oceanic current variations. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Estimation of Contamination of ERS-2 and POSEIDON Satellite Radar Altimetry Close to the Coasts of Australia   总被引：4，自引：0，他引：4  

X. Deng  W. E. Featherstone  C. Hwang  P. A. M. Berry 《Marine Geodesy》2002,25(4):249-271

It is broadly acknowledged that the precision of satellite-altimeter-measured instantaneous sea surface heights (SSH) is lower in coastal regions than in open oceans, due partly to contamination of the radar return from the coastal sea-surface state and from land topography. This study investigates the behavior of ERS-2 and POSEIDON altimeter waveform data in coastal regions and estimates a boundary around Australia's coasts in which the altimeter range may be poorly estimated by on-satellite tracking software. Over one million 20 Hz ERS-2 (March to April 1999) and POSEIDON (January 1998 to January 1999) radar altimeter waveform data were used over an area extending 350 km offshore Australia. The DS759.2 (5'resolution) ocean depth model and the GSHHS (0.2 km resolution) shoreline model were used together to define the coastal regions. Using the 50% threshold retracking points as the estimates of expected tracking gate, we determined that the sea surface height is contaminated out to maximum distance of between about 8 km and 22 km from the Australian shoreline for ERS-2, depending partly on coastal topography. Using the standard deviation of the mean waveforms as an indication of the general variability of the altimeter returns in the Australian coastal region shows obvious coastal contamination out to about 4 km for both altimeters, and less obvious contamination out to about 8 km for POSEIDON and 10 km for ERS-2. Therefore, ERS-2 and POSEIDON satellite altimeter data should be treated with some caution for distances less than about 22 km from the Australian coast and probably ignored altogether for distances less than 4 km.  相似文献   

A new type of Shipboard Meteorological Satellite Receiving-processing System   总被引：1，自引：0，他引：1  

Xie Simei  Hao Chunjiang  Zou Bin  Jing Qiyi  Liao Xiaohong  Sheng Xiaowen  Zhang Canrong 《海洋学报(英文版)》2000,19(1):35-46

INTRODUCTIONTheSMSRPSisstalledontheshipnavigatingovertheseaandreceivessatellitecloudmapsatanytimetoprovidereliablereal-timedataofmeteorologyandocean.Itisanimportantequipmentforsafeguardofshipnavigation.Chinahasthousandsofoceanicships.Butalmostallships,withonlyveryfewexception,arenotstalledbySMSRPS.Thecausesareasfollows:1.Thetechniqueisverycomplicated.2.Thedevelopmentcostisveryhigh.3.Ifweintroduceforeignequipment,thecostistooexpensive.4.Foreignequipmentanditssoftwarearecompletelyclo…  相似文献   

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay     

ZHANG Zhendong  ZHANG Peijun  MO Zhaolan  WANG Chunling  YU Yang 《海洋学报(英文版)》2005,24(4):155-161

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.  相似文献   

关于“包装产品的计量标准型一次抽检方案”的确定     

陈峥  赵维谦  李晓川 《中国海洋大学学报(自然科学版)》1989,(4)

本文给出了“包装产品的计量标准型一次抽检方案”,并通过一个实例说明本方案比一般《计量标准型一次抽检方案》更为优越。  相似文献   

利用卫星遥感监测南极海冰的研究和进展     

郝春江  解思梅 《海洋预报》1996,13(4):9-14

本文是根据南极“八五”国家攻关课题中的“南极海冰监测和预报”的考核目标，实现为南极考察船在 冰区中航行提供精确和清晰的冰图和预报。  相似文献   

Comparison of TOPEX/POSEIDON Altimeter Derived Wind Speed and Wave Parameters with Ocean Buoy Data in the North Indian Ocean   总被引：1，自引：0，他引：1  

Jignesh Kshatriya  Abhijit Sarkar  Raj Kumar 《Marine Geodesy》2001,24(3):131-138

Results of comparison exercises carried out between the state-of-the-art TOPEX/POSEIDON altimeter-derived ocean surface wind speed and ocean wave parameters (significant wave height and wave period) and those measured by a set of ocean data buoys in the North Indian Ocean are presented in this article. Altimeter-derived significant wave height values exhibited rms deviation as small as ±0.3 m, and surface wind speed of ±1.6 m/s. These results are found consistent with those found for the Pacific Ocean. For estimation of ocean wave period, the spectral moments-based semiempirical approach, earlier applied on GEOSAT data, was extended to TOPEX/POSEIDON. For this purpose, distributions of first four years of TOPEX/POSEIDON altimeter data and climatology over the North Indian Ocean were analyzed and a new set of coefficients generated for estimation of wave period. It is shown that wave periods thus estimated from TOPEX/POSEIDON data (for the subsequent two years), when compared with independent data set of ocean data buoys deployed in the North Indian Ocean, exhibit improved accuracy (rms ~ ±1.4 nos) over those determined earlier with GEOSAT data.  相似文献   

北京市基础测绘技术规程的探讨   总被引：1，自引：1，他引：0  

刘红霞  晁春浩 《北京测绘》2008,(1):29-31

分析了规程与现行相关法律、法规和标准的关系,探讨了北京市基础测绘技术规程的内容,介绍了其创新点和应用情况。通过分析介绍,该地方标准统一了北京市基础测绘的技术标准,规范了基础测绘成果,有利于实现测绘信息资源的共享和有效利用。  相似文献   

Methodology and use of tensor invariants for satellite gravity gradiometry   总被引：2，自引：1，他引：1  

Oliver Baur  Nico Sneeuw  Erik W. Grafarend 《Journal of Geodesy》2008,82(4-5):279-293

Although its use is widespread in several other scientific disciplines, the theory of tensor invariants is only marginally adopted in gravity field modeling. We aim to close this gap by developing and applying the invariants approach for geopotential recovery. Gravitational tensor invariants are deduced from products of second-order derivatives of the gravitational potential. The benefit of the method presented arises from its independence of the gradiometer instrument’s orientation in space. Thus, we refrain from the classical methods for satellite gravity gradiometry analysis, i.e., in terms of individual gravity gradients, in favor of the alternative invariants approach. The invariants approach requires a tailored processing strategy. Firstly, the non-linear functionals with regard to the potential series expansion in spherical harmonics necessitates the linearization and iterative solution of the resulting least-squares problem. From the computational point of view, efficient linearization by means of perturbation theory has been adopted. It only requires the computation of reference gravity gradients. Secondly, the deduced pseudo-observations are composed of all the gravitational tensor elements, all of which require a comparable level of accuracy. Additionally, implementation of the invariants method for large data sets is a challenging task. We show the fundamentals of tensor invariants theory adapted to satellite gradiometry. With regard to the GOCE (Gravity field and steady-state Ocean Circulation Explorer) satellite gradiometry mission, we demonstrate that the iterative parameter estimation process converges within only two iterations. Additionally, for the GOCE configuration, we show the invariants approach to be insensitive to the synthesis of unobserved gravity gradients.  相似文献