TEM evidence for intracellular accumulation of lead by bacteria in subsurface environments     

Nicolas Perdrial  Nicole Liewig  Jean-Emmanuel Delphin  Franoise Elsass 《Chemical Geology》2008,253(3-4):196-204

We monitored near-surface atmospheric fallout (15-cm above ground) and soil solution (at 15, 35 and 55 cm below ground) derived nanoparticles over an 8-month period by collecting the particles directly onto TEM grids in anthropogenically-influenced (vineyard) and pristine (native forest) sites in France. Particle clusters trapped on the grid were selected randomly and individual particles were binned into eight different groups (euhedral clays, weathered clays, salts, oxi-hydroxides, bacteria, non-living organic matter, aggregates and undetermined). Bacteria represent 9–23% of the collected nanoparticle area (ave. 9.4% and 18% for two atmospheric collection sites and ave. 23% for soil infiltration samples). Bacteria were most often associated with non-living organic matter and comprised a variety of morpho-types. Interestingly, 45% of all the bacteria analyzed by transmission electron microscopy and electron dispersive spectroscopy (TEM-EDX) showed the presence of intracellular grains significantly enriched in lead and phosphorus. Intracellular sequestration of Pb into polyphosphate bodies has been observed in the laboratory, but this is the first observation of this phenomenon in a natural environment. Furthermore, this suggests that microbial-bound Pb may be an important transport mechanism in subsurface environments.  相似文献   

A geological and geotechnical investigation of the settlement area of Zümrüt Building (Konya, Turkey) which caused 92 fatalities due to its collapse     

Adnan Özdemir 《Environmental Geology》2008,53(8):1695-1710

This study examines the local geological conditions and soil structure as possible causes of the collapse of the Zümrüt Building 2 February 2004. This catastrophe resulted in 92 fatalities and 35 injuries. This study also examines other views which claim weak soil structure, elastic and consolidation settlement of soil and excessive groundwater extraction as well as subsidence resulting from the underground silt erosion as possible factors. Zümrüt Building was constructed on normally consolidated, low plasticity clay. The underground water table was 30 m in depth. The internal friction angle of soil was 8°–30°, its cohesion was between 34 and 127 kN/m² and standard penetration test numbers varied between 11 and 50. The underground water level beneath Zümrüt Building had risen 4.5 m since its construction. Therefore the claim that subsidence resulting from the decrease of underground water level contributed to the collapse is incorrect. Secondly the settlement, resulting from the filling up of the pores created by the silt receding with the underground water, was 4.4 mm in total, and attributing this as the primary cause of the collapse is also incorrect. Soil properties, in situ and laboratory test results showed that the existing and/or expected settlement and the differential ground settlement in the Zümrüt building vicinity had the potential to cause structural damage. The tensile stresses caused by differential settlements recorded here are thought to be an indicator, but not the main cause contributing to the collapse of the building. The Zümrüt Building collapse was due to several compounding mistakes during the construction phase. These were geotechnical and other project faults and the use of low quality construction materials. The resulting catastrophe caused 92 fatalities, 35 injuries and a material loss of approximately US$7 million.  相似文献   

Soil contamination from urban and industrial activity: example of the mining district of Linares (southern Spain)   总被引：1，自引：0，他引：1  

J. Martínez  J. F. Llamas  E. de Miguel  J. Rey  M. C. Hidalgo 《Environmental Geology》2008,54(4):669-677

The Linares region (southern Spain) has been subjected to two important sources of pollution: the intensive mining works and the urban-industrial activity. To obtain a geochemical characterisation of the soil, 31 trace elements were analysed and 669 soil samples were collected. By means of clustering analysis, we identified groups of elements and grid squares in which relations could be established concerning soil lithology, urban and industrial activities and the degree of pollution impact; in addition, we were able to characterise the geochemical background of the study area. The multivariate study led us to identify four factors. Particularly important was factor 2, which represented the elements associated with mineral paragenesis (Cu, Pb, As, Co, Mn, Zn, Sn, Ba). This factor also contains elements related with an urban-industrial activity, such as Pb, Cu, Zn, As and Ba. Furthermore, we identified factor 4, associating Ni, V and Cr, and which is related to the use of fuels.  相似文献   

Plant distribution at the mobile dune scale and its relevance to soil properties and topographic features     

Xiaoan Zuo  Halin Zhao  Xueyong Zhao  Yirui Guo  Yulin Li  Yayong Luo 《Environmental Geology》2008,54(5):1111-1120

A survey was conducted in an 11-year recovery mobile dune (RMD11) and a 20-year recovery mobile dune (RMD20), in Horqin Sandy Land, Northern China, to determine plant distribution at the mobile dune scale and its relevance to soil properties and topographic features. The results showed that (1) vegetation cover and species number increased from dune top to bottom in the restoration process of mobile dune; (2) the average value of soil organic C, total N, pH, relative height of sampling site, very fine sand content and soil water contents (40−60 and 60−80 cm) of RMD11 were less than that of RMD20, respectively, and there were significant differences (P < 0.05) between the two dunes; (3) soil resources were redistributed by shrub restoration and relative height of sampling site on dune. The distribution of sand pioneer plant, Agriophyllum squarrosum, was positively related to the relative height of sampling site and soil water content, while that of other herbaceous plants was positively related to soil nutrients in the restoration process of mobile dune. These results suggest that at mobile dune scale, plant distributions are determined by a combination of soil properties and topographic feature. Much effort should be made to preserve the interdune lowland and to improve the level of soil nutrients on mobile dune.  相似文献   

<Superscript>226</Superscript>Ra, <Superscript>232</Superscript>Th and <Superscript>40</Superscript>K activities in soils of Cuihua Mountain National Geological Park,China     

Xinwei Lu  Xiaolan Zhang 《Environmental Geology》2008,56(2):353-357

Gamma activity from the naturally occurring radionuclides namely, ²²⁶Ra, ²³²Th, the primordial radionuclide ⁴⁰K was measured in the soil of Cuihua Mountain National Geological Park, China using γ-ray spectrometry technique. The mean activity of ²²⁶Ra, ²³²Th and ⁴⁰K were found to be 27.2 ± 6.5, 43.9 ± 6.2 and 653.1 ± 127.6 Bq kg⁻¹, respectively. The concentrations of these radionuclides were compared with the typical world values and the average activities of Chinese soil. The radium equivalent activity, the air absorbed dose rate, the annual effective dose rate, and the external hazard index were evaluated and compared with the internationally approved values. All the soil samples have Ra_eq lower than the limit of 370 Bq kg⁻¹ and H _ex less than unity. The overall mean outdoor terrestrial gamma dose rate is 66.3 nGy h⁻¹ and the corresponding outdoor annual effective dose is 0.081 mSv.  相似文献   

Environmental risk evaluation of the use of mine spoils and treated sewage sludge in the ecological restoration of limestone quarries     

M. M. Jordán  S. Pina  F. García-Orenes  M. B. Almendro-Candel  E. García-Sánchez 《Environmental Geology》2008,55(2):453-462

The ecologic restoration criteria in areas degraded from extraction activities require making use of their mine spoils. These materials do not meet fertility conditions to guarantee restoration success and therefore, need the incorporation of organic amendments to obtain efficient substratum. Reducing the deficiencies in the organic material and restoration material nutrients with the contribution of treated sewage sludge is proposed in this work. This experiment was based on a controlled study using columns. The work was conducted with two mine spoils, both very rich in calcium carbonate. The first mineral, of poor quality, came from the formation of aggregates of crushed limestone (Z). The other residual material examined originated in limestone extraction, formed by the levels of interspersed non-limestone materials and the remains of stripped soils (D). Two treatments were undertaken (30,000 and 90,000 kg/ha of sewage sludge), in addition to a control treatment. The water contribution was carried out with a device that simulated either short-duration rain or a flooding irrigation system in order to cover the surface and then percolate through the soil. The collection of leached water took place 24 h after the applications. Different parameters of the leached water were determined, including pH, electrical conductivity, nitrate anions, ammonium, phosphates, sulphates and chlorides. The values obtained for each irrigation application are discussed, and the nitrate values obtained were very elevated.  相似文献   

Impact of lightning on organic matter-rich soils: influence of soil grain size and organic matter content on underground fires     

Vittorio Zanon  Fátima Viveiros  Catarina Silva  Ana Rita Hipólito  Teresa Ferreira 《Natural Hazards》2008,45(1):19-31

A detailed study was carried out on a piece of land that had been struck by lightning during the violent rainstorm that raged over the Island of S?o Miguel (Azores Archipelago) in late October 2006. Temperature and gas measurements (CO₂, CO, H₂S and CH₄) were performed in four study trenches, dug in an area of ∼3 m², where an underground fire had been initiated by the impact with a lightning stroke, followed by the emission of a column of gases and smoke. The soil under study was originally a well-pedogenized about 80 cm thick bed, made of volcanic clayey to silty tephra fallouts and contained 5.5–9.7% of organic matter. The underground fire was monitored for one week and revealed a peak release of 404 ppm CO and 3.4% CO₂ originating from a horizon located about 45 cm under the soil surface. Measurements of temperature, performed one week after the impact, indicated a maximum value of 326°C inside the soil, while 516.5°C were measured on the surface of a lava block interred about 20 cm under the surface. Subsequently, a stratigraphic and sedimentologic study proved the role of the grain-size of the soil and of the organic matter content of the different horizons of the impact area, in determining the ratio between anoxic/oxidised combustion conditions and in the progress of the process itself. It was also noticed that combustion was not total all over in the soil bed and that the process had slightly migrated toward SW during the observation period. The combustion process went on for about ten days, in spite of several other violent rainstorms, until it was artificially extinguished through the excavations made to obtain study trenches. This particular circumstance evidenced the potential natural hazard represented by this kind of atmospheric event, especially in a land where the volcanic nature of the soil may easily mislead inexperienced observers and, consequently, delay proper action.  相似文献   

The effect of naturally acidified irrigation water on agricultural volcanic soils. The case of Asembagus, Java, Indonesia     

A.M.D. van Rotterdam-Los  S.P. Vriend  M.J. van Bergen  P.F.M. van Gaans   《Journal of Geochemical Exploration》2008,96(1):53-68

Acid water from the Banyuputih river (pH  3.5) is used for the irrigation of agricultural land in the Asembagus coastal area (East Java, Indonesia), with harmful consequences for rice yields. The river water has an unusual composition which is caused by seepage from the acidic Kawah Ijen crater lake into the river. This unique irrigation setting allows the study of soil acidification in situ. This paper assesses the effects of volcanogenically contaminated irrigation water on the chemical properties of the agricultural soils.The changes in soil properties were evaluated by comparing samples taken from the topsoil and sub-soil (1–3 m depth) from areas irrigated with acid water and areas irrigated with neutral water. The field survey thus resulted in four soil categories. Bulk soil composition, organic matter content, moisture content and particle size distribution were determined. Reactive phases were quantified with the selective extractions 1 M KCl, 0.1 M Na-pyrophosphate and 0.2 M acid ammonium oxalate (AAO).By comparing the four soil categories it is shown that the use of the naturally polluted irrigation water has had a large influence on the chemical composition of the topsoil. The composition of the soil solution has changed over the entire investigated soil profile. Furthermore the acid irrigation water has strongly modified the composition of the reactive phases, extracted as KCl, pyrophosphate, and AAO extractable elements, and also the bulk soil composition has been significantly modified. Overall this has resulted in the net dissolution of some elements and the net precipitation of others. The changes in the reactive phases and bulk soil composition are only apparent in the topsoil (0–20 cm) but not in the deeper soil.  相似文献   

Effect of Inter-Column Spacing on Soil Stresses due to Vibro-Installed Stone Columns: Interesting Findings   总被引：1，自引：0，他引：1  

Hesham Elshazly  Mohamed Elkasabgy  Azza Elleboudy 《Geotechnical and Geological Engineering》2008,26(2):225-236

This paper reveals the interesting relation between the inter-column spacing and the corresponding alteration of soil state of stresses due to the vibro-installation technique. This relation is inferred from analyses for load settlement records of various field load tests, performed for stone columns arrangements with different inter-column spacing values. In order to have adequate confidence in the findings, a well-documented case history, involving three columns patterns along with their relevant field and laboratory test results, is utilized for this study. Moreover, a well-tested finite element model, capable of simulating both elasto-plastic and time dependent soil deformations as well as pore water pressure building and dissipation, is employed in the analysis. Instead of determining the soil response to the test load, based on known initial soil stresses and material properties, the analysis is inversely posed to determine the soil initial stresses, based on the recorded settlements and the post-installation material properties. The alteration in the soil state of stress is represented by the increase in the post-installation horizontal to vertical stress ratio, K*, as a function of the inter-column spacing. It is found that this alteration experiences a systematic decrease in its magnitude as the inter-column spacing increases.  相似文献   

潮湿环境下古代土遗址的原位保护加固研究   总被引：3，自引：1，他引：2  

周环  张秉坚  陈港泉  赵海英  曾余瑶  郭青林  李最雄  王旭东 《岩土力学》2008,29(4):954-962

古遗址的保护是文物保护工作中最复杂的问题之一,而土质文物是文物保护中最难保护的文物,所以古代土遗址的保护已在国际上被列为专门的保护项目,而处于潮湿环境下的古代土遗址保护的课题在文化遗产实物和遗迹就地保存和展示领域就更为迫切。浙江良渚文化是中国新石器时代长江流域最重要的史前文化, 塘山遗址位于良渚文化遗址的西北部,选择了PS（硅酸钾）、WD－10（十二烷基三甲氧基硅烷）、WD－S（低聚甲氧基硅烷）、RTV（聚有机硅氧烷+硅酸乙酯）4种保护材料对塘山遗址进行了保护加固实验研究。室内实验和现场实验（2个月,6个月,9个月）的结果都表明,RTV和WD-10防水性优于WD-S,WD-10和WD-S材料仅能作为防水材料,PS材料仅能作为加固材料使用,而RTV则具有较好的加固和防水效果,且加样量越大,强度提高越多。  相似文献