东海原甲藻cDNA文库构建及尝试性EST分析   总被引：2，自引：0，他引：2  

张秀芳  杨官品  刘永建  李瑞香  朱明远 《中国海洋大学学报(自然科学版)》2006,36(3):361-364

东海原甲藻(Prorocentrumdonghaiense)是中国沿海频繁发生的大规模赤潮的原因种之一,大规模分离鉴定东海原甲藻功能基因是理解东海原甲藻赤潮形成过程和机理的重要基础。取对数生长期藻体,经微量总RNA抽提、cDNA合成、cDNA扩增和克隆等步骤,构建了东海原甲藻cDNA文库并进行了尝试性表达序列标签分析。从含有约5000个转化子的文库中随机取150个测序,获得126条EST序列。经网上BlastN及BlastX分析,共发现11个是已知功能的基因的标签。这些基因与东海原甲藻生长发育、物质转换和能量代谢相关。  相似文献   

青岛胶州湾3.2级地震构造背景与控震断裂   总被引：1，自引：0，他引：1  

栾光忠  刘红军  刘冬雁 《中国海洋大学学报(自然科学版)》2002,32(5):763-769

本文应用构造分析的方法 ,对青岛胶州湾 3.2级地震发生的地震地质背景和构造背景进行分析。结果表明 :胶州湾 3.2级地震主要受 NEE向郝官庄断裂和 NNW向大沽河断裂控制 ,并根据现代活动断裂的标志 ,对上述 2组断裂活动性特征作了阐述  相似文献   

Hydrocarbon Potential of Pre-cenozoic Strata in the North Yellow Sea Basin     

蔡峰戴春山  陈建文李刚 孙萍 《海洋通报(英文版)》2005,7(1):21-36

The North Yellow Sea Basin ( NYSB ), which was developed on the basement of North China (Huabei) continental block, is a typical continental Mesozoic Cenozoic sedimentary basin in the sea area. Its Mesozoic basin is a residual basin, below which there is probably a larger Paleozoic sedimentary basin. The North Yellow Sea Basin comprises four sags and three uplifts. Of them, the eastern sag is a Mesozoic Cenozoic sedimentary sag in NYSB and has the biggest sediment thickness; the current Korean drilling wells are concentrated in the eastern sag. This sag is comparatively rich in oil and gas resources and thus has a relatively good petroleum prospect in the sea. The central sag has also accommodated thick Mesozoic-Cenozoic sediments. The latest research results show that there are three series of hydrocarbon source rocks in the North Yellow Sea Basin, namely, black shales of the Paleogene, Jurassic and Cretaceous. The principal hydrocarbon source rocks in NYSB are the Mesozoic black shale. According to the drilling data of Korea, the black shales of the Paleogene, Jurassic and Cretaceous have all come up to the standards of good and mature source rocks. The NYSB owns an intact system of oil generation, reservoir and capping rocks that can help hydrocarbon to form in the basin and thus it has the great potential of oil and gas. The vertical distribution of the hydrocarbon resources is mainly considered to be in the Cretaceous and then in the Jurassic.  相似文献   

中国花鲈细胞色素b基因片段的序列研究(英文)     

高天翔  张秀梅  陈大刚  张美昭  任一平  张亚平 《中国海洋大学学报(自然科学版)》2001,31(2)

参考鳗鲡等鱼类线粒体 DNA序列进行了中国花鲈线粒体 DNA细胞色素 b基因片断的引物设计、PCR扩增及其序列测定。得到中国花鲈的碱基序列为 4 10 bp,其 A、T、G、C含量分别为 10 1bp(2 4 .6 3% )、112 bp(2 7.32 % )、72 bp(17.56 % )、12 5bp(30 .4 9% ) ,与鳗鲡等其他鱼类相同基因片断序列碱基含量相似。  相似文献   

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri     

YU Ziniu  WEI Xiaohu  KONG Xiaoyu  YU Shanshan 《海洋学报(英文版)》2007,26(1):93-100

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   

西沙礁序列的磁性地层学研究   总被引：3，自引：0，他引：3  

张明书  周墨清 《海洋地质与第四纪地质》1996,16(3):61-65

利用西沙群岛西琛一井３３０ｍ以上井段的１４９块岩心标本，用美国２－Ｇ型超导磁力仪进行了古地磁测定，获得了一个从更新世至中新世晚期包括布容，松山，高斯和吉尔伯特四个极性时及若干亚极性的磁性地层柱，为礁序列的划分对比，提供了新的依据。  相似文献   

美国东南部大陆边缘的天然气水合物利用宽角地震资料进行全波形约束和走时反演     

J.Korenaga  张光学 《海洋地质》2002,(1):21-44

利用BSR已完成美国东南边缘大约50000km2的地区编图，并已证实可能与大量天然气水合物有关。1992年6月，同时获得该地区的单道地震和宽角海底地震资料，主要集中在布莱克洋脊和卡罗来纳海隆，能清楚地观察到来自BSR的宽角反射偏移距（达6km）。为了了解区域性的变化和生成卡罗纳海隆高背景速度的二维平均速度模型，用宽角和垂直入射资料进行走时反演，然后做全波形反演业确定BSR的地震成因。最合适的模型显示了两处的BSR之下具相似的低速（1.4km/s），暗示圈闭了低饱和度（10％）的游离气，反演结果也显示有一薄层高速楱状体，最大速度为2.3km/s，刚好位于布莱克洋脊的BSR上方；计算了沉积物反射率，观察到卡罗来纳海隆具有较高的反射率，BSR之下的反射率增加几乎与两个站位的含气带对应。根据速度模型估算了水合物的浓度。另一方面，占沉积物总体积3%的水合物之平均浓度暗示布莱克洋脊地区水合物稳定带厚度少一半。卡罗来纳海隆水合物平均 浓度最大为20%，卡罗来纳海隆7%。这两处水合物浓度高，无法仅用原地生物活动来解释，可能与次生的聚集机制不关，因稳定域迁移引起水合物循环，可能影响两地的水合物凝聚。另外，流体向上排出的增加，也可以使 布莱克洋脊生存下去。  相似文献   

时间序列分析的一种新方法     

潘增宪 《海洋信息》1993,(11):20-21

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4-溴苯甲酸-2,3,4,5,6-五溴苯酯的合成(英文)     

钱佐国  王燕君  孙明昆 《中国海洋大学学报(自然科学版)》1992,(3)

以苯酚和4—溴苯甲酸为原料,合成一种尚未见文献记载的新化合物——4—溴苯甲酸—2,3,4,5,6—五溴苯酯,用无水三氯化铝催化苯酚的全溴化。此产物可望在阻燃剂方面得到应用。  相似文献   

Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences     

WANG Jun  ZHANG Wen  SU Yongquan  DING Shaoxiong 《海洋学报(英文版)》2004,23(4):709-716

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.  相似文献