干旱区荒漠植被丙二醛及保护酶活性对地下水位的响应     

庄丽  陈亚宁  李卫红  袁素芬 《冰川冻土》2005,27(5):723-733

塔里木河下游的乔木、灌木、草本植被的主要建群种为胡杨、柽柳和罗布麻,对其中丙二醛(MDA)含量以及保护酶活性对地下水位的研究表明:1)MDA含量以及SOD、POD活性均呈增加趋势,而且均与地下水位呈正相关关系;2)对同一断面相同地下水位条件下胡杨、柽柳和罗布麻的SOD活性和POD活性比较,可以反映出SOD和POD作用的互补性;3)根据不同地下水位条件下胡杨、柽柳和罗布麻的MDA含量和SOD活性所反映出的适宜植物生存的合理生态水位、(严重)胁迫水位,并将其进行综合比较与分析,初步推断在塔里木河下游地下水埋深超过3.12 m时,胡杨和柽柳的生长即开始受到抑制,6 m左右的地下水位即对胡杨造成严重胁迫,≥8.83 m的地下水位则会危及胡杨的生存;而柽柳的严重胁迫地下水位以及临界地下水位分别为5 m左右和≥8.83 m.相比之下,罗布麻所能承受的胁迫地下水位比较高,超过3.12 m的地下水位即会对其生长造成严重水分胁迫,地下水位低于4.42 m则危及其生存.  相似文献   

水杨酸通过提高抗氧化酶系统活力增强拟南芥悬浮细胞的耐寒性     

石玉兰  陈宣  岳君  安黎哲  李新荣  陈国雄 《冰川冻土》2010,32(6)

与未经水杨酸(SA)处理的低温对照相比,0.1 mmol.L-1SA处理提高了拟南芥悬浮细胞在整个低温胁迫期间超氧化物歧化酶(SOD),过氧化物酶(POD)和过氧化氢酶(CAT)的活性,并在低温胁迫初期提高了细胞抗坏血酸过氧化物酶(APX)的活性.在抗氧化酶系统活力提升的同时,还伴随着相对电导率的降低和丙二醛(MDA)含量的减少,即细胞膜状态的恢复.相关性研究显示,膜状态的恢复与抗氧化酶系统中各类酶活力的增加呈显著正相关.上述结果表明,SA可以通过提高拟南芥悬浮细胞的抗氧化酶系统活力避免氧化损伤,从而协助膜系统在低温胁迫下的自我恢复,使细胞抗寒性得以增强.  相似文献   

Enzyme responses and lipid peroxidation in gills and hepatopancreas of clam Mactra vereformis, following cadmium exposure     

������  �����  �����  ���� 《中国海洋湖沼学报》2011,29(5):981-989

To assess the toxicity of heavy metal pollution to marine intertidal shellfish, enzymatic responses and lipid peroxidation were investigated in the clam Mactra vereformis exposed to cadmium under laboratory conditions. Three antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPx), two immune defense enzymes (acid phosphatase, ACP; alkaline phosphatase, ALP), and one lipid peroxidation product (malondialdehyde, MDA) were measured in the gills and the hepatopancreas of the ...  相似文献   

长石莼（缘管浒苔）（Ulva linza）原生质体再生与分化发育初步研究          下载免费PDF全文

陈群芳  汤文仲  冯子慧  霍元子  何培民 《海洋与湖沼》2011,42(3):397-403

采用细胞酶解技术获得长石莼（缘管浒苔）原生质体,再通过细胞培养观察原生质体再生以及各种分化发育途径。结果表明,长石莼（缘管浒苔）主要存在3种分化发育途径：1）部分原生质体可直接分裂形成有假根或无假根得单细胞苗。2）部分原生质体分裂成规则细胞团或不规则细胞团,最后形成苗簇。3）部分原生质体可发育为孢子囊／配子囊,其中部分...  相似文献   

In vitro Acute Cytotoxicity of Abamectin to the Gill Cell Line of Flounder Paralichthy olivaceus     

XU Yuyan GUO Huarong XIAO Qin SU Feng YIN Licheng 《中国海洋大学学报(英文版)》2007,6(4):369-372

The cytotoxicity of abamectin to the Gill Cell Line of Flounder (FG cell line) was examined in this study. It was found that the exposure of FG cells to abamectin caused the decreases of both cell growth rate and antioxidant enzyme activities,and the increase of intracellular O2- content. It was proposed that the reduction of antioxidant enzyme activities in FG cells caused the accu- mulation of O2- content in FG cells, leading to the change of cell morphology and even the death of cells. The results showed that FG cell line is suitable for the evaluation of the acute toxicity of abamectin.  相似文献   

温度和盐度对军曹鱼幼鱼生长与抗氧化酶活性的影响   总被引：2，自引：0，他引：2  

杨健  陈刚  黄建盛  张健东  施钢  汤保贵  周晖 《广东海洋大学学报》2007,27(4):25-29

在实验室条件下,采用两因素交叉分组的方法,初步研究了环境因子(温度和盐度)对军曹鱼幼鱼肌肉抗氧化酶比活力的影响。温度设3个水平(26、29、32),盐度设4个水平(11、19、27、35),两个因素按其各自水平共产生12种组合。军曹鱼幼鱼在玻璃钢水槽内养殖20d后取样测定超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPX)的比活力。结果表明:盐度因子诱导了军曹鱼的抗氧化应激反应,抗氧化酶活力随盐度降低而升高,SOD、CAT和GPX比活力分别从35时的17.74±3.38、19.44±2.66和13.70±2.99U/mg上升到11时的30.14±1.76、35.63±10.59和17.44±4.32U/mg;温度、盐度及因素间的交互作用对SOD和CAT比活力有显著影响(P<0.05);温度对GPX比活力无显著影响(P>0.05),而盐度及因素间的交互作用对GPX比活力影响显著(P<0.05)。  相似文献   

Adaptation of the antioxidant defence system in hydrothermal-vent mussels (Bathymodiolus azoricus) transplanted between two Mid-Atlantic Ridge sites     

Rui Company  Angela Serafim  Richard Cosson  Aline Fiala-Médioni  David R. Dixon  & Maria João Bebianno 《Marine Ecology》2007,28(1):93-99

The vent mussel Bathymodiolus azoricus is the dominant member of the Northern Mid‐Atlantic Ridge (MAR) hydrothermal megafauna, and lives in an environment characterized by temporal and spatial variations in the levels of heavy metals, methane and hydrogen sulphide, substances which are known to increase reactive oxygen species levels in the tissues of exposed organisms. To evaluate the effects of two contrasting hydrothermal environments on the antioxidant defence system of this vent mussel species, a 2‐week transplant experiment was carried out involving mussels collected from the relatively deep (2300 m), and chemical rich, Rainbow vent field. These were transplanted to the shallower (1700 m), and relatively less toxic, Lucky Strike vent field. To achieve this objective, levels of superoxide dismutase, catalase (CAT), total glutathione peroxidase (GPx), selenium‐dependent glutathione peroxidase and lipid peroxidation (LPO) were measured in the gills and mantle tissues of resident and transplant mussels before and after the transplant experiment. With the exception of CAT, the gills of the transplanted mussels had significantly higher antioxidant enzyme activity compared with the basal levels in the donor (Rainbow) and recipient (Lucky Strike) populations; whereas the antioxidant enzyme levels in the mantle tissues of the transplants reflected the baseline levels of activity in the native Lucky Strike mussels after 2 weeks. In contrast, LPO levels were significantly higher in both tissue types in the transplants than in either the source or the recipient populations, which suggested a response to hydrostatic pressure change (note, the transplant animals were brought to the surface for transportation between the two vent fields). The fact that the Rainbow mussels survived the transplant experience indicates that B. azoricus has a very robust constitution, which enables it to cope behaviourally, physiologically and genetically with the extreme conditions found in its naturally contaminated deep‐sea environment.  相似文献   

Are metal-induced hypersensitivities in harbor seals associated with liver function?     

Kakuschke A  Valentine-Thon E  Griesel S  Fonfara S  Siebert U  Prange A 《Marine pollution bulletin》2011,62(8):1891-1894

Environmental exposure to metals is believed to affect marine mammal health adversely including immunosuppression or acute as well as chronic inflammatory processes leading to hypersensitivities or autoimmune diseases. Metal-specific hypersensitivities were found in several pinnipeds of the North Sea. However, hypersensitivity is a complex phenomenon whose characteristics are still not completely understood; in particular, effects on health are not well established. In the present study, we compared basic hematological and biochemical parameters of seals with and without metal-specific hypersensitivities. We found altered hematological parameters and liver enzyme patterns in seals with a metal-induced hypersensitivity, including a reduction in macrophages, an increase in lymphocytes, and elevated levels of lactate dehydrogenase. These findings support the suggestion of a chronic influence of metal pollutants on the health of marine mammals of the North Sea.  相似文献   

斜带石斑鱼幼鱼对羟基蛋氨酸铜的需求     

马豪勇  杨奇慧  董晓慧  谭北平  迟淑艳  刘泓宇 《广东海洋大学学报》2014,(4):19-26

在斜带石斑鱼(Epinephelus coioides)幼鱼基础饲料(铜质量分数2.03 mg/kg)中分别添加6个水平的羟基蛋氨酸螯合铜(hydroxy methionine copper,HMC),制成铜质量分数分别为2.03、4.04、6.20、10.67、13.96 mg/kg的实验饲料,饲喂6周后,探讨HMC对幼鱼生长、非特异性免疫酶活性和组织铜沉积的影响,确定幼鱼饲料中铜的最适需要量。结果表明,饲料中添加HMC,对石斑鱼的增重率、特定生长率(SGR)和饲料系数等生长性能的影响有统计学意义(P0.05),以铜质量分数6.20 mg/kg组增重率和特定生长率最高,饲料系数最低,高于或低于2.03和13.96 mg/kg组,差异有统计学意义(P0.05),与4.04和10.67 mg/kg组差异无统计学意义(P0.05);但对成活率、肝体比影响差异无统计学意义(P0.05)。饲料中添加HMC对全鱼水分和粗蛋白含量影响无统计学意义(P0.05),对全鱼粗灰分和粗脂肪含量影响有统计学意义(P0.05)。石斑鱼血浆铜蓝蛋白(CP)的含量、血清和肝脏总超氧化物歧化酶(T-SOD)及铜锌超氧化物歧化酶(Cu-Zn-SOD)活性均以6.20 mg/kg组最高;饲料中铜的添加对血清谷胱甘肽过氧化物酶(GSH-Px)活性和肝脏硫代巴比妥酸反应物(TBARS)含量影响无统计学意义(P0.05)。与对照组相比,饲料中添加HMC均提高鱼体、肌肉和肝脏中铜含量,差异有统计学意义(P0.05),且以13.96 mg/kg组最高。饲料中添加适量的HMC可提高石斑鱼幼鱼的生长性能,提高抗氧化能力。在本实验条件下,以SGR为判据,通过二次回归方程拟合可得,以HMC为铜源,斜带石斑鱼幼鱼铜需要量为7.09 mg/kg。  相似文献   

海草抗氧化系统及其对逆境胁迫的响应特征     

卢科宇  裴炎沼  何晴  周斌 《海洋科学》2022,46(8):171-185

海草具有独特的进化地位和重要的生态价值,广泛分布于潮间带和潮下带浅海海域,易遭受多种环境因子变化的威胁,抗氧化系统在海草抵御逆境胁迫的过程中具有非常重要的作用。本文综述了海草抗氧化系统的组成、特征及其对主要逆境胁迫的响应特征研究进展,阐述了海草主要酶促抗氧化机制,并将北半球代表性海草物种——鳗草抗氧化系统相关酶基因归类分析。目前对于海草逆境胁迫的研究主要集中于单一胁迫下主要抗氧化酶(如SOD、CAT、GST)及其转录组的变化,对于多胁迫因子协同作用和非酶抗氧化物及其他抗氧化酶的响应特征研究较少;另外对于逆境胁迫下不同海草种类间抗氧化系统和关键基因的响应差异也有待深入研究。  相似文献