Microbial Community Structures in Petroleum Contaminated Soils at an Oil Field,Hebei, China          下载免费PDF全文

Yizhi Sheng  Guangcai Wang  Chunbo Hao  Qian Xie  Qian Zhang 《洁净——土壤、空气、水》2016,44(7):829-839

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海洋微生物在船舶用结构钢表面附着成膜过程及其腐蚀研究   总被引：1，自引：0，他引：1  

董耀华  贺中意  郭娜  刘涛  董丽华 《海洋学研究》2015,33(1):39-44

通过紫外分光光度计测定了海洋微生物需钠弧菌 Vibrio natriegens 的生长曲线,通过扫描电镜和原子力显微镜观测了该细菌在船舶用结构钢(DH32)钢样表面成膜过程及试样腐蚀形貌,探讨了生物膜的形成过程及其对材料表面腐蚀的影响。结果表明,生物膜的形成过程与微生物生命活动关系密切。根据对比暴露在菌液和无菌培养基中的试样表面形貌,发现细菌的附着及成膜过程的不均匀性,改变了DH32钢样表面的物理和化学状态。细菌附着区与周围形成的氧浓差电池,以及细菌新陈代谢主要产物对金属离子的络合,共同促进了试样局部腐蚀加速。  相似文献   

肠道细菌对厚壳贻贝稚贝附着的作用研究   总被引：1，自引：0，他引：1  

杨娜  梁箫  彭莉华  沈和定  杨金龙 《海洋科学》2017,41(11):45-54

为探讨肠道细菌形成的微生物被膜对贝类附着的作用,从厚壳贻贝(Mytilus coruscus)成体肠道中分离10株肠道细菌,调查厚壳贻贝肠道细菌形成微生物被膜特性,分析微生物被膜密度、细菌种属与厚壳贻贝稚贝附着之间的相互关系。所有肠道细菌均可有效促进稚贝附着。其中,Paracoccus sp.1微生物被膜诱导的稚贝附着率最高(61%),且微生物被膜膜厚较厚,细菌分布较密集;仅5株肠道细菌Roseovarius sp.1、Winogradskyella sp.1、Tenacibaculum sp.4、Bacillus sp.5和Nonlabens sp.1的被膜密度与附着显著相关(P0.05)。系统发育分析发现肠道细菌诱导附着率与其种属无关。本研究表明源于厚壳贻贝成贝肠道细菌可以促进其稚贝的附着,说明肠道细菌对厚壳贻贝的生长发育具有一定的作用。本研究结果为深入探讨肠道细菌与厚壳贻贝相互作用和推动该种规模化养殖提供理论依据。  相似文献   

Ternary interactions in a humic acid–Cd–bacteria system     

Peter G. Wightman  Jeremy B. Fein 《Chemical Geology》2001,180(1-4):55-65

Humic acid adsorption onto the bacterial surface of Bacillus subtilis was measured with and without Cd, as a function of pH and humic–bacteria–Cd ratios. These experiments tested for the existence of ternary interactions in a bacteria–humic–metal system. We determine both the effects of humic acid on the bacterial adsorption of Cd, as well as the effects of the aqueous metal cation on the bacterial adsorption of humic acid. The presence of Cd does not affect the extent of humic acid adsorption onto the bacterial surface, indicating that there is no competition for sorption sites between humic acid and Cd under the experimental conditions, and that changes in the charging properties of the bacterial surface, as a result of the Cd adsorption, are not significant enough to affect humic acid adsorption.

The presence of humic acid does diminish Cd adsorption onto the bacterial surface, suggesting the presence of an aqueous Cd–humate complex under mid to high pH conditions. However, we also observe that the solubility of humic acid is unaffected by the presence of aqueous Cd. This apparently inconsistent behavior of an aqueous Cd–humate complex affecting Cd adsorption but not affecting humic acid solubility is not observed with simpler ionizable organic molecules. We propose that the solubility of humic acid is controlled by the solubility of a less soluble fraction of the acid. Cd forms an aqueous complex with the more soluble fraction of humic acid and there is no interdependence between the aqueous activities of the more and less soluble fractions. That is, the solubility of one humic acid fraction is unaffected by the presence of an aqueous Cd–humate complex involving another humic acid fraction. These experimental results constrain the relative importance of surface ternary and aqueous metal–humate complexes on the bacterial adsorption of both humic acid and metal cations.  相似文献   

Impact of anthropogenic activities on the sediment microbial communities of Baiyangdian shallow lake   总被引：1，自引：0，他引：1  

《国际泥沙研究》2020,35(2):180-192

Microorganisms are recognized as sensors of external disturbance in freshwater ecosystems.In this study,sediment samples from regions with strong human activity interference(four sites),moderate human activity interference(five sites) and minor human activity interference(five sites) in Baiyangdian Lake were collected;and their physiological and chemical properties and heavy metals contents were analyzed.The structures of bacterial and fungal communities were tested using 16 S ribosomal Ribonucleic Acid(rRNA) and Internal Transcribed Spacer 2(ITS2) high-throughput sequencing technologies.The results indicated that Proteobacteria was the most abundant bacterial phylum.Ascomycota,Basidiomycota and Ciliophora were the dominant phyla of fungi.Obvious differences of microbial genera among the three regions we re obse rved,especially fungal genera.With intensified disturbance by human activities,the Simpson and Shannon indices of bacteria significantly decreased.The fungal communities were clustered into three groups in accordance with the different human disturbance levels.The bacterial communities of sediment samples under moderate and minor human disturbance appeared similar,but different from those under strong human disturbance.Redundancy analysis indicated that zinc,total phosphorus,copper,and lead were the dominant factors that influenced the bacterial community.Nitrifiers and sulfate-reducing bacteria were influenced by pH,nitrate(NO3),and total nitrogen.The fungal community was significantly affected by total phosphorus.This study reveals that sediment microbial community structures among different regions of a shallow lake are mainly impacted by human activities.  相似文献   

Microbial fossilization in carbonate sediments: a result of the bacterial surface involvement in dolomite precipitation   总被引：14，自引：1，他引：14  

Yvonne Van Lith  Rolf Warthmann  Crisogono Vasconcelos  Judith A. McKenzie 《Sedimentology》2003,50(2):237-245

ABSTRACT Recent dolomitic sediment samples from Lagoa Vermelha, Brazil, were examined microscopically to study the process of bacterial fossilization in carbonate sediments. Bacteria‐like bodies were intimately associated with carbonate mineral surfaces, and coatings on the former demonstrate the calcification of single bacterial cells. The bacterial fossilization process in Lagoa Vermelha sediments was simulated in the laboratory by cultivation of mixed and pure cultures of sulphate‐reducing bacteria, which were isolated from the Lagoa Vermelha sediments. These cultures produced carbonate minerals that were studied to provide insight into the initiation of the fossilization process. In mixed culture experiments, bacterial colonies became calcified, whereas in pure culture experiments, single bacterial cells were associated with dolomite surfaces. Dolomite nucleated exclusively in bacterial colonies, intimately associated with extracellular organic matter and bacterial cells. Electrophoretic mobility measurements of the bacterial cells in electrolyte solutions demonstrated the specific adsorption of Ca²⁺ and Mg²⁺ onto the cell surfaces, indicating the role of the bacterial surface in carbonate nucleation and bacterial fossilization. The affinity of the cells for Mg²⁺ was related to the capability of the strains to mediate dolomite formation. Combined with sulphate uptake, which dissociates the [MgSO₄]⁰ ion pair and increases the Mg²⁺ availability, the concentration of Mg²⁺ ions in the microenvironment around the cells, where the conditions are favourable for dolomite precipitation, may be the key to overcome the kinetic barrier to dolomite formation. These results demonstrate that bacterial fossilization is a consequence of the cell surface involvement in carbonate precipitation, implying that fossilized bacterial bodies can be used as a tool to recognize microbially mediated carbonates.  相似文献   

Groundwater inflow and associated transport of phosphorus to a hypereutrophic lake   总被引：2，自引：0，他引：2  

W.-J. Kang  K. V. Kolasa  M. W. Rials 《Environmental Geology》2005,47(4):565-575

Hydrogeochemical data from lake, sediment pore, and well waters were used to quantify groundwater seepage and the associated transport of phosphorus to Lake Persimmon, Florida, USA. The data show that lake chloride concentrations vary as a function of lake elevations that are controlled by groundwater inflow. A whole-lake average seepage rate, estimated using a simple one dimensional advection-diffusion model fitted to the lake chloride profile, currently averages 2.3 ± 0.3 cm yr^-1 and is in reasonable agreement with the rate of advective flow obtained from the pore water chloride profile. The ratios of nutrient regeneration versus sulfate consumption indicate that the phosphorus enrichment in deeper portions of sediment pore water is most likely a result of groundwater phosphorus transport through sediment. Thus, the net inputs of groundwater phosphorus to the lake, calculated using the deep pore water phosphorus concentration, are about 7.4 ± 4.3 mg P m^-2 yr^-1 and comparable with recent in situ estimates from seepage meters. This study provides a simple hydrogeochemical method for estimating hydrologic and phosphorus inputs via groundwater to the lake, thereby supporting current efforts for lake management.  相似文献   

Benthic microbial abundance and activities in an intensively trawled ecosystem (Thermaikos Gulf, Aegean Sea)     

Paraskevi N. Polymenakou  Antonio Pusceddu  Anastasios Tselepides  Thalia Polychronaki  Antonia Giannakourou  Carla Fiordelmondo  Eleni Hatziyanni  Roberto Danovaro 《Continental Shelf Research》2005,25(19-20):2570

Abundance of benthic bacteria, heterotrophic nanoflagellates and ciliates, extracellular enzymatic activities, bacterial C production, C mineralisation and sediment community oxygen consumption rates were measured in the Thermaikos Gulf (Northeastern Mediterranean), before (September 2001), and during intense trawling activities (October 2001 and February 2002). The biochemical composition of sedimentary organic matter has revealed that bottom trawling had an effect on the trophic state of Thermaikos Gulf. Changes on the benthic microbial food web were also recorded, during the three sampling seasons. Even though trawling-induced sediment resuspension did not alter significantly the abundance of the microbial components, with the exception of the most impacted station, it determined changes regarding their relative importance. Thus, the ratios of bacterium to nanoflagellates and ciliate to nanoflagellates abundance increased in the trawled stations, causing a sudden increase in bacterial C production, in comparison to the non-trawled station. Four months later, the effects of trawling on the microbial food web were less evident, masked possibly by the drastic decrease in the water temperature. The results of the present work suggest that bottom trawling induces alteration of the sedimentological variables and can be considered as a factor affecting the function of the microbial food web in marine coastal ecosystems. These alterations cause faster mobilisation of organic C buried in the sediment and increase nutrient concentrations and availability in the system, thus inducing an effect that could lead to coastal eutrophication.  相似文献   

Bacterioplankton and phytoplankton production in seven lakes in the Middle Rio Doce basin, south-east Brazil     

Mauricio M. Petrucio  Francisco A.R. Barbosa 《Limnologica》2006,36(3):192-203

The purpose of this study was to measure the phytoplankton production (PP) and bacterial production (BP) simultaneously in seven lakes in the middle Rio Doce basin (southeast Brazil) during the dry and the rainy seasons. Limnological monitoring was conducted from 1999 to 2001 as well as both PP (radioactive carbon fixation) and BP (³H-Leucine incorporation) in four specific depths (100%, 10%, 1% of incident radiation, and aphotic zone). Furthermore, trophic state of the lakes was analyzed, considering the index proposed by Salas and Martino (1991) and Carlson (1977). In general, some parameters increased during the rainy season such as pH, conductivity, chlorophyll and total nitrogen. In the period of 1999/2000, the rain caused PP reduction in the lakes, excepting Carioca and Águas Claras Lake, but in the years 2000/2001, the lakes did not show a similar pattern. In contrast, the rainy season of both years caused an expressive bacterioplankton production enhancing. Using Salas and Martino index, we observed that some lakes were affected by the seasonal runoff, therefore the typologie of these lakes changed from oligotrophic to mesotrophic conditions, otherwise considering the Carlson index all lakes were classified as eutrophic in both dry and rainy seasons. Indeed, Salas and Martino index demonstrated to be more appropriate to determine the trophic status of the lakes. Finally, our data indicate that until now, in spite of the lakes location, protected and unprotected area, the lakes still maintain their natural characteristics.  相似文献   

重庆二叠系-三叠系界线地层微生物岩新认识   总被引：2，自引：0，他引：2  

姜红霞  吴亚生 《岩石学报》2007,23(5):1189-1196

通过对重庆老龙洞二叠系-三叠系界线附近的微生物岩的研究,认为微生物岩的树枝状构造的形成有两种控制因素:(1)成岩作用,成岩流体对碳酸盐沉积物颗粒之间的孔隙及化石内部的孔隙的胶结作用,以及碳酸盐沉积物组分的重结晶作用,使受成岩作用影响的区域因晶体较粗在露头上表现为深颜色的树枝状和斑点状构造;(2)微生物的影响。在微生物富集的地方,微生物死亡腐烂后形成孔隙,成岩流体易于在这些区域运移,在孔隙中形成胶结物,使这些区域成为露头上颜色较深的树枝状和斑点状构造。岩石中的微生物化石形态不规则,在薄片上呈蝴蝶状、云朵状、不规则圆形的或者新月形等形状,大小在100～600μm之间,内部被方解石胶结物充填,没有保存任何内部构造。根据形态和大小,推测这种化石可能是某种细菌的菌落形成的。  相似文献