Optimization of fluorescence in situ hybridization （FISH） for the identification of two polar coccoid green algae species     

高小艳  李运广  李会荣  陈雯莉  罗玮 《极地研究(英文版)》2010,21(2):167-179

Standard FISH protocols using fluorochrome-labeled oligonucteotide probes have been successfully applied for in situ detection.However,optimized protocols of FISH for specific eukaryotes in marine environments are often not developed.This study optimized the conditions of fluorescence in situ hybridization (FISH) by using two polar isolated microalgae.The modified conditions were as follows: (1) 10 mg·mL-1 lysozyme solution pretreatment at 37℃ for 30 min;(2) the hybridization buffer including 20% formamide;(3) the hybridization condition was 47℃ for 6 h. The cells enumerated by FISH were compared with those enumerated by flow eytometry (FCM) and DAPI to confirm the cell loss and hybridization efficiency.The optimized protocol was also successfully applied to Arctic Ocean samples,which were found to be dominated by Micromonas sp.The modified protocol showed a high relative efficiency and could be successfully applied for the detection of specific microbial eukaryotes in environmental samples.  相似文献   

Demographic parameters and mixis of three Brachionus angularis Gosse (Rotatoria) strains fed on different algae     

Haoyuan Hu  Yilong Xi   《Limnologica》2008,38(1):56-62

Rotifers are one of the smallest metazoans, served as perfect material for evolution theories and excellent food resources to larva in aquaculture. Population dynamics of rotifers help to optimize culture conditions, and to understand selection of asexual and sexual reproduction types. We described population dynamics of three Brachionus angularis strains using the life table method, with three types of food. The following demographic parameters were analyzed: intrinsic rate of increase (r), net reproductive rate (R₀), generation time (T), average lifespan (L), and the total percentage of mictic females in the offspring. The results showed that there were significant effects of strain on the parameters except for R₀. However, the food type significantly affected r, R₀, T, and the total percentage of mictic females in the offspring. Demographic parameters differed among strains, showing the possible adaptation to local niches. In comparison to Chlorella pyrenoidosa, Scenedesmus obliquus served more nutrition to enlarge population size, and more mixis was induced in rotifer populations fed on it.  相似文献   

扇形游仆虫对细菌及小球藻摄食的初步探讨     

马洪钢  张绍丽  宋微波 《中国海洋大学学报(自然科学版)》2000,30(3):423-427

选用大肠杆菌及小球藻投喂海洋腹毛目纤毛虫扇形游仆虫 ,对其摄食习性、种群自然增长率及其间的能量转化进行了观察和探讨。结果显示 :游仆虫摄食细菌和小球藻的种群自然增长率分别为 0 .6 4 /d和 0 .37/d,其能量转化率分别为 4 4 .2 %和 2 1.6 % ;在适宜密度的细菌和小球藻存在条件下 ,游仆虫能迅速形成较高的增长率 ,而不出现明显的诱发生长期。  相似文献   

太湖短吻银鱼的胚胎发育   总被引：17，自引：0，他引：17  

张开翔 《湖泊科学》1998,10(1):55-61

椭圆卵囊藻培养轮虫时,轮虫平均产卵量均低于４．０个,随着食物浓度增加,后代混交雌体百分率降至１２．５０％．投喂蛋白核小球藻,轮虫的平均产卵量从６．００增加到１１．４０个,后代混交雌体百分率降至４．５８％．小球藻和卵囊藻混合培养轮虫,轮虫平均产卵量少于５．００个,后代个体混交百分率下降．当小球藻浓度增加到８．０＊１０（６）ｃｅｌｌｓ／ｍｌ时,平均产卵量下降至８．３３个,混交百分率也明显升高。  相似文献   

铜绿微囊藻(Microcystis aeruginosa)和蛋白核小球藻(Chlorella pyrenoidosa)生长及光合活性对温度和光照交互作用的响应   总被引：1，自引：0，他引：1  

刘世明  朱雪竹  张民 《湖泊科学》2014,26(5):773-779

通过室内纯培养,分别设定不同温度梯度(14、16、18℃)和光照梯度(100、250、360 μmol/(m2·s)),以模拟全球气候变化条件下,温带湖泊春季温度升高与光照减弱交互作用对铜绿微囊藻和蛋白核小球藻生长和光合活性的影响.通过流式细胞计数测定其生长曲线,观测其变化趋势,并利用叶绿素荧光仪PHYTO-PAM测定藻类光合活性.结果表明,当温度为18℃,光强为100μmol/(m2·s)时,铜绿微囊藻生长速率最大,细胞密度达到2.99×106cells/ml.在该温度和光照强度下,其光合活性也远高于其他实验组,其Fv/Fm值达到最大值0.39.蛋白核小球藻在温度为16和18℃时,其生长速率差异不大,但明显高于在温度为14℃时的生长速率,其光合活性处于波动状态.与蛋白核小球藻相比,温度升高和光照减弱对铜绿微囊藻生长和光合活性的促进作用更为明显,而且随着温度的升高,铜绿微囊藻生长对低光的偏好更为显著.因此,全球变暖所导致的高温低光环境可能有利于确立铜绿微囊藻在湖泊中的优势地位.  相似文献   

椭圆小球藻( Chlorella ellipsoidea )对4种重金属的耐受性及富集   总被引：28，自引：1，他引：28  

浩云涛  李建宏  潘欣  马宇翔  王雪锋 《湖泊科学》2001,13(2):158-162

分离筛选获得了一株高重金属抗性的椭圆小球藻 (Chlorellaellipsoidea) ,并研究了不同浓度的重金属铜、锌、镍、镉对该藻生长和叶绿素a含量的影响及其对重金属离子的吸收富集作用 .结果显示 ,该藻对Zn2 +和Cd2 +具有很高的耐受性 .对四种重金属的耐受能力依次为锌 >镉>镍 >铜 .其叶绿素a含量与重金属离子浓度呈明显负相关 .该藻对重金属具有很好的去除效果 ,经 15μmol/LCu2 +、30 0 μmol/LZn2 +、10 0 μmol/LNi2 +、30 μmol/LCd2 +浓度 72h处理 ,去除率分别达到 4 0 .93%、98.33%、97.6 2 %、86 .88% ,可应用于含重金属废水的处理  相似文献   

自养小球藻硫酸多糖的红外光谱分析     

梁燕茹  李文权  刘四光 《海洋科学》2007,31(11):1-4

利用二乙胺基乙基琼脂糖凝胶柱层析纯化自养小球藻(Chlorella autotropica)粗多糖,得到3个硫酸多糖组分(A,B,C);利用高效液相色谱仪和熔点仪测定了3个多糖的分子质量和熔点;利用BaCl2浊度法测定了硫酸多糖中硫酸根的质量分数;利用傅立叶变换红外光谱分析了海水自养小球藻3个硫酸多糖组分的环状结构、半缩醛羟基构型及取代基类型。结果显示,3个硫酸多糖组分的硫酸根的质量分数分别为0.77%,8.46%,2.69%,3个组分均含有硫酸基、酰氨基,葡萄糖是3个硫酸多糖组分的主要组成单糖;3个硫酸多糖组分组成单糖的环构型均是吡喃环,成苷的半缩醛羟基构型有一定差异,A是α构型,B、C是α、β两种半缩醛羟基构型并存。为深入研究自养小球藻硫酸多糖的结构及抗癌活性提供基础。  相似文献   

塔玛亚历山大藻对两种单细胞藻生长的影响   总被引：7，自引：0，他引：7  

周立红  王大志 《台湾海峡》2004,23(1):43-50

本文研究了塔玛亚历山大藻(ATCI01品系)细胞再悬浮液、细胞破碎液、上清液、藻壳液、过滤液对亚心形扁藻悬浮性的影响,以及塔玛亚历山大藻与亚心形扁藻和小球藻之间的相互作用.结果发现,含有塔玛亚历山大藻成分的实验溶液均能在短时间内造成亚心形扁藻失去鞭毛形成胞囊而下沉,其中再悬浮液的影响最大.但亚心形扁藻受到的这种不利影响,一般在一周后即开始恢复.小球藻生长几乎不受塔玛亚历山大藻的影响,混合培养水体中的小球藻生长曲线与单种培养的几乎一致,其悬浮性也无明显改变.与亚心形扁藻进行混合培养的塔玛亚历山大藻(ATCI01品系和ATHK品系)均表现出培养前期增殖曲线与单种培养类似,但在一周后混合培养的塔玛亚历山大藻的增殖曲线开始下降,这一时间恰好与扁藻细胞密度开始增加的时间相一致.而与小球藻的混合培养的塔玛亚历山大藻在培养前期与单种培养一致,后期则种群数量有所下降.  相似文献   

Food Type Effects on the Population Growth Patterns of Littoral Rotifers and Cladocerans     

C. Enríquez García  S. Nandini  S. S. S. Sarma 《洁净——土壤、空气、水》2003,31(2):120-133

Littoral rotifers and cladocerans feed on bacteria, phytoplankton, and detritus. In comparison to planktonic organisms their species diversity is high, yet little is known about their biology. In this study we present data on the functional response of the cladocerans Macrothrix triserialis and Alona rectangula, and the rotifer Brachionus macracanthus to different levels of the alga Chlorella vulgaris. Based on the incipient limiting level, we chose the food concentration and tested the population growth on four types of diets for three cladoceran species — Macrothrix triserialis, Alona rectangula, and Chydorus sphaericus — and three rotifer species — Brachionus macracanthus, Lecane quadridentata, and Platyias quadricornis. We studied growth rates of each of these species on diets of live Chlorella vulgaris, heat‐killed Chlorella vulgaris, baker's yeast (Saccharomyces cerevisiae), and field collected detritus. The incipient limiting level was around 0.9 · 10⁶ cells mL^?1 of Chlorella for the tested zooplankton species. The population growth patterns showed that, in general, the cladocerans took 25…50 d to reach their peak population densities, which ranged from 10…75 ind. mL^?11. Among the rotifers L. quadridentata and B. macracanthus had equally high growth rates on live and heat‐killed forms of C. vulgaris while P. quadricornis grew best on detritus. The growth rates ranged from 0.03…0.21 d^?1. The similarities and differences between littoral and planktonic organisms in relation to food type and availability have been discussed.  相似文献   

蛋白核小球藻(Chlorella pyrenoidosa) Rubisco活化酶基因的克隆与表达分析     

邓意龙  孙雪  徐年军  杨锐 《海洋与湖沼》2012,43(1):41-46

采用RACE技术克隆了单细胞绿藻蛋白核小球藻820Rubisco活化酶基因(rca)序列,并用荧光定量PCR方法研究了rca和Rubisco小亚基基因(rbcS)的表达规律。结果获得了1703bp的rcacDNA序列,包括66bp的5’非翻译区、1242bp的开放阅读框和395bp的3’非翻译区。序列比较和分析表明该蛋白核小球藻rca序列与其它绿藻的同源性高达78%—85%;偏好使用以G/C/T结尾的密码子;推测的RCA蛋白等电点和分子量分别为8.44和45.71kDa。荧光定量PCR结果表明随光照时间增加rca与rbcS转录表达逐渐降低;不同盐度处理下rbcS的mRNA量变化不大,而rca在37.5盐度下表达量为25盐度的2.69倍;1.0—3.0mmol/L水杨酸处理rca与rbcS表达均降低。  相似文献